DEMINERS enables clinical metagenomics and comparative transcriptomic analysis by increasing throughput and accuracy of nanopore direct RNA sequencing by Junwei Song, Li-an Lin, Chao Tang, Chuan Chen, Qingxin Yang, Dan Zhang, Yuancun Zhao, Han-cheng Wei, Kepan Linghu, Zijie Xu, Tingfeng Chen, Zhifeng He, Defu Liu, Yu Zhong, Weizhen Zhu, Wanqin Zeng, Li Chen, Guiqin Song, Mutian Chen, Juan Jiang, Juan Zhou, Jing Wang, Bojiang Chen, Binwu Ying, Yuan Wang, Jia Geng, Jing-wen Lin, Lu Chen

“…We present DEMINERS, a novel DRS toolkit combining an RNA multiplexing workflow, a Random Forest-based barcode classifier, and an optimized convolutional neural network basecaller with species-specific training. …”
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Improving Growth Performance, Biochemical Parameters and Reducing Mortality due to Ascites in Broilers by Propranolol Inclusion by Shahriar Saidian, Somaya Farahmand, Mokhtar Fathi, Salar Saifi

“…On the last day of the experiment (42 d), two birds were randomly selected from each cage and after sampling from the wing vein, they were killed and the ascites index was calculated as the ratio of the weight of the right ventricle to the weight of total ventricles (RV/TV). …”
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Evaluation of the Tolerance of Promising Genotype of Bean (Phaseolus vulgaris Linnaeus) to Two-Spotted Mite (Tetranychus urticae Koch (Acari: Tetranychidae)) in Field Conditions by Sedighe Ashtari, Behrouz Asadi, Hamid Reza Dorri

“…Sampling for damage assessment was carried out weekly from the third leaf stage until the end of the season. …”
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Antibiotics in ambient fine particulate matter from two metropolitan cities in China: Characterization, source apportionment, and health risk assessment by Juan Liu, Qiao Yao, Wenyan Yan, Ke Fang, Runming He, Xiaona Wang, Yu’e Cha, Xiaoyan Yang, Wen Gu, Chao Wang, Yifu Lu, Mingyu Zhao, Yujie Ben, Kai Wang, Zhaomin Dong, Rong Zhang, Hong Chang, Song Tang

“…We investigated the occurrence, sources, environmental driving factors, and health risks of antibiotics in PM2.5 samples collected from Beijing and Shijiazhuang, China, during periods of high air pollution. …”
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Real-world clinical outcomes of tixagevimab/cilgavimab in the Omicron outbreak in China: baseline characteristics and interim analysis of the CLEAR study by Jianhua You, Haidi Wu, Jiaxin Tian, Jianru Wen, Wenbo Shi, Zhi Wang, Yanjun Du, Hongwei Xu, Hanyu Wei, Xiang Li, Wenyan Kang, Min Zhou, Zhidong Gu, Jieming Qu

“…Conclusion Among Chinese patients receiving prophylactic tixagevimab/cilgavimab, the incidence of COVID-19-related hospitalization, ICU admission, or death was low during the Omicron surge. Further randomized controlled trials with larger sample sizes are needed to determine the effectiveness of tixagevimab/cilgavimab in preventing severe COVID-19 outcomes. …”
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Barriers and facilitators to patient utilization of noncommunicable disease services in primary healthcare facilities in Nepal: a qualitative study by Sushmita Mali, Elizabeth C. Rhodes, Chandani Singh Nakarmi, Soniya Shrestha, Aarati Dhakal, Alina Bharati, Anupama Bishwokarma, Asmita Adhikari, Bikram Poudel, Binuka Kulung Rai, Sangita Manandhar, Surakshya KC, Dinesh Timalsena, Sashi Silwal, Meghnath Dhimal, Phanindra Prasad Baral, Felix Teufel, Sanju Bhattarai, Donna Spiegelman, Archana Shrestha

“…Methods We conducted a qualitative study with a 35 purposive sample of patients living with one or more NCDs (hypertension, diabetes, chronic obstructive pulmonary disease (COPD/ asthma) who sought healthcare at primary healthcare facilities in 14 randomly selected districts in seven provinces in Nepal that implemented PEN. …”
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Identificación de personas a través del cotejo de radiografías panorámicas y periapicales. Comparación de características observadas en 30 pacientes Identification of people throug... by Clarisa Yanina Gómez, Pamela Borgo Cosentino, Alan Diego Briem Stamm

“…Materials and methods: This study has a qualitative, observational and cross-sectional design. Radiographic samples from 30 patients were randomly selected from a dental clinic archive database. …”

Maternal plasma microRNA profiles in twin-twin transfusion syndrome and normal monochorionic twin pregnancies by Steven T. Papastefan, Steven T. Papastefan, Morgan M. Langereis, Catherine R. Redden, Daniel R. Liesman, Daniel R. Liesman, Cassandra B. Huerta, Lucas E. Turner, Hee Kap Kang, Bethany T. Stetson, Katherine C. Ott, Katherine C. Ott, William S. Marriott, Joyceline A. S. Ito, Aimen F. Shaaban, Aimen F. Shaaban, Amir M. Alhajjat, Amir M. Alhajjat

“…Therefore, we sought to assess whether non-invasive maternal plasma microRNAs can distinguish between TTTS and normal monochorionic diamniotic (MCDA) twin pregnancies.MethodsMaternal blood samples were collected for patients with normal MCDA twin pregnancies (n = 11) or prior to selective fetoscopic laser photocoagulation (SFLP) for patients with TTTS (n = 36). …”
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Effect of speech therapy on quality of life in patients with spinocerebelar ataxia type 3 by Giovana Diaféria, Silvana Bommarito, Pedro Braga Neto, Sung Woo Park, Marina Padovani, Fernanda Haddad, Leonardo Haddad, Mariana Callil Voos, Hsin Fen Chien, José Luiz Pedroso, Orlando Barsottini

“…Methods All participants were randomly assigned to two groups, an intervention group receiving speech therapy (STG) and a control group (CG). …”
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Improved survival in advanced melanoma patients treated with fecal microbiota transplantation using healthy donor stool in combination with anti-PD1: final results of the MIMic pha... by Wilson H Miller, Rahima Jamal, Karl Belanger, Khashayar Esfahani, Saman Maleki Vareki, Bertrand Routy, Seema Nair Parvathy, Arielle Elkrief, Michael S Silverman, Scott Ernst, Kelly J Baines, Danial Khan Hadi, Behnam Jabbarizadeh, Diane Logan, John Gordon Lenehan

“…Combining first-line anti-PD1 therapy and oral FMT with healthy donor stool in this small cohort was safe and demonstrated an improvement in ORR, mPFS, and mOS, compared with randomized trials. Our sample size was small, and results were only hypothesis generating. …”
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Long-term outcome of using Ara-C or not in children’s APL by Yingjin Zhang, Changwen Xue, Chao Wu, Wenyu Yang, Yao Zou, Ye Guo, Yumei Chen, Xiaojuan Chen, Xiaofan Zhu, Li Zhang

“…Our study found no prognostic significance of Ara-C, this may be related to the small sample size. We still recommend the addition of Ara-C during treatment, which has a more positive impact on early remission and late prognosis of patients.…”
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Dysfunctional terminal differentiation of satellite cells in the wooden breast muscle of Ross 308 broiler chickens by Xin Wang, Tong Xing, Xudong Zhu, Xiaofei Wang, Liang Zhao, Feng Gao, Lin Zhang

“…The superficial cranial region of each PM sample was isolated for quantitative analysis of MuSCs functional markers. …”
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Postauthorization safety study of the DPP-4 inhibitor saxagliptin: a large-scale multinational family of cohort studies of five outcomes by Qing Liu, Sean Hennessy, Qufei Wu, K Rajender Reddy, Arlene M Gallagher, Kevin Haynes, David J Margolis, Brian L Strom, Vincent Lo Re, M Elle Saine, Craig W Newcomb, Jason A Roy, Serena Cardillo, Stephen E Kimmel, Peter P Reese, Andrea J Apter, Harshvinder Bhullar, Daina B Esposito, Dean M Carbonari

“…All eligible adult patients newly prescribed saxagliptin (n=110 740) and random samples of up to 10 matched initiators of non-DPP-4 inhibitor OADs within each data source were selected (n=913 384). …”
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Cohort profile: evaluation of immune response and household transmission of SARS-CoV-2 in Costa Rica: the RESPIRA study by Allan Hildesheim, Tim Waterboer, Julia Butt, Mitchell H Gail, Alejandro Calderón, Rolando Herrero, Carolina Porras, Bernal Cortes, Viviana Loría, Amada Aparicio, Gloriana Barrientos, Daniela Retana, Kaiyuan Sun, Rebeca Ocampo, D. Rebecca Prevots, Michael Zúñiga, Roy Wong-McClure, Melvin Morera, Marco Binder, Arturo Abdelnour, Ruth M Pfeiffer, Cristina Barboza Solís, Romain Fantin, Juan Carlos Vanegas, Rachel Mercado, Carlos Ávila

“…Purpose The RESPIRA cohort aims to describe the nature, magnitude, time course and efficacy of the immune response to SARS-CoV-2 infection and vaccination, population prevalence, and household transmission of COVID-19.Participants From November 2020, we selected age-stratified random samples of COVID-19 cases from Costa Rica confirmed by PCR. …”
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Construction and Preliminary Validation of the Children's Academic Readiness Test to Enter Primary School in Afghanistan Country by Mohammad Naeem Rasooli, Ahmad Abedi, Mohammad Ashori

“…A total of 290 children, including 155 girls and 135 boys, were randomly selected from three districts (Districts 11, 13, and 15) of Kabul using cluster sampling and attended educational centers. …”
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Automatic diagnosis and measurement of intracranial aneurysms using deep learning in MRA raw images by Qingning Yang, Fengxi Chen, Li Li, Rong Zeng, Jiaqing Li, Jingxu Xu, Chencui Huang, Junbang Feng, Chuanming Li

“…The true positive (TP), false positive (FP), false negative (FN), recall, sensitivity, and specificity indices were used to evaluate the diagnosis performance of MR 3DUnet. The two-sample t-test was used to compare the size measurement results of MR 3DUnet and two radiologists. …”
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Virtual reality solution to promote adapted physical activity in older adults: outcomes from VR2Care project exploratory study by Vincenzo De Luca, Malak Qbilat, Alessandra Cuomo, Antonio Bianco, Francesca Cesaroni, Chiara Lanari, Ad van Berlo, Telma Mota, Lucia Pannese, Michael Brandstötter, Matthieu Arendse, Vania Mota, Willeke van Staalduinen, Hugo Paredes, Guido Iaccarino, Guido Iaccarino, Maddalena Illario

“…Within the group, baseline to end of observation differences were assessed by paired sample t-test. A p = 0.05 was considered significant.ResultsNo significant improvements in quality of life and physical activity were found. …”
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Investigating the knowledge attitude and practice of evidence based medicine education in Sudanese medical students: a cross sectional study by Mohammed Hammad Jaber Amin, Mohammed Haydar Awad, Elmontasirbillah Salah Mohamed Ginawi, Amjed Siddig Khalid Mohammed, Eltayep Hasab Elrasoul Noureldaiem Ibrahim, Ola Yaser Mohammed Yassen, Leina Elfatih Salah Mohamed Ali Elomeiri, Aseel Hisham Mohamed Abdelhalim Hakim Hussain, Tasneem Abdelrahman Eltahir, Fatima Saif Alyazal Ahmed Mohmmed, Rayan Abdalla Elawad Wedatalla, Khaild Hayder Awad, Rayan Kamal eldin Mahgoub, Abdalazez Mohamed Ali Karar, Ayat Yousif Mohammed Saeed, Asma Eltayeb Abdalla Mohamed, Rawan Salah Mohamed Ginawi, Fatin Mohamed Kaidan Sidar, Reham Rrdwan Abdalrab Saifalsorori, Othman Ibrahim Abdullah Osman, Mohammed Adil Ibrahim Nouri, Manasik Adam Ahmed Mohamed, Nadreen Babikir Mohammad Ibrahim, Maha Mujtaba Mahmoud Mohamed, Abd Elrahman Ahmed Bashir Mohamed, Faris Mohamed AbdAllah MohamedAhmed, Mohammad Rabie Mohammad Hassan, Shahd Abdelkhalig Abdelrahman Mohamed, Hafiz Elhadi Bashar Hadi, Hanan Mohamed Naeim Gandour Gandour, Nirmeen Ali Farah Ahmed, Noon Mohamed El-Fatih Ahmed, Maria Mohammed AlMogtaba AbdElaziz Osman, Sara Elmuntasir Ibrahim Elhadi

“…Methods The study employed a descriptive cross-sectional online survey on Sudanese medical students via convenience sampling. Data collection spanned from March 10, 2023, to September 18, 2023. …”
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Unraveling the immunological landscape and gut microbiome in sepsis: a comprehensive approach to diagnosis and prognosisResearch in context by Yali Luo, Jian Gao, Xinliang Su, Helian Li, Yingcen Li, Wenhao Qi, Xuling Han, Jingxuan Han, Yiran Zhao, Alin Zhang, Yan Zheng, Feng Qian, Hongyu He

“…Metagenomic sequencing was performed in 41 fecal samples, including 13 septic patients, 10 non-septic ICU patients, and 18 healthy controls. …”
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Impact of three rounds of mass preventive chemotherapy on prevalence and intensities of Schistosoma mansoni infection among an adult population on Ukerewe Island, north-western Tan... by Crecencia E. Chiombola, Erick Simon Mwangoka, Tumaini Baumba, Charles Guya Mkombe, Saskia Kreibich, Christa Kasang, Antje Fuss, Antje Fuss, Andreas Mueller, Andreas Mueller, Paul Alikado Sabuni, Humphrey D. Mazigo, Humphrey D. Mazigo

“…Secondarily, we report on the treatment coverage in all the treatment rounds.MethodsA community-based cross-sectional study was conducted in 20 villages and included randomly selected adults aged ≥15 years. A single stool sample was obtained from each consenting participant and examined for S. mansoni infection using the Kato Katz technique. …”
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