Prevalence and molecular characteristics of colistin-resistant isolates among carbapenem-resistant Klebsiella pneumoniae in Central South China: a multicenter study by Zijuan Jian, Yanjun Liu, Zhiqian Wang, Peilin Liu, Jiahui Wang, Qun Yan, Wenen Liu

“…The bacterial virulence was evaluated through a Galleria mellonella larvae infection model. …”
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Long-term effects of combining anaerobic digestate with other organic waste products on soil microbial communities by Daniela Mora-Salguero, Denis Montenach, Manon Gilles, Vincent Jean-Baptiste, Sophie Sadet-Bourgeteau, Sophie Sadet-Bourgeteau

“…Soil microbial communities were characterized using high-throughput sequencing targeting 16S and 18S ribosomal RNA genes, following DNA extraction from soil samples collected in 2022, six years after the initial digestate application.ResultsThe results indicated that combining OWPs rich in stable and recalcitrant organic matter, such as BIO and FYM, with raw digestate, offers an improved fertilization practice. …”
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Clinical and genetic drivers of oligo-metastatic disease in colon cancer by Alessandro Ottaiano, Mariachiara Santorsola, Roberto Sirica, Annabella Di Mauro, Antonella Di Carlo, Monica Ianniello, Francesco Sabbatino, Rosa Castiello, Francesca Del Peschio, Marco Cascella, Francesco Perri, Maurizio Capuozzo, Nicola Martucci, Edoardo Mercadante, Valentina Borzillo, Rossella Di Franco, Francesco Izzo, Vincenza Granata, Carmine Picone, Antonella Petrillo, Massimiliano Berretta, Salvatore Stilo, Luca Tarotto, Anna Chiara Carratù, Gerardo Ferrara, Madhura Tathode, Alessia Maria Cossu, Marco Bocchetti, Michele Caraglia, Guglielmo Nasti, Giovanni Savarese

“…Associations with metastatic patterns were evaluated using Chi-square. Results: The analysis involved 104 patients (44 with OMD, 60 with PMD). …”
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Isolation, characterization, and genomic analysis of a novel bacteriophage vB_Kp_XP4 targeting hypervirulent and multidrug-resistant Klebsiella pneumoniae by Xiaocui Peng, Xiaocui Peng, Jianliang Chang, Jianliang Chang, Hongxia Zhang, Xiaoyu Li, Xiaoyu Li, Changhong Zhang, Shiyan Jiao, Chengxiu Lv, Na Wang, Jun Zhao, Jun Zhao, Bu Wang, Wei Zhang, Zhihua Zhang

“…The complete genome sequence has been submitted to GenBank under accession number PP663283.…”
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Real-world treatment patterns and outcomes among patients initiating sequential regorafenib and trifluridine/tipiracil ± bevacizumab in patients with metastatic colorectal cancer i... by Tanios Bekaii-Saab, Ila Sruti, Junxin Shi, Wei Dai, Gregory Patton, Sreevalsa Appukkuttan, Brian Hocum, Arvind Katta, Svetlana Babajanyan, David Cosgrove

“…The Kaplan–Meier methods were used for time-to-event endpoints, including real-world overall survival (rwOS), real-world progression-free survival (rwPFS), and real-world time to next treatment (rwTTNT) following sequence. Endpoints were also evaluated using Cox proportional hazards models.ResultsThis study examined 308 patients initiating sequential regorafenib and FTD/TPI, 156 patients initiating regorafenib first (R-T), and 152 patients initiating FTD/TPI first (T-R). …”
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Genomic analysis of extended-spectrum beta-lactamase-producing E. coli from Czech diary calves and their caretakers by Martina Masarikova, Aneta Papouskova, Iva Sukkar, Iva Sukkar, Jarmila Lausova, Darina Cejkova, Alois Cizek, Alois Cizek

“…Using whole-genome sequencing, we evaluated resistance genotypes, sequence types, serotypes, plasmid replicons, and virulence genes.Results and discussionAmong 266 rectal samples obtained from the calves, 128 (48%) harbored cefotaxime-resistant E. coli. …”
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Whole-genome analysis of escherichia coli isolated from captive giant pandas (ailuropoda melanoleuca) at the Dujiangyan base of the China conservation and research center for the g... by Hongyan Yu, Mengru Zhao, Maolin Lu, Hongjia Li, Shu Fang, Ruisi Zhang, Tianlu Liu, Zhiyou Lü, Mengchao Zhou, Yaxian Lu, Tongzuo Zhang, Zhijun Hou

“…Core genome SNP analysis was performed to establish genetic relationships, and AST was conducted to evaluate antibiotic resistance profiles. Whole-genome sequencing revealed 15 distinct sequence types (STs), with ST48 (22.7%, 5 isolates) and ST212 (18.2%, 4 isolates) identified as the dominant lineages. …”
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Interpreting the clinical significance of multiple large-scale mitochondrial DNA deletions (MLSMD) in skeletal muscle tissue in the diagnostic evaluation of primary mitochondrial d... by Jing Wang, Jing Wang, James T. Peterson, Joaquim Diego D. Santos, Ada J. S. Chan, Maria Alejandra Diaz-Miranda, Imon Rahaman, Jean Flickinger, Amy Goldstein, Amy Goldstein, Emily Bogush, Elizabeth M. McCormick, Colleen C. Muraresku, Vernon E. Anderson, Matthew C. Dulik, Matthew C. Dulik, Douglas C. Wallace, Douglas C. Wallace, Rui Xiao, Marni J. Falk, Marni J. Falk, Angela N. Viaene, Angela N. Viaene, Zarazuela Zolkipli-Cunningham, Zarazuela Zolkipli-Cunningham, Zarazuela Zolkipli-Cunningham

“…We sought to evaluate clinical, biochemical, and histopathological phenotypes of a large clinical cohort harboring MLSMD in muscle to better understand their significance across a range of clinical phenotypes.MethodsA single-site retrospective study was performed of 212 diagnostic muscle biopsies obtained from patients referred for Primary Mitochondrial Disease (PMD) evaluation with muscle mitochondrial (mt)DNA sequencing performed at our institution, including electronic medical record (EMR) review of symptoms, biochemical results, and Mitochondrial Myopathy Composite Assessment Tool (MM-COAST) scores.ResultsMLSMD were identified in 50 of 212 (24%) diagnostic tissue biopsies, and were universally present. in subjects ≥50 years (n = 18/18). …”
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Design and expression of a chimeric recombinant antigen (SsIR-Ss1a) for the serodiagnosis of human strongyloidiasis: Evaluation of performance, sensitivity, and specificity. by Mostafa Omidian, Zohreh Mostafavi-Pour, Marzieh Asadi, Meysam Sharifdini, Navid Nezafat, Ali Pouryousef, Amir Savardashtaki, Mortaza Taheri-Anganeh, Fattaneh Mikaeili, Bahador Sarkari

“…The solubility and physicochemical properties of the designed construct were analyzed and its tertiary structures were built and evaluated. The construct was expressed into the pET-23a (+) expression vector and the optimized DNA sequences of SsIR-Ss1a (873 bp) were cloned into competent E. coli DH5α cells. …”
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An automated predictive model for evaluating narrative cohesion in children’s stories: a computational linguistic approach considering Gérard Genette’s narrative structure theory... by Jawharah Alasmari, Mohammed Alzyoudi, Masheal Alshehri, Rana Alshammari, Reyouf Aldakan

“…It analyzes both human-created and AI-generated stories, including those from ChatGPT, by assessing linguistic, rhetorical, and stylistic elements such as narrative style, character development, time specification, event sequencing, and dialogue. The study employs a Decision Tree model to evaluate narrative cohesion, achieving optimal results with both recall and precision at 100%. …”
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Biological Characterization and Evaluation of the Therapeutic Value of <i>Vibrio</i> Phages 4141 and MJW Isolated from Clinical and Sewage Water Samples of Kolkata by Sanjoy Biswas, Devendra Nath Tewari, Alok Kumar Chakrabarti, Shanta Dutta

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Therapeutic Evaluation <i>Punica granatum</i> Peel Powder for the Ailment of Inflammatory Bowel Disorder in NCM460 Cell Line and in Albino Rats by Parikshit Roychowdhury, Gyanendra Kumar Prajapati, Rupesh Singh, Prasanna Gurunath, Ramesh C, Gowthamarajan Kuppuswamy, Anindita De

“…Transcriptomic sequencing identified downregulation of the IL-6/STAT3 signaling pathway and reduced inflammatory markers like p-NF-κB, IL-1β, and NLRP3 on PPPG therapy. …”
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Evaluation of Faecal Microbiota Following Probiotics in Infants of Mothers with Gestational Diabetes Mellitus Trial: Protocol for Double-Blind Placebo-Controlled Randomized Trial by Gayatri Athalye-Jape, Chandra Prakash Rath, Harshad Panchal, Archita Mishra, Dorothy Graham, Sanjay Patole

“…To best of our knowledge, no study has evaluated the effects of probiotics on gut dysbiosis in the infants of mothers with GDM. (2) Methods: This study will be a single-centre, double-blind, randomized, placebo-controlled trial enrolling sixty neonates born after 35 weeks of gestation to mothers with GDM. …”
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Safety evaluation of the food enzyme non‐reducing end α‐l‐arabinofuranosidase from the non‐genetically modified Aspergillus tubingensis strain ARF by EFSA Panel on Food Enzymes (FEZ), Holger Zorn, José Manuel Barat Baviera, Claudia Bolognesi, Francesco Catania, Gabriele Gadermaier, Ralf Greiner, Baltasar Mayo, Alicja Mortensen, Yrjö Henrik Roos, Marize L. M. Solano, Monika Sramkova, Henk Van Loveren, Laurence Vernis, Cristina Fernàndez‐Fraguas, Magdalena Andryszkiewicz, Jaime Aguilera, Daniele Cavanna, Ana Criado, Elsa Nielsen, Karin Nørby, Yi Liu

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Evaluation of chitinase production ability to antagonize Pyricularia Oryzae of bacteria and actinomycete strains isolated from rice root soil in Kien Giang Province by Đinh Anh Hòa, Trần Thị Phấn, Trần Thùy Trang, Nguyễn Thị Ánh Nguyệt, Nguyễn Thị Thùy Dương, Nguyễn Hải An, Lê Thị Mai Châm

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Evaluation of high-moisture oat silage inoculated with synthetic lactic acid bacteria consortia in mini-silos: fermentation, microbial, metabolic and safety profiles by Cheng Zong, Longxin Wang, Jie Zhao, Zhihao Dong, Junfeng Li, Xianjun Yuan, Chengti Xu, Tao Shao

“…This work evaluated the effects of five synthetic lactic acid bacteria (LAB) consortia (CM1-CM5) on the fermentation, microbial, metabolic, and safety profiles of high-moisture oat silage. …”
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Evaluation of the MDM-score system for screening mitochondrial diabetes mellitus in newly diagnosed diabetes patients: a multi-center cohort study in China by Fuhui Ma, Jing Zhao, Yan Chen, Yunzhi Luo, Yuxuan Du, Xia Li, Tao Xu, Zhiguang Zhou, Kaixin Zhou, Kaixin Zhou, Yanying Guo

“…Their DNA samples were subjected to targeted sequencing of 37 genes, including the mitochondrial m.3243A&gt;G mutation. …”
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Evaluation of Stress-Tolerant <i>Serratia</i> and <i>Enterobacter</i> as PGPR for Nutrient Solubilization and Dose-Dependent Bioformulation to Enhance Tomato Seedlings by Indu Bhardwaj, Vijay Kumar, Somvir Singh, Arti Jamwal Sharma, Shikha Kumari, Nidhi Bhardwaj, Kanika Dulta, Lukas Peter, Richa Verma, Nitesh Kumar, Yogesh K. Ahlawat, Anurag Malik, Mohammad K. Okla, Rosa Porcel, José M. Mulet, Karthikeyan Jayabalan

“…Molecular identification via 16S rRNA gene sequencing confirmed that these isolates belong to the genera <i>Serratia</i> and <i>Enterobacter</i>. …”
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Phytosanitary problems in elephant garlic (Allium ampeloprasum var. holmense) in the “Val di Chiana” area (Central Italy), and evaluation of potential control strategies by Francesco TINI, Giovanni BECCARI, Niccolò TERZAROLI, Enrica BERNA, Lorenzo COVARELLI, Mara QUAGLIA

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In vivo evaluation of phage therapy against Klebsiella pneumoniae using the Galleria mellonella model and molecular characterization of a novel Drulisvirus phage species by Gustavo Quispe-Villegas, Gabriela I. Alcántara-Lozano, Diego Cuicapuza, Raúl Laureano, Brenda Ayzanoa, Pablo Tsukayama, Jesús Tamariz

“…Viral kinetics and phage stability were evaluated under varied pH and temperature conditions. …”
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