Development of a SYBR Green-Based Real-Time PCR Assay to Detect <i>Oncomelania hupensis quadrasi</i> DNA in Environmental Water Samples by Daria L. Manalo, Jude Karlo G. Bolivar, Karl Ian T. Ermino, Jeromir G. Bondoc, Mark Joseph M. Espino, Efraim P. Panganiban, Kathyleen S. Nogrado, Raffy Jay C. Fornillos, Mario A. Jiz, Lydia R. Leonardo, Ian Kendrich C. Fontanilla

Get full text

Assessment of Exhaled Breath Condensate for ALK, RET, ROS1, and NTRK1 Fusion Transcript Detection in NSCLC: Comparison With Tissue and Liquid Biopsy Samples by Aslı Tetik Vardarlı, Haydar Soydaner Karakus, Korcan Korba, Burcu Boluk, Su Ozgur, Cumhur Gunduz, Fusun Pelit, Ali Veral, Tuncay Goksel

Get full text

Technical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans by Szabolcs Maar, Lilla Czuni, Jørgen Kosberg Hassve, Aniko Takatsy, Szilard Rendeki, Tibor Mintal, Ferenc Gallyas, Jr., Ildiko Bock-Marquette

Get full text

Methodological establishment and diagnostic value of a multiplex fluorescent PCR assay for the detection of three fastidious respiratory pathogens. by Jingchao Shi, Yijun Zhu, Shuyun Chen, Xiaoyun Shan, Lihong Bo, Kai Shen, Keqiang Chen

“…For samples with inconsistent detection results, targeted high-throughput sequencing (tNGS) was used for confirmation.…”
Get full text

A 3D-printed handheld device for quick citrus tissue lysis and nucleic acid extraction by Chia-Wei Liu, Brent Kalish, Sohrab Bodaghi, Georgios Vidalakis, Hideaki Tsutsui

“…Abstracts: A 3D-printed handheld device has been developed for rapid and efficient sample preparation, extracting pathogen total nucleic acids from citrus leaves for downstream molecular analysis. …”
Get full text

Portable SpectroChip-Based Immunoassay Platform for Rapid and Accurate Melamine Quantification in Urine Samples by Cheng-Hao Ko, Wei-Yi Kong, Abel Chernet Kabiso, Wei-Huai Chiu, Ashenafi Belihu Tadesse, Chitsung Hong, Chia-Fang Wu, Hung-Hsun Lin

Get full text

Microplastic detectives: a citizen-science project reveals large variation in meso- and microplastic pollution along German coastlines by Bruno Andreas Walther, Franco Pasolini, Franco Pasolini, Špela Korez Lupše, Melanie Bergmann

“…Here, we describe in detail 1) how we recruited, instructed, and engaged citizen scientists, 2) why we chose bulk sampling over reduced-volume sampling, and 3) the laboratory methods we used. …”
Get full text

Probe – Integrated electrochemical sensing platform for detection of trace levels of Dapoxetine hydrochloride drug residue in water and drug formulations by H. Alwael, N.A. Asiri, B.G. Alhogbi, A.S. Alharthi, E.A. Bahaidarah, T.N. Abduljabbar, D.F. Baamer, M. Madkour, F.A. Zainy, M.S. El-Shahawi

“…The probe also has favorable LOD and good LDR compared to the recent reported assays for DPH detection. The probe was applied for detection of DPH in its drug formulations and its residues in water samples with acceptable recoveries (98.5 ± 0.014–104 ± 0.016 %) with favorable repeatability and reproducibility. …”
Get full text

Development of COPMAN-Air method for high-sensitivity detection of SARS-CoV-2 in air by Tomoyo Yoshinaga, Yoshinori Ando, Yumi Sato, Takeru Kishida, Masaaki Kitajima

“…We applied COPMAN to air samples, thereby developing COPMAN-Air. Briefly, this method efficiently detects the extremely low levels of viral RNA in air samples via three reaction steps: RT, preamplification, and qPCR, as it is performed with COPMAN. …”
Get full text

Environmental DNA (eDNA) as a tool to detect Arctic grayling and their habitat preferences in the Northwest Territories, Canada by Heather D. Veilleux, Melissa D. Misutka, Morag D. McPherson, Peter A. Cott, Greg G. Goss, Mark Poesch, Chris N. Glover

Get full text

Enhanced small-scale APT knowledge graph embedding via spatio-temporal attribute reasoning and adversarial negative sampling by Yushun Xie, Haiyan Wang, Xiao Jing, Zhaoquan Gu

Get full text

Development of a graphene field effect transistor-based immersible biosensor for immunodetection of the birch pollen allergen Bet v 1 in air samples by Stefan Jarić, Mario Wenger, Ivan Bobrinetskiy, André Stapelfeldt, Glorismer Pena-Amelunxen, Branko Šikoparija, Lorenz Aglas

Get full text

Pathogen detection and antibiotic use in granulomatous lobular mastitis: a comparison of mNGS and culture by Xu Mu, Xu Mu, Hongmin Luo, Hanhua Li, Shenghua Chen, Shenghua Chen, Yuyang Han, Yuyang Han, Lin Zhang, Lin Zhang, Wei Liu, Weilong Qiao, Shaoyi Zheng, Zhifeng Huang, Zhifeng Huang, Zhifeng Huang

Get full text

A Metric Learning-Based Improved Oriented R-CNN for Wildfire Detection in Power Transmission Corridors by Xiaole Wang, Bo Wang, Peng Luo, Leixiong Wang, Yurou Wu

Get full text

Detection of autoimmune thyroid diseases in patients with celiac disease by Rana S. Aboud, Haidar J. Mohammad, Nawal M. Jawad

“…IgA and IgG antigliadin antibodies were measured in serum with celiac disease sample. The patients which were positive to gliadin antibody were carried out to T3, T4 and TSH by ELISA tests. …”
Get full text

Molecular Detection of Neiseria Gonorrheae and Chlamydia Trichomatis Among Infertile Male Client Attending some Health Facilities in the Northwest, Nigeria by Dogo Ezekiel Bitet, Abdulhadi Sale Kumurya, Abubakar Isah Aliyu, Bawa Ega, Joseph M Ajagbe

“…Methods: A total of 383 infertile male semen samples were collected for this study. C. trachomatis and N. gonorrhoeae infections were molecularly detected in the semen samples using the multiplex method. …”
Get full text

The current state of DNA methylation biomarkers in self-collected liquid biopsies for the early detection of cervical cancer: a literature review by Elizabeth G. Sumiec, Zhe Yang Yim, Hannah Mohy-Eldin, Belinda Nedjai

Get full text

Day-to-day variability in activity levels detects transitions to depressive symptoms in bipolar disorder earlier than changes in sleep and mood by Abigail Ortiz, Ramzi Halabi, Martin Alda, Alexandra DeShaw, Muhammad I. Husain, Abraham Nunes, Claire O’Donovan, Rachel Patterson, Benoit H. Mulsant, Arend Hintze

Get full text

Impact of weaning procedures on Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) circulation in the nursery section by Pia R. Heiselberg, Charlotte Sonne Kristensen, Lise Kirstine Kvisgaard, Lars Erik Larsen

“…In this study, pigs were sampled in three herds that performed “mixed at weaning (MIX)” and three herds that performed “all in/all out at weaning (AIAO)”. …”
Get full text

Sampling for vegetative propagation: A phytosanitary status survey of grapevines collection by One Step RT-PCR method by М. Ізейрай

“…One Step Reverse Transcription Polymerase Chain Reaction assay was used to detect the viral presence. Results showed a high (100%) prevalence of GLRaV3 virus in all of analysed samples, as the most frequent among the two pathogens. …”
Get full text