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Effect of multiple plasma freeze-thaw cycles on non-invasive prenatal testing
Published 2025-01-01“…The quantitative yield was assessed by fluorometry, while the fragmentation and NIPT data — using realtime polymerase chain reaction (PCR) and high-throughput sequencing, respectively.Results. …”
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ASSOCIATION OF THE VARIANTS OF NUCLEOTIDE SEQUENCE OF THE GENE ADIPOQ WITH PLASMATIC LEVEL OF ADIPONECTIN, THE GRADE OF CORONARY ATHEROSCLEROSIS SEVERITY AND RATE OF CARDIOVASCULAR...
Published 2018-09-01“…Genetic variants rs17300539, rs182052 and rs266729 gene ADIPOQ were measured realtime (Applied Biosystems, 7500 Real Time PCR System). …”
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Human β-defensin-3gene expression in mucosa of ORL organs
Published 2022-07-01“…Estimation of hBD-3 and β-actin gene expression was performed by reverse transcription and realtime PCR. In the nasal and sino-nasal mucosa, only negligible expression levels were detected in 14.29-33.33% of samples, most often in the specimens from the middle nasal passage and ethmoid labyrinth polyps (53.84%), being absent in hypertrophic inferior turbinate. …”
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Genetic and biochemical characterization of staphylococci occurring in Novosibirsk, Russia
Published 2018-01-01“…Identification of coagulase positive strains was performed using a standard biochemical method and by realtime PCR of the coa gene. 100 % coincidence between the presence of the gene and coagulase activity for S. aureus strains was recorded, which suggests that detection of the coa gene can be used as a correct method for S. aure us identification. …”
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Association of T715P (RS6136), M62I (RS2228315), S290N (RS6131), V640L (RS6133) polymorphisms in the P-selectin gene and its ligand with acetylsalicylic acid resistance in patients...
Published 2019-11-01“…The aggregation study was carried out with an optical aggregometer using ADP inducers (5 pM) and arachidonic acid (1 µМ) before CABG, on 1-3 and 8-10 days after surgical treatment.DNA samples were examined for the presence of T715P (rs6136), M62I (rs2228315), S290N (rs6131), V640L (rs6133) polymorphisms in the P-selectin gene using realtime PCR with allele-specific primers.Results. …”
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