Changes of respiratory microbiota associated with prognosis in pulmonary infection patients with invasive mechanical ventilation-supported respiratory failure by Yong Sun, Kai Guo, Jing Tang, Junjie Zhao, Xiaojing Zhang, Youqin Yan, Lingmin Yuan, Yi Zhang, Canhu Qiu, Jian Luo, Weiwen Zhang, Honglong Fang, Juan Chen

“…This study aimed to evaluate the clinical performance of metagenomic next-generation sequencing (mNGS) testing in pathogen diagnosis, medication guidance and to explore dynamic changes in the respiratory microbiota associated with prognosis.Methods This multicenter retrospective study enrolled ICU patients from five hospitals who underwent invasive mechanical ventilation (IMV) and had pathogenic microorganisms identified by both mNGS and conventional microbiological tests (CMT) from December 2021 to April 2024. …”
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Pathogen detection and antibiotic use in granulomatous lobular mastitis: a comparison of mNGS and culture by Xu Mu, Xu Mu, Hongmin Luo, Hanhua Li, Shenghua Chen, Shenghua Chen, Yuyang Han, Yuyang Han, Lin Zhang, Lin Zhang, Wei Liu, Weilong Qiao, Shaoyi Zheng, Zhifeng Huang, Zhifeng Huang, Zhifeng Huang

“…After antibiotic treatment, the pathogen detection rate of metagenomic next-generation sequencing (mNGS) (54.55%) was found to be higher than that of culture-based methods (27.27%). …”
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A novel variation in RSPO4 causing nonsyndromic congenital nail disorder-4 in a Chinese patient by Qiang Zhang, Qiang Zhang, Qiang Zhang, Qiang Zhang, Qiang Zhang, Qi Yang, Qi Yang, Qi Yang, Qi Yang, Xunzhao Zhou, Xunzhao Zhou, Xunzhao Zhou, Xunzhao Zhou, Sujie Zhang, Sujie Zhang, Sujie Zhang, Sujie Zhang, Jingsi Luo, Jingsi Luo, Jingsi Luo, Jingsi Luo

“…Sanger sequencing validated the variant and determined its origin. …”
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GPC3: a novel mutated gene in pleuropulmonary blastoma by Jiahang Zeng, Xinying Wang, Yingyi Xu, Qinglin Yang, Junzheng Peng, Fenghua Wang, Jue Tang, Dongmei Huang, Jiachi Liao, Yunyu He, Lin Huang, Jianhua Liang, Le Li, Zefeng Lin

“…This study identifies a novel mutation in the GPC3 gene and evaluates its potential as a prognostic biomarker in PPB. …”
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Improving doublet cell removal efficiency through multiple algorithm runs by Yong She, Chaoye Wang, Qi Zhao

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Different artificial feeding strategies shape the diverse gut microbial communities and functions with the potential risk of pathogen transmission to captive Asian small-clawed ott... by Yuanda Gao, Hangyu Zhang, Dapeng Zhu, Long Guo

“…The objective is to evaluate the impacts of two different artificial diets on microbial communities and function capabilities and short-chain fatty acid (SCFA) profiles in healthy otters’ feces. …”
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Fulvic acid exhibits antitumor effects in ovarian cancer cells by upregulating cytochrome P450 family 1 subfamily A member 1 expression by Min Chen, Ming Cheng, Chenchen Shao, Wenwen Liang, Yi Tang, Fangjun Ding

“…The effects of FA and expression of cytochrome P450 family 1 subfamily A member 1 (CYP1A1) on cellular proliferation, migration, and invasion were evaluated using the Cell Counting Kit-8 and transwell assays for migration and invasion. …”
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Genomic epidemiology of antimicrobial resistance determinants in Chinese swine farm Escherichia coli isolates by Zizhe Hu, Yee Huang, Xuemei Cui, Tuanyuan Shi, Quanan Ji, Xiangru Wang, Guolian Bao, Yan Liu

“…The phenotypic and genotypic characteristics of the isolates were comprehensively analyzed using whole-genome sequencing.Results227 slurry samples were collected from 52 pig farms located across 31 provinces in seven geographical regions of China. 142 non-duplicate strains of E. coli were isolated, and their resistance phenotypes to 28 antimicrobials were systematically evaluated. …”
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From wastewater to resistance: characterization of multidrug-resistant bacteria and assessment of natural antimicrobial compounds by Mingyue Li, Mingyue Li, Mingyue Li, Angela Zhan, Angela Zhan, Angela Zhan, Tahira Tasneem Rahman, Tahira Tasneem Rahman, Tao Jiang, Liyuan Hou, Liyuan Hou

“…The isolates were identified using 16S rRNA gene sequencing and whole-genome sequencing (WGS), and their antibiotic susceptibility profiles were evaluated. …”
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The osteosarcoma immune microenvironment in progression: PLEK as a prognostic biomarker and therapeutic target by Yunpeng Zou, Yunpeng Zou, Jianning Kang, Shaopeng Zhu, Xuechen Ren, Zheng Li, Jiayao Niu, Xuanzhe Qin, Hongbo Li, Lu Xiang, Wei Jiang, Jiangbo Zhong, Ying Zhang, Ying Zhang, Kai Zhao

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Integrated analyses uncover new features of atypical memory B cells and novel targets for intervention by Fuli Fan, Shubei Liu, Bin Wang, Xiaojian Song, Wei Wang

“…Method: B cell subsets and their transcriptomic signatures were identified via analyses of single cell RNA-sequencing (scRNA-seq) data. Functional characterization of AMB was performed with bioinformatics and CyTOF-based phenotyping. …”
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Long-term alterations in gut microbiota following mild COVID-19 recovery: bacterial and fungal community shifts by Da Li, Da-Ya Zhang, Shi-Ju Chen, Yan-Ting Lv, Shi-Mei Huang, Chen Chen, Fan Zeng, Run-Xiang Chen, Xiao-Dong Zhang, Jian-Xin Xiong, Fa-Di Chen, Yue-Hong Jiang, Zhai Chen, Cui-Yi Mo, Jia-Jia Chen, Xu-Li Zhu, Li-Jun Zhang, Fei-Hu Bai, Fei-Hu Bai

“…A random forest model evaluated the predictive capacity of key taxa.ResultsThe gut microbiota of COVID-19 recoverees differed significantly from that of healthy individuals. …”
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Prenatally and postnatally sequential genetic etiology detection in corpus callosum abnormalities by Huili Xue, Aili Yu, Lin Zheng, Xianglan Ye, Lin Zhang, Qun Guo, Lingji Chen, Qingmei Shen, Na lin, Hailong Huang, Liangpu Xu

“…Abstract To evaluate the clinical utility of genetic testing via karyotyping, chromosomal microarray analysis (CMA), and exome sequencing in cases with corpus callosum abnormalities (CCA). …”
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Characteristic alterations of gut microbiota and metabolites in patients with perianal abscess: a multi-omics analysis by Huangfu Ma, Jingjing Fang, Mengjie Li, Yige Qin, Yanmei Wang, Seong-Gook Kang, Kunlun Huang, Kunlun Huang, Kunlun Huang, Tao Tong, Tao Tong, Tao Tong

“…First, 16S rRNA sequencing was used to evaluate differences in the gut microbiota between children with PA and the HC group, and 10 children with PA and 10 children in the HC group were randomly selected for non-targeted metabolomic analysis.ResultsThere were significant differences in the gut microbiota diversity and composition between children with PA and HC. …”
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Genomic insights into growth traits in German Black Pied cattle: a dual-purpose breed at risk by P. Korkuć, M. Reißmann, G.A. Brockmann

“…Using imputed whole-genome sequencing data, we identified 14 quantitative trait loci across ten chromosomes. …”
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miRNA-541-5p regulates myocardial ischemia–reperfusion injury by targeting ferroptosis by ZhiYu Zhao, BoXia Li, DianWei Cheng, YuFang Leng

“…ELISA kits quantified MDA, SOD, GSH, LDH, and ferritin levels. TTC staining evaluated infarction size. HE staining observed histopathological changes. …”
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Association of psychosocial factors and biological pathways identified from rare-variant analysis with longitudinal trajectories of treatment response in major depressive disorder by Haiping Tang, Yan Xia, Chenjie Gao, Yufan Cai, Yongqi Shao, Wenji Chen, Yonggui Yuan, Chunyu Liu, Zhijun Zhang, Zhi Xu

“…The impact of psychosocial factors—including childhood trauma experience, social support, and family environment—on these response patterns was evaluated using ANOVA and Tukey’s HSD tests. Additionally, targeted exome sequencing was conducted to perform rare-variant burden and enrichment analyses to investigate genetic influences on antidepressant response. …”
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TCL1A in naïve B cells as a therapeutic target for type 1 diabetesResearch in context by Siweier Luo, Lina Zhang, Chunfang Wei, Chipeng Guo, Zhe Meng, Honghui Zeng, Lele Hou, Le Wang, Zulin Liu, Yufei Du, Shiyu Tan, Yating Zhang, Xiaoding Xu, Liyang Liang, Yiming Zhou

“…Furthermore, we developed a siRNA-based nanomedicine and evaluated its therapeutic potential in the NOD mouse. …”
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Transcriptomic Changes and Pathological Mechanisms in Familial and Sporadic Idiopathic Restless Legs Syndrome: Implications for Inflammation and Cell Adhesion Molecules by He XR, Song JM, Zhao JP, Zhang J, Feng JT, Chen SQ, Zhou ZY, Wang HM, Zhang Y, Feng Y, Wu YC, Zhu XY

“…Messenger RNA (mRNA) extracted from the peripheral blood mononuclear cells of these participants was analyzed via next-generation sequencing, followed by GO and KEGG pathway analyses. …”
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Profiling of the serum MiRNAome in pediatric egyptian patients with wilms tumor by Fatma S. Mohamed, Fatma S. Mohamed, Deena Jalal, Youssef M. Fadel, Samir F. El-Mashtoly, Wael Z. Khaled, Wael Z. Khaled, Ahmed A. Sayed, Ahmed A. Sayed, Mohamed A. Ghazy, Mohamed A. Ghazy

“…This study aimed to profile aberrantly expressed miRNAs in WT serum samples, evaluate their potential to differentiate standard-risk patients with favorable histology from those with anaplastic WTs, and assess their clinical value as minimally invasive biomarkers for WT detection. …”
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