Analysis of transcriptome and chromatin accessibility changes during the differentiation of human embryonic stem cells into neural progenitor cells by LI Linying, CAI Xiaodong, TONG Ran, YANG Chen, WANG Zhiming, HE Xiaoyu, MA Ziyue, ZHANG Feng, LI Lingjie, ZHOU Junmei

“…Motif enrichment analysis revealed that transcription factor genes such as DLX1 and LHX2 might play an important role in the differentiation process from hESCs into NPCs. Integrated analysis of RNA-seq and ATAC-seq data revealed that overlapping genes with high expression at the NPC stage were mainly enriched in axon guidance, forebrain development, and neuron migration. …”
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Priority setting for biomedical research including Covid-19 in the northeastern part of Iran by Rahim Akrami, Abbas Badakhshan, Masoumeh Hashemian, Masoud Hiteh, Bahareh Amin, Mohammad Shafi Mojadadi, Mansoureh Feizabadi, Maasoumeh Aran, Najmeh Mahmoodabadi, Maryam Goudarzian, Salehabadi Raha, Alireza Darvishi, Homeyra Vahdati, Taibeh Nowrouzinejad, Akbar Pejhan, Mohsen Koushan, Nasrin Fazel, Ahmad Allahabadi, Abolfazl Rahmani Sani, Alireza Ghorbani, Mohammad Hassan Rakhshani, Moosaalreza Tadayonfar, Forough Mortazavi, Akram Kooshki, Roya Akbarzadeh, Mojtaba hadavifar, Ali Delbari, Hafez Heydari Zarnagh, Fatemeh Ghaffarirad, Majid Fallahi, Reza Frozen, Behnaz Souizi, Jila Agah, Ramezan Ali Khamirchi, Mohammad Shourideh Yazdi, Hessam Ghassemof, Houman Kamranian, Mehdi Molavi, Hossein Assarzadeh, Hassan Salehipour, Nasrin Hashemian Nejad, Mohammadreza Sadr, Seyed Mehdi Mirhamidi, Abolfazl Shakiba, Seyed Mehdi Razavi, Mahdi Motakeffar, Fatemeh Nodeh, Mohammad Reza Shegarf Nakhaie, Omid Gholami, Freshteh Ghorat, Mohammad Mohammad-Zadeh, Alireza Moslem, Kazem Zendehdel

“…Materials & Methods: we used both qualitative and quantitative approaches to determine the public health problems and health research priorities.The processes for priority setting included preparation, problem finding, idea generation, data cleaning and statement development, ranking, and dissemination. …”
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Videomängude disain noortele täiskasvanutele. Psühholoogilise heaolu edendamine koosloome kaudu / Participatory Video Game Design for Young Adults: Enhancing Psychological Well-bei... by Taavi Varm

“…Quantitative data were supported by open-ended reflections in which participants articulated new insights into game mechanics, storytelling, teamwork, and their own emotional experiences. …”
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Readiness for scale up following effectiveness-implementation trial: results of scalability assessment of the Community Partnership Program for diabetes self-management for older a... by Melissa Northwood, Tracey Chambers, Kathryn Fisher, Rebecca Ganann, Maureen Markle-Reid, Marie-Lee Yous, Ron Beleno, Gary Gaudet, Andrea Gruneir, Helen Leung, Craig Lindsay, Kasia Luebke, Gail Macartney, Ethel Macatangay, Janet MacIntyre, Carolyn MacPhail, William Montelpare, Allan Morrison, Lisa Shaffer, Martha St. Pierre, Frank Tang, Catharine Whiteside

“…The scalability assessment process was a practical method to generate concrete strategies to facilitate the uptake of the CPP into practice. …”
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Needs assessment for a decision support tool in oral cancer requiring major resection and reconstruction: a mixed-methods study protocol by David Goldstein, John R de Almeida, Rosemary Martino, Douglas B Chepeha, David Forner, Paul Hong, Martin Corsten, Valeria E Rac, Andrew G Shuman, Anna M Sawka, Jonathan C Irish, Dale H Brown, S Mark Taylor, Patrick J Gullane, Jonathan R Trites, Ralph Gilbert, Matthew H Rigby, Jolie Ringash

“…Thematic analysis will be used to analyse transcripts of interviews. Quantitative and qualitative components of the study will be integrated through triangulation, with matrix developed to promote visualisation of the data.Ethics and Dissemination This study has been approved by the research ethics boards of the Nova Scotia Health Authority (Halifax, Nova Scotia) and the University Health Network (Toronto, Ontario). …”
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Enhancing Sustainable Manufacturing in Industry 4.0: A Zero-Defect Approach Leveraging Effective Dynamic Quality Factors by Rouhollah Khakpour, Ahmad Ebrahimi, Seyed Mohammad Seyed Hosseini

“…The current work aims to present the development of ZDM by focusing on dynamic factors affecting product quality in order to improve sustainability in manufacturing processes in the era of industry 4.0.</p> <p style="text-align: left;">Utilizing ZDM philosophy along with technological advances in cyber physical systems (CPS) such as IoT, big data, and advanced data analytics creates remarkable challenges and opportunities to develop new methodologies in continuous improvement of process efficiency and product quality (<a href="#_ENREF_10">Leit&atilde;o, Barbosa, Geraldes, &amp; Coelho, 2018</a>). …”
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Regulatory role of lnc-MAP3K13-3:1 on miR-6894-3p and SHROOM2 in modulating cellular dynamics in hepatocellular carcinoma by Kuai Chen, Manqin Zhu, Qinghua Hu, Hui Huang, Ka Chen, Xia Shuai, Jinshi Huang, Qiang Tao, Zhibin Guo

“…Methods Quantitative PCR (qPCR) was initially employed to measure the expression levels of lnc-MAP3K13-3:1 and miR-6894-3p in three HCC cell lines: HepG2, HuH-7, and Li-7. …”
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Syntactic Analysis of Spatial Structure Characteristics of Traditional Villages Along the Yellow River in Western Shanxi Province by Chenge GAO, Fang WANG

“…Finally, the K-prototype mixed clustering analysis method is employed to iteratively analyze the data on the global and local, as well as the foreground and background spatial structures of the 33 traditional village samples. …”
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Analysis of Factors Affecting Quality of Life (QoL) in Middle-Aged Stroke Patients (Under 65 Years Old) in the Rehabilitation Phase: Multivariate Regression Analysis by Lumbantobing VBM, Priastomo M, Kosasih CE, Rhamelani P, Anggadiredja K, Adnyana IK

“…Valentina Belinda M Lumbantobing,1,2,&amp;ast; Mukti Priastomo,1 Cecep Eli Kosasih,3 Putri Rhamelani,4 Kusnandar Anggadiredja,1 I Ketut Adnyana1,&amp;ast; 1Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, Institut Teknologi Bandung, Bandung, West Java, Indonesia; 2Department of Fundamental Nursing, Faculty of Nursing, Universitas Padjadjaran, Bandung, West Java, Indonesia; 3Department of Critical Nursing, Faculty of Nursing, Universitas Padjadjaran, Bandung, West Java, Indonesia; 4Faculty of Nursing, Universitas Padjadjaran, Sumedang, West Java, Indonesia&amp;ast;These authors contributed equally to this workCorrespondence: Valentina Belinda M Lumbantobing, Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, Institut Teknologi Bandung, Bandung, West Java, Indonesia, Email valentina@unpad.ac.id I Ketut Adnyana, Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, Institut Teknologi Bandung, Bandung, West Java, Indonesia, Email ketut@itb.ac.idPurpose: This study analyzed factors influencing the quality of life (QoL) in stroke patients during the rehabilitation phase so that rehabilitation professionals, caregivers, and stroke patients can pay attention to all aspects that contribute significantly to a better QoL and can design appropriate interventions to improve patient welfare and support a more significant recovery process.Patients and Methods: A non-experimental quantitative approach with a cross-sectional design was conducted with 76 adult stroke patients in the rehabilitation phase. …”
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Percepción de universitarios sobre la inclusión educativa en la Licenciatura en Cultura Física y Deporte (Perception of university students about educational inclusion in the degre... by Andrea Elisa Salas Hoyos, Javier Fernando Cuevas Rios, Graciela Hoyos Ruiz, María Elena Chávez Valenzuela, Edgardo Valenzuela Robles, Saul Ignacio Vega Orozco

“…Questions regarding University and Disability (CUNIDIS) scale were used to collect the information. The data analysis was done through the SPSS V.25 Program, with an alpha of 0.94 and a KMO- and Bartlett was obtained with a score of 0.84 and a significance of three decimal places. …”
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Deubiquitinating enzyme USP2 regulates brown adipose tissue thermogenesis via controlling EBF2 stabilization by Yuejie Xu, Ying Chen, Ningning Bai, Yingying Su, Yafen Ye, Rong Zhang, Ying Yang, Caizhi Liu, Cheng Hu, Jiemin Pan

“…The deubiquitinating enzyme family members are widely involved in the process of energy metabolism. However, the specific deubiquitinating enzyme member that affects the BAT thermogenesis remains largely unexplored. …”
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Implementing psychosocial guidelines into specialized spinal cord injury rehabilitation services to strengthen person-centred health care: protocol for a mixed methods study by Candice McBain, Candice McBain, Anne Marie Sarandrea, Anne Marie Sarandrea, Anne Marie Sarandrea, Ilaria Pozzato, Ilaria Pozzato, Mohit Arora, Mohit Arora, Daniel Myles, Daniel Myles, John Bourke, John Bourke, Yvonne Tran, Ian D. Cameron, Ian D. Cameron, James W. Middleton, James W. Middleton, James W. Middleton, Ashley Craig, Ashley Craig

“…Success of implementation will be determined by analyzing any shifts in perceived knowledge, attitudes, and behaviour of staff and cultural/structural processes observed through comparing baseline and post-intervention qualitative and quantitative data. …”
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Inflammatory markers after supplementation with marine n-3 or plant n-6 PUFAs: A randomized double-blind crossover study by Elise Grytten, Johnny Laupsa-Borge, Kaya Cetin, Pavol Bohov, Jan Erik Nordrehaug, Jon Skorve, Rolf K. Berge, Elin Strand, Bodil Bjørndal, Ottar K. Nygård, Espen Rostrup, Gunnar Mellgren, Simon N. Dankel

“…These data point to tissue-specific proinflammatory effects of high n-6 intake, but a net systemic anti-inflammatory effect as for n-3.…”
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Genome-wide identification of VOZ gene family in ten cotton species and the function analysis of GhVOZ2 involved in heat stress response by Xiangxiang Hu, Kailu Chen, Shuangquan Xie, Quanliang Xie, Xifeng Chen, Zhuang Meng, Asigul Ismayil, Xiang Jin, Shandang Shi, Fei Wang, Hongbin Li

“…Expression profile analysis of GhVOZ genes through both publicly available RNA-seq data from G. hirsutum and quantitative reverse transcription PCR (RT-qPCR) indicated that GhVOZ members are involved in tissue development and biotic and abiotic responses, especially GhVOZ2 showed continuous high expressions in different tissues and stress treatments, with the most significantly induced accumulation under heat stress. …”
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Quantum kernel t-distributed stochastic neighbor embedding by Yoshiaki Kawase, Kosuke Mitarai, Keisuke Fujii

“…In our numerical experiments, we visualize a hand-written digits data set and apply a k-nearest neighbor algorithm to the low-dimensional data to quantitatively evaluate our proposed method compared with a classical kernel method. …”
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Multi-institutional TSA-amplified Multiplexed Immunofluorescence Reproducibility Evaluation (MITRE) Study by Bernard A Fox, Chichung Wang, Kristin Roman, Carla Coltharp, Carmen Ballesteros-Merino, Shawn M Jensen, Janis M Taube, David L Rimm, Cliff Hoyt, Mei Jiang, Edwin R Parra, Ignacio Wistuba, Elizabeth L Engle, John McGuire, Bethany Remeniuk, Darren Locke

“…Standardization and validation of an end-to-end workflow that supports multisite trials and clinical laboratory processes are vital. Six institutions collaborated to: (1) optimize an automated six-plex assay focused on the PD-1/PD-L1 axis, (2) assess intersite and intrasite reproducibility of staining using a locked down image analysis algorithm to measure tumor cell and immune cell (IC) subset densities, %PD-L1 expression on tumor cells (TCs) and ICs, and PD-1/PD-L1 proximity assessments.Methods A six-plex mIF panel (PD-L1, PD-1, CD8, CD68, FOXP3, and CK) was rigorously optimized as determined by quantitative equivalence to immunohistochemistry (IHC) chromogenic assays. …”
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SGLT2 inhibitor downregulates ANGPTL4 to mitigate pathological aging of cardiomyocytes induced by type 2 diabetes by Yun Wen, Xiaofang Zhang, Han Liu, Haowen Ye, Ruxin Wang, Caixia Ma, Tianqi Duo, Jiaxin Wang, Xian Yang, Meixin Yu, Ying Wang, Liangyan Wu, Yongting Zhao, Lihong Wang

“…However, there are no reported data on their improvement of cardiomyocytes function through the alleviation of aging. …”
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A prática dos enfermeiros obstetras egressos da especialização da Escola de Enfermagem Anna Nery [The practice of obstetric nurses with a degree from the specialization course at A... by Bianca Dargam Gomes Vieira, Maria Aparecida Vasconcelos Moura, Valdecyr Herdy Alves, Diego Pereira Rodrigues

“…Data collection was conducted through semi-structured interviews from March to September, 2008. …”
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Perfil socioeconômico e cultural do estudante ingressante no curso de graduação em enfermagem [Social, economic, and cultural profile of incoming undergraduate students at the scho... by Norma Valéria Dantas de Oliveira Souza, Lucia Helena Garcia Penna, Luana dos Santos Cunha, Amanda de Almeida Sant’anna Baptista, Iraneide Ferreira Mafra, Débora Cristina de Almeida Mariano

“…Participants were students in their first school term of the undergraduate program in 2008/1, 2008/2, and 2009/1. Data were collected through questionnaires filled up by 97 students, who accounted for 80.80 % of the freshmen population, totaling 120 students. …”
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El estilo de vida del estudiantado de Pedagogía en Educación Física de una universidad del centro sur de Chile (The lifestyle of the Pedagogy in Physical Education students at a un... by Alex Garrido-Méndez, Carlos Matus Castillo, Catalina Pereira Araneda, Pablo Luna Villouta, Rafael Zapata Lamana, Oscar Andrades Ramírez, Carol Flores Rivera, Rodrigo Carrillo Monsalve, Carlos Celis Morales

“…The ESTIPENDO closed question survey was applied and was answered by 108 students from the previously mentioned academic program. Data analysis was done with SPSS v. 25 software. …”
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