Modified minimally invasive surgical technique plus Bio-Oss Collagen for regenerative therapy of isolated interdental intrabony defects: study protocol for a randomised controlled... by Ye Han, Churen Zhang, Haidong Zhang, Zhaoguo Yue, Lili Miao, Kaining Liu, Jianxia Hou

“…The patients will be randomly assigned to two groups, with 20 participants in each group, for further periodontal regenerative surgery. …”
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miRNA expression changes during the course of neoadjuvant bevacizumab and chemotherapy treatment in breast cancer by Evita Maria Lindholm, Miriam Ragle Aure, Mads Haugland Haugen, Kristine Kleivi Sahlberg, Vessela N. Kristensen, Daniel Nebdal, Anne‐Lise Børresen‐Dale, Ole Christian Lingjærde, Olav Engebraaten

“…Biopsies were sampled before treatment, after 12 weeks with anthracycline and after taxane therapy at week 25. …”
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Histological Alterations and Interferon-Gamma and AKT-mTOR Expression in an Experimental Model of Achilles Tendinopathy—A Comparison of Stem Cell and Amniotic Membrane Treatment by Guilherme Vieira Cavalcante, Rosangela Fedato, Lucia de Noronha, Seigo Nagashima, Ana Paula Camargo Martins, Márcia Olandoski, Ricardo Pinho, Aline Takejima, Rossana Simeoni, Julio Cesar Francisco, Luiz César Guarita-Souza

“…<b>Results:</b> There were no differences between morphometric histological when evaluating the Achilles tendons of the samples. No significant differences were found regarding the expression of AKT-2 and mTOR markers between the study groups. …”
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Single-Cell RNA Transcriptomics and Multi-omics Analyses Reveal the Clinical Effects of Acupuncture on Methadone Reduction by Yiming Chen, Baochao Fan, Jingchun Zeng, Yutian Zou, Chenyang Tao, Chen Chen, Peiming Zhang, Jian Liang, Fangfang Qi, Hailin Tang, Liming Lu

“…We collected peripheral blood mononuclear cells, plasma, and fecal samples from 48 MMT participants in a randomized, placebo-controlled trial. …”
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Comparison of hair steroid levels among Wistar rats exposed to different environmental enrichment settings by Alberto Elmi, Niccolò I. Vannetti, Viola Galligioni, Nadia Govoni, Camilla Aniballi, José M. Sánchez-Morgado, Maria L. Bacci, Domenico Ventrella

“…The rats were housed in ventilated cages with poplar bedding and nesting material. Hair was sampled again after 3 months (T1). Steroids were quantified using radioimmunoassay or enzyme-linked immunosorbent assay after methanol extraction. …”
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Effect of single versus multistrain probiotic in extremely preterm infants: a randomised trial by Gayatri Athalye-Jape, Karen Simmer, Sanjay Patole, Liwei Chen, Shripada Rao, Lakshmi Chandrasekaran, Dorota Doherty, Meera Esvaran, Elizabeth Nathan, Anthony Keil, Chooi Kok, Stephan Schuster, Patricia Conway

“…Faecal propionate (SS, p&lt;0.001, and TS, p=0.0009) and butyrate levels (TS, p=0.029) were significantly raised in T2 versus T1 samples. Secondary clinical outcomes were comparable. …”
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Systemic immunomodulation by irreversible electroporation versus stereotactic ablative body radiotherapy in locally advanced pancreatic cancer: the CROSSFIRE trial by Danielle Vos, Anita Stam, Tanja de Gruijl, Petrousjka van den Tol, Sinead Lougheed, Joyce Bakker, Bart Geboers, Florentine Timmer, Hester Scheffer, Alette Ruarus, Laurien Vroomen, Evelien Schouten, Robbert Puijk, Frank Lagerwaard, Jan de Vries, Anna Bruynzeel, Martijn Meijerink

“…We aimed to compare systemic immune responses between the treatments.Methods As part of the randomized phase II CROSSFIRE trial (NCT02791503), comparing the oncological efficacy of IRE to SABR in patients with LAPC, pre- and post-treatment (2 weeks and 3 months) peripheral blood samples were collected. …”
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Urinary and oral microbiota in Polish women: a pilot case-control study of breast cancer by Anna Marciniak, Marzena Skrzypczak-Zielińska, Oliwia Zakerska-Banaszak, Elżbieta Nowakowska, Anna Kozaczka, Brunon Zemła, Andrzej Szpak, Dariusz Godlewski, Jadwiga Charzewska, Dorothy R. Pathak

“…This study aims to investigate the composition of the urinary and oral microbiota in Polish breast cancer (BC) patients relative to healthy controls (HCs) and to predict relevant metabolic pathways of microbiota in studied groups.MethodsUrine and oral samples from 48 participants, 24 BC cases and 24 HCs, randomly selected from 417 BC cases and 514 HCs, were analyzed using next-generation sequencing of bacterial 16S rRNA gene (V1-V9) and fungal ITS regions, along with bioinformatics tools to identify and compare microbial communities and predict relevant pathways of microbiota in the studied groups.ResultsBC case urine microbiota contained an increased abundance of Corynebacterium (5.2-fold, but not significant) and Gammaproteobacteria including unknown genus and Pseudomonas (1.7- and 1.8-fold) and decreased abundance of Family XI (0.3-fold) and Bifidobacteriaceae (0.4-fold) compared to HCs. …”
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Initiating Empagliflozin and Sacubitril/Valsartan Early After Acute Myocardial Infarction: Mechanistic Study by Daina Martínez‐Falguera, Júlia Aranyó, Gemma Ferrer‐Curriu, Albert Teis, Elena Revuelta‐Lopez, Idoia Diaz‐Güemes, Marta Monguió‐Tortajada, Edgar Fadeuilhe, Oriol Rodríguez‐Leor, Francesc Poblador, Borja Montejo, Santiago Roura, Roger Villuendas, Axel Sarrias, Victor Bazan, Esther Jorge, Victoria Delgado, Francisco Rafael Jimenez‐Trinidad, Montserrat Rigol, Neus Martinez‐Micaelo, Núria Amigó, Antoni Bayes‐Genis, Felipe Bisbal, Carolina Gálvez‐Montón

“…At 30 days after MI, programmed electrical stimulation and high‐density mapping were performed and VT inducibility was assessed. Tissue samples were collected for cardiac inflammation, oxidative stress, and metabolic analyses. …”
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Therapeutic efficacy of Centratherum anthelminticum in subclinical mastitis: A biochemical and hematological assessment in lactating cattle by Muhammad Adil, Farrah Deeba, Muhammad Tariq, Muhammad Usman, Saba Saeed, Eliana Ibáñez-Arancibia, Patricio De los Ríos-Escalante, Muhammad Safdar

“…Milk samples were analyzed pre- and post-treatment for somatic cell count (SCC), pH, electrical conductivity, fat, protein, lactose, and solids-not-fat (SNF) content. …”
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Comparison of the Nutritional, Physicochemical, Technological–Functional, and Structural Properties and Antioxidant Compounds of Corn Kernel Flours from Native Mexican Maize Cultiv... by Luis Alfonso Hernández-Villaseñor, Salvador Hernández-Estrada, Víctor Manuel Gómez-Rodríguez, Humberto Ramírez-Vega, Zuamí Villagrán, Araceli Ortega-Martínez, Efigenia Montalvo-González, José Martín Ruvalcaba-Gómez, Napoleón González-Silva, Luis Miguel Anaya-Esparza

“…Enough cobs for each maize accession were randomly selected to yield 1000 g of corn kernels. …”
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Dynamic circulating tumor DNA indicates pathological benefits of additional neoadjuvant chemoimmunotherapy courses for locally advanced non-small-cell lung cancer patients by Dong Lin, Liang Wu, Pei Wang, Xiaolong Li, Xiaolan Wang, Yiran Cai, Kangli Xiong, Xi Chen, Fu Yang, Wei Huang, Xing Wang, Jiang Fan

“…The patients with IIIA NSCLC without driver genes were given two cycles of NCI (initial two-cycle NCI), after which they were 1:1 randomly assigned to the two-cycle NCI groups (no additional NCI) or four-cycle NCI group (with another two cycles of NCI).ResultsThis study involved 13 patients with 28 blood samples. …”
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Evaluation of Feed Grade Wheat Levels on Performance, Some Blood Parameters, Carcass Traits and Intestine Morphology in Male and Female Growing Japanese Quail by Zahra Asgari, Iman Hajkhodadadi, Hosseinali Ghasemi, Mahdi Khodaei Motlagh

“…Methods: In order to conduct this experiment, 560 one-day-old quail chicks were used in a completely randomized design with 7 treatments and 4 replications and 20 chicks per replication. …”
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Vitamin K2 Alleviates Insulin Resistance Associated Skeletal Muscle Atrophy via the AKT/mTOR Signalling Pathway by Yingfeng Zhang, Yina Wang, Zhu Ming, Bin Li, Haitao Qi, Hongquan Xie, Guoliang Wang, Jiepeng Chen, Lili Duan, Ran Li, Ying Li

“…Animals were sacrificed, and skeletal muscle and serum samples were collected to analyse muscle atrophy, glucose and lipid levels. …”
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Effects of peripartal rumen-derived direct-fed microbial supplementation on lactation performance, metabolism, ruminal fermentation, and microbial abundance in dairy cows by M. Bulnes, J. Bonilla, M. Suazo, T.C. Michelotti, A. Paz, J. Lefler, C. Marotz, M. Embree, G. Begalli, J. Halfen, T. Fernandes, E. Trevisi, M.E. Uddin, J.S. Osorio

“…Fifty-six Holstein cows were enrolled in a randomized complete block design from −21 to 100 DIM. …”
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Effect of Autolyzed Yeast on Performance and Physiological Indices of Broiler Chickens Reared at High Stock Density by Samaneh Ghaseminejad, Smayyeh Salari

“…Microbial populations for E. coli and Coliforms were counted after aerobic incubation at 37°C for 24 hours. All samples were plated in duplicate. The obtained data were statistically analyzed using SAS statistical software, GLM procedure. …”
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Immunoglobulin G absorption dynamics in neonatal calves: An investigation of the effects of casein presence in colostrum by E.V. Lopez-Bondarchuk, J.M.V. Pereira, G. Mazon, J.V.R. Lovatti, A.J. Geiger, J.H.C. Costa

“…ABSTRACT: Casein presence in colostrum may affect the absorption of immunoglobulins in neonate dairy calves. This randomized controlled trial investigated the effect of casein presence in colostrum replacer on serum IgG dynamics on calves. …”
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Farm-scale soil spatial variability at a mountain research centre in Northwestern Himalayas by Robina Kousar, Aabid H. Lone, Zahoor A. Shah, Eajaz Ahmad Dar, Mohd Salim Mir, Lamya Ahmed Alkeridis, Laila A. Al-Shuraym, Mumtaz A. Ganie, Javid. A. Bhat, Mohammed Ali Alshehri, Samy Sayed, Mustafa Shukry, Owais. A. Wani, Intikhab. A. Jehangir, Faheem. J. Wani, Zahoor. A. Baba, Najeeb. R. Sofi, T. Mubarak, Ashaq Hussain, Baby Summuna

“…The study area at 75.0916°E and 33.7237°N was the Research Farm of Mountain Research Centre for Field Crops (MRCFC) – Khudwani, characterized by temperate climate conditions. Soil samples were randomly collected from eighty-nine (89) sites across the whole research farm at a depth of 0–15 cm using a global positioning system (GPS). …”
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Surveying the Distribution and Diversity of Predominant Mycobiota in Cultivated and Uncultivated Soils of Zaria, Northwestern Nigeria by Nafisat Aliyu, Maryam L Faragai, Adamu Ibrahim, Karimat Aliyu, M D Ibrahim, Saratu Hussaini Mahmud

“…After removing the surface soil, soil samples were randomly collected from two locations at 0-15 cm depths. …”
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Effect of high intakes of protein-only and carbohydrate-only on plasma metabolites and hormones, in addition to nitrogen excretion by Matthieu Clauss, Claire Puissant, Nasser Ezzatkhah Bastani, Lotte L. K. Nielsen, Bjørn Steen Skålhegg, Per Bendix Jeppesen, Jørgen Jensen

“…They recorded their dietary intake the day before and during the intervention to repeat them across the second trial day. Blood samples were collected at regular intervals after drink ingestion. …”
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