Usefulness of Phase Sensitive Inversion Recovery MRI Sequence in the Detection of Cortical Lesions in Multiple Sclerosis by Rezq Ahmed Shakir, Haider Najim Al-Tameemi, Hayder Hasson, Zahraa Ayad Zahraa Ayad, Raaed Hamza Jawad, Haider Abd AlRouda Jassim

“…However, the accurate detection of cortical lesions using conventional magnetic resonance imaging sequences remains challenging. The study aims to assess the value of phase-sensitive inversion recovery sequence in the detection of cortical lesions in multiple sclerosis patients and to evaluate their relation with clinical subtypes, duration, and clinical disability of the disease. …”
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Breaking the Standard: Can Oxford Nanopore Technologies Sequencing Compete With Illumina in Protistan Amplicon Studies? by Dana Bludau, Guido Sieber, Manan Shah, Aman Deep, Jens Boenigk, Daniela Beisser

“…In addition, the applicability of the recently updated Natrix2 amplicon processing pipeline for both sequencing approaches was evaluated. While higher error rates were observed for ONT reads, especially for short reads, the full‐length ONT 18S sequences achieved greater taxonomic classification accuracy down to the species level. …”
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Stacking sequence effects on flexural stiffness, failure progression, and energy absorption in asymmetric CFRP laminates by Afsar Husain, Sanan H. Khan, Abdel Hamid I. Mourad

“…Three-point bending tests were conducted to evaluate the flexural properties of each configuration. …”
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Ongoing measles outbreak in Romania: Clinical investigation and molecular epidemiology performed on whole genome sequences. by Robert Hohan, Marius Surleac, Victor Daniel Miron, Andreea Tudor, Ana-Maria Tudor, Oana Săndulescu, Ovidiu Vlaicu, Victoria Aramă, Daniela Pițigoi, Adriana Hristea, Anca Cristina Drăgănescu, Dimitrios Paraskevis, Leontina Bănică, Dan Oțelea, Simona Paraschiv

“…Screening for samples to be sequenced was performed with a commercial kit (PrimerDesign). …”
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Racial differences in endometrial cancer genomics and outcomes using UNCseqTM targeted DNA sequencing by Meredith A. Newton, Jason D. Merker, Weida Gong, Sushant Patil, Xianming Tan, David L. Cocoran, Adam D. Pfefferle, Michele C. Hayward, Russell Broaddus, Hazel B. Nichols, Andrew F. Olshan, Bernard Weissman, Temitope O. Keku, Victoria Bae-Jump

“…Objective: To use an institution-sponsored targeted sequencing effort to characterize the genomic differences in endometrioid and serous endometrial cancers (ECs) between Black and White patients and to investigate the impact on clinical outcomes. …”
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Comparative analysis of metagenomic next-generation sequencing for pathogenic identification in clinical body fluid samples by Ning Sun, Jiaxun Zhang, Wentao Guo, Jin Cao, Yong Chen, Deyu Gao, Xinyi Xia

“…Abstract Objectives This study aims to evaluate and compare the effectiveness of metagenomic next-generation sequencing (mNGS) in identifying pathogens from clinical body fluid samples, with a specific focus on the application of microbial cell-free DNA (cfDNA) mNGS. …”
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Whole-exome sequencing for prenatal diagnosis of fetal anomalies: insights from a Jordanian cohort by Mohammad Shboul, Nadia Badaro, Reem Darweesh, Rajaa Fathallah, Mohammed El-Khateeb, Oqba Al-Kuran

“…Background Congenital anomalies are a heterogeneous disorder that occur during fetal development or infancy. Whole exome sequencing (WES) is a promising diagnostic tool for prenatal diagnosis. …”
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Combination of Sample Preservation Approaches and DNA Extraction Methods for Long‐Read Sequencing of Nudibranchs' Genomes by Inés Alberola‐Mora, Oleanna Guerra‐Font, Omar Daniel Espinoza‐Calderón, Carles Galià‐Camps, Mária Džunková

“…Three aspects of DNA quality were evaluated: total yield, fragment size distribution, and availability of DNA for amplification. …”
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Conformational Propensities of a DNA Hairpin with a Stem Sequence from the c-MYC Promoter by Arees Garabet, Iztok Prislan, Nataša Poklar Ulrih, James W. Wells, Tigran V. Chalikian

“…To mimic better the situation in the genome, we here extend our studies to a monomolecular DNA construct—a hairpin—in which complementary G- and C-rich strands featuring sequences from the promoter region of the c-MYC oncogene are linked by a dT₁₁ loop. …”
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Identification of Shared Biomarkers in Chronic Kidney Disease and Diabetic Nephropathy Using Single-Cell Sequencing by Ma JS, Yang J, Wang WC, Quan YX, Liao XN, Bai YH, Jiang HY

“…Jin-Sha Ma, Jiao Yang, Wen-Chao Wang, Yi-Xiao Quan, Xing-Na Liao, Yi-Hua Bai, Hong-Ying Jiang Department of Nephrology, The Second Hospital Affiliated to Kunming Medical University, Kunming, People’s Republic of ChinaCorrespondence: Yi-Hua Bai, Department of Nephrology, The Second Hospital Affiliated to Kunming Medical University, No. 374 Dianmian Road, Wuhua District, Kunming, 650101, People’s Republic of China, Tel +86 13658897696, Fax +86 0871 63402482, Email baiyihua@kmmu.edu.cn Hong-Ying Jiang, Department of Nephrology, The Second Hospital Affiliated to Kunming Medical University, No. 374 Dianmian Road, Wuhua District, Kunming, 650101, People’s Republic of China, Tel +86 13033371998, Email 1627248965@qq.comBackground: Chronic kidney disease (CKD) and diabetic nephropathy (DN) represent significant renal health challenges, with overlapping pathogenic mechanisms. This study evaluated shared biomarkers in CKD and DN through single-cell sequencing, aiming to identify potential diagnostic and therapeutic targets and provide new insights into their common pathogenesis.Methods: In this study, single-cell RNA sequencing was performed on nine columns of human blood samples, including three control cases, three CKD cases, and three DN cases. …”
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Molecular variants of multiple genes were revealed by whole-exome sequencing in PCOS patients with diabetes by Chenglin Wang

“…The sequencing data obtained were evaluated using a variety of bioinformatics tools. …”
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Laboratory validation of targeted next-generation sequencing assay for pathogen detection in lower respiratory infection by Jing Yang, Yan Wang, Li Yang, Jun Wu

“…Targeted next-generation sequencing (tNGS) is a rising star in microbial detection, with enormous potential. …”
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Whole genome sequencing and phylogenetic analysis of dengue virus in Central Nepal from 2022 to 2023 by Margaret Chi, Nishan Katuwal, Aastha Shrestha, Surendra Kumar Madhup, Dipesh Tamrakar, Rajeev Shrestha

“…Results We obtained 41 full-length sequences of DENV from 80 PCR-positive samples, including 24 sequences (58.5%) from 2022 and 17 sequences (41.5%) from 2023. …”
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HBV sequence integrated to enhancer acting as oncogenic driver epigenetically promotes hepatocellular carcinoma development by Lu Chen, Wenxuan Li, Wenjing Zai, Xiangyi Zheng, Xianlong Meng, Qunyan Yao, Wei Li, Ying Liang, Mu Ye, Kaicheng Zhou, Mengxing Liu, Zhicong Yang, Zhanrui Mao, Hongyan Wei, Shuai Yang, Guoming Shi, Zhenghong Yuan, Wenqiang Yu

“…Animal models were also used to evaluate the therapeutic effect of HBV-miR-2 inhibitors. …”
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Mitochondrial DNA Sequence Variants Associated With Blood Pressure Among 2 Cohorts of Older Adults by Thomas W. Buford, Todd M. Manini, John A. Kairalla, Mary M. McDermott, Carlos A. Vaz Fragoso, Haiying Chen, Roger A. Fielding, Abby C. King, Anne B. Newman, Gregory J. Tranah

“…We examined the role of mitochondrial genomic variation in blood pressure by sequencing the mitochondrial genome. Methods and Results Mitochondrial DNA (mtDNA) data from 1755 participants from the LIFE (Lifestyle Interventions and Independence for Elders) studies and 788 participants from the Health ABC (Health, Aging, and Body Composition) study were evaluated using replication analysis followed by meta‐analysis. …”
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Improved resolution of avian influenza virus using Oxford Nanopore R10 sequencing chemistry by Jeremy D. Ratcliff, Brian Merritt, Hannah Gooden, Jurre Y. Siegers, Abhinaya Srikanth, Sokhoun Yann, Sonita Kol, Sarath Sin, Songha Tok, Erik A. Karlsson, Peter M. Thielen

“…In this study, human and avian influenza isolates (n = 45) from Cambodia were sequenced using both R9.4.1 and R10.4.1 flow cells and chemistries to evaluate performance between approaches. …”
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GNNSeq: A Sequence-Based Graph Neural Network for Predicting Protein–Ligand Binding Affinity by Somanath Dandibhotla, Madhav Samudrala, Arjun Kaneriya, Sivanesan Dakshanamurthy

“…<b>Methods:</b> GNNSeq predicts ligand binding affinity by extracting molecular characteristics and sequence patterns from protein and ligand sequences. …”
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An exploratory study on the application of nanopore sequencing for detecting Mycobacterium tuberculosis drug resistance in respiratory specimens by Niansa Wei, Feiyi Du, Wenjuan Nie, Yingxing Nong, Yanrong Lin, Aichun Huang, Shaoyong Xi, Yiyi Lan, Xiaoyang Luo, Shixiong Yang, Qingdong Zhu

“…Abstract Background This study aimed to evaluate the diagnostic efficacy of nanopore sequencing for Mycobacterium tuberculosis (MTB) drug resistance in respiratory specimens from pulmonary tuberculosis (PTB) patients. …”
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Whole-genome sequencing and biofilm gene characterization in multidrug-Resistant Staphylococcus aureus clinical strains by Emira Noumi, Tarek Zmantar, Nouha Bouali, Abdulrahman S. Bazaid, Khulood Fahad Alabbosh, Kamel Chaieb, Hisham N. Altayb, Vincenzo De Feo, Mejdi Snoussi

“…Biofilm production was evaluated using Congo-red agar plates (CRA), polystyrene microtiter plate technique (MtP), and adherence to human epithelial cells (Hep 2). …”
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Combining handcrafted and learned features using deep learning to improve protein-protein interaction prediction performance by Tran Hoai Nhan, Nguyen Phuc Xuan Quynh, Le Anh Phuong

“…The learned features are generated by Word2vec after being trained on a large database of protein sequences. Standard evaluation methods and benchmark datasets are used to assess the proposed model. …”
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