Comparative Analysis of Metagenomic Next-Generation Sequencing, Sanger Sequencing, and Conventional Culture for Detecting Common Pathogens Causing Lower Respiratory Tract Infection... by Qiaolian Yi, Ge Zhang, Tong Wang, Jin Li, Wei Kang, Jingjia Zhang, Yali Liu, Yingchun Xu

“…The detection performance was compared between mNGS and standard microbiology culture, using Sanger sequencing as the reference method. 52.05% (165/317) of sputa showed identical results for all three methods. …”
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Spatial and temporal detection of 'Candidatus Liberibacter asiaticus' in Diaphorina citri through optimized scouting, sampling, DNA isolation, and qPCR amplification in California... by Nathaniel Ponvert, Frank Byrne, Monique J Rivera, Timo Rohula, Sandra Olkowski, Heshani De Silva Weligodage, Neil McRoberts, Judith K Brown

“…Results indicated that CLas detection correlated with psyllid developmental stage, with the 4th-5th instar nymphs (sample size of five to ten per tube) or adult ACP (sample size of three to ten per tube) providing the most consistent qPCR detection. …”
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MEFA-Net: Multilevel Feature Extraction and Fusion Attention Network for Infrared Small-Target Detection by Jingcui Ma, Nian Pan, Dengyu Yin, Di Wang, Jin Zhou

“…Compared with the baseline, the intersection over union (IoU) and probability of detection <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mrow><mi>P</mi></mrow><mrow><mi>d</mi></mrow></msub></mrow></semantics></math></inline-formula> of MEFA-Net on the IRSTD-1k dataset are increased by 2.25% and 3.05%, respectively, achieving better detection performance and a lower false alarm rate in complex scenarios.…”
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Diagnostic Accuracy of 3 Mpox Lateral Flow Assays for Antigen Detection, Democratic Republic of the Congo and United Kingdom by Elie Ishara-Nshombo, Anushri Somasundaran, Alessandra Romero-Ramirez, Konstantina Kontogianni, Daniel Mukadi-Bamuleka, Marithé Mukoka-Ntumba, Emile Muhindo-Milonde, Hugues Mirimo-Nguee, Jacob Parkes, Yusra Hussain, Susan Gould, Christopher T. Williams, Dominic Wooding, Juvenal Nkeramahame, Mikaela Watson, Hayley E. Hardwick, Malcolm G. Semple, John Kenneth Baillie, Jake Dunning, Thomas E. Fletcher, Thomas Edwards, Devy M. Emperador, Hugo Kavunga-Membo, Ana Isabel Cubas-Atienzar

“… The ongoing outbreaks of mpox highlight the urgent need for a rapid and low-cost diagnostic test to accurately detect and control this emerging disease. We estimated the analytical sensitivity using viral culture of the monkeypox virus clade IIb lineage B1 and clinical diagnostic performance of 3 antigen detection rapid diagnostic tests (Ag-RDT) by using skin swab samples and upper-respiratory swab samples from mpox patients in the Democratic Republic of the Congo and the United Kingdom. …”
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DNMT3A GENE POINT MUTATIONS DETECTION IN ACUTE MYELOID LEUKEMIA PATIENTS USING SEQUENCING TECHNIQUE by A. V. Vinogradov, A. V. Rezaykin, A. G. Sergeev

“…Total RNA was extracted from leukemic cells and subjected to reverse transcription. DNMT3A gene exons 18–26 were amplified by PCR. Detection of mutations in DNMT3A gene was performed by direct sequencing. …”
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Enzymeless electrochemical detection of hydrogen peroxide using NiO octahedron decorated 3D graphene hydrogel by Mohamed A. Yassin, Ayman F. Abou-Hadid, Hamouda M. Mousa, Chan Hee Park, Cheol Sang Kim, Ali Salem, Mohamed A. Mattar

“…Finally, the analytical utilities of the 3DGH/NiO25 proposed electrode were conducted to detect H2O2 in real products of milk samples.…”
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3D Bioprinted Hydrogel Sensor towards Rapid Salivary Diagnostics Based on pH Colorimetric Detection by Magdalena Beata Łabowska, Agnieszka Krakos (Podwin), Wojciech Kubicki

“…In this paper, a novel solution of 3D bioprinted hydrogel sensor towards rapid diagnostics of saliva based on pH colorimetric detection is proposed. …”
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An RPA-Based CRISPR/Cas12a Assay in Combination with a Lateral Flow Assay for the Rapid Detection of <i>Shigella flexneri</i> in Food Samples by Jieru Xu, Tianxin Zhang, Xinrui Lv, Lei Shi, Weibin Bai, Lei Ye

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Design of Potentiometric Sensor Based on Molecularly-imprinted Polymer for Direct Detection of Levofloxacin in Plasma for Point-of-Care Applications by Amr M. Mahmoud, Hanaa Saleh, Michael Gamal Fawzy, Alaa Reda, Eman A. Bahgat

“…Results: the sensor responded quickly within 3-5 seconds in the pH range (3.0 – 6.0). Within the concentration range of (1 × 10-5 - 1 × 10-2 M), the potential profile showed a linear relationship with limit of detection (LOD) of 7.41 × 10-6 M. …”
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Real-Time PCR for Detection CP1 & CP5 Virulence Factors of Entamoeba Histolytica in Patients Stool Samples in Al - Najaf Al- Ashraf Province by Haiytham Mohamed Hamady, Zainab Ali Hussein

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An Efficient and Selective 7‑(Diethylamino)quinolin-2(1H)‑One-Chalcone Fluorescent Probe for Detecting Bisulfite in Wine Samples Using a Micellar Solution by Guillermo E. Quintero, William Tiznado, Luis Leyva-Parra, Catalina Espinoza, Oriel Sánchez-Velasco, Edwin G. Pérez, Carlos Rojas-Romo, Christian Espinosa-Bustos, Margarita E. Aliaga

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Novel Quantification Method for Lithium Ion Battery Electrolyte Solvents in Aqueous Recycling Samples Using Solid‐Phase Extraction/Gas Chromatography‐Flame Ionization Detection by Julius Buchmann, Yixin Song, Simon Wiemers‐Meyer, Martin Winter, Sascha Nowak

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IVF cycle safety when a positive passive air sampling occurs under laminar flow hood in absence of a detectable contamination in the embryo culture by Claudia Omes, Roberto Bassani, Patrizia Cambieri, Fausto Baldanti, Fausto Baldanti, Rossella Elena Nappi, Rossella Elena Nappi

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High-Performance ZIF-7@PANI Electrochemical Sensor for Simultaneous Trace Cadmium and Lead Detection in Water Samples: A Box–Behnken Design Optimization Approach by Sondes Guesmi, Nashwan H. Ali, Nadhem Missaoui, Zouhaier Aloui, Chama Mabrouk, Carlos Castilla Martinez, Fraj Echouchene, Houcine Barhoumi, Nicole Jaffrezic-Renault, Hamza Kahri

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Improved limit of detection for zoonotic Plasmodium knowlesi and P. cynomolgi surveillance using reverse transcription for total nucleic acid preserved samples or dried blood spots... by Kamil A Braima, Kim A Piera, Inke N D Lubis, Rintis Noviyanti, Giri S Rajahram, Pinkan Kariodimedjo, Irbah R A Nainggolan, Ranti Permatasari, Leily Trianty, Ristya Amalia, Sitti Saimah Sakam, Angelica F Tan, Timothy William, Jacob A F Westaway, PingChin Lee, Sylvia Daim, Henry Surendra, Nathaniel Christy, Andrew G Letizia, Christopher L Peatey, Mohd Arshil Moideen, Bridget E Barber, Colin J Sutherland, Nicholas M Anstey, Matthew J Grigg

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Tree stocking affects winter bird densities across a gradient of savanna, woodland, and forest in the Missouri Ozarks by Sarah W. Kendrick, Frank R. Thompson III

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A fluorescence probe for monitoring toxic hypochlorous acid in biosystems and environmental waters with a broad pH adaptation by Junyan Ma, Zilin Jiao, Mingtao Zhao, Xiangtao Kong, Hua Xie, Zhenxing Zhang

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Evolutionary variation of the monkeypox virus detected for the first time in Nantong, Jiangsu by Xingpei Ji, Ruyue Liang, Chao Bao, Xiuli Cai, Shiyao Chen, Lu Chen, Li Li, Xiaolei Ji

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Simultaneous Trace Analysis of Lead and Cadmium in Drinking Water, Milk, and Honey Samples Through Modified Screen-Printed Electrode by Fei Wang, Xiao Peng, Ziqian Xiao, Ying Ge, Bilin Tao, Zhaoyong Shou, Yifei Feng, Jing Yuan, Liang Xiao

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Performance Evaluation of a Fully Automated Molecular Diagnostic System for Multiplex Detection of SARS-CoV-2, Influenza A/B Viruses, and Respiratory Syncytial Virus by James G. Komu, Dulamjav Jamsransuren, Sachiko Matsuda, Haruko Ogawa, Yohei Takeda

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