Real-time fluorescence growth curves for viable bacteria quantification in foods by Yajing Chen, Yanlin Chen, Siying Tang, Biao Tang, Shengbin He

Subjects: “…Bacterial quantification…”
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Potential influence of the first PCR cycles in real-time comparative gene quantifications by Hege Karin Nogva, Knut Rudi

“…Differences in the amplification efficiencies between type 1, 2, and 3 products may explain part of the error in the gene copy number determinations using real-time PCR comparative gene quantifications. Future applications of real-time PCR quantifications should account for the effect of the first few PCR cycles on the conclusions drawn.…”
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Real-Time Fluorescence-Based Method for Dynamic Quantification of Droplet Network Assembly by Alessia Faggian, Federica Casiraghi, Martin M. Hanczyc

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Multiplex real-time single nucleotide polymorphism detection and quantification by quencher extension by Knut Rudi, Monika Zimonja, Sigrun E. Hannevik, Signe M. Drømtorp

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Edge Artificial Intelligence (AI) for real-time automatic quantification of filariasis in mobile microscopy. by Lin Lin, Elena Dacal, Nuria Díez, Claudia Carmona, Alexandra Martin Ramirez, Lourdes Barón Argos, David Bermejo-Peláez, Carla Caballero, Daniel Cuadrado, Oscar Darias-Plasencia, Jaime García-Villena, Alexander Bakardjiev, Maria Postigo, Ethan Recalde-Jaramillo, Maria Flores-Chavez, Andrés Santos, María Jesús Ledesma-Carbayo, José M Rubio, Miguel Luengo-Oroz

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Comparison of methods for relative quantification of gene ex- pression using real-time PCR by Luka BOLHA, Daliborka DUŠANIĆ, Mojca NARAT, Irena OVEN

“…Quantitative real-time PCR (qPCR) has become a widely used tool for quantifying gene expression. …”
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Digital PCR outperforms quantitative real-time PCR for the detection and quantification of major periodontal pathobionts by Haris Munjaković, Katja Povšič, Mario Poljak, Katja Seme, Rok Gašperšič, Lucijan Skubic

“…Background This study comparatively evaluated the analytical and diagnostic performance of a multiplex digital polymerase-chain reaction (dPCR) assay and a quantitative real-time PCR (qPCR) for the simultaneous detection and quantification of periodontal pathobionts: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Fusobacterium nucleatum.Materials and Methods Subgingival plaque samples from 20 periodontitis patients and 20 periodontally healthy controls were analyzed. …”
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Real-time quantification of activated sludge concentration and viscosity through deep learning of microscopic images by Hewen Li, Yu Tao, Tiefu Xu, Hongcheng Wang, Min Yang, Ying Chen, Aijie Wang

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Rapid quantification of microRNAs in plasma using a fast real-time PCR system by William John Andrews, Eoin Daniel Brown, Margaret Dellett, Ruth Esther Hogg, David Arthur Simpson

“…One rate-limiting factor in miRNA detection is the time taken to perform quantitative real-time PCR (qPCR) amplification. We therefore evaluated the ability of a novel thermal cycler to perform this step in less than 10 minutes. …”
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Real-time imaging and quantification of amyloid-beta peptide aggregates by novel quantum-dot nanoprobes. by Kiyotaka Tokuraku, Meg Marquardt, Tsuneya Ikezu

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Quantification of Enterovirus RNA in Sludge Samples Using Single Tube Real-Time RT-PCR by S. Monpoeho, A. Dehée, B. Mignotte, L. Schwartzbrod, V. Marechal, J.-C. Nicolas, S. Billaudel, V. Férré

“…PCR inhibitors were removed by extracting viral RNA (after virus concentration) using the RNeasy® mini kit with added polyvinylpyrrolidone (PVP) and running the amplification reaction with a mixture containing PVP and T4 gene 32 protein. This real-time quantification of enterovirus RNA allows large numbers of samples to be screened. …”
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Quantification of Gardnerella vaginalis, Atopobium vaginae and Lactobacillus spp. in bacterial vaginosis by Nedzib Numanovic, Snezana Ribis, Jelena Cukic, Dane Nenadic, Aleksandar Zivanovic, Predrag Sazdanovic, Violeta Ninkovic, Dejan Baskic

“…Nevertheless, real-time PCR has been more sensitive in diagnosis of bacterial vaginosis. …”
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Photoplethysmography-based HRV analysis and machine learning for real-time stress quantification in mental health applications by Ying-Ying Tsai, Yu-Jie Chen, Yen-Feng Lin, Fan-Chi Hsiao, Ching-Han Hsu, Lun-De Liao

“…Baseline and stress-state PPG data are collected, converted into HRV values, and input into the model for stress quantification. The Stroop test is used to elicit stress responses. …”
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Comparison of a novel real-time PCR method (RTA) and Artus RG for the quantification of HBV DNA and HCV RNA by Sibel Aydogan, Ziya Cibal Acikgoz, Aysegul Gozalan, Fisun Kirca, Tuba Muderris, Elife Ozturk

“… Introduction: The purpose of this study was to evaluate and compare two manual isolation and real-time (RT) polymerase chain reaction (PCR) kits (RTA RT-PCR with RTA isolation kit and Artus RG RT-PCR with QIAamp isolation kit) for molecular diagnosis of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections. …”
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Real-Time Detection and Quantification of Rail Surface Cracks Using Surface Acoustic Waves and Piezoelectric Patch Transducers by Mohsen Rezaei, Sven Eck, Sebastian Fichtenbauer, Jürgen Maierhofer, Reinhard Klambauer, Alexander Bergmann, David Künstner, Dino Velic, Hans-Peter Gänser

“…This paper presents a novel wayside rail monitoring system for real-time detection and quantification of rail surface cracks with sub-millimeter precision. …”
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Ultrasensitive detection and quantification of bovine Deltapapillomavirus in the semen of healthy horses by Anna Cutarelli, Francesca De Falco, Francesco Serpe, Simona Izzo, Giovanna Fusco, Cornel Catoi, Sante Roperto

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New screening and quantification method by UHPLC-MS/MS of 36 new synthetic cathinones in hair. Application to real cases by Alfredo Fabrizio Lo Faro, Anastasio Tini, Giorgi Kobidze, Giulio Mannocchi, Massimo Gottardi, Francesco Paolo Busardò, Giuseppe Basile, Bezhan Chakvetadze

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Holistic uncertainty quantification and attribution for real-time seasonal streamflow predictions: Insights from input, parameter and initial condition by Li Liu, Peng Zhou, Yue-Ping Xu, Chaohao Zheng, Lu Wang, Xiao Liang, Yuxue Guo

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Quantification of Viable <i>Salmonella</i> by Propidium Monoazide Real-Time PCR After Long-Term Storage of Peanut Products by Aline M. von Hertwig, André A. Pereira, Dionisio Pedro Amorim Neto, Maristela S. Nascimento

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A Novel and Advanced Diagnostic Approach toward Paracidovorax citrulli Causing Bacterial Fruit Blotch in Watermelon by Direct SYBR Green Real-Time PCR Assay by Hyeonheui Ham, Bang Wool Lee, Kyongnim Kim, Woohyung Lee, Yong Hwan Lee, Dong Suk Park

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