How thoughtful experimental design can empower biologists in the omics era by Maggie R. Wagner, Manuel Kleiner

“…Abstract The modern biology toolbox continues to evolve, as cutting-edge molecular techniques complement some classic approaches and replace others. However, statistical literacy and experimental design remain critical to the success of any empirical research, regardless of which methods are used to collect data. …”
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An anthropomorphic phantom for atrial transseptal puncture simulation training by Aya Mutaz Zeidan, Zhouyang Xu, Lisa Leung, Calum Byrne, Sachin Sabu, Yijia Zhou, Christopher Aldo Rinaldi, John Whitaker, Steven E. Williams, Jonathan Behar, Aruna Arujuna, R. James Housden, Kawal Rhode

“…This study presents a novel, patient-specific, anthropomorphic phantom for TSP simulation training that can be used with X-ray fluoroscopy and ultrasound imaging. Methods The TSP phantom was developed using additive manufacturing techniques and features a replaceable fossa ovalis (FO) component to allow for multiple punctures without replacing the entire model. …”
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Effects of scrap rubber waste on the mechanical performance of mortar made of crushed sand and sediment by Adda Hadj Mostefa, Benamar Balegh, Hamid Sellaf, Mohamed Elamine Dahamni

“…The primary goal is to valorize crushed sand particles through physical and mechanical tests, evaluating their potential as an alternative to natural sand in mortar mixtures. Methods: Experimental work was carried out to study the impact of partially and fully replacing sediments with crushed sand particles in mortar mixes. …”
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Advancing Early Detection of Major Depressive Disorder Using Multisite Functional Magnetic Resonance Imaging Data: Comparative Analysis of AI Models by Masab Mansoor, Kashif Ansari

“…ObjectiveThis study aimed to develop and validate ML models using multisite functional magnetic resonance imaging data for the early detection of MDD, compare their performance, and evaluate their clinical applicability. MethodsWe used functional magnetic resonance imaging data from 1200 participants (600 with early-stage MDD and 600 healthy controls) across 3 public datasets. …”
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Pioneering innovations in biodegradable packaging for meat products: Exploring intelligent pH indicator films by Sana Mohammadi, Sajad Pirsa, Farzad Mirab toupchi

“…They achieve this by effectively controlling the permeability of gases, thus regulating the internal atmosphere within the packaging, and maintaining optimal humidity levels. …”
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Formation of AAV single stranded DNA genome from a circular plasmid in Saccharomyces cerevisiae. by Tiziana Cervelli, Ana Backovic, Alvaro Galli

“…When a plasmid containing a rAAV genome in which the cap gene was replaced with the S. cerevisiae URA3 gene, was co-transformed in yeast with a plasmid expressing Rep68, a significant number of URA3(+) clones were scored (more than 30-fold over controls). …”
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La gestion de l’eau à Bibracte (Saône-et-Loire), avant et après la Conquête romaine by Laetitia Borau

“…So far research has focused to only a limited extent on the control of water in Gallic hilltop settlements during the Iron Age. …”
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Reuse of peanut shells and Azolla mixes as a peat alternative in growth medium of Dieffenbachia amoena ‘tropic snow’ by Ali Mahboub Khomami, Mohammad Naghi Padasht, Ali Ajili Lahiji, Fariba Mahtab

“…The 30% compost treatment led to significant differences in the final height (32.06 cm), trunk diameter (11.66 mm), stem and leaf fresh weight (57.52 g), and stem and leaf dry weight (5.10 g) in comparison with the controls. Conclusions Considering the high price of peat in comparison with compost, replacing peat with 30% compost is economically preferable. …”
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Energy­Saving Lighting of Agricultural Premises and its Parameters Calculation by L. Yu. Yuferev

“…(Research purpose) The research purpose is to substantiate the parameters of energy-saving lighting for agricultural premises and to develop methods for its calculation. (Materials and methods)  It has been shown that greenhouse farms most often use high and low pressure sodium lamps for lighting, but their spectrum only partially substitutes the natural one. …”
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One-pot Golden Gate Assembly of an avian infectious bronchitis virus reverse genetics system. by Katharina Bilotti, Sarah Keep, Andrew P Sikkema, John M Pryor, James Kirk, Katalin Foldes, Nicole Doyle, Ge Wu, Graham Freimanis, Giulia Dowgier, Oluwapelumi Adeyemi, S Kasra Tabatabaei, Gregory J S Lohman, Erica Bickerton

“…We demonstrate the use of this system for generation of point mutants and gene replacements. This Golden Gate Assembly-based reverse genetics system will enable rapid response to emerging variants of IBV, particularly important to vaccine development for controlling spread within poultry populations.…”
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DMSO-free cryopreservation of hiPSC-derived cardiomyocytes: low temperature characterization and protocol development by Akshat S. Mallya, Tessa Burrows, Jeanne Hsieh, Troy Louwagie, James R. Dutton, Brenda M. Ogle, Allison Hubel

“…A differential evolution (DE) algorithm was utilized to determine the optimal composition of a mixture of a sugar, sugar alcohol and amino acid to replace DMSO as the CPA. The hiPSC-CMs were subjected to controlled-rate freezing at different cooling rates and nucleation temperatures. …”
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Automatic Vegetable Watering System Using Fuzzy Logic with Integration of Soil Moisture, Rain Sensors, and RTC by Dallarizki Arginanta, Wahid Miftahul Ashari

“…The system is designed to replace manual watering methods with an automated process, thus improving the efficiency and effectiveness of vegetable cultivation. …”
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Current Clinical Laboratory Challenges to Widespread Adoption of Phage Therapy in the United States by Ahnika Kline, Ana G. Cobián Güemes, Jennifer Yore, Chandrabali Ghose, Daria Van Tyne, Katrine Whiteson, David T. Pride

“…Current barriers include a lack of standard methodology and quality controls for phage susceptibility testing and selection, the absence of phage-antibiotic synergy testing, and the absence of standard methods to assay phage activity on biofilms. …”
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Leveraging large language models for automated detection of velopharyngeal dysfunction in patients with cleft palate by Myranda Uselton Shirk, Catherine Dang, Jaewoo Cho, Hanlin Chen, Lily Hofstetter, Jack Bijur, Claiborne Lucas, Andrew James, Ricardo-Torres Guzman, Andrea Hiller, Noah Alter, Amy Stone, Maria Powell, Matthew E. Pontell, Matthew E. Pontell

“…This study explores the application of OpenAI's Whisper model for automated hypernasality detection, offering a scalable and efficient alternative to traditional approaches.MethodsThe Whisper model was adapted for binary classification by replacing its sequence-to-sequence decoder with a custom classification head. …”
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Role of Artificial Intelligence in Dentistry by V. Hysenaj-Cakolli, B. Kolgeci, V. Hysenaj-Hoxha, D. Kolgeci

“…This study aims to review the literature on the use of AI in some fields of dentistry. Methods and Results: A comprehensive search was conducted on various electronic databases, including PubMed and Scopus, to find relevant articles written in the English language. …”
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The Anthropological Factor of the New Generation of War Technologies by A. A. Kovalev

“…Physical violence in the conditions of their activation is replaced by nonphysical violence using coercion, inducement and manipulation. …”
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Investigating muscle protein synthesis using deuterium oxide: The impact of dietary protein interventions across the lifespan by Matthew S. Brook

“…Much of our current understanding of protein intake, specifically its quantity, quality and distribution, comes from stable isotope‐labelled amino acid methods. These techniques, however, are limited by time constraints and controlled settings, providing only brief snapshots of MPS dynamics. …”
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Protocol for EHS Rapid Guideline: Systematic Review, Meta-Analysis, GRADE Assessment, and European Recommendations on Parastomal Hernia Prevention by Stavros A. Antoniou, Stavros A. Antoniou, Cesare Stabilini, Ourania Koutsiouroumpa, Dimitrios Mavridis, Dimitrios Mavridis, Filip Muysoms

“…Previous guidelines recommend using a prophylactic mesh for hernia prevention. Randomized controlled trials (RCTs) published hereafter demonstrate conflicting outcomes.Methods and Analysis: A rapid guideline will be developed and reported in accordance with GRADE, GIN and AGREE-S standards. …”
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Clinical use of HIV integrase inhibitors: a systematic review and meta-analysis. by Peter Messiaen, Annemarie M J Wensing, Axel Fun, Monique Nijhuis, Nele Brusselaers, Linos Vandekerckhove

“…Multiple reviewers independently screened 1323 citations in duplicate to identify randomized controlled trials, nonrandomized controlled trials and cohort studies on integrase inhibitor use in clinical practice. …”
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Mesenchymal Stem Cell Therapy for Acute Myocardial Infarction: Protocol for a Systematic Review and Meta-Analysis by Michael Vincent DiCaro, Brianna Yee, KaChon Lei, Kavita Batra, Buddhadeb Dawn

“…However, these strategies are inadequate for replacing cells lost during tissue ischemia, thereby leaving behind noncontractile scar tissue. …”
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