Parylene-C Modified OSTE Molds for PDMS Microfluidic Chip Fabrication and Applications in Plasma Separation and Polymorphic Crystallization by Muyang Zhang, Haonan Li, Xionghui Li, Zitong Ye, Qinghao He, Jie Zhou, Jiahua Zhong, Hao Chen, Xinyi Chen, Yixi Shi, Huiru Zhang, Lok Ting Chu, Weijin Guo

“…This work presents a novel microfabrication process that addresses the interference of thiol groups on off-stoichiometry thiolene (OSTE) surfaces with the curing of polydimethylsiloxane (PDMS) by integrating the high-performance polymer Parylene-C. …”
Get full text

Photoreceptor Differentiation following Transplantation of Allogeneic Retinal Progenitor Cells to the Dystrophic Rhodopsin Pro347Leu Transgenic Pig by H. Klassen, J. F. Kiilgaard, K. Warfvinge, M. S. Samuel, R. S. Prather, F. Wong, R. M. Petters, M. la Cour, M. J. Young

“…Transplantation of stem, progenitor, or precursor cells has resulted in photoreceptor replacement and evidence of functional efficacy in rodent models of retinal degeneration. Ongoing work has been directed toward the replication of these results in a large animal model, namely, the pig. …”
Get full text

Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia. by Astanand Jugessur, Min Shi, Håkon Kristian Gjessing, Rolv Terje Lie, Allen James Wilcox, Clarice Ring Weinberg, Kaare Christensen, Abee Lowman Boyles, Sandra Daack-Hirsch, Truc Trung Nguyen, Lene Christiansen, Andrew Carl Lidral, Jeffrey Clark Murray

“…<h4>Background</h4>Fetal conditions can in principle be affected by the mother's genotype working through the prenatal environment.<h4>Methodology/principal findings</h4>Genotypes for 1536 SNPs in 357 cleft candidate genes were available from a previous analysis in which we focused on fetal gene effects. …”
Get full text

The Zwicker tone as a model to investigate auditory processing and tinnitus: a scoping review by Jude L. R. Barker, Derek J. Hoare, Derek J. Hoare, Derek J. Hoare, Magdalena Sereda, Magdalena Sereda, Joseph Sollini

“…Several methodological issues potentially affecting responder rate were identified, however, which warrants replication and extension, with careful control. We conclude that (1) Zwicker tone perception relies on a range of factors both stimulus and cognitive related, (2) Further work is required to map the parameters that induce the Zwicker tone and (3) While obvious similarities exist linking tinnitus and Zwicker tones more work is needed to prove the link between the two.…”
Get full text

Metabolic adaptability and nutrient scavenging in Toxoplasma gondii: insights from ingestion pathway-deficient mutants by Patrick A. Rimple, Einar B. Olafsson, Benedikt M. Markus, Fengrong Wang, Leonardo Augusto, Sebastian Lourido, Vern B. Carruthers

“…Our study suggests that the ingestion pathway contributes to sustaining parasite metabolites and parasite replication under amino acid-limiting conditions. This work advances our understanding of the metabolic adaptability of Toxoplasma.…”
Get full text

A GWAS of angiotensin-converting enzyme inhibitor-induced angioedema in a South African population by Jacquiline W. Mugo, PhD, Cascia Day, Cert Allergy (Phys), Ananyo Choudhury, PhD, Maria Deetlefs, Dip Allergy (SA), Robert Freercks, MPhil, Sian Geraty, MMed, Angelica Panieri, MBChB, Christian Cotchobos, MBChB, Melissa Ribeiro, MBChB, Adelein Engelbrecht, MMed, Lisa K. Micklesfield, PhD, Michèle Ramsay, PhD, Sarah Pedretti, PhD, Jonny Peter, PhD

“…Conclusions: Our results highlight the importance of African populations for detection of novel variants in replication studies. Further increased sampling across the continent and matched functional work are needed to confirm the importance of genetic variation in understanding the biology of AE-ACEI.…”
Get full text

Analysis of the association of influenza clinical course with single nucleotide polymorphisms in genes affecting the interferon-λ3 production by Lyudmila I. Nikolaeva, Maya D. Stuchinskaya, Kristina P. Telepenina, Nadezhda G. Shevchenko, Victor V. Kuprianov, Kirill G. Krasnoslobodtsev, Evgenya A. Mukasheva, Svetlana V. Trushakova, Irina N. Khlopova, Irina S. Kruzhkova, Lidya B. Kisteneva, Lyudmila V. Kolobukhina, Elena I. Burtseva

“…In the early stages of influenza infection such components of innate immunity as interferons I (α/β) and III (λ) type play a significant role in limiting virus replication. The aim of the work was to investigate associations of single nucleotide polymorphism in IFNL3 (rs8099917 T/G) and IFNL4 (rs12979860 C/T) genes with different course of influenza, and identify genetic markers of influenza complicated by community-acquired pneumonia. …”
Get full text

Evolution of Mechanization and Automation in Dairy Farming by Yu. A. Tsoy, V. V. Kirsanov, R. A. Mamedova, S. V. Kirsanov

“…A significant milestone in the advancement of the theory and practice of animal husbandry processes was the pioneering work in developing and implementing technology for flow-conveyor animal service, headed by Member of the Russian Academy of Sciences L.P. …”
Get full text

Polarization Multiplexing Dammann Grating Based on All-Dielectric Metasurface by Chenyang Wu, Xuanlun Huang, Yipeng Ji, Jiaxing Wang, Connie J. Chang-Hasnain

“…In this study, we present a novel polarization multiplexing DGs employing an all-dielectric metasurface. Our work introduces a DG optimization algorithm tailored for specific desired array patterns, including non-centrally symmetric arrays and arrays maximizing specific desired orders. …”
Get full text

A Systems Biology Approach to Elucidating Functional and Genomic Signatures in Host-Pathogen Interactions of Triple Infections: Tuberculosis, HIV, and SARS-CoV-2 by Mohd. Imran, Hayat Ali Alzahrani, Ranya Mohammed Elmagzoub, Zia Ur Rehman, Khadiga G. Abd Elaleem, Ahlam S. Mohamed, Aida A. Elsharief, Hayaa M. Alhuthali

“…The convergence of HIV-1, Mycobacterium tuberculosis, and SARS-CoV-2 infections presents a formidable challenge characterized by heightened immune suppression and persistent viral replication. Understanding the shared molecular mechanisms underlying these co-infections is essential, providing a foundation for future translational research and therapeutic discovery. …”
Get full text

Identification of Benzothiophene-Derived Inhibitors of Flaviviruses by Targeting RNA-Dependent RNA Polymerase by Leah Liu Wang, Shazeed-Ul Karim, Aidan Hand, Ryan Brunkhorst, Mackenna Petersen, Sarah Altman, Yi Liu, Luwen Zhang, Fengwei Bai, Shi-Hua Xiang

“…To develop effective drugs for treating these viruses’ infections, we create a new approach for developing common or shared drugs that may work for several different viral species of flaviviruses. …”
Get full text

Sarcoendoplasmic reticulum calcium ATPase is an essential and druggable lipid-dependent ion pump in Toxoplasma gondii by Rosalba Cruz-Mirón, Namita Pandey, Dimitrios Alexandros Katelas, Arunakar Kuchipudi, Dharmarajan Sriram, Aditi Gangopadhyay, Soumyananda Chakraborti, Ratnesh Kumar Srivastav, Nishith Gupta

“…Calcium homeostasis is pivotal for its asexual reproduction in mammalian host cells, and sarcoendoplasmic reticulum calcium-ATPase (SERCA) is considered vital for maintaining ion homeostasis within the parasite. This work studied the physiological relevance, structure-function relationship, mechanism, and therapeutic value of SERCA in the acutely-infectious tachyzoite stage of T. gondii. …”
Get full text

Advances in the Drug Development and Quality Evaluation Principles of Oncolytic Herpes Simplex Virus by Basma Eid Abdullah Ghorab, Tongtan Liu, Min Ying, Ping Wang, Meirong Qin, Jiayong Xing, Huadong Wang, Fuqiang Xu

“…Oncolytic herpes simplex virus (oHSV) represents a promising therapeutic approach to treating cancers by virtue of its selective replication in and lysis of tumor cells, with stimulation of host antitumor immunity. …”
Get full text

The impact of a commercial lower extremity exoskeleton on metabolic load, perceived exertion, and physiological response to a challenging military relevant task: A randomized cross... by JoEllen M Sefton, Frances K Neal, Philip J Agostinelli, Nicholas C Bordonie, Phillip E Whitley, Nathan T Pickle, Paulien E Roos

“…There may also be physiological benefits such as reduced core temperate and heart rate. Replication of this work in the field environment is warranted.…”
Get full text

Patient and public involvement in healthcare: a systematic mapping review of systematic reviews – identification of current research and possible directions for future research by Maria-Jose Santana, Axel Wolf, Jana Bergholtz, Vanessa Crine, Helena Cleeve, Ida Björkman

Get full text

Candidate genes for productivity identified by genome-wide association study with indicators of class in the Russian meat merino sheep breed by A. Y. Krivoruchko, O. A. Yatsyk, E. Y. Safaryan

“…The listed genes encode proteins involved in the control of the cell cycle and DNA replication, regulation of cell proliferation and apoptosis, lipid and carbohydrate metabolism, the development of the inflammatory process and the work of circadian rhythms. …”
Get full text

Integrated Network Analysis Decipher ZNF384‐Related miR‐20b‐5p and miR‐424‐5p in Colon Adenocarcinoma by Bo Zhang, Yoshihisa Matsumoto

“…Gene functional analysis showed enrichment of genes in DNA repair, replication fork, and cell cycle checkpoint signaling pathways. …”
Get full text

Discovery of Quinazolone Pyridiniums as Potential Broad-Spectrum Antibacterial Agents by Jie Dai, Qianyue Li, Ziyi Li, Zhonglin Zang, Yan Luo, Chenghe Zhou

“…The overprescription of antibiotics in medicine and agriculture has accelerated the development and spread of antibiotic resistance in bacteria, which severely limits the arsenal available to clinicians for treating bacterial infections. This work discovered a new class of heteroarylcyanovinyl quinazolones and quinazolone pyridiniums to surmount the increasingly severe bacterial resistance. …”
Get full text

Describing the elements of an effective dementia palliative care service by Siobhan Fox, Jonathan Drennan, Suzanne Guerin, W. George Kernohan, Aileen Murphy, Niamh O’Connor, Suzanne Timmons

“…While some services exist, a lack of description and scant detail on how and why they work makes it difficult for others to learn from existing successful models and impedes replication. …”
Get full text

The Production of the Recombinant Protein of Anthrax Bacteriolysine PlyPH and In Vitro Study of the Lytic Properties of the Protein Coded for them Against Bacillus anhracis Microbi... by N. V. Onuchina, A. V. Kuznetsovskiy, V. D. Soybanov

“…The use of lytic enzymes of species-specific bacteriophages is a new trend in the diagnosis, prevention and treatment of infectious diseases. The goal of this work is the cloning of the anthrax bacteriolysin PlyPH gene as part of the pTrcHis2C vector in Escherichia coli and the in vitro study of the lytic properties of the protein encoded by it against B. anthracis microbial cells. …”
Get full text