Evaluation of a national digital pre-implantation biopsy service for deceased-donor kidney transplantation in the UK (Pithia trial); a stepped-wedge cluster randomised registry tri... by Dominic M. Summers, John O.O. Ayorinde, Desley A. Neil, Karla Hemming, Laura Smith, Jennifer Mehew, Helen Thomas, Rosie Brown, Suzie Phillips, Emma Laing, Anna Sidders, Alison J. Deary, Rachel J. Johnson, Victoria Bardsley, Sathia Thiru, Meryl H. Griffiths, Ranmith Perera, Owen Cain, Candice Roufosse, Naomi Simmonds, Michael Sheaff, Natalie Brearley, Bindu Vydianath, Anna Paterson, Kishore Gopalakrishnan, Anastasios Chatzitolios, Chris J. Callaghan, Richard Jarvis, Frank J.M.F. Dor, Smarajit Dutta, Atul Bagul, Colin Wilson, Dan Ridgway, Adam Barlow, Richard Baker, Adnan Sharif, James McDaid, John Terrace, Lorna Marson, Debabrata Roy, James Hunter, Laszlo Szabo, Simon Knight, Karen Stevenson, Patrick Mark, Samuel Turner, Zia Moinuddin, Abbas Ghazanfar, Rajesh Sivaprakasam, Reza Motallebzadeh, Nicholas Torpey, Gareth Jones, Catherine Boffa, Paul Gibbs, Andrew Connor, Imran Saif, Badri Shrestha, Harry Hill, James Fotheringham, Gavin J. Pettigrew

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Emergence and detection of gastro‐enteric virulence genes in non‐pathogenic Vibrio cholerae: Implications for public health and water safety in African developmental regions by Bright Esegbuyota Igere

“…It also suggests a current potential shift in the virulence dynamics of V. cholerae strains recovered from the study area and a re‐evaluated view of the previously non‐pathogenic V. cholerae strains. …”
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Evaluation of the BD GeneOhm MRSA and VanR Assays as a Rapid Screening Tool for Detection of Methicillin-Resistant Staphylococcus aureus and Vancomycin-Resistant Enterococci in a T... by H. Hassan, M. Shorman

“…The aim of this study was to evaluate the diagnostic performance of BD GeneOhm VanR Assay, a rapid PCR test that detects the presence of vanA and/or vanB genes and the performance of BDGeneOhm MRSA Assay which detects the staphylococcal cassette chromosomemec (SCCmec cassette) carrying the mecA gene and Staphylococcus aureus specific sequence located within the orfX gene. …”
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A comparative study evaluating three line immunoassays available for serodiagnosis of equine Lyme borreliosis: Detection of Borrelia burgdorferi sensu lato-specific antibodies in s... by Cornelia V Broeckl, Stephanie Hiereth, Reinhard K Straubinger

“…Operability of the three LIAs was equally user-friendly with minor variations. In two LIAs, test-evaluation was simplified by a supplied scanner and evaluation software. …”
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A comparative study of manifold learning methods for scRNA-seq with a trajectory-aware metric by Mehdi Nadjafikhah, Mohammad Nasiri

“…Abstract Single-cell RNA sequencing (scRNA-seq) enables detailed analysis of cellular diversity, but the data’s high dimensionality presents analytical challenges. …”
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A hybrid 1DCNN-GRU deep learning framework for classifying caprine granulosa cell fertility potential using single-cell transcriptomics by Thanida Sananmuang, Denis Puthier, Kaj Chokeshaiusaha

“…Model performance was evaluated using accuracy, precision, recall, and F1 score. …”
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Preoperative plasma cell-free DNA chromosomal instability predicts microvascular invasion in hepatocellular carcinoma: a prospective study by Zheyue Shu, Ting Ye, Wei Wu, Menghan Su, Jingcheng Wang, Min Zhang, Ziliang Qian, Haifen Huang, Shusen Zheng, Qi Xia

“…Receiver operating characteristic (ROC) curve was used to evaluate the performance of these parameters in preoperative MVI prediction. …”
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Integrated Detection of Respiration and Heartbeat With Communication Capabilities Using OFDM-LFM-MP Signals by Zhaoshen Dong, Yan Liu, Yukai Ouyang

“…Initially, we developed an OFDM-LFM-MP signal by utilizing M-sequences with strong auto-correlation properties for coding communication data. …”
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How does interactive case-based learning improve students' complex mathematical problem-solving abilities? by Ramdani Miftah, Jarnawi Afghani Dahlan, Lia Kurniawati, Tatang Herman, Lutfiana Lutfiana

“…The participants were seventh-grade students studying sequences and series. The research utilized ICBL-based teaching materials, CMPS ability tests, and student response questionnaires. …”
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From concept to approval: human genomic data integration with population observational data – insights from a Belgian pilot study by Nina Van Goethem, Johan Van der Heyden, Pierre Smith, Wannes Van Hoof, Harlinde De Schutter, Melissa Van Bossuyt, Karin De Ridder, Marc Van Den Bulcke, Emilie Cauët

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Efficacy of liquid biopsy for genetic mutations determination in non-small cell lung cancer: a systematic review on literatures by Mohammad Mehdi Jahani, Parisa Mashayekhi, Mir Davood Omrani, Azita Azimi Meibody

“…This systematic review aims to evaluate the efficacy of liquid biopsy in detecting genetic mutations in NSCLC, focusing on its sensitivity, specificity, clinical utility, and potential to guide personalized treatment strategies. …”
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Time-coexpress: temporal trajectory modeling of dynamic gene co-expression patterns using single-cell transcriptomics data by Shuyi Yang, Anderson Bussing, Giampiero Marra, Michelle L. Brinkmeier, Sally A. Camper, Shannon W. Davis, Yen-Yi Ho

“…Abstract Background The rapid advancement of single-cell RNA sequencing (scRNAseq) technology provides high-resolution views of transcriptomic activity within individual cells. …”
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Standardization and validation of a novel reverse transcriptase polymerase chain reaction method for detecting virulent strains of the infectious bursal disease virus by Vladimir Longa-Bobadilla, Phillip Ormeño-Vásquez, Manuel Criollo-Orozco, Luis Tataje-Lavanda, Katherine Huamán-Gutierrez, Ángela Montalván, Mirko Zimic, Manolo Fernández-Sanchez, Manolo Fernández-Díaz

“…Materials and Methods: Eight VP5 sequences were aligned, and the sequence with the majority of nucleotide coincidences was used to design a set of consensus primers. …”
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Characterization and prognostic of CD8 + TIM3 + CD101 + T cells in glioblastoma multiforme by Hong-Liang Wang, Sai Li, Chun-Chun Ma, Xiang-Hu Zheng, Hao-Yuan Wu, Chen-Xi Chang, Zhi-Hao Yang, Jia-Wei Wang, Fa-Ming Pan, Bing Zhao

“…Methods Analysis of data from TCGA, CGGA, and GEO databases included whole-genome expression profiles, clinical data, single nucleotide mutations, and single-cell RNA sequencing. DEGs were identified, and cell trajectories were constructed using Seurat, Monocle 2, and CellChat packages. …”
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Detection and In Vivo Validation of <i>Dichorhavirus</i> e-Probes in Meta-Transcriptomic Data via Microbe Finder (MiFi^®) Discovers a Novel Host and a Possible New Strain... by Avijit Roy, Jonathan Shao, Andres S. Espindola, Daniel Ramos Lopez, Gabriel Otero-Colina, Yazmín Rivera, Vessela A. Mavrodieva, Mark K. Nakhla, William L. Schneider, Kitty Cardwell

“…The E-probe Diagnostic for Nucleic Acid Analysis (EDNA) was developed to reduce computational effort and then integrated within Microbe-Finder (MiFi^®) online platform to design and evaluate e-probes in raw High Throughput Sequencing (HTS) data. …”
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Comparative analysis of oral microbiome in saliva samples of oral leukoplakia, proliferative leukoplakia and oral squamous cell carcinoma by Rossella Intini, Sol Balsells, Leticia Bagan, Giulio Fortuna, Herve Sroussi, Jose Bagan, Jose Bagan

“…The V3–V4 regions of the 16S rRNA gene were sequenced using the Illumina MiSeq platform. Bioinformatic analyses, including diversity assessments and taxonomic classification with the SILVA v138 database, were performed using QIIME2. …”
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Towards Meaningful Interpretation of Molecular Data: Insights Gained from HMMD Challenges in <i>Salmonella</i> Detection for Future NGS Integration in Clinical Microbiology by Hyunji Kim, Soo Hyun Seo, Jae-Seok Kim, Kwang Jun Lee, Kyoung Un Park

“…<b>Background:</b> With advancements in molecular diagnostics, including Highly Multiplexed Microbiological/Medical Countermeasure Diagnostic Devices (HMMDs) and the impending integration of Next-Generation Sequencing (NGS) into clinical microbiology, interpreting the flood of nucleic acid data in a clinically meaningful way has become a crucial challenge. …”
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Single cell transcriptomic analysis reveals tumor immune infiltration by macrophage cells gene signature in lung adenocarcinoma by Xiaotong Guo, Youjun Deng, Wenjun Jiang, Heng Li, Yisheng Luo, Huachuan Zhang, Hao Wu

“…We aimed to identify novel TAM-related biomarkers and significance of macrophage infiltration in lung adenocarcinoma (LUAD) through an integrative analysis of single-cell RNA-sequencing (scRNA-seq) data. To describe the cell atlas and construct a novel prognostic signature in LUAD. …”
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Transcriptomic Analysis of Menstrual Blood-Derived Stem Cells Transplantation Combined with Exercise Training in Promoting Spinal Cord Injury Recovery in Rats by QI Longju, CHEN Shiyuan, LIAO Zehua, SHI Yuanhu, SUN Yuyu, WANG Qinghua

“…Spinal cord tissue from the injury site was selected for transcriptome sequencing, and mRNA expression data from both the SCI and CTMT groups were analyzed. …”
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Cell types and neuronal genetic architecture in the rat CSF-contacting nucleus and the role of 5-HT in this nucleus in mediating morphine addiction through the brain–CSF circuit by Ying Li, Ying Li, Ying Li, Bin Gui, Bin Gui, Bin Gui, Yijun Zhang, Yijun Zhang, Yijun Zhang, Licai Zhang, Licai Zhang, Licai Zhang

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