Comprehensive analysis of plasma cell heterogeneity and immune interactions in multiple myeloma by Shuang Qu, Zhihai Zheng, Xiaoling Guo, Jiaqi Mei, Sicong Jiang, Biyun Chen

“…Through single-cell sequencing, 10 major cell types were identified and annotated. …”
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Construction of a macrophage-related prognostic signature and assessment of immune checkpoint inhibitor efficacy of HCC by Shouge Zang, Hongwei Chen, Yuqian Han, Di Cui, Jiangtao Yu

“…PLAUR-related cell communication was evaluated using CellChat. Functional assays were conducted to assess the effect of PLAUR knockdown on macrophage polarization, tumor cell behavior, and PI3K/AKT/mTOR pathway activity. …”
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Diagnosis of congenital ectopia lentis: a case report and review of the literature by Xuebi Cai, Wei Chen, Jiezheng Xue, Junliang Wang, Zhisha Bai, Wei Qiang, Sifang Zhang, Heding Zhou

“…Additionally, whole exome sequencing (WES) was performed to identify gene mutations potentially linked to the clinical manifestations. …”
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Do Colorectal Serrated and Non-Serrated Adenocarcinomas Differ in Somatic Mutations and Clinicopathologic Features? by Zeynep Sagnak Yilmaz, Sibel Demir Kececi, Sevdegul Aydin Mungan, Ismail Saygin, Ozgul Sagol, Sulen Sarioglu

“…A chi-square test was used to evaluate histopathologic characteristics and somatic mutations in SACs and non-serrated adenocarcinomas (non-SACs). …”
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Longitudinal dynamics of intestinal bacteria in the life cycle and their effects on growth and development of potato tuber moth by Qiaosi Fu, Wenqian Wang, Bin Chen, Yuxi Hu, Rui Ma, Enran Zhu, Sitong Jin, Haosheng Cai, Guanli Xiao, Guangzu Du

“…In this study, the dynamics of the intestinal bacterial community across the whole life cycle of PTM were evaluated using single molecule real-time sequencing. …”
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Brain injury in premature infants may be related to abnormal colonization of early gut microbiome by Li Hou, Jing Zhao, Linlin Yin, Lu Dai, Hong Deng, Lin Jiang

“…Methods Fecal samples from typical premature infants (non-brain injury group, n = 17) and those with brain injuries (brain injury group, n = 21) were collected on days 1, 3, and 7 after birth for 16 S rRNA sequencing. Alpha diversity analysis was used to evaluate the diversity of gut microbiome. …”
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Distinct germ-line genetic mutation patterns correlate with reproductive outcomes in ICSI patients: a pilot study by Ting Jiang, Yan Wang, Wandai Wu, Qianru Yang, Sixian Wu, Xueguang Zhang, Wenming Xu

“…Whole-exome sequencing identified distinct mutation patterns between groups. …”
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Foliar Application of <i>Bacillus thuringiensis</i> Enhances Tea Quality and Plant Defense via Phyllosphere Microbiome Modulation by Yulin Xiong, He Liu, Dongliang Li, Wei Xie, Zhong Wang, Xiaohong Fang, Jizhou Wang, Wei Chen, Xi Du, Yanyan Li, Chuanpeng Nie, Chuanhua Yin, Pumo Cai, Yongcong Hong

“…However, the effects of Bt spraying on tea quality and the structure and function of the phyllosphere microbiome remain unclear. This study evaluated the effects of Bt spraying on tea quality, microbiome composition, diversity, and potential functions using tea leaf quality measurements and high-throughput sequencing of the 16S/ITS rDNA genes. …”
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Gut metagenomic characteristics of ADHD reveal low Bacteroides ovatus-associated host cognitive impairment by Yan Li, Haiting Sun, Yufen Huang, Anqi Yin, Linjuan Zhang, Jiao Han, Yixuan Lyu, Xiangzhao Xu, Yifang Zhai, Huan Sun, Ping Wang, Jinyang Zhao, Silong Sun, Hailong Dong, Feng Zhu, Qiang Wang, Luis Augusto Rohde, Xuefeng Xie, Xin Sun, Lize Xiong

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Exploring the appropriate situation of performing CSF mNGS in patients with proposed intracranial infections by Jinliang Deng, Xiuxiao Chen, Yi Bu, Jinru Zhang, Jingzhe Han

“…Abstract Background Identifying the responsible pathogen is crucial for precision medicine in intracranial infections, and Cerebrospinal Fluid (CSF) Metagenomic Next-Generation Sequencing (mNGS) is a reliable method for this detection. …”
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Microbial diversity of soils under different land use and chemical conditions by Jung-Hwan Yoon, Mahesh Adhikari, Seok Soon Jeong, Sang Phil Lee, Hyuck Soo Kim, Geon Seung Lee, Duck Hwan Park, Heejung Kim, Jae E. Yang

“…Alpha diversity, assessed using Chao1 and Shannon indices, and beta diversity, evaluated through Bray-Curtis dissimilarity and Principal Coordinates Analysis (PCoA), were used to characterize microbial diversity. …”
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The microbiome analysis of ripen grape berries supports the complex etiology of sour rot by Chiara Brischetto, Chiara Brischetto, Vittorio Rossi, Vittorio Rossi, Giorgia Fedele, Giorgia Fedele

“…The 16S rRNA gene, ITS high-throughput amplicon sequencing, and quantitative PCR were used to assess the relative abundance and dynamic changes of microorganisms associated with SR. …”
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Carotid Endarterectomy Ameliorates Cognitive Impairment in Clinical and Experimental Unilateral Carotid Artery Stenosis by Yijun Cheng, Bin Chen, Miao Zhang, Zhenghong Chen, Mingjian Liu, Ziqian Zhang, Hao Tang, Dapeng Wang, Wenwen Lv, Biao Li, Yuting Dai, Hanbing Shang

“…Genomic sequencing of the white matter was performed to explore the potential underlying mechanism. …”
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Genome-wide identification and association analysis of informative SNPs of various nutri-nutraceutical traits in Buckwheat (Fagopyrum spp.) by Madhiya Manzoor, Jebi Sudan, Adil Nath, Basharat Bhat, Parvaze A. Sofi, M. Ashraf Bhat, P. V. Vara Prasad, Sajad Majeed Zargar

“…Using 132 diverse genotypes, we evaluated 10 key nutritional and nutraceutical traits: phenol, flavonoids, antioxidants, methionine, lysine, protein content, nitrogen, iron, zinc, and ascorbic acid. …”
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Immune microenvironment spatial landscapes of tertiary lymphoid structures in gastric cancer by Yi Xie, Haoxin Peng, Yajie Hu, Keren Jia, Jiajia Yuan, Dan Liu, Yanyan Li, Xujiao Feng, Jian Li, Xiaotian Zhang, Yu Sun, Lin Shen, Yang Chen

“…Multispectral fluorescence imaging was employed to evaluate variations in immune cell infiltration across different TLS sites among 110 GC patients, by quantifying immune cell density and spatial characteristics. …”
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Immunohistochemical and molecular evolutionary features of jejunoileal adenocarcinoma unveiled through comparative analysis with colorectal adenocarcinoma by Rei Ishikawa, Hidetaka Yamada, Hirotomo Saitsu, Ryosuke Miyazaki, Juri Takahashi, Rino Takinami, Satoshi Baba, Mitsuko Nakashima, Moriya Iwaizumi, Satoshi Osawa, Hideya Kawasaki, Yoshifumi Arai, Yoshiro Otsuki, Hiroshi Ogawa, Hiroki Mori, Fumihiko Tanioka, Shioto Suzuki, Kazuyo Yasuda, Makoto Suzuki, Haruhiko Sugimura, Kazuya Shinmura

“…To better understand these differences, we selected three dMMR-JIACs and eight mismatch repair proficient (pMMR)-JIACs and evaluated molecular evolutionary history by multi-regional whole-exome sequencing. …”
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Single-cell and bulk RNA analysis identifies EMT-associated CAF signatures and prognostic model in lung adenocarcinoma by He An, Ping An

“…Methods We integrated RNA sequencing data and clinical information of LUAD patients from the TCGA and GEO databases, along with single-cell RNA sequencing data for cell subtype identification and EMT activity analysis. …”
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Isolation, Identification, Whole-genome Characterization, and Comparative Genomic Analysis of Pathogenic Aeromonas hydrophila A01 from Pelteobagrus fulvidraco by Wei GAO, Ling SHAO

“…Strain affinities were evaluated through ANI analysis and phylogenetic tree construction based on homologous single-copy genes, complemented by multilocus sequence typing (MLST) to discern genetic backgrounds.…”
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Molecular Characterization of Multidrug-Resistant and Hypervirulent New Delhi Metallo-Beta-Lactamase <i>Klebsiella pneumoniae</i> in Lazio, Italy: A Five-Year Retrospective Study by Claudia Rotondo, Carolina Venditti, Ornella Butera, Valentina Dimartino, Francesco Messina, Michele Properzi, Claudia Caparrelli, Valentina Antonelli, Silvia D’Arezzo, Marina Selleri, Carla Nisii, Carla Fontana, on behalf of the Lazio Region Laboratory Study Group

“…In silico analysis of acquired resistance and virulence genes was performed using whole-genome sequencing (WGS), molecular typing through MLST, and core genome multi-locus sequence typing (cgMLST). …”
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Effect of Wheat Varieties and Cultivation Environments on Grain Endophytes, Microbial Communities, and Quality of Medium-High Temperature Daqu in Chinese Baijiu by Huixian Zhou, Mengmeng Zhao, Qinqin Xiong, Chengcheng Feng, Zhien Pu, Guoyue Chen, Songtao Wang, Yi Dong, Xiaojun Wang, Hai Long, Qiantao Jiang, Jirui Wang, Yuming Wei, Youliang Zheng, Wei Li

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