Establishment and Application of TaqMan qPCR Detection Method for Human DNA Contamination in DNA Laboratory by SHEN Gao-fang, ZHOU Yong-song, ZHANG Jian-qiu, JI Shi-you, WU Ying-feng, SHANG Hao, ZHU Bo-feng

“…The correlation coefficient of the qPCR system was -0.999, and amplification efficiency was 100%. Both the intra-batch and inter-batch variation coefficients were less than 2%.ConclusionThe established human DNA detection method based on qPCR technique has good specificity, high sensitivity, and robust stability. …”
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Accelerated design of Tm3+-doped multicomponent germanate laser glasses via neighboring glassy compounds model by Jiahong Li, Haolin Yang, Zhenjie Lun, Zhongming Wen, Junyan Lu, Guowu Tang, Minbo Wu, Xiangyang Song, Qi Qian

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Spatiotemporal Decoupling Between Intensive Policy Interventions and Natural Gradual Recovery in Human–Land Systems During Transition Periods: A Remote Sensing Analysis by Xiao Xie, Chunxiao Zhang, Haojie Kang, Chao E, Jingzhong Li, Wanxia Ren

“…This decoupling effect between anthropogenic and natural drivers provides quantitative evidence for optimizing spatial targeting of ecological engineering and synchronizing restoration chrono sequences.…”
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REVISITING JUSTIFICATION OF TECHNO-ECONOMIC LEVEL OF AGRICULTURAL MACHINES AND EQUIPMENT by A. Yu. Izmailov, V. A. Makarov

“…The authors outline ways to improve efficiency of agricultural production with use of techno-economic indicators system aimed at machinery improving. …”
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Methodology for assessing the social effectiveness of exhibition activity by Yu. V. Vorontsova, D. S. Garbuz

“…The article presents the methodology developed by the authors for the quantitative assessment of social efficiency, which is complex and also includes the calculation of the selected elements’ sensitivity of social efficiency to different changes. …”
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Predictive channel scheduling algorithm between macro base station and micro base station group by Yinghai XIE, Ruohe YAO, Bin WU

“…A novel predictive channel scheduling algorithm was proposed for non-real-time traffic transmission between macro-base stations and micro-base stations in 5G ultra-cellular networks.First,based on the stochastic stationary process characteristics of wireless channels between stationary communication agents,a discrete channel state probability space was established for the scheduling process from the perspective of classical probability theory,and the event domain was segmented.Then,the efficient scheduling of multi-user,multi-non-real-time services was realized by probability numerical calculation of each event domain.The theoretical analysis and simulation results show that the algorithm has low computational complexity.Compared with other classical scheduling algorithms,the new algorithm can optimize traffic transmission in a longer time dimension,approximate the maximum signal-to-noise ratio algorithm in throughput performance,and increase system throughput by about 14% under heavy load.At the same time,the new algorithm is accurate.Quantitative computation achieves a self-adaption match between the expected traffic rate and the actual scheduling rate.…”
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New strategies of beneficial development of in-situ combustion in nearly abandoned heavy oil reservoirs by ZHENG Yiqiong, ZHANG Tao, LIU Haiying, RUAN Conghui, ZOU Shuai

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Leveraging environmental DNA (eDNA) to optimize targeted removal of invasive fishes by Jennie J. Wiggins, Vanessa D. Tobias, Erika F. Holcombe, Katie Karpenko, Eric R. Huber, Andrew C. Goodman

“…Our results indicate that aquatic invasive species removal is more efficient when eDNA detection techniques are combined with traditional trapping methods.…”
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Quantifying soil wind erosion attribution in Inner Mongolia’s desert grassland by Hongtao Jiang, Wenbang Gao, Baoyuan Liu, An Chang, Qiankun Guo

“…This study provides important theoretical support for regional wind erosion control and proposes targeted management strategies to improve management efficiency and promote ecological protection and sustainable development.…”
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Development of A Real-Time PCR Assay for Plasmodiophora brassicae and Its Detection in Soil Samples by Jin-ping LI, Yan LI, Yan-xia SHI, Xue-wen XIE, Chai A-li, Bao-ju LI

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Development of a one-step multiplex RT-qPCR method for rapid detection of bovine diarrhea viruses by Dequan Yang, Li Ma, Zhongping Yang, Xianchao Yang, Jian Wang, Houbin Ju, Chunguang Lu, Yonggang Weng, Heping Zhao, Haixiao Shen, Xin Li, Feifei Ge, Xiaoxu Wang, Xiujuan Wu, Meng Xiang, Guidan Feng, Congsheng Tang, Shixin Huang, Hongjin Zhao

“…Furthermore, the assay exhibited excellent reproducibility, with coefficients of variation below 1.5%, a strong linear correlation (R2 > 0.996), and an amplification efficiency between 90% and 110%. Validation with 80 clinical samples from both diarrheic and non-diarrheic cattle across four farms in Shanghai showed a high degree of concordance with RT-PCR, with positive detection rates for BKoV, BoAstV, and BToV at 28.75%, 8.75%, and 3.75%, respectively, highlighting the predominance of BKoV and BoAstV. …”
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A streamlined POCT solution for rapid infectious disease detection by Yaping Xie, Yan Yu, Shuaiwu Huang, Liangcheng Wan, Chang Wu, Chiwei Yin, Jianjun Li, Jiangang Ling, Lizhong Dai

“…This study presents iPonatic, an integrated POCT system with a quantitative dropper for efficient sample addition, a low-cost cartridge utilizing one-step rapid nucleic acid release technology, and real-time data synchronization via a cloud platform. …”
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High-throughput phenotyping in grapevine breeding research: technologies and applications by Katja Herzog, Anna Kicherer, Nagarjun Malagol, Oliver Trapp, Reinhard Töpfer

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One-step and two-step qPCR assays for CAPRV2023: development and application in full-cycle epidemiological surveillance of golden pompano by Heng Sun, Heng Sun, Bissih Fred, Bissih Fred, Haoyu Wang, Haoyu Wang, Jie Huang, Jie Huang, Zihao Wu, Zihao Wu, Dandan Wu, Dandan Wu, Yishan Lu, Yishan Lu, Jichang Jian, Jichang Jian, Yucong Huang, Yucong Huang

“…It achieved a detection limit of 15 copies/μL, with a high amplification efficiency (102.8%) and excellent repeatability (CV = 0.81%). …”
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Evaluating Geostatistical and Statistical Merging Methods for Radar–Gauge Rainfall Integration: A Multi-Method Comparative Study by Xuan-Hien Le, Naoki Koyama, Kei Kikuchi, Yoshihisa Yamanouchi, Akiyoshi Fukaya, Tadashi Yamada

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Unveiling genetic basis of seedling emergence from deep soil depth under dry direct- seeded conditions in rice (Oryza sativa L.) by Vagish Mishra, Vagish Mishra, Shilpi Dixit, Shilpi Dixit, Swati Tyagi, Swati Tyagi, Challa Venkateswarlu, Challa Venkateswarlu, Pronob J. Paul, Pronob J. Paul, Anoop Kishor Singh Gurjar, Anoop Kishor Singh Gurjar, Shalabh Dixit, Nitika Sandhu, Smita Kurup, Arvind Kumar, Arvind Kumar, Pallavi Sinha, Pallavi Sinha, Vikas Kumar Singh, Vikas Kumar Singh, Uma Maheshwar Singh, Uma Maheshwar Singh

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WaveConv-sLSTM-KET: A Novel Framework for the Multi-Task Analysis of Oil Spill Fluorescence Spectra by Shubo Zhang, Menghan Li, Jing Li

“…The frequent occurrence of marine oil spills underscores the need for efficient methods to identify spilled substances and analyze their thickness. …”
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Capturing the extent of climate's dynamic effects on runoff and nutrient yields across short- and long-term scales by Di Chang, Shuo Li

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Research on the optimization model of anti-breast cancer candidate drugs based on machine learning by Zhou Dong, Hong Chen, Yuchen Yang, Hairong Hao

“…This multi-objective optimization model provides a novel and efficient tool for drug development, offering significant improvements in both biological activity and pharmacokinetic properties, with practical implications for the optimization of future anti-breast cancer drugs.…”
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The Elemental Analysis of Deep Earth Cuttings Based on Laser-Induced Breakdown Spectroscopy by GUO Qingbin, HU Wei, SHI Mingxin, ZHENG Pengfei, WU Di, AI Yong, CHEN Minxin, YU Jun

“…To address the demand for rapid in-situ detection of mineral and carbon elements in cuttings during ultra-deep hydrocarbon exploration, this study proposes an efficient elemental composition detection method based on laser-induced breakdown spectroscopy (LIBS). …”
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