Limitations of 18S rDNA Sequence in Species-Level Classification of Dictyostelids by Thanyaporn Chittavichai, Sukhita Sathitnaitham, Supanut Utthiya, Wanasilp Prompichai, Kamonchat Prommarit, Supachai Vuttipongchaikij, Passorn Wonnapinij

“…Dictyostelid species classification has traditionally relied on morphology, a time-intensive method requiring expert knowledge. This study evaluated the potential and limitations of using the 18S rDNA sequence for species-level classification. 18S rDNA sequences of 16 samples from the Dicty stock center, including 14 samples found in Thailand, were analyzed. …”
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BioSeq-Diabolo: Biological sequence similarity analysis using Diabolo. by Hongliang Li, Bin Liu

“…BioSeq-Diabolo will integrate different biological sequence similarities in a supervised manner by using Learning to Rank (LTR), and the performance of the constructed methods will be evaluated and analysed so as to recommend the best methods for the users. …”
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TwinCons: Conservation score for uncovering deep sequence similarity and divergence. by Petar I Penev, Claudia Alvarez-Carreño, Eric Smith, Anton S Petrov, Loren Dean Williams

“…TwinCons detects conserved residues within regions of high functional importance for the ribosomal RNA (rRNA) and demonstrates that signatures are not confined to specific regions but are distributed across the rRNA structure. The ability to evaluate both nucleic acid and protein alignments allows TwinCons to be used in combined sequence and structural analysis of signatures and conservation in rRNA and in ribosomal proteins (rProteins). …”
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Phylogenetic analysis of mitochondrial D-loop sequence of Mongolian wild boars by Ali Khamit, Munkhjargal Bayarlkhagva, Davaa Bazarsad, Bolortuya Ulziibat, Bayarlkhagva Damdin, Bayarmaa Gun-Aajav

“…It is highly likely that the Mongolian subpopulation of wild boars, such as S. scrofa raddianus and S. scrofa nigripes, have had the same ancestor. In order to fully evaluate the distribution, ecology, and biology of Mongolian wild boars, it is essential to compare supplemental gene sequences that can reveal phylogenetic differences from the populations in the neighboring areas, such as Russia, northeast China, and Kazakhstan. …”
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Microhaplotype deep sequencing assays to capture Plasmodium vivax infection lineages by Mariana Kleinecke, Edwin Sutanto, Angela Rumaseb, Kian Soon Hoon, Hidayat Trimarsanto, Ashley Osborne, Paulo Manrique, Trent Peters, David Hawkes, Ernest Diez Benavente, Georgia Whitton, Sasha V. Siegel, Richard D. Pearson, Roberto Amato, Anjana Rai, Nguyen Thanh Thuy Nhien, Hoang Chau Nguyen, Ashenafi Assefa, Tamiru S. Degaga, Dagimawie Tadesse Abate, Awab Ghulam Rahim, Ayodhia Pitaloka Pasaribu, Inge Sutanto, Mohammad Shafiul Alam, Zuleima Pava, Tatiana Lopera-Mesa, Diego Echeverry, Tim William, Nicholas M. Anstey, Matthew J. Grigg, Nicholas P. Day, Nicholas J. White, Dominic P. Kwiatkowski, Aimee R. Taylor, Rintis Noviyanti, Daniel Neafsey, Ric N. Price, Sarah Auburn

“…We developed a high-throughput amplicon sequencing assay comprising 93 multi-SNP (microhaplotype) markers to determine IBD between P. vivax clinical isolates. …”
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Application of metagenomic next-generation sequencing in pathogen detection of lung infections by Song Chen, Song Chen, Tanglin Ouyang, Tanglin Ouyang, Kaiyang Wang, Xuan Hou, Xuan Hou, Rong Zhang, Rong Zhang, Meiyong Li, Haibin Zhang, Qinghua He, Xiuzhen Li, Zezhang Liu, Zezhang Liu, Xiaozhong Wang, Bo Huang

“…BackgroundMetagenomic next-generation sequencing (mNGS) has been widely reported to provide crucial information for the diagnosis and treatment of infectious diseases. …”
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Diagnostic Value of Metagenomic Next-Generation Sequencing for Pneumonia in Immunocompromised Patients by Jun Li, Chao-E. Zhou, Shan-Chen Wei, Li-Na Wang, Ming-Wei Shi, Chun-Ping Sun, Lian-Jun Lin, Xin-Min Liu

“…Metagenomic next-generation sequencing (mNGS) has emerged as a promising infection diagnostic technique. …”
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Multi-ancestry whole genome sequencing analysis of lean body mass by Xiaoyu Zhang, Kuan-Jui Su, Bodhisattwa Banerjee, Ittai Eres, Yi-Hsiang Hsu, Carolyn J. Crandall, Rajashekar Donaka, Zhe Han, Rebecca D. Jackson, Hanhan Liu, Zhe Luo, Braxton D. Mitchell, Chuan Qiu, Qing Tian, Hui Shen, Ming-Ju Tsai, Kerri L. Wiggins, Hanfei Xu, Michelle Yau, Lan-Juan Zhao, Xiao Zhang, May E. Montasser, Douglas P. Kiel, Hong-Wen Deng, Ching-Ti Liu, David Karasik

“…Using whole genome sequencing (WGS)-based studies, we aimed to discover novel genetic variants associated with LM in population-based cohorts with multiple ancestries. …”
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An Approach for Differential Diagnosis of Breast Tumors by ctDNA Methylation Sequencing by Xianyu Zhang, Yanling Yin, Zhujia Ye, Xingda Zhang, Wei Wei, Yi Hao, Liuhong Zeng, Ting Yang, Dalin Li, Jun Wang, Dezhi Zhao, Yanbo Chen, Shan Lei, Yongdong Jiang, Youxue Zhang, Shouping Xu, Abiyasi Nanding, Yajie Gong, Siwei Li, Yuanyuan Yu, Shilu Zhao, Siyu Liu, Yashuang Zhao, Zhiwei Chen, Shihui Yu, Jian‐Bing Fan, Da Pang

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Macrophage Changes and High‐Throughput Sequencing in Aging Mouse Intervertebral Disks by Wei Wang, Cheng Jiang, Jiong‐Hui Chen, Yong‐Long Chen, Zhen‐Wu Zhang, Zhi‐Chao Yang, Jun Li, Xiao‐Chuan Li

“…Four mice per group were selected for high‐throughput sequencing and 10 for tail IVD immunohistochemical analysis. …”
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Broad-range polymerase chain reaction and sequencing for the diagnosis of infectious diseases by Nicole E. Putnam, Drew W. Charles, James B. Doub, J. Kristie Johnson

“…Organisms identified by BR-PCR were compared to bacterial (n = 302), fungal (n = 137), and AFB (n = 111) cultures to determine concordance and were evaluated to determine if they led to a change in clinical management. …”
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Antifatigue Effect and Peptide Sequence of Oligopeptides Derived from Yellow Chicken by Miao LI, Jiefang FENG, Yurong MA, Xianjun ZENG, Penglei CUI, Xuefei YE, Liang CHEN, Yuxin YANG, Ting LIU, Lei FANG

“…Lactate dehydrogenase (LD), creatine kinase (CK) levels, and muscle/liver glycogen content changes in the mice were measured to evaluate their antifatigue effects in vivo after continuous gavage of mice for 28 days. …”
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The presence of multiple variants of IncF plasmid alleles in a single genome sequence can hinder accurate replicon sequence typing using in silico pMLST tools by Michaela Ruzickova, Jana Palkovicova, Ivo Papousek, Max L. Cummins, Steven P. Djordjevic, Monika Dolejska

“…The classification of IncF plasmids using the plasmid Multi-Locus Sequence Typing (pMLST) tool from the Center for Genomic Epidemiology (CGE; https://www.genomicepidemiology.org/) compares the sequences of IncF alleles against a database to create a plasmid sequence type (ST). …”
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A Semi-Automatic Framework for Practical Transcription of Foreign Person Names in Lithuanian by Gailius Raškinis, Darius Amilevičius, Danguolė Kalinauskaitė, Artūras Mickus, Daiva Vitkutė-Adžgauskienė, Antanas Čenys, Tomas Krilavičius

“…Focusing on noisy, web-crawled data, the pipeline combines rule-based filtering, morphological normalization, and manual stress annotation—the only non-automated step—to generate training data for character-level transcription models. We evaluate three approaches: a weighted finite-state transducer (WFST), an LSTM-based sequence-to-sequence model with attention, and a Transformer model optimized for character transduction. …”
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A lightweight neural attention-based model for service chatbots by Sinarwati Mohamad Suhaili, Mohamad Nazim Jambli

“…To validate its effectiveness, the proposed model was evaluated on a real-world Customer Support Twitter dataset. …”
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3D-FSE-XETA-T1WI平扫序列在评估MCA狭窄中的诊断价值：VWI多序列与DSA的对照研究(The Diagnostic Value of 3D-FSE-XETA-T1WI Plain Scan Sequence in Evaluating MCA Stenosis: A Control Study of VWI Multi-Sequence and DSA) by 尹雪梅，赵晓慧，李文菲，吴磊，刘兰祥，张俊(YIN Xuemei, ZHAO Xiaohui, LI Wenfei, WU Lei, LIU Lanxiang, ZHANG Jun)

“…Conclusions The 3D-FSE-XETA-T1WI plain scan sequence in VWI is superior to other sequences in evaluating the stenosis rate of the MCA-M1 segment and can serve as an effective method to assist DSA in evaluating the stenosis severity before clinical surgery.…”
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Evaluation of the diagnostic concordance of FDA-approved PD-L1 assays in clear cell renal cell carcinoma by Yoko Koh, Taigo Kato, Gaku Yamamichi, Eisuke Tomiyama, Makoto Matsushita, Koji Hatano, Atsunari Kawashima, Norio Nonomura, Motohide Uemura

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Evaluation Revolutions by Michael Scriven

“…More precisely, it developed into a family of sub-disciplines—product evaluation, program evaluation, personnel evaluation, policy analysis, etc. …”
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Apprentice bot model design and implementation for psychological clients’ therapy by Kibrom Gidey, Hailay Beyene, Fiseha Haileslassie, Haben Berihu

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Investigating Attributes of Oil Source Rocks by Combining Geochemical Approaches and Basin Modelling (Central Gulf of Suez, Egypt) by Moataz Barakat, Mohamed Reda, Dimitra E. Gamvroula, Robert Ondrak, Dimitrios E. Alexakis

Subjects: “…Upper Senonian to Middle Miocene sequence…”
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