The clinical potential of PDL-1 pathway and some related micro-RNAs as promising diagnostic markers for breast cancer by Eman A. Al-Sharabass, Motawa E. EL-Houseini, Heba Effat, Sherif Abdelaziz Ibrahim, Mona S. Abdellateif

“…Methods Expression levels of programmed cell death protein 1 (PD-1), cytotoxic T-lymphocyte–associated antigen 4 (CTLA-4), programmed death-ligand 1 (PD-L1), Forkhead box P3 (FOXP3), miR-155, and miR-195 were assessed in the peripheral blood of 90 BC patients compared to 30 healthy controls using quantitative real-time PCR (qRt-PCR). …”
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Mortality outcomes with hydroxychloroquine and chloroquine in COVID-19 from an international collaborative meta-analysis of randomized trials by Cathrine Axfors, Andreas M. Schmitt, Perrine Janiaud, Janneke van’t Hooft, Sherief Abd-Elsalam, Ehab F. Abdo, Benjamin S. Abella, Javed Akram, Ravi K. Amaravadi, Derek C. Angus, Yaseen M. Arabi, Shehnoor Azhar, Lindsey R. Baden, Arthur W. Baker, Leila Belkhir, Thomas Benfield, Marvin A. H. Berrevoets, Cheng-Pin Chen, Tsung-Chia Chen, Shu-Hsing Cheng, Chien-Yu Cheng, Wei-Sheng Chung, Yehuda Z. Cohen, Lisa N. Cowan, Olav Dalgard, Fernando F. de Almeida e Val, Marcus V. G. de Lacerda, Gisely C. de Melo, Lennie Derde, Vincent Dubee, Anissa Elfakir, Anthony C. Gordon, Carmen M. Hernandez-Cardenas, Thomas Hills, Andy I. M. Hoepelman, Yi-Wen Huang, Bruno Igau, Ronghua Jin, Felipe Jurado-Camacho, Khalid S. Khan, Peter G. Kremsner, Benno Kreuels, Cheng-Yu Kuo, Thuy Le, Yi-Chun Lin, Wu-Pu Lin, Tse-Hung Lin, Magnus Nakrem Lyngbakken, Colin McArthur, Bryan J. McVerry, Patricia Meza-Meneses, Wuelton M. Monteiro, Susan C. Morpeth, Ahmad Mourad, Mark J. Mulligan, Srinivas Murthy, Susanna Naggie, Shanti Narayanasamy, Alistair Nichol, Lewis A. Novack, Sean M. O’Brien, Nwora Lance Okeke, Léna Perez, Rogelio Perez-Padilla, Laurent Perrin, Arantxa Remigio-Luna, Norma E. Rivera-Martinez, Frank W. Rockhold, Sebastian Rodriguez-Llamazares, Robert Rolfe, Rossana Rosa, Helge Røsjø, Vanderson S. Sampaio, Todd B. Seto, Muhammad Shahzad, Shaimaa Soliman, Jason E. Stout, Ireri Thirion-Romero, Andrea B. Troxel, Ting-Yu Tseng, Nicholas A. Turner, Robert J. Ulrich, Stephen R. Walsh, Steve A. Webb, Jesper M. Weehuizen, Maria Velinova, Hon-Lai Wong, Rebekah Wrenn, Fernando G. Zampieri, Wu Zhong, David Moher, Steven N. Goodman, John P. A. Ioannidis, Lars G. Hemkens

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SARM1 deletion inhibits astrogliosis and BBB damage through Jagged-1/Notch-1/NF-κB signaling to improve neurological function after ischemic stroke by Yan Qiong Fu, Yu Zheng, Zhuo Li Li, Xin Yi Huang, Xiao Wan Wang, Mai Yin Cui, Yun Qi Zhang, Bing Rui Gao, Chan Zhang, Xiao Xiao Fan, Yong Jian, Bai Hui Chen

“…Immunohistochemistry, western blot, and real-time PCR analyses revealed that compared with the wild-type (WT) mice, SARM1−/− mice exhibited reduced astrocytic proliferation, increased anti-inflammatory astrocytes, decreased glial scar formation in the infarct zone on day 7 after ischemic injury. …”
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PD-1-dependent therapeutic effect of Trichinella spiralis cystatin on myocardial infarction in a mice model by Weixiao Zhang, Wenhui Yin, Hongtao Wang, Lingqin Wu, Chang Li, Xinyu Peng, Xiang Li, Kaibo Jiang, Huiqi Yang, Chenyue Dang, Erhe Gao, Qiwang Jin, Xiaodi Yang

“…Trichinella spiralis cystatin (Ts-Cys) has been shown to regulate macrophage polarization and alleviate various inflammatory and immune-related diseases. Programmed cell death-1 (PD-1) is a crucial checkpoint receptor molecule and highly involved in maintaining immune tolerance. …”
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Prevalence of oral HPV infection and genotype distribution in Iranian children during the early ages of life by Mona Sadat Larijani, Amir Javadi, Alireza Fahimzad, Rahim Soleimani, Farbod Tabatabaei, Amir Houshang Nejadeh, Mahboubeh Jamshidi, Anahita Bavand, Ladan Moradi, Fatemeh Ashrafian, Amitis Ramezani

“…DNA extraction was done and Real-Time PCR was performed to characterize HPV infection. …”
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Net ownership, utilization and malaria burden in the context of Piperonyl butoxide-LLINs intervention in western Kenya. by Job Oyweri, Patrick O Onyango, Maxwell G Machani, Josephat Bungei, Sammy Cheruiyot, Yaw A Afrane, Ming-Chieh Lee, Daibin Zhong, Guofa Zhou, John Githure, Harrysone Atieli, Guiyun Yan

“…Finger prick blood smears were collected on slides for microscopic examination, while dry blood spots (DBS) on filter paper were collected for real-time PCR (RT-PCR) diagnosis of Plasmodium infection in both intervention groups. …”
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Comparison of AI and resident doctors in diagnosing and managing acute respiratory cases: a retrospective study by Abdurrahmaan Manga, Saher Sultan, Ammarah Yasmeen, Fasihul Khan

“…Further research is needed to explore its integration into clinical workflows and training programs, as well as its ability to augment decision-making to enhance, rather than replace, resident doctor expertise.3…”
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Optimized production of 89Zr as a medical radioisotope on a variable energy cyclotron and external beam-line by Diana Cocioabă, Simona Baruta, Liviu Crăciun, Radu Leonte, Andrei Necsoiu, Maria-Roxana Tudoroiu-Cornoiu, Alexandru Jipa, Dana Niculae

“…Monte Carlo programs simulated the irradiation geometry and estimated the activity and irradiation yields produced by the 89Y(p, n)89Zr reaction, at the process optimal parameters. …”
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Variables asociadas a alteraciones cognitivas en una cohorte de personas sobrevivientes por COVID-19 en hospital de tercer nivel de México by M. Rodríguez-Rodríguez, Y. Rodríguez-Agudelo, F.J. Soto-Moreno, A. García-Santos, D. López-González, M. González-Navarro, F. Paz-Rodríguez, M. Chávez-Oliveros, S. Lozano-Tovar, K. González-Alonso, A. Castorena-Maldonado, R. Carrillo-Mezo, O. Marrufo-Meléndez, A. Gutiérrez-Romero, M. del Río Quiñones, A. Arauz-Góngora, S. Avila-Rios

“…Our findings highlight the clinical need for longitudinal programs capable of evaluating the real impact of SARS-CoV-2 infection on the central nervous system, particularly in the cognitive and emotional domains.…”
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Core effectorome of Fusarium oxysporum f. sp. conglutinans deciphered using a multiomics method exhibits diverse roles following infection in cabbage by Miaomiao Xing, Cunbao Zhao, Xizhe Sun, Ailing Zhou, Yong Wang, Jialei Ji, Aisong Zeng, Biju V. Chellappan, Yangyong Zhang, Lisong Ma, Honghao Lv

“…Here, we presented the chromosome-level genome assembly of the Focn race 1 strain FGL03-6 via a single-molecule real-time sequencing platform, resequenced the genomes of 23 strains belonging to races 1 and 2, and performed genome-wide identification of Focn effectors using multiomics data generated from the Focn-cabbage interaction. …”
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Ceftolozane/tazobactam use and emergence of resistance: a 4-year analysis of antimicrobial susceptibility in Pseudomonas aeruginosa isolates in a tertiary hospital by Alessandra Imeneo, Laura Campogiani, Laura Campogiani, Pietro Vitale, Andrea Di Lorenzo, Andrea Di Lorenzo, Grazia Alessio, Davide Natale Abate, Maria Grazia Celeste, Anna Altieri, Cartesio D’Agostini, Cartesio D’Agostini, Vincenzo Malagnino, Vincenzo Malagnino, Massimo Andreoni, Massimo Andreoni, Marco Iannetta, Marco Iannetta, Loredana Sarmati, Loredana Sarmati

“…BackgroundCeftolozane/tazobactam (C/T) was temporarily withdrawn from December 2020 to February 2022: this forced unavailability created the conditions to study how drug discontinuation might influence Pseudomonas aeruginosa (PA) resistance reversibility in a real-life setting.MethodsClinically relevant PA isolates collected between January 1st 2019 and February 22nd 2023 with a C/T susceptibility test available were included. …”
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Molecular and serological evidence of chikungunya virus among dengue suspected patients in Sri Lanka by Mya Myat Ngwe Tun, Maurine Mumo Mutua, Shingo Inoue, Yuki Takamatsu, Satoshi Kaneko, Takeshi Urano, Rohitha Muthugala, Lakkumar Fernando, Menaka Hapugoda, YINS Gunawardene, Kouichi Morita

“…We performed quantitative real-time RT-PCR (RT-qPCR) to detect the CHIKV genome and conducted serological tests for anti-CHIKV IgM and IgG antibodies on all samples. …”
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Nasopharyngeal Carriage, Serotype Distribution, and Antimicrobial Susceptibility of <i>Streptococcus pneumoniae</i> Among PCV13-Vaccinated and -Unvaccinated Children in Iran by Fatemeh Ashrafian, Mona Sadat Larijani, Saiedeh Haji Maghsoudi, Delaram Doroud, Alireza Fahimzad, Zahra Pournasiri, Elham Jafari, Masoumeh Parzadeh, Sara Abdollahi, Elham Haj Agha Gholizadeh Khiavi, Anahita Bavand, Morvarid Shafiei, Mahdi Rohani, Amitis Ramezani

“…<i>S. pneumoniae</i> was identified through a combination of culture methods and biochemical tests, confirmed by real-time PCR. Serotyping was achieved using <i>cpsB</i> sequencing, and the minimum inhibitory concentration method was employed to assess antibiotic resistance. …”
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Armonizando el cuidado: efectos de la música en directo en el bienestar de los pacientes oncológicos e intérpretes en el ámbito hospitalario by Raquel Caballero de la Calle, María Encarnación Fernández Contreras, Miguel Ángel García Garrido, Patricia Ferrero Sereno, Ana Ruiz Rodríguez, Raquel Badillo de Santos, María Nieves Moro-Tejedor, Miriam Leñero-Cirujano

“…This research project stems from a collaborative and interdisciplinary effort between two undergraduate programs: Nursing and Musical Performance. Objectives. …”
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Claudin-9 (CLDN9) promotes gastric cancer progression by enhancing the glycolysis pathway and facilitating PD-L1 lactylation to suppress CD8+ T cell anti-tumor immunity by Xingbin Hu, Wenhao Ouyang, Haizhu Chen, Zhihong Liu, Zijia Lai, Herui Yao

“…Subsequently, we evaluated the mRNA and protein levels of CLDN9 in GC using quantitative real-time polymerase chain reaction (PCR) (qRT-PCR), Western blotting (WB), and immunohistochemistry (IHC). …”
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Clinical factors influencing retreatment with anti-PD-(L)1 therapies after treatment in early-stage cancers: a modified Delphi consensus study by Christos Chouaïd, Lajos Pusztai, Federico Cappuzzo, Phillip Parente, Robert Jones, Vernon K Sondak, Masayoshi Nagata, Raquel Aguiar-Ibáñez, Yosuke Hirasawa, Ryuichi Mizuno, Smita Kothari, Carmel Spiteri, Alexander Sun, Chris Elder, Masaru Ishida, Seung Hyeun Lee, David Okonji, Bhavesh Shah, Dominihemberg Ferreira, Andrea Lauer, Amrit Kaliasethi, Carol Kao, Jan McKendrick

“…Anti-programmed death (ligand) 1 (anti-PD-(L)1) therapies were first introduced in the metastatic setting and have since been approved and reimbursed for treating early-stage cancers in the adjuvant, perioperative, and neoadjuvant settings in many cancer types. …”
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Investigating the Relationship between Changes in the Expression Level of the CLU Gene and the Prognosis of Gastric Cancer Patients by Arezoo Farhadi, Erfan Cheraghi, Nastaran Farahani, Fatemeh Khodabandehloo, Mohammad Foad Heidari, Reza Heydari, Mehdi Ghorbani, Mahmood Vahidi, Javad Behroozi

“…The relative gene expression of CLU was assessed using quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR). …”
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The Impact of Digital Technology–Based Exercise Combined With Dietary Intervention on Body Composition in College Students With Obesity: Prospective Study by Chengyuan Hu, Zixin Lv, Jieping Zhu, Chunyuan Lai, Dongjuan Guo, Maolin Chen, Xiaoyan Cheng, Mingxin Rao, Xinyou Zhou, Liqiang Su

“… BackgroundLifestyle interventions are a critical component of weight loss programs, yet digital, personalized, and theory- and evidence-based lifestyle interventions remain limited. …”
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Effect of oxalic acid on Ca²⁺ concentration and signaling pathways in plants by Li Yang, Zhao Yuan, Xu Youping, Wang Jipeng, Cai Xinzhong

“…The known roles of OA in pathogenicity include lowering extracellular pH value and thus increasing activity of CWDEs, altering redox status to regulate accumulation of reactive oxygen species (ROS) and programmed cell death (PCD), and having direct toxicity to plant cells. …”
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Correction possibilities of drug-induced liver toxicity in the treatment of patients with blood system tumors by V. B. Larionova, A. V. Snegovoy

“…The results obtained were the basis for the development of supportive therapy programs to prevent and reduce liver toxicity during chemotherapy. …”
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