Alteration of microbiota antibody‐mediated immune selection contributes to dysbiosis in inflammatory bowel diseases by Eva Michaud, Louis Waeckel, Rémi Gayet, Roman Goguyer‐Deschaumes, Blandine Chanut, Fabienne Jospin, Katell Bathany, Magali Monnoye, Coraline Genet, Amelie Prier, Caroline Tokarski, Philippe Gérard, Xavier Roblin, Nicolas Rochereau, Stéphane Paul

“…Here, we asked whether IgA1 and IgA2‐microbiota interactions might be involved in dysbiosis induction during inflammatory bowel diseases. Using stool HPLC‐purified IgA, we show that reverse‐transcytosis is abrogated in ulcerative colitis (UC) while it is extended to IgA1 in Crohn's disease (CD). 16S RNA sequencing of IgA‐bound microbiota in CD and UC showed distinct IgA1‐ and IgA2‐associated microbiota; the IgA1+ fraction of CD microbiota was notably enriched in beneficial commensals. …”
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Advantages of a genomic DNA-based next-generation sequencing assay for detection of mutant NPM1 measurable residual disease in AML by Christian M. Vonk, Tim Grob, Melissa Rijken, François G. Kavelaars, Jolinda M. L. Konijnenburg, Gert J. Ossenkoppele, Markus G. Manz, Violaine Havelange, Yngvar Fløisand, Bob Löwenberg, Peter J. M. Valk

“…Because of recent advancements of promising drugs targeting mNPM1 AML, monitoring of mNPM1 MRD has gained interest, and is generally done by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). …”
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Therapeutic Approaches with Iron Oxide Nanoparticles to Induce Ferroptosis and Overcome Radioresistance in Cancers by Dorianne Sant’Angelo, Géraldine Descamps, Valentin Lecomte, Dimitri Stanicki, Sébastien Penninckx, Tatiana Dragan, Dirk Van Gestel, Sophie Laurent, Fabrice Journe

“…Moreover, IONPs, by releasing iron ions, can induce ferroptosis, a form of iron-dependent cell death, which offers a promising pathway to reverse radio- and chemoresistance in cancer therapy. …”
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MicroRNA Hsa-Let-7b Regulates the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting CTHRC1 by Lin Fu, Na Li, Yu Ye, Xiaying Ye, Tong Xiao, Xiao Wu, Yanxia Ma, Jinhua Yu

“…On the other hand, the osteogenic differentiation capacity was detected by alkaline phosphatase (ALP) staining and activity, alizarin red staining, Western blot, and quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR). …”
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In Silico Designed Multi-Epitope Vaccine Based on the Conserved Fragments in Viral Proteins for Broad-Spectrum Protection Against Porcine Reproductive and Respiratory Syndrome Viru... by Shaukat Ullah, Hikmat Ullah, Kainat Fatima, Tan Lei

“…Furthermore, systematic evaluations of their physicochemical properties, structural stability, binding with pattern recognition receptors, and induction of the host immune system were performed. …”
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Itraconazole inhibits the Wnt/β-catenin signaling pathway to induce tumor-associated macrophages polarization to enhance the efficacy of immunotherapy in endometrial cancer by Xin Guan, Xin Guan, Lu Han, Lu Han

“…Western blotting and Real-time reverse transcription polymerase chain reaction (RT-PCR) were used to investigate the impact of ITZ on the Wnt/β-catenin signaling pathway. …”
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Role of CD133 antibody-conjugated nanocarrier in enhancing the targetability of hepatocellular carcinoma stem cells by Hadeer A. Aglan, Ahmed A. Abd-Rabou, Hanaa H. Ahmed, Ghada H. Elsayed, Mohamed S. Kishta, Manar A. Elhinnawi, Nadia S. Mahmoud

“…The flow cytometric analysis revealed that Q NPs recorded the highest induction of apoptosis (77.8%) relative to the control (1.8%). …”
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Transplantation of Goat Bone Marrow Mesenchymal Stem Cells (gMSCs) Help Restore Spermatogenesis in Endogenous Germ Cells-Depleted Mouse Models by Fang WANG, Chao LIU, Shan-shan ZHANG, Wei-shuai LIU, Jin-lian HUA

“…The results showed that characteristic gMSC lines were established and a small population of gMSCs transdifferentiated into male germ cell-like cells which expressed Stra8 after induction with retinoic acid (RA), as analysed by reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence. …”
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LncRNA NEAT1 sponges miR-214-3p to promote osteoblast differentiation through regulating the PI3K/AKT/mTOR pathway in aortic valve calcification by Rongjian Xu, Yuhui Sun, Fangfei Tian, Min Zhao

“…Importantly, NEAT1 could directly interact with miR-214-3p and change of miR-214-3p expression could efficiently reverse PTEN expression and osteogenic differentiation induced by NEAT1. …”
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Spontaneously Formed Spheroids from Mouse Compact Bone-Derived Cells Retain Highly Potent Stem Cells with Enhanced Differentiation Capability by Kai Chen, Xianqi Li, Ni Li, Hongwei Dong, Yiming Zhang, Michiko Yoshizawa, Hideaki Kagami

“…The expression levels of embryonic stem cell markers were analyzed using immunofluorescence and quantitative reverse transcription polymerase chain reaction. Then, the cells from spheroids were induced into osteogenic and neurogenic lineages. …”
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Effect of irinotecan on HMGB1, MMP9 expression, cell cycle, and cell growth in breast cancer (MCF-7) cells by Saeedeh Keyvani-Ghamsari, Azra Rabbani-Chadegani, Javad Sargolzaei, Maryam Shahhoseini

“…Irinotecan decreased H3K9 acetylation and increased poly ADP-ribose polymerase fragmentation to 89 kDa and anion superoxide production suggesting induction of apoptosis in these cells. Propidium iodide staining of the cells 24 h after the drug treatment revealed arrest of the cells in S-phase. …”
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Pain mediator NGF improves chondrocyte extracellular matrix synthesis via PI3K/AKT pathway by Mengling Wang, Jie Lian, Maoqing Ye, Bingchen An

“…Further analysis indicated that GNF5837 (TRKA inhibitor) or LY294002 (PI3K inhibitor) could reverse the protective effects of NGF on chondrocyte matrix synthesis. …”
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Requirement of heterogeneous nuclear ribonucleoprotein C for BRCA gene expression and homologous recombination. by Rachel W Anantha, Allen L Alcivar, Jianglin Ma, Hong Cai, Srilatha Simhadri, Jernej Ule, Julian König, Bing Xia

“…Depletion of hnRNP C substantially altered the normal balance of repair mechanisms following DSB induction, reducing HR usage in particular, and impaired S phase progression after IR. …”
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HNF1α Controls Liver Lipid Metabolism and Insulin Resistance via Negatively Regulating the SOCS-3-STAT3 Signaling Pathway by Jiaorong Tan, Jiahong Xu, Guohua Wei, Lijuan Zhang, Long’e Sun, Guangyu Wang, Fei Li, Fengxiang Jiang

“…Inhibitors of STAT3 can reverse the regulation of decreased HNF1α expression on the insulin signaling pathway and fat metabolism. …”
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The diagnostic and therapeutic potential of miR-3149 in osteoporotic fractures through SMAD4 by Guanwei Xu, Zhichao Yang, Sibao Zeng, Yongqi Dong, Lina Huang

“…Co-transfection with pcDNA-SMAD4 reversed the effects of mimic-miR on RAW264.7 cell proliferation, apoptosis, and osteoclastic induction. …”
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Effects of Dimethylaminoethanol and Compound Amino Acid on D-Galactose Induced Skin Aging Model of Rat by Su Liu, Zhenyu Chen, Xia Cai, Ying Sun, Cailing Zhao, Fangjun Liu, Dalie Liu

“…A lasting dream of human beings is to reverse or postpone aging. In this study, dimethylaminoethanol (DMAE) and compound amino acid (AA) in Mesotherapy were investigated for their potential antiaging effects on D-galactose induced aging skin. …”
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The Association of OLFM4 with the Progression and Cisplatin Resistance of Head and Neck Squamous Carcinoma by Xinlu He, Xi Yao, Keling Pang, Xulin Chen, Zhengbo Wei, Ying Xie

“…These findings suggest OLFM4 may serve as a prognostic biomarker for HNSCC and a potential therapeutic target to reverse cisplatin resistance in HNSCC.…”
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Inhibition of PRC1 elicits immunogenic cell death by triggering ROS-dependent ER stress in colorectal cancer via the Wnt/β-catenin signaling pathway by Wei Wang, Lijiang Zhou, Xinyu Zhang, Zheng Li

“…According to results of rescue assays, activation of Wnt/β-catenin by BML-284 could partially reverse the effects of PRC1 knockdown on ER stress and ICD in CRC cells. …”
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Minimizing nocebo effects by conditioning with verbal suggestion: A randomized clinical trial in healthy humans. by Danielle J P Bartels, Antoinette I M van Laarhoven, Michiel Stroo, Kim Hijne, Kaya J Peerdeman, A Rogier T Donders, Peter C M van de Kerkhof, Andrea W M Evers

“…First, negative expectations about electrical itch stimuli were induced by verbal suggestion and conditioning (part 1: induction of nocebo effect). Second, participants were randomized to either the experimental group or one of the control groups (part 2: reversing nocebo effect). …”
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Photosynthetic response of Solidago gigantea Aition and Calamagrostis epigejos L. (Roth) to complex environmental stress on heavy metal contaminated sites by Wojciech Bąba, Agnieszka Kompała-Bąba, Edyta Sierka, Wojciech Bierza

“…The increase in the I–P part of the induction curve typically attributed to the reduction of electron transporters (ferredoxin, intermediary acceptors, and NADP) of the PSI acceptor side was observed in both SgC and SgLZ but not in CeLZ populations. …”
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