Expressions of LncRNA HOTAIR and VEGF in adrenal malignant tumors and their prognostic significance by Du Xue-qian, Sun Qing-feng, Tian Long-jiang

“…Objective To explore the expression levels of long non-coding RNAHOX transcript antisense intergenic RNA（LncRNAHOTAIR）and vascular endothelial growth factor（VEGF）in patients with adrenal malignant tumor,and analyze their prognostic relationship.Methods From January 2014 to January 2017,167 cases of adrenal gland specimens were selected.The expression levels of HOTAIR and VEGF mRNA were detected by real-time fluorescent quantitative PCR（qRT-PCR）.Pearson’s method was employed for analyzing the correlation between HOTAIR and VEGF.And Kaplan-Meier method was applied for evaluatimg the relationship between HOTAIR,VEGF mRNA expression levels and survival of patients with adrenal malignant tumor.In addition,COX method was utilized for analyzing the prognostic factors of patients with adrenal malignant tumor.Results Compared with those in benign adrenal tissues,the relative expression levels of HOTAIR and VEGF mRNA were higher in malignant adrenal tumors（P<0.05）.HOTAIR was positively correlated with VEGF（r=0.739,P< 0.05）.The relationship between HOTAIR,VEGF mRNA and clinicopathological features of adrenal tumors showed that HOTAIR and VEGF mRNA were correlated with distant metastasis（P<0.05）,but not with age,gender,tumor diameter,single or multiple tumors（P>0.05）.The mean survival time of adrenal tumor patients with low HOTAIR expression was（32.09±0.92）months.It was significantly longer than（21.64±1.49）months of adrenal tumor patients with high HOTAIR expression（P<0.05）;The mean survival time of adrenal tumor patients with low VEGF mRNA expression was（31.81±0.97）months and it was significantly longer than（22.37±1.47）months of adrenal tumor patients with high VEGF mRNA expression（P<0.05）.Multivariate analysis indicated that distant metastasis,high expressions of HOTAIR and VEGF were independent risk factors for poor prognosis of patients with adrenal tumors.Conclusions The expressions of LncRNA HOTAIR and VEGF are up-regulated in adrenal malignant tumors.Both may be biomarkers of poor prognosis in patients with adrenal tumors.…”
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Identification and genetic characterization of Xiabuqu River virus: A novel member of the Iflaviridae family detected in soft ticks from Tibet, China by Meixi Ren, Mingli Sun, bataj Dan, Yingxin Tu, Guoyu Niu, Weiping Lin, Hengyi Sun, Yujing Zhu

“…Quantitative RT-PCR and nested PCR were utilized to investigate the distribution of XRV within the tick sample. …”
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Genome-wide sequencing of cellular microRNAs identifies a combinatorial expression signature diagnostic of sepsis. by Yuqian Ma, David Vilanova, Kerem Atalar, Olivier Delfour, Jonathan Edgeworth, Marlies Ostermann, Maria Hernandez-Fuentes, Sandrine Razafimahatratra, Bernard Michot, David H Persing, Ingrid Ziegler, Bianca Törös, Paula Mölling, Per Olcén, Richard Beale, Graham M Lord

“…MicroRNAs are small non-coding RNAs that play an important role in the regulation of numerous cellular processes, including inflammation and immunity.…”
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Education of medical caregivers in Poland: areas requiring improvement – mixed-method research by Magdalena Krysińska-Pisarek, Katarzyna Domosławska-Żylińska, Piotr Tyszko

“…Coding disagreements were resolved through discussion among the three researchers until consensus was reached. …”
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Evolutionary adaptations of cyanobacterial polyhydroxybutyrate (PHB) biosynthesis and metabolic pathways in Spirulina, Arthrospira, and Limnospira spp by Fayaazuddin Thajuddin, Asraf Sithikka Rasheed, Prakash Palanivel, Shakena Fathima Thajuddin, Thajuddin Nooruddin, Dhanasekaran Dharumadurai

“…This study focuses on PHB production in the cyanobacteria, Limnospira fusiformis NRMCF6962 and Spirulina major NRMCF6963, under nitrogen-limited conditions. Qualitative and quantitative analyses confirmed PHB accumulation in L. fusiformis and its absence in S. major. …”
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Building Bobcat Bounty: The Design, Implementation, and Initial Evaluation of a Student-Led Food Pantry to Address College Student Food Insecurity by Lesli Biediger-Friedman, Cassandra M. Johnson, Hannah Thornton, Marissa Buckley

“…Descriptive statistics and t-tests were used for quantitative analyses, and qualitative analyses included interview transcript coding and peer debriefing. …”
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The lncRNA MAGI2-AS3 in peripheral blood mononuclear cells: a valuable biomarker for diagnosis and prognosis prediction of breast cancer by Shanmei Du, Hong Yang, Yong Yang, Kui Liu

“…Background Existing research has shown that long non-coding RNA (lncRNA) MAGI2 antisense RNA3 (MAGI2-AS3) expression is significantly decreased in breast cancer tissues and can inhibit breast cancer progression. …”
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Interdisciplinary medication reviews of psychiatric patients – A mixed method evaluation by Dagmar Abelone Dalin, Sara Sommer Holst, Lucif Søemosegaard Dalin, Charlotte Vermehren

“…The study was a mixed-method evaluation study that included 11 quantitative MRs analyzed by descriptive analysis and 5 qualitative semi-structured interviews analyzed by thematic coding analysis. …”
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Structural Challenges of Attracting and Retaining Sponsorship in Professional Sports by Somayeh Mohammadi, Behzad Izadi

“…A purposeful sampling method was utilized, and in-depth interviews were conducted with 24 participants until theoretical saturation was reached. The coding technique and NVIVO software were used for data analysis. …”
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LncRNA GTF3C1 promotes diabetic corneal wound healing by regulating GABARAP and PTEN to augment autophagy by Danling Liao, Wenqu Chen, Yuyang Deng, Shijia Wei, Li Wang, Jianzhang Hu

“…Autophagy activity, corneal nerve density, and epithelial healing were quantified using quantitative real-time polymerase chain reaction (qRT-PCR), immunofluorescence, and histology in diabetic models. …”
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A virtual-reality (VR) cognitive pupillometry analysis of auditory and visual phonemic awareness tasks involving ‘th’ sound variations by Mohsen Mahmoudi-Dehaki, Nasim Nasr-Esfahani

“…Rooted in the Cognitive Load (CL) and Dual Coding theories, this sequential explanatory study examined the CL levels experienced by 36 available Iranian pre-intermediate English as a Foreign Language (EFL) learners in auditory and visual Phonemic Awareness Tasks (PATs). …”
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Turnover intention among intensive care nurses and the influence of the COVID-19 pandemic: a scoping review by Tanja Lesnik, Anna Hauser-Oppelmayer

“…The factors were qualitatively coded in MAXQDA, resulting in an inductive coding frame. …”
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Episodic sexual transmission of HIV revealed by molecular phylodynamics. by Fraser Lewis, Gareth J Hughes, Andrew Rambaut, Anton Pozniak, Andrew J Leigh Brown

“…<h4>Methods and findings</h4>Sequences of the protease and reverse transcriptase coding regions from 2,126 patients, predominantly MSM, from London were compared: 402 of these showed a close match to at least one other subtype B sequence. …”
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Advances in the molecular diagnostic methods for circular RNA by Zequan Ye, Ke Wang, Jianhua Zhou, Jiasi Wang

“…Circular RNA (circRNA) is a unique type of non-coding RNA, which is produced by back splicing of mRNA to form the covalent junction without 3′-5′ polarity and poly(A) tail. …”
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Analysis of MicroRNA -155-5p Expression in Patients with Primary Myelofibrosis. by Sarah I. Khaleel, Jaffar N. AlAlsaidissa

“…MicroRNAs (miRNAs) are short, non-protein-coding RNA molecules, typically 18–24 nucleotides in length. …”
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Evaluating factors that impact scoring an open response situational judgment test: a mixed methods approach by Muhammad Zafar Iqbal, Rodica Ivan, Colleen Robb, Jillian Derby

“…All interviews were transcribed verbatim, which were then thematically analyzed using an inductive coding technique.ResultsResults from both the content analyses and think-aloud activities revealed that several construct relevant factors influenced scores. …”
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Genome-wide identification and characterization of DNA enhancers with a stacked multivariate fusion framework. by Yansong Wang, Zilong Hou, Yuning Yang, Ka-Chun Wong, Xiangtao Li

“…Enhancers are short non-coding DNA sequences outside of the target promoter regions that can be bound by specific proteins to increase a gene's transcriptional activity, which has a crucial role in the spatiotemporal and quantitative regulation of gene expression. …”
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Shrinking Cities of the Russian Arctic: Public Discourse of Vorkuta Residents on the Place Identity and the Reasons for Population Outflow by Elena V. Nedoseka, Ekaterina N. Sharova, Veronika A. Lisova

“…Using open and axial coding techniques, the dominant subdiscourses in the perception of place identity and a group of urban development problems that cause the outflow of population from the city were meaningfully identified. …”
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Presenting a Model of Managerial practical wisdom in Hospitals by Mahdavi Akramsadat, Ardabili Farzad Sattari, Kheirandish Mohammad, Ebrahimpour Habib, Daryani Shahram Mirzaei

“…These results can be used in the creation and development of quantitative tools for measuring managerial practical wisdom and as a qualitative complement in evaluating the quality of decisions among hospital managers.…”
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A comparison of the expression patterns and diagnostic capability of the ncRNAs NEAT1 and miR-34a in non-obstructive azoospermia and severe oligospermia by Aya Salman, Abdullah F. Radwan, Olfat G. Shaker, Adel A., Ghadir A. Sayed

“…The interactions between these non-coding RNA (ncRNAs) were explored, along with their correlations to hormonal profiles and clinical parameters like sperm count and motility. …”
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