High-resolution deep mutational scanning of the melanocortin-4 receptor enables target characterization for drug discovery by Conor J Howard, Nathan S Abell, Beatriz A Osuna, Eric M Jones, Leon Y Chan, Henry Chan, Dean R Artis, Jonathan B Asfaha, Joshua S Bloom, Aaron R Cooper, Andrew Liao, Eden Mahdavi, Nabil Mohammed, Alan L Su, Giselle A Uribe, Sriram Kosuri, Diane E Dickel, Nathan B Lubock

“…Deep Mutational Scanning (DMS) is an emerging method to systematically test the functional consequences of thousands of sequence changes to a protein target in a single experiment. …”
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Management and Prognosis of Head and Neck Cancer Patients during the COVID-19 Pandemic by Giuliano Lozer Bruneli, Priscila Marinho de Abreu, Jhully Oliveira Ferreira, Camila Batista Daniel, Jéssica Graça Sant'Anna, Roberta Ferreira Ventura Mendes, Brena Ramos Athaydes, Mariane Vedovatti Monfardini Sagrillo, José Roberto Vasconcelos de Podestá, Marco Homero de Sá Santos, Jeferson Lenzi, Evandro Duccini de Souza, Ricardo Mai Rocha, Regina de Pinho Keller, Liliana Cruz Spano, Sandra Ventorin von Zeidler

“…Objective: To evaluate the measures adopted by an oncological hub during the COVID-19 pandemic and investigate whether the SARS-CoV-2 infection and viral load changed the outcomes of head and neck cancer patients. …”
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Mitochondrial metabolism is rapidly re-activated in mature neutrophils to support stimulation-induced response by Jorgo Lika, Jorgo Lika, James A. Votava, Rupsa Datta, Carlos A. Mellado Fritz, Carlos A. Mellado Fritz, Aleksandr M. Kralovec, Aleksandr M. Kralovec, Frances M. Smith, Anna Huttenlocher, Anna Huttenlocher, Melissa C. Skala, Melissa C. Skala, Jing Fan, Jing Fan, Jing Fan

“…Treatment with these inhibitors also impacts neutrophil redox status, migration, and apoptosis without significantly changing overall bioenergetics.ConclusionsTogether, these results demonstrate that mitochondrial metabolism is dynamically remodeled and plays a significant role in neutrophils. …”
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INFLUENCE OF COMPLETENESS HEART REVASCULARIZATION ON A FUNCTIONAL CONDITION OF MYOCARDIUM AT ISCHEMIC CARDIOMYOPATHY by V. V. Chestukhin, A. B. Mironkov, F. A. Blyakhman, E. N. Ostroumov, S. G. Kolchanova, T. F. Shklyar, E. T. Asoev, S. A. Sakhovsky

“…By means of echocardiography and quantitative gated SPECT estimated dynamics of systolic and diastolic function, change of perfusion, thickening and myocardial movement. …”
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Explaining the contrast responses of evergreen needle-leaved forest and grassland carbon fluxes during the growing season droughts over the North Temperate Zone by Xiaoyuan Li, Shanlei Sun, Yongjian Ren, Zaoying Bi, Yi Liu, Yibo Liu, Botao Zhou, Haishan Chen

“…Therefore, it is crucial to quantitatively assess how drought influences carbon fluxes in these ecosystems. …”
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Myocardial-to-background ratio in F18-fluorodeoxyglucose PET in cardiac sarcoidosis for diagnosis and evaluation of therapeutic response by Laura Ueberham, Theresa Kluge, Konrad Latuscynski, Hans Ebbinghaus, Joanna Jozwiak-Nozdrzykowska, Stephan Renziehausen, Annika Reuser, Kerstin Bode, Regine Kluge, Ulrich Laufs, Osama Sabri, Borislav Dinov

“…Objectives: FDG uptake parameters were investigated semi-quantitatively in biopsy-proven CS at time of diagnosis (CSD) and during immunosuppressive therapy (CST) and in controls. …”
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Novel Dimethylacetamide-Containing Formulation Improves Infraorbital Anaesthesia Efficacy in Rats with Periodontitis by Ekaterina V. Blinova, Evgeniia V. Shikh, Elena V. Semeleva, Aleksandra M. Yurochkina, Andrey V. Novikov, Anna P. Vediaeva, Arkadii B. Lebedev, Elena G. Lobanova, Olga V. Vasilkina, Dmitry S. Blinov, Yan A. Mazov, Evgeniia A. Kogan

“…Tissue IL-1β, IL-10, and TNF-α concentration was quantitatively assessed by an enzyme-linked immunosorbent assay. …”
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Transcriptomic profiling of cytomegalovirus infection in cardiac transplantation: proof-of-concept for a new strategy in tissue markers application by Ilaria Barison, Diego Perazzolo, Chiara Castellani, Alessia Giarraputo, Elisabetta Rossi, Elisabetta Rossi, Luca Vedovelli, Sonia Anna Minuzzo, Chiara Tessari, Nicola Pradegan, Giuseppe Toscano, Francesco Tona, Cristina Basso, Gino Gerosa, Susanna Mandruzzato, Susanna Mandruzzato, Davide Abate, Dario Gregori, Annalisa Angelini, Marny Fedrigo

“…The future combined analysis of these genes and these miRNAs through user-friendly techniques, such as quantitative PCR, could reduce turn-around time and improve our diagnostic power for distinguishing CMV infection from ACR in EMB specimens.…”
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Research and application of image recognition-based identification for flesh browning of loquat fruits by CHEN Yujia, DENG Chaojun, ZHANG Tingting, WANG Xiuping, CHEN Xiuping, ZHAO Jianing, MA Cuilan, JIANG Jimou

“…[Conclusion] The MATLAB image segmentation algorithm had a wide recognition range and fast computational speed, which would be suitable for quantitative analysis of the color change process of intensive resources. …”
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Multi-modal MRI for objective diagnosis and outcome prediction in depression by Jesper Pilmeyer, Rolf Lamerichs, Sjir Schielen, Faroeq Ramsaransing, Vivianne van Kranen-Mastenbroek, Jacobus F.A. Jansen, Marcel Breeuwer, Svitlana Zinger

“…Research Purpose: The low treatment effectiveness in major depressive disorder (MDD) may be caused by the subjectiveness in clinical examination and the lack of quantitative tests. Objective biomarkers derived from magnetic resonance imaging (MRI) may support clinical experts during decision-making. …”
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Structural analyses and photovoltaic device simulations in two and three dimensions applied to Ag-alloyed Cu(In,Ga)Se2 solar cells by Chang-Yun Song, Zelong Wang, Matthias Maiberg, Heiko Kempa, Julia Horstmann, Wolfram Witte, Rico Gutzler, Dimitrios Hariskos, Daniel Abou-Ras, Sinju Thomas, Roland Scheer, Ali Gholinia

“…The present study reports on the structural changes and related photovoltaic properties resulting from the addition of Ag in Cu(In,Ga)Se _2 (CIGS)-based thin-film solar cells analysed using two-dimensional (2D) and three-dimensional (3D) analytical and modelling techniques. …”
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Investigating the nutritional status, outcomes and needs of adolescents and young adults (AYA) following a cancer diagnosis: protocol for a mixed-methods study by Andrew Murnane, Erin Laing, Belinda Steer, Jeremy Lewin, Heather Gilbertson, Elizabeth Mount, Mary-Anne Silvers, Jenelle Loeliger, Jodie Bartle, Kristin Mellett, June Savva, Pasquale L. Fedele, Lisa M. Orme, Leanne Super, Karla Gough, Kate Thompson

“…Methods and analysis The AYCANN-study (adolescent and young adult cancer nutrition project) adopts a mixed-methods study design, conducted over three inter-related studies; (1) Prospective observational study of AYA between 15 and 25 years, following diagnosis of any cancer type, to quantitatively assess changes in body weight, muscle mass/function, nutritional status, and health related quality of life (HRQoL). …”
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Understanding the effectiveness and underlying mechanisms of lifestyle modification interventions in adults with learning disabilities: a mixed-methods systematic review by Dikshyanta Rana, Sophie Westrop, Nishant Jaiswal, Evi Germeni, Arlene McGarty, Louisa Ells, Phillippa Lally, Michael McEwan, Craig Melville, Leanne Harris, Olivia Wu

“…Our meta-analysis was conducted on weight management outcomes: change in weight, change in body mass index (BMI), change in waist circumference and change in body fat. …”
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The effects of hypertensive disorders of pregnancy on fetal myocardial remodelling and cardiac function assessed by two-dimensional speckle tracking imaging by Yanhong Zhang, Lisi Zhang, Congxin Sun, Guihong Chen, Wei Zhao, Na Li, Jing Ma, Jianli Xue, Pin Wang, Wei Xiang, Shuping Ge, Yanju Wang

“…Two-dimensional speckle tracking technology offers the potential to provide more detailed information for the quantitative assessment of fetal size, morphology and cardiac function.…”
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Spatial transcriptomic and morpho-functional information derived from single mouse FFPE slides allows in-depth fingerprinting of lung fibrosis by Erica Ferrini, Costanza Bonfini, Giovanna Marchese, Martina Buccardi, Matteo Zoboli, Primetta Faccioli, Nicola Sverzellati, Gino Villetti, Simone Ottonello, Maria Ravo, Franco F. Stellari

“…For each sample, Pearson correlation analysis was used to compare expression levels of individual gene clusters with Ashcroft Scores and aeration compartments quantitatively assessed on the matched 2D micro-CT coronal slice. …”
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Stabilization and Solidification of Produced Tailings in the Lead Extraction Process Using Brine Leaching Method by Ravanbakhsh Shirdam, Atefeh Nezami

“…Notably, the slope of changes in compressive strength per cement was substantially higher for the 28-day samples compared to the 7-day samples. …”
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Struktur Mikroanatomi Hati dan Kadar Kolesterol Total Plasma Darah Tikus Putih Strain Wistar Pasca Suplementasi Minyak Lemuru dan Minyak Sawit by Ngudy Jaka Surasa, Nur Rahayu Utami, Wiwi Isnaeni

“…Blood plasma levels of total cholesterol, body weight of rats and mice liver weight quantitatively analyzed using one-way ANOVA, with an error rate of 5%. …”
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The Dynamics of Shannon Entropy in Analyzing Climate Variability for Modeling Temperature and Precipitation Uncertainty in Poland by Bernard Twaróg

“…The aim of this study is to quantitatively analyze the long-term climate variability in Poland during the period 1901–2010, using Shannon entropy as a measure of uncertainty and complexity within the atmospheric system. …”
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Research on Atlantic surface pCO2 reconstruction based on machine learning by Jiaming Liu, Jie Wang, Xun Wang, Yixuan Zhou, Runbin Hu, Haiyang Zhang

“…This indicates that the model can accommodate environmental data errors induced by abrupt changes in marine environments. Such robustness enhances its reliability in sea surface pCO2 reconstruction. …”
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High-fat diet mouse model receiving L-glucose supplementations propagates liver injury by Johnny Amer, Athar Amleh, Ahmad Salhab, Yuval Kolodny, Shira Yochelis, Baker Saffouri, Yossi Paltiel, Rifaat Safadi

“…We aimed to evaluate the effects of manufactured sugars (L-glucose) compared to natural sugars (D-glucose) on phenotype, molecular and metabolic changes in mice models fed with either regular diet (RD) or high fat diet (HFD).MethodsC57BL/6 mice fed 16-weeks with either RD; 70% carbohydrate or HFD; 60% fat, with or without additional glucose (Glu, at 18% w/v) to drinking tap water at weeks 8–16; of either natural (D-Glu) or manufactured (L-Glu) sugars. …”
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