A multiplex real-time PCR assay for simultaneous detection of classical swine fever virus, African swine fever virus, and atypical porcine pestivirus by Xiang-peng SONG, Ying-ju XIA, Lu XU, Jun-jie ZHAO, Zhen WANG, Qi-zu ZHAO, Ye-bing LIU, Qian-yi ZHANG, Qin WANG

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Causal relationships between immune-related adverse events, immune cell characteristics, and plasma metabolites: insights from Mendelian randomization study by Zhiyuan Shi, Zhao Sun, Dingding Zhang, Lin Zhao

“…This discovery serves as a valuable reference for the judicious application of immunotherapy in future endeavors.…”
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Targeting a pathogenic cryptic exon that drives HLRCC to induce exon skipping by Siddhardha S. Maligireddy, Mariana D. Mandler, Judith C. Lunger, Madeline Yuen, Sneha Kulkarni, Alexendar R. Perez, Christina M. Fitzsimmons, Daniel R. Crooks, Raj Chari, W. Marston Linehan, Pedro J. Batista

“…Furthermore, we were able to show that ASOs can be used to shift the balance between the FH mRNA isoforms originated from the reference and the variant allele in patient-derived fibroblasts using ASOs. …”
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Meropenem/vaborbactam activity against carbapenem-resistant Klebsiella pneumoniae from catheter-related bloodstream infections by Francesca Sivori, Massimo Francalancia, Mauro Truglio, Ilaria Cavallo, Carmelina Pronesti, Giorgia Fabrizio, Ilaria Celesti, Andrea Cazzani, Lorenzo Furzi, Fulvia Pimpinelli, Enea Gino Di Domenico

“…Phenotypic assays revealed enhanced biofilm formation and metabolic activity compared to the reference strain Kp ATCC 13883 in the absence of hypervirulence-associated genes. …”
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Transcriptome Profiling Reveals Genetic Basis of Muscle Development and Meat Quality Traits in Chinese Congjiang Xiang and Landrace Pigs by Jiada Yang, Qiaowen Tang, Chunying Sun, Qiuyue Li, Xiaoyu Li, Lu Hou, Yi Yang, Kang Yang

“…RNA sequencing was performed on muscle tissues from ten individuals of each breed, generating 874.5 million raw reads with an average mapping rate of 89.3% to the pig reference genome. (3) Results: Transcriptional profiling revealed distinct expression patterns with 785 genes exclusively expressed in CX pigs and 457 genes unique to LAN pigs, while 7099 co-expressed genes were shared by both breeds. …”
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Skin transcriptomic and selection signature analyses identify ASIP as a key gene in cattle coat color determination by Xin Wang, Longxin Xu, Di Zhou, Yanli Lv, Junda Wu, Yuanfeng Zhao, Mengmeng Ni, Wenzhang Zhou, Kaikai Zhang, Hua Wang, Jipan Zhang

“…Notably, the mutation type was predominant in Simmental cattle, while the reference allele was more common in Angus cattle.ConclusionBased on the skin transcriptomic and genomic analyses, we found that ASIP was significantly differential expressed between black and brown cattle breeds and under strong positive selection. …”
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Identification of genetic determinants of antibiotic resistance in Helicobacter pylori isolates in Vietnam by high-throughput sequencing by Phuc Hoang Bui, Trang Ngoc Minh Cao, Thanh Truc Tran, Takashi Matsumoto, Junko Akada, Yoshio Yamaoka

“…The primary resistance of H. pylori to commonly used antibiotics was studied, and high-throughput next-generation sequencing (NGS) was employed to discover genetic determinants of resistance using a reference-based approach. A total of 123 H. pylori strains were cultured and tested for antibiotic susceptibility using an E test. …”
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Identification of the Fanconi Anemia Complementation Group I Gene, FANCI by Josephine C. Dorsman, Marieke Levitus, Davy Rockx, Martin A. Rooimans, Anneke B. Oostra, Anneke Haitjema, Sietske T. Bakker, Jûrgen Steltenpool, Dezsö Schuler, Sheila Mohan, Detlev Schindler, Fré Arwert, Gerard Pals, Christopher G. Mathew, Quinten Waisfisz, Johan P. de Winter, Hans Joenje

“…To identify the gene underlying Fanconi anemia (FA) complementation group I we studied informative FA-I families by a genome-wide linkage analysis, which resulted in 4 candidate regions together encompassing 351 genes. …”
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Whole RNA-Seq Analysis Reveals Longitudinal Proteostasis Network Responses to Photoreceptor Outer Segment Trafficking and Degradation in RPE Cells by Rebecca D. Miller, Isaac Mondon, Charles Ellis, Anna-Marie Muir, Stephanie Turner, Eloise Keeling, Htoo A. Wai, David S. Chatelet, David A. Johnson, David A. Tumbarello, Andrew J. Lotery, Diana Baralle, J. Arjuna Ratnayaka

“…RNA-seq analysis of the highly differentiated human retinal pigment epithelial (RPE) cell-line ARPE-19, cultured on transwells for ≥4 months, yielded 44,909 genes showing 83.35% alignment with the human reference genome. These included mRNA transcripts of RPE-specific genes and those involved in retinopathies. …”
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Integrated metagenomics and transcriptomics analysis reveals pathways associated with oral periapical lesions formation and progression by Alaa Muayad Altaie, Thenmozhi Venkatachalam, Kiran Patil, Farah Al-Marzooq, Surendra Singh Rawat, Ali Al Qabbani, Mohammed Amjed Alsaegh, Lakshman P. Samaranayake, Sameh S.M. Soliman, Rifat Hamoudi

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Identification of genetic locus with resistance to take-all in the wheat-Psathyrostachys huashanica Keng introgression line H148 by Sheng-sheng BAI, Han-bing ZHANG, Jing HAN, Jian-hui WU, Jia-chuang LI, Xing-xia GENG, Bo-ya LÜ, Song-feng XIE, De-jun HAN, Ji-xin ZHAO, Qun-hui YANG, Jun WU, Xin-hong CHEN

“…Sixty-two genes were found in the target interval of the Chinese Spring reference genome sequence. According to the functional annotation of genes, two protein genes that can improve the systematic resistance of plant roots were predicted as candidate genes. …”
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Heredity and gene mapping of a novel white stripe leaf mutant in wheat by Hui-juan LI, Zhi-xin JIAO, Yong-jing NI, Yu-mei JIANG, Jun-chang LI, Chao PAN, Jing ZHANG, Yu-long SUN, Jun-hang AN, Hong-jie LIU, Qiao-yun LI, Ji-shan NIU

“…In comparison to the reference genome sequence of Chinese Spring (CS), wsl is located in a 91-Mb region from 88 Mb (Xgwm518) to 179 Mb (Xgpw7651) on chromosome 6BS. …”
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Gene expression dynamics in Bacillus cereus and Bacillus subtilis treated with Thymus vulgaris and Origanum vulgare subsp. hirtum essential oils by Fabrizio Anniballi, Chiara Purgatorio, Annalisa Serio, Concetta Scalfaro, Silvia Taglieri, Antonello Paparella

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Spatio-temporal diversity and genetic architecture of pyrantel resistance in Cylicocyclus nassatus, the most abundant horse parasite by Sallé, Guillaume, Courtot, Élise, Cabau, Cédric, Parrinello, Hugues, Serreau, Delphine, Reigner, Fabrice, Gesbert, Amandine, Jacquinot, Lauriane, Lenhof, Océane, Aimé, Annabelle, Picandet, Valérie, Kuzmina, Tetiana, Holovachov, Oleksandr, Bellaw, Jennifer, Nielsen, Martin K., von Samson-Himmelstjerna, Georg, Valière, Sophie, Gislard, Marie, Lluch, Jérôme, Kuchly, Claire, Klopp, Christophe

“…Here we have leveraged ultra-low input sequencing protocols to build a reference genome for the most prevalent horse strongyle species. …”
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Asynchronous replication in oncological patients by G. F. Mikhailova, V. V. Tsepenko, T. G. Shkavrova, E. V. Goloub

“…One of the most important marks of epigenetic misbalance of cell genome is an replication asynchrony of genes with biallelic expression. …”
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Comparing the Transcriptomes of Two Different Tissues in Helicoverpa assulta (Guenée) by Hailing Zhang, Kuiyin Li, Yubo Zhang

“…Our findings based on transcriptome sequencing, data assembly, and functional gene annotation of two different tissues in H. assulta will provide a valuable reference for further excavation and study of functional genes in H. assulta.…”
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miR-383-3p and miR-6951-3p activate cell proliferation through the regulation of genes related to hypertelorism by Chihiro Iwaya, Junichi Iwata, Junichi Iwata

“…By searching the Mouse Genome Informatics (MGI) database and reviewing full-text references, we identified a total of 31 genes potentially related to hypertelorism. …”
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