Does order matter? Investigating the effect of sequence on glance duration during on-road driving. by Joonbum Lee, Shannon C Roberts, Bryan Reimer, Bruce Mehler

“…Multiple statistical analyses were conducted to measure the effect of glance sequence on glance duration. Results showed that across both tasks and a variety of statistical tests, glance sequence had inconsistent effects on glance duration-the effects varied according to the number of glances, task type, and data set that was being evaluated. …”
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varVAMP: degenerate primer design for tiled full genome sequencing and qPCR by Jonas Fuchs, Johanna Kleine, Mathias Schemmerer, Julian Kreibich, Wolfgang Maier, Namuun Battur, Thomas Krannich, Somayyeh Sedaghatjoo, Lena Jaki, Anastasija Maks, Christina Boehm, Carina Wilhelm, Jessica Schulze, Christin Mache, Elischa Berger, Jessica Panajotov, Lisa Arnold, Björn Grüning, Markus Bauswein, Sindy Böttcher, Reimar Johne, Jürgen Wenzel, Martin Hölzer, Marcus Panning

“…We demonstrate the utility of varVAMP by designing and evaluating novel pan-specific primer schemes suitable for sequencing the genomes of SARS-CoV-2, Hepatitis E virus, rat Hepatitis E virus, Hepatitis A virus, Borna-disease-virus-1, and Poliovirus using clinical samples. …”
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Benchmarking metabolic RNA labeling techniques for high-throughput single-cell RNA sequencing by Xiaowen Zhang, Mingjian Peng, Jianghao Zhu, Xue Zhai, Chaoguang Wei, He Jiao, Zhichao Wu, Songqian Huang, Mingli Liu, Wenhao Li, Wenyi Yang, Kai Miao, Qiongqiong Xu, Liangbiao Chen, Peng Hu

“…Abstract Metabolic RNA labeling with high-throughput single-cell RNA sequencing (scRNA-seq) enables precise measurement of gene expression dynamics in complex biological processes, such as cell state transitions and embryogenesis. …”
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Simultaneous polyclonal antibody sequencing and epitope mapping by cryo electron microscopy and mass spectrometry by Douwe Schulte, Marta Šiborová, Lukas Käll, Joost Snijder

“…We evaluate the performance of the deep-learning tool ModelAngelo in determining de novo antibody sequences directly from reconstructed 3D volumes of antibody-antigen complexes. …”
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Sequence stratigraphic analysis for hydrocarbon prospectivity of the Kamta structure in the foredeep region of the Bengal Basin by Lima Akter Choiti, A.S.M. Woobaidullah, Shakhawat Hossain

“…This study applies sequence stratigraphic analysis to the Kamta and Rupganj gas fields in the Bengal Basin, aiming to refine the understanding of their stratigraphic architecture and evaluate their exploration potential. …”
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DNA sequence classification for diabetes mellitus using NuSVC and XGBoost: A comparative. by Said A Salloum, Khaled Mohammad Alomari, Ayham Salloum

“…With the advent of genomics, DNA sequence analysis has emerged as a promising approach to uncover the genetic markers associated with Diabetes Mellitus. …”
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Metagenomic next-generation sequencing of alveolar lavage fluid improves the detection of pulmonary infection by Meng Ziyu, Li Dong, Yang Wei, Tang Jihong

“…This study evaluated the effectiveness of metagenomic next-generation sequencing (mNGS) in detecting pathogens in patients with pulmonary infections, comparing a low-data-volume, human-depleted quantitative (Q) method and a high-data-volume, non-human-depleted pathogen capture engine (PACE) method. …”
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Comparison of Different HIV-1 Resistance Interpretation Tools for Next-Generation Sequencing in Italy by Daniele Armenia, Luca Carioti, Valeria Micheli, Isabella Bon, Tiziano Allice, Celestino Bonura, Bianca Bruzzone, Fiorenza Bracchitta, Francesco Cerutti, Giovanni Maurizio Giammanco, Federica Stefanelli, Maria Addolorata Bonifacio, Ada Bertoli, Marialinda Vatteroni, Gabriele Ibba, Federica Novazzi, Maria Rosaria Lipsi, Nunzia Cuomo, Ilaria Vicenti, Francesca Ceccherini-Silberstein, Barbara Rossetti, Antonia Bezenchek, Francesco Saladini, Maurizio Zazzi, Maria Mercedes Santoro

“…Background: Next-generation sequencing (NGS) is gradually replacing Sanger sequencing for HIV genotypic drug resistance testing (GRT). …”
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Characterization of hepatocellular adenoma and carcinoma using microRNA profiling and targeted gene sequencing. by Jian Zheng, Eran Sadot, Joana A Vigidal, David S Klimstra, Vinod P Balachandran, T Peter Kingham, Peter J Allen, Michael I D'Angelica, Ronald P DeMatteo, William R Jarnagin, Andrea Ventura

“…Tumor and non-tumor liver tissues were selected for immunohistochemical staining, small RNA sequencing, and targeted gene sequencing. We compared microRNA expressions and mutations between HCA and HCC and non-neoplastic liver.…”
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Use of four next-generation sequencing platforms to determine HIV-1 coreceptor tropism. by John Archer, Jan Weber, Kenneth Henry, Dane Winner, Richard Gibson, Lawrence Lee, Ellen Paxinos, Eric J Arts, David L Robertson, Larry Mimms, Miguel E Quiñones-Mateu

“…Although several next-generation sequencing (NGS) platforms are available, to date all published deep sequencing HIV-1 tropism studies have used the 454™ Life Sciences/Roche platform. …”
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Application of metagenomic next-generation sequencing in treatment guidance for deep neck space abscess by Han Lei, Jiarui Liao, Yu Lin, Tianrun Liu, Wenbin Lei, Wenxiang Gao

“…Effectiveness of mNGS-modified antimicrobial therapy was evaluated by comparing the treatment outcomes between two groups. …”
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Analysis of potential genes and pathways associated with the colorectal normal mucosa–adenoma–carcinoma sequence by Zhuoxuan Wu, Zhen Liu, Weiting Ge, Jiawei Shou, Liangkun You, Hongming Pan, Weidong Han

“…We discovered seven DEGs in the normal colorectal mucosa–adenoma–carcinoma sequence that was associated with CRC patient prognosis. …”
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Metagenomic Next-Generation Sequencing for the Diagnosis of Infectious Uveitis: A Comprehensive Systematic Review by Isabele Pardo, Luciana P. S. Finamor, Pedro S. Marra, Julia Messina G. Ferreira, Maria Celidonio Gutfreund, Mariana Kim Hsieh, Yimeng Li, João Renato Rebello Pinho, Luiz Vicente Rizzo, Takaaki Kobayashi, Daniel J. Diekema, Michael B. Edmond, Paulo J. M. Bispo, Alexandre R. Marra

“…This review evaluates its diagnostic performance in detecting infectious uveitis. …”
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Application of low-coverage whole-genome sequencing technology in risk stratification of colorectal adenomas by Guo Zhili, Xue Yuyue, Yu Fang, Ren Dianqun, Zhang Qin, Liu Jie, Liu Jie

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Refining the resolution of the yeast genotype–phenotype map using single-cell RNA-sequencing by Arnaud N'Guessan, Wen Yuan Tong, Hamed Heydari, Alex N Nguyen Ba

“…To overcome these challenges, we leveraged the cost efficiency and scalability of single-cell RNA sequencing (scRNA-seq) by collecting data from 18,233 yeast cells from 4489 F2 segregants derived from an F1 cross between the laboratory strain BY4741 and the vineyard strain RM11-1a. …”
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Clinical genome sequencing in patients with hereditary breast and ovarian cancer: Concept, implementation and benefits by Dennis Witt, Marc Sturm, Antje Stäbler, Benita Menden, Lisa Ruisinger, Kristin Bosse, Ines Gruber, Andreas Hartkopf, Silja Gauß, German Demidov, Nicolas Casadei, Elena Buena Atienza, Kira Mehnert, Janna Witt, Caspar Gross, Leon Schütz, Christopher Schroeder, Stephan Ossowski, Andreas Dufke, Tobias B. Haack, Olaf Riess, Ulrike Faust

“…This single-center real-world study aims to evaluate advantages of short-read genome sequencing (GS) in HBOC families. …”
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Exome sequencing of patients with syndromic tall stature reveals four novel candidate genes by Gabriela Jeesoo Kim, Edoarda Vasco de Albuquerque Albuquerque, Raissa C Rezende, Laurana De Polli Cellin, Ana Maria Santillan Vasconez, Ana C V Krepischi, Lucas Santana, Antônio Marcondes Lerario, Vinicius de Souza, Renata Scalco, Alexander A L Jorge

“…This study aimed to evaluate a cohort of patients with syndromic tall stature of unknown etiology via exome sequencing (ES) to identify novel candidate genes for overgrowth conditions. …”
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Identification of causative agents of infective endocarditis by metagenomic next-generation sequencing of resected valves by Vladimir Lazarevic, Nadia Gaïa, Truong-Thanh Pham, Mikaël de Lorenzi-Tognon, Mikaël de Lorenzi-Tognon, Myriam Girard, Florian Mauffrey, Yannick Charretier, Gesuele Renzi, Christoph Huber, Jacques Schrenzel, Jacques Schrenzel, Jacques Schrenzel

“…This study aimed to evaluate the effectiveness of metagenomic next-generation sequencing (mNGS) of cardiac valve specimens compared to conventional clinical laboratory methods for identifying pathogens in IE.MethodsNineteen patients with suspected IE who were scheduled for surgical valve removal were prospectively enrolled. …”
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Consistent microbial insights across sequencing methods in soil studies: the role of reference taxonomies by Niranjana Rose Edwin, Aoife Duff, Coline Deveautour, Fiona Brennan, Florence Abram, Orla O'Sullivan

“…To elucidate how these microbes relate to ecosystem functions, accurate identification and classification of soil microorganisms are important. We evaluated the comparability of shotgun and amplicon sequencing approaches by profiling soil microbiota from 131 diverse temperate grassland soils across Ireland. …”
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Assessment of traumatic mandibular nerve using MR neurography sequence: a preliminary study by Hyunwoo Yang, Nak-hoon Son, Dongwook Kim, Jae-Hee Chun, Jin Sung Kim, Tae Kyung Oh, Minwook Lee, Hyung Jun Kim

“…Conclusions An increase in FSNR and FNMCNR within our MRI sequence seems to be accurate indicators of the presence of traumatic nerve. …”
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