Analysis of somatic mutations in the <i>JAK2</i>, <i>CALR</i>, <i>MPL</i> and <i>ASXL1</i> genes and evaluation of their impact on the survival of patients with myelofibrosis by T. N. Subbotina, I. E. Maslyukova, K. S. Semashchenko, G. A. Khodos, D. V. Kurochkin, A. A. Shalyova, M. A. Mikhalev, E. V. Vasiliev, M. G. Osadchaya, E. A. Dunaeva, A. S. Esman, K. O. Mironov

“…To analyze driver (JAK2, CALR, MPL) and prognostic (ASXL1) somatic mutations in patients with MF and evaluate their impact on survival.Materials and methods. …”
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Research Progress on Ambient Visual Information Analysis and Perception Measurement Methods for Motion Vision by Haipeng ZHU, Yuhang KONG, (JPN) OHNO Ryuzo

“…These include real-time emotion evaluation using wearable sensors, physiological measurements, and machine learning models for multimodal data analysis. …”
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<i>Enterobacter soli Strain AF-22b-4245</i>: Study of the Genome and the Effect on Wheat Growth by Ekaterina Alexeevna Sokolova, Olga Viktorovna Mishukova, Inna Viktorovna Khlistun, Irina Nikolaevna Tromenschleger, Evgeniya Vladimirovna Chumanova, Elena Nikolaevna Voronina

“…A gene table was formed that allows us to evaluate the potential PGP properties of a microorganism based on the results of genome-wide sequencing. …”
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Identification of CWH43 as a novel prognostic biomarker and therapeutic target in clear cell renal cell carcinoma by a multi-omics approach and correlation with autophagy progressi... by Ailian Wu, Peng Bai, Hui Qu, Tao Zhang

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Associations Between Indoor Fungal Community Structures and Environmental Factors: Insights from the Evidence-Driven Indoor Air-Quality Improvement Study by Iva Šunić, Dubravka Havaš Auguštin, Jelena Šarac, Kristina Michl, Tomislav Cernava, Rasmus Riemer Jakobsen, Armin Mešić, Natalija Novokmet, Mario Lovrić

“…Dust samples from 66 Croatian households were analyzed using fungal ITS sequencing. Statistical analyses, including alpha diversity measures, were conducted to evaluate the influence of variables such as pet ownership, number of siblings, and cleaning habits on fungal diversity and abundance. …”
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Interlaboratory assessment of candidate reference materials for lentiviral vector copy number and integration site measurements by Hua-Jun He, Zhiyong He, Steven P. Lund, Laure Turner, Yongjun Fan, Yu Qiu, David C. Corney, Boro Dropulic, Rimas Orentas, Oxana Slessareva, Priscilla Welch, Katie Dungca, Ellen Stelloo, Gabrielle Dijksteel, Harma Feitsma, Sana Ahmed-Seghir, Rostyslav Makarenko, Engin Altunlu, Daniëlle Steenmans, Jan Spanholtz, Monica Raimo, Shai Senderovich, Barbara S. Paugh, Chieh-Yuan Li, Benjamin Schroeder, Alexandra S. Whale, Dilek Yener, Carole A. Foy, Shareef Nahas, Feng Tu, Michael Sheldon, Yan Ding, Jennifer Kandell, Uma Lakshmipathy, Jennifer H. McDaniel, Justin M. Zook, Sierra Miller, Samantha Maragh, Simona Patange, Mahir Mohiuddin, Alessandro Tona, Kenneth D. Cole, Sheng Lin-Gibson

“…The fixed clonal VCN cells were also used to evaluate an emerging imaging-based technology called molecular combing. …”
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Spastic Paraplegia Type 78 Associated With ATP13A2 Gene Variants in Compound Heterozygosity by R. Bermejo Ramírez, N. Villena Gascó, L. Ruiz Palmero, G. A. Ribes Bueno, E. Seiti Yamanaka, J. D. Arroyo Andújar

“…Methods A pathogenic variant screening in a Spanish HSP patient was carried out by whole‐exome sequencing, followed by a software filtering process and validation of candidate variants by Sanger sequencing. …”
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Hsa-miR-21-5p is induced by interleukin-6 and affects multiple pathogenic factors associated with fibroblast-like synoviocytes in rheumatoid arthritis by Kei Araki, Sho Mokuda, Hiroki Kohno, Naoya Oka, Hirofumi Watanabe, Michinori Ishitoku, Tomohiro Sugimoto, Yusuke Yoshida, Junya Masumoto, Shintaro Hirata

“…Herein, we investigated the relationship between miRNAs and FLS. Next-generation sequencing (small RNA-seq) was performed on primary cultured FLS derived from patients with RA to analyze the mature miRNA expression pattern. …”
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Targeting immune cellular populations and transcription factors: unraveling the therapeutic potential of JQF for NAFLD by Lijuan Zhou, Jingyi Zhao, Kaile Ma, Rui Hao, Chensi Yao, Xiaowen Gou, Chuanxi Tian, Li Wan, Min Li, Xiaolin Tong

“…However, its underlying mechanism of action remains unclear.MethodsUsing a high-fat diet-induced NAFLD mouse model, we evaluated JQF’s effects with biochemical tests and histopathology. …”
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BAX-mediated ammonia-driven cell death: a novel prognostic and therapeutic target in clear cell renal cell carcinoma by Xi Zhang, Zijie Yu, Lu Yin, Qiang Li, Shaohua He, Heng Li, Jian Li, Lian Sheng, Hongfei Wu, Hongqi Chen, Xiaoxu Zhu, Yang Lv

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Glomalin-Related Soil Proteins as Indicator of Soil Quality in Pig-Fertigated and Rainfed Systems by Josiquele G. Miranda, Eduardo G. Couto, Oscarlina L. S. Weber, Gilmar N. Torres, James M. Moura, Ricardo T. Tanaka, Marcos A. Soares

“…We assessed irrigated and rainfed systems by integrating the soil quality index (SQI) with total and easily extractable glomalin (T-GRSP, EE-GRSP), determining microbial diversity via eDNA amplicon sequencing, and evaluating enzyme activities across three soil depths (0–10, 10–20, and 20–30 cm). …”
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Viroscope™: a universal solution for plant virus and viroid diagnostics using HTS and cloud-based analysis by Verónica Morgante, Juan Cristóbal Jiménez, Claudio Ponce, Cristóbal Urrutia, Fernanda Vera, Camila Farías, Rocío Camps de la Maza, Valentina Caro, Marco Muñoz, Bernardo Pollak

“…While high-throughput sequencing (HTS) -based virus diagnosis offers promising applications, challenges in data analysis and implementation have limited widespread adoption. …”
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Case Report: A clinically relevant isolation of Gardnerella leopoldii guided by morphological and molecular evidence from a urinary tract infection case by Liangyou Chen, Liangyou Chen, Wei Weng, Dan Li, Weipeng Xie, Lingling Lu, Shuo Li, Shuo Li

“…To identify the causative pathogens, we employed a comprehensive approach that included microscopic morphology, Sanger sequencing, and metagenomic next-generation sequencing (mNGS). …”
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Molecular Epidemiology and Genetic Context of <i>optrA</i>-Carrying Linezolid-Resistant Enterococci from Humans and Animals in South Korea by Younggwon On, Sung Young Lee, Jung Sik Yoo, Jung Wook Kim

“…<i>optrA</i>-positive linezolid-resistant isolates were less frequent, with rates of 0.1%, 1.4%, 0.9%, and 1.0%, respectively. Multilocus sequence typing identified sequence types (STs) 330 and ST476 in <i>E. faecalis</i> from humans, with no shared STs between human and livestock isolates. …”
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Phenotypic characterization and complete genome of a tumorigenic pathobiont Escherichia coli LI60C3 by Linda Chia-Hui Yu, Shu-Chen Wei, Yi-Hsuan Li, Chung-Yen Huang, Yu-Chen Pai, Yuan-Mao Hung, Liang-Chuan Lai, Yen-Hsuan Ni

“…The objective is to characterize the tumorigenic E. coli through whole-genome sequencing (WGS) and phenotypic assays, and validate their presence in human CRC. …”
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The novel diagnostic markers for systemic lupus erythematosus and periodontal disease by Xuedi Cheng, Jinfeng Zhang, Jiali Hou, Xiaocui Han, Bin Han, Jun Zhou, Zhongjun Wang, Junzheng Wang

“…CIBERSORT and Gene Set Variation Analysis (GSVA) were used to evaluate the correlation between shared hub genes and immune infiltration patterns as well as metabolic pathways. …”
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Evidence of Mycobacterium bovis DNA in shared water sources at livestock–wildlife–human interfaces in KwaZulu-Natal, South Africa by Megan C. Matthews, Deborah M. Cooke, Tanya J. Kerr, Andre G. Loxton, Robin M. Warren, Giovanni Ghielmetti, Giovanni Ghielmetti, Elizabeth M. Streicher, Carmel S. Witte, Michele A. Miller, Wynand J. Goosen, Wynand J. Goosen

“…Water samples from 63 sites were screened for MTBC DNA by extracting DNA and performing hsp65 PCR amplification, followed by Sanger amplicon sequencing (SAS). Sequences were compared to the National Centre for Biotechnology Information (NCBI) database for genus or species-level identification. …”
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Development of a strain-specific PCR as a diagnostic tool for surveillance, detection, and monitoring of vancomycin-resistant Enterococcus faecium during outbreak by Artur J. Sabat, Lilli Gard, Monika A. Fliss, Viktoria Akkerboom, Robin F. J. Benus, Mariette Lokate, Andreas Voss, Erik Bathoorn

“…Multi locus sequence types (MLST-ST) and core genome sequence type clusters (cgMLST-CT) were determined with Ridom SeqSphere + software. …”
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Evaluation of medical student performance and satisfaction of simulator-based direct ophthalmoscopy training using a new refractive adjustment fundoscopic examination simulator—ICE... by Ratima Chokchaitanasin, Sritatath Vongkulsiri, Raveewan Choontanom

“…Training Sequence 2 started with the ICEyeModel, followed by a traditional simulator. …”
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A phase I, randomized, double-blind, parallel, single-dose pharmacokinetic study to evaluate the biosimilarity of KM118 (proposed pertuzumab biosimilar) with reference pertuzumab (... by Yan Li, Yu Wang, Caixia Yang, Na Zhao, Xinghe Wang

“…Similar immunogenicity was observed between Sequences A and B. All of the adverse events (AEs) were Grade 1 or 2, and no SAEs occurred. …”
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