Identification of Stable Reference miRNAs for miRNA Expression Analysis in Adult Neurogenesis Across Mouse and Human Tissues by Daniella Liana Levitis, Julia Si, Kushal Ravishankar, Michal Toborek, Minseon Park

“…Using small RNA sequencing, we identified the most stable miRNAs in primary mouse NPCs and hippocampal tissues and accessed their stability using NormFinder analysis. …”
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Spectral Analysis of Bioelectrical Activity in Experimentally Provoked Referred Pain – Motor Alterations Related to the Network of Trigger Points by Konieczny M, Matuska J, Domaszewski P, Pakosz P, Herrero P, Santafe MM, Wotzka D, Zmarzły D, Skorupska E

“…Power spectral density analysis was then applied to assess the obtained signals.Results: Significant statistical differences in power spectral density were observed between the EXP and CON groups in thigh muscles, while no statistical differences were found in the pelvic girdle muscles. The most notable atypical bioelectrical activity was observed in the vastus lateralis, semitendinosus, and rectus femoris muscles.Results Importance: Visualization of motor alterations related to the trigger point network in the thigh due to noxious stimulation of the gluteus minimus trigger points.Conclusion: Noxiously provoked referred pain from the gluteus minimus muscle, confirmed by abnormal autonomic activity, is characterized by motor alternations of the thigh muscles, suggesting a network of latent trigger points. …”
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Extreme Elevations in Blood Pressure and All-Cause Mortality in a Referred CKD Population: Results from the CRISIS Study by James Ritchie, Francesco Rainone, Darren Green, Helen Alderson, Diana Chiu, Rachel Middleton, Donal O’Donoghue, Philip A. Kalra

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Locating Appropriate Reference Genes in Heteroblastic Plant <i>Ottelia cordata</i> for Quantitative Real-Time PCR Normalization by Panyang Guo, Runan Li, Jiaquan Huang, Liyan Yin

“…After analyzing the stability using five statistical methods (geNorm, normFinder, comparative ΔCt, bestKeeper, and comprehensive analysis), <i>PP2A</i> and <i>UBQ</i> were identified as the most stable genes. <i>BRCC36</i> was identified as a new reference gene in plants. …”
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Blood Bacterial Microbiota of the American Bison (<i>Bison bison</i>) in Northern Mexico: A Reference for Health and Conservation by Juan Carlos Ontiveros-Chacón, Cristina García-De La Peña, Joel Domínguez-Viveros, Guadalupe Nelson Aguilar-Palma, Verónica Ávila-Rodríguez, Josué Raymundo Estrada-Arellano, Quetzaly Karmy Siller-Rodríguez, Luis Manuel Valenzuela-Núñez, Jesús Vásquez-Arroyo, Juan Carlos Herrera-Salazar, Annely Zamudio-López, Judith Correa-Gómez

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Research note: Reference genes selection for gene expression analyses in reproductive turkey (Meleagris gallopavo) with yellow semen syndrome by Ewa M. Drzewiecka, Ewa Liszewska, Krzysztof Kozłowski, Andrzej Ciereszko, Mariola Słowińska

“…The current study aimed to evaluate the most accurate qPCR internal controls that might be used as first-choice-reference genes in studies on the reproductive tract, liver, and immune tissues and cells of adult male turkey breeders. …”
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Stability analysis of the reference standard of <i>Mycoplasma arginini</i> strain G230 used for testing biologicals for mycoplasmas by S. M. Sukhanova, Z. E. Berdnikova, O. V. Fadeikina, E. M. Petruchuk, A. S. Tikhonova

“…Statistical data processing was carried out by the serial dilution method using McCrady’s table with the calculation of the arithmetic mean of the most probable number (MPN) for the limiting dilution (titre). …”
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A stable combination of non-stable genes outperforms standard reference genes for RT-qPCR data normalization by Anis Djari, Guillaume Madignier, Christian Chervin, Benoît van der Rest, James J. Giovannoni, Mondher Bouzayen, Julien Pirrello, Elie Maza

“…Despite the emergence of microarrays and high-throughput sequencing, qPCR remains a standard method for gene expression analyses, with its data normalization step being crucial for ensuring accuracy. Currently, the most widely used normalization method is based on the use of reference genes, assumed to be stably expressed across all experimental conditions. …”
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Comparison of single bacteria and a bacterial reference community in a test against coated surfaces of varying copper content by Yen Ly-Sauerbrey, Ronja Anton, Laura Kopruch, Carolin Luisa Krämer, Carolin Luisa Krämer, Alessa L. Boschert, Alessa L. Boschert, Claudio Neidhöfer, Oliver Schwengers, Daniela Zander, Daniela Zander, Stefan Leuko

“…Additionally, the application of pure copper surfaces is high in cost and maintenance.MethodsHence, in this study we not only tested the antibacterial effectivity of different copper concentrations against single species, but also against a reference bacterial community representing the most abundant bacterial genera in public transport. …”
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Particularities in the “Oldie but Goldie” Tc-99m DMSA Renography: A Retrospective Reference Centre Overview of 931 Children by Irena Cristina Grierosu, Iuliana Magdalena Starcea, Wael Jalloul, Maria Adriana Mocanu, Roxana Alexandra Bogos, Tudor Ilie Lazaruc, Madalina Andreea Beldie, Ruxandra Tibu, Teodor Marian Ionescu, Cati Raluca Stolniceanu, Brindusa Casiana Acsinte, Cipriana Stefanescu, Alexandra Saviuc, Vlad Ghizdovat

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A point prevalence survey (PPS) of antimicrobial use amongst patients referred to tertiary care centre in Northern India by Dr Naveen Murali, Dr Nikhil Aravind, Prof Nusrat Shafiq, Dr Niveditha Krishnappa, Dr Ritin Mohindra, Dr Rishin Raj P, Dr Ashish Thomas George, Dr Asheema Khosla, Ms Himanshi Khera, Ms Rupamdeep Kaur, Mr Roop Kishor Soni

“…We collected data on all patients in the emergency unit triage areas between 8 am and 5 pm. All patients referred from any hospital, clinic and nursing home in the triage area till 11:59 pm of the previous day of the survey were assessed for antibiotic use, either ongoing or pre-administered during the stay in the referring unit. …”
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Oral reference dose (RfD) derivation for five bisphenol A alternatives integrating BMD and NOAEL/LOAEL approaches by Yan Xia, Wenhua Ma, Jing Cao, Yanpeng Gao, Yingxin Yu, Chaoyang Long

“…Bisphenols (BPs) are widely used in plastic manufacturing, food packaging, and other industrial applications, with bisphenol A (BPA) being one of the most extensively produced chemicals globally. However, due to its endocrine-disrupting properties, BPA has been linked to reproductive abnormalities, metabolic disorders, neurodevelopmental impairments, and other adverse health effects, leading to regulatory restrictions. …”
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The Meaning and Extension of Evident and Hidden Name of God in Ibrahim Ibn Omar's narrative by کاوس روحی برندق, نوروز امینی

“…One of the complex narratives in Shia references is the narrative of Ibrahim ibn Omar from Imam Sadiq about Hidden Name (al-Ism al-Maknun) of God and its relation to Most Beautiful Names (al-Asma al-Husna). …”
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Między światami. Rzeczywistość w literaturze – literatura w rzeczywistości – rzeczywistość literatury by Danuta Ulicka

“…This point of view offers the opportunity to (re)construct the process of continuity /discontinuity in the whole field of research focused on the problem of reference, which has been recognized as the most important one in Polish studies (as well as in Polish literature, and art) since its beginning in the first decade of the 20th century. …”
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Prediction of spirometry parameters using chest circumference in Sri Lankan boys aged 8-16 years by Guwani Liyanage, Bernard Deepal Wanniarachchi Jayamanne

“…Introduction: Most normative standards for spirometry are established based on height, weight and body mass index. …”
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Establishing the plant component of a tallgrass prairie restoration using a remnant reference ecosystem model: A case study by William Sluis, Katherine Kucera, Gary Sullivan

“…The most common clonal species in remnants became most abundant and spread rapidly beyond plot boundaries, while individual plot richness (64–72 species, mean 68.8) was similar to that found in remnant reference ecosystems (82 species). …”
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Identification and validation of reference genes for quantitative gene expression analysis under 409 and 415 nm antimicrobial blue light treatment by Beata Kruszewska-Naczk, Mariusz Grinholc, Aleksandra Rapacka-Zdonczyk

“…Three programs, RefFinder, geNorm, and NormFinder, indicated that this gene had the most stable expression in comparison to the other reference gene candidates. …”
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Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in Megalurothrips usitatus (thysanoptera: thripidae) by Qingfang Hou, Qingfang Hou, Linlin Yuan, Linlin Yuan, Haifeng Jin, Haifeng Jin, Han Yan, Han Yan, Fen Li, Fen Li, Shaoying Wu, Shaoying Wu

“…Comprehensive stability ranking of candidate reference genes was recommended by RefFinder.Results and DiscussionResults showed that ribosomal protein S (RPS) was the most suitable expression in insecticide treatment. …”
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Gender Differentials of Non-communicable Diseases: A Case Study of Sri Lanka with Special Reference to Cardiovascular Diseases, Diabetes and Cancer by Hansa Andradige, Liwan Liyanage

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A comprehensive approach to identify reliable reference gene candidates to investigate the link between alcoholism and endocrinology in Sprague-Dawley rats. by Faten A Taki, Abdel A Abdel-Rahman, Baohong Zhang

“…Our results showed that U87, 5S rRNA, GAPDH, and U5a were the most reliable gene candidates for reference genes in heart and brain tissue. …”
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