Flexibility in PAM recognition expands DNA targeting in xCas9 by Kazi A Hossain, Lukasz Nierzwicki, Modesto Orozco, Jacek Czub, Giulia Palermo

“…Here, we elucidate the molecular mechanism underlying xCas9’s expanded PAM recognition and provide critical insights for expanding DNA targeting. …”
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Introduced substrates trigger colonization by reef-associated fish in a degraded coastal system. by Maryann S Watson, Jon Dickson, Oscar Franken, Laura L Govers, Tjisse van der Heide, Sterre Witte, Britas Klemens Eriksson

“…Coastal reefs benefit the survival and growth of mobile organisms by providing shelter and increased food availability. Under increasing pressure from human activities, the coverage of subtidal reefs has decreased along the world's coasts. …”
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Effect of plasma assisted combustion on emissions of a premixed nitrous oxide fuel blend by Boj N. Villanueva, Po-Hung Lin, Yueh-Heng Li, Jaime P. Honra

“…Chemiluminescence imaging was used to observe the changes in radical species emissions for a laminar premixed CH4/N2O/Ar flame under plasma actuation and emission results were measured with a flue gas analyzer. …”
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Betaine enhances SCAPs chondrogenic differentiation and promotes cartilage repair in TMJOA through WDR81 by Meiyue Wang, Zejie Wu, Xiaoyu Zheng, Yishu Huang, Yizhou Jin, Jiaxin Song, Wanzhen Lei, Hua Liu, Riyue Yu, Haoqing Yang, Runtao Gao

“…Here, we explored the effects and underlying mechanisms of betaine on chondrogenic differentiation of SCAPs. …”
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Enhancing magnetic hyperthermia: Investigating iron oxide nanoparticle coating and stability by Joana Santos, Jorge Carvalho Silva, Manuel A. Valente, Tânia Vieira, Paula I.P. Soares

“…This work explores diverse SPIONs coating options - oleic acid (OA), dimercaptosuccinic acid (DMSA), and (3-aminopropyl)triethoxysilane (APTES), to improve SPIONS stability under storage while keeping their heating capacity. …”
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Obtention of stable neuroprotective extracts from orange by-products by gas-expanded liquids extraction (GXLE) and liposomal microencapsulation by Victor M. Amador-Luna, Lidia Montero, Pablo Mendoza-Orbaneja, Elena Ibáñez, Miguel Herrero

“…Consequently, the overall bioactivity of the encapsulated product was reduced due to this value, although the product was demonstrated to be stable for >4 months of storage under accelerated conditions (45 °C and 80 % humidity), while the non-encapsulated original extract lost 50 % of its activity in less than a month. …”
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Impact of climate change on the distribution of Isaria cicadaeMiquel in China: predictions based on the MaxEnt model by Zhipeng He, Habib Ali, Junhao Wu, Zhiqian Liu, Xinju Wei, Zhihang Zhuo

“…Understanding its potential distribution is essential for promoting sustainable harvesting practices.MethodsThis study utilizes the MaxEnt model, combined with known distribution records and 22 environmental variables, to predict the potential distribution of I. cicadae under three representative emission scenarios (CMIP6: SSP1-2.6, SSP2-4.5, and SSP5-8.5) for the 2050s and 2070s.ResultsThe analysis identifies seven key environmental variables influencing the habitat suitability of I. cicadae: the mean temperature of the driest quarter (bio09), the mean temperature of the wettest quarter (bio08), precipitation in the wettest month (bio16), the mean diurnal range (bio02), isothermality (bio03), elevation, and slope. …”
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Ammonifying and phosphorus-solubilizing function of Aliikangiella maris sp. nov. isolated from Phaeocystis globosa bloom and algal–bacterial interactions by Fei Li, Fei Li, Ming-Ben Xu, Liang-Hao Pan, Jie Li, Cai-Bi Lan, Zhe Li, Shan Lu, Jun-Xiang Lai, Jun-Xiang Lai, Peng-Fu Li

“…Co-culture experiments showed that strain GXAS 306T significantly improved algal growth parameters of two typical P. globosa strains (Pg293 and PgV01), particularly under nitrogen or phosphorus deficiency. Specifically, cell densities were observed to increase by 19.6–86.0%, accompanied by substantial enhancements in photosynthetic performance with increases of 8.0–30.6% in Fv/Fm and 10.9–27.9% in rETRmax. …”
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Effects of Ni content on the microstructure and properties of laser powder bed fusion-processed Fe–18Cr–XNi alloys by Yongchao Lu, Xiaoqun Li, Yazhou He, Yaqing Hou, Fafa Li, Yao Lu, Hao Zhang, Lanting Zhang, Hong Wang, Hang Su

“…This study investigates the evolution of microstructure and performance in Fe–18Cr–XNi alloys under identical LPBF processing conditions as a function of Ni content. …”
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Gut microbiota derived DCA enhances FOLFOX efficacy via Ugt1a6b mediated enterohepatic circulation in colon cancer by Qian Fang, Xiaoying Hou, Limei Fan, Yufei Deng, Xiaoxuan Li, Hongyun Zhang, Haiping Wang, Zhengqi Fu, Binlian Sun, Xiji Shu, Hongzhi Du, Yuchen Liu

“…Our previous studies have suggested that gut microbiota-derived bile acids may be involved in the anticancer effect of FOLFOX in vitro, however, the underlying mechanism remains unclear. In this study, we investigated the role of bile acids in modulating FOLFOX efficacy and the related mechanisms. …”
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Force analysis of slope reinforced by Frame-Anchor-Support-Structure based on improved horizontal slice method. by Guang-Wen Fang, Fei Guo, An-Ping Huang

“…By comparing and analyzing with the overall sliding wedge method, the reasonableness and accuracy of the calculation method of this paper are verified, and the variety rule of force on the slope reinforced by FASS under different loads is obtained. The results show that the combined force of lateral earth pressures on slopes calculated in this paper is smaller than that of the overall sliding wedge method, but the difference between the two is not significant (within 9% difference). …”
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Effects of infliximab biosimilar on retinal microvasculature in Crohn’s disease patients evaluated by optical coherence tomography angiography by Hesham M. El Mazar, El sayed E. El Shayeb, Asmaa M. Ebrahim, Ayatullah S.A. Motawe, Esraa S. El-Ghoubashy

“…Objective To compare the retinal microcirculation in Crohn’s disease cases under infliximab (IFX) biosimilar with controls that are age- and sex-matched in terms of disease severity. …”
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MAEMC-NET: a hybrid self-supervised learning method for predicting the malignancy of solitary pulmonary nodules from CT images by Tianhu Zhao, Tianhu Zhao, Yong Yue, Hang Sun, Jingxu Li, Yanhua Wen, Yudong Yao, Wei Qian, Yubao Guan, Shouliang Qi, Shouliang Qi

“…A total of 494 patients from two centers were included.ResultsMAEMC-NET achieved an area under curve (95% Confidence Interval) of 0.962 (0.934–0.973). …”
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Exogenous nitric oxide relieves iron-inhibitory effect on wheat seed germination by Shuqian Shi, Zhe Zhang, Rongrong Ma, Yihui Tao, Ziling Wang, Yingli Yang

“…Considering that nitric oxide (NO) has been shown to ameliorate the negative effects of abiotic stress in several plants, we investigated the effect and mechanisms of exogenous NO in wheat seed germination under Fe stress, focusing on oxidative stress. Specifically, seeds of the wheat cultivar ‘Ningchun 4’ were treated with 1 mM FeCl3 (Fe) or with the NO donor sodium nitroprusside (SNP) and/or the specific NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO). …”
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Biophysical modeling and experimental analysis of the dynamics of C. elegans body-wall muscle cells. by Xuexing Du, Jennifer Crodelle, Victor James Barranca, Songting Li, Yunzhu Shi, Shangbang Gao, Douglas Zhou

“…To ensure the model accurately reflects cellular responses under depolarizing currents, we develop a parallel simulation-based inference method for determining the model's free parameters. …”
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Membrana preformativa: Unveiling the unexplored facets of dental development by Sakshi Sikaria, Suganya Panneer Selvam, Ramya Ramadoss, Sandhya Sundar

“…Through rigorous analysis, we seek to elucidate the intricate mechanisms underlying tooth development, helping to advance our understanding of this vital biological process. …”
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PROSPECTS AND CHALLENGES OF RESCUING THE SHRINKING LAKE CHAD THROUGH THE UNITED NATIONS’ GREEN CLIMATE FUND by DAIYABU HASSAN MUHAMMAD

“…Although there is a new initiative by the Italian government and the Chinese construction company PowerChina in partnership with Bonfica to resurrect the idea, the paper argues that the LCBC has failed to access and utilize the adaptation facilities under the United Nations adaptation funds. Drawing from the argument of the justice for international adaptation framework, the paper assesses the vulnerabilities and opportunities of the Lake Chad countries and conclude that, to bring the IBWT to fruition, the LCBC and the riparian states of the Lake Chad must explore and access all available sources of fund, especially the Green Climate Fund. …”
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Airborne Ultrafine Particle and Acute Physiological Effects during Maximal Aerobic Power Test by Angelo Rodio, Francesco Misiti, Alessandro Zagaglia, Luca Stabile, Giorgio Buonanno, Luigi Fattorini

“…On the contrary, VO2peak increased (P < 0.05) under high exposure (41.6 ± 4.31 mL kg–1 min–1), during high-intensity exercise, compared to a low exposure (38.4 ± 4.05 mL kg–1 min–1). …”
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Isolated Idiopathic Cervical Dystonia with a Rare Type of Atlantoaxial Dislocation. A Case Report and a Short Literature Review by Aldakheel DA

“…Despite initial attempt of close reduction under anesthesia, recurrence of the subluxation occurred, and surgical intervention was necessary.Conclusion: This case underscores the importance of thorough clinical evaluation and imaging in the diagnosis of isolated cervical dystonia, particularly given its potential to present with atypical features. …”
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Divergence of alpine plant populations of three Gentianaceae species in the Qinling sky Island by Peng-Cheng Fu, Bing-Jie Mo, He-Xin Wan, Shu-Wen Yang, Rui Xing, Shan-Shan Sun

“…Divergence time estimation based on plastomes and approximate Bayesian computation based on genomic SNPs showed that Qinling populations of the three Gentianaceae species originated at different periods under various patterns including primary source and hybridization. …”
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