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    Novel duplex TaqMan-based quantitative PCR for rapid and accurate diagnosis of Leishmania (Mundinia) martiniquensis and Leishmania (Mundinia) orientalis, responsible for autochthon... by Kanok Preativatanyou, Nopporn Songumpai, Pathamet Khositharattanakool, Rinnara Ampol, Chulaluk Promrangsee, Chatchapon Sricharoensuk, Kobpat Phadungsaksawasdi, Thanapat Pataradool, Tomas Becvar, Barbora Vojtkova, Petr Volf, Padet Siriyasatien

    Published 2024-01-01
    “…Standard curves of the singleplex and duplex assays showed good linearity with excellent amplification efficiency. Using conventional ITS1-PCR and plasmid sequencing as a reference standard assay, the duplex qPCR showed diagnostic sensitivity and specificity of 100% and positive and negative predictive values of 100% for both Leishmania species with a perfect level of agreement (kappa = 1.0). …”
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  4. 944

    Environmental DNA Detects Endangered Texas Hornshell and Its Hosts by Daniel H. Mason, Heidi A. Henderson, Thomas W. Franklin, Michael K. Young, Jennifer E. Hernandez, Cory L. Engkjer, Joanna Hatt, Michael K. Schwartz

    Published 2025-07-01
    “…ABSTRACT Environmental DNA techniques are continuously demonstrating their efficiencies over traditional survey methods for detecting rare species. …”
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  5. 945

    Validation of quantitative real-time PCR reference genes for the determination of seasonal and labor-specific gene expression profiles in the head of Western honey bee, Apis mellif... by KyungHwan Moon, Si Hyeock Lee, Young Ho Kim

    Published 2018-01-01
    “…To this end, in an attempt to search for reliable reference genes, the amplification efficiencies of six candidate reference genes (rp49, rpL32, rpS18, tbp, tub, and gapdh) were determined. …”
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  6. 946

    Quantitative evaluation system of geothermal resources based on analytic hierarchy process: A case study of middle-deep hydrothermal sandstone reservoir in Caofeidian of Hebei Prov... by HE Dongbo, REN Lu, HAO Jie, LIU Xiaoping, CAO Qian

    Published 2023-12-01
    “…Level Ⅰ areas are resource advantage areas that can be efficiently developed; Level Ⅱ areas are resource rich areas that meet the requirements of industrial development; Level Ⅲ areas are resource non enriched areas. …”
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    Quantitative characterization of the multiscale mechanical properties of low-permeability sandstone roofs of coal seams based on nanoindentation and triaxial tests and its implicat... by Feng Cao, Jianhua He, Hongxiu Cao, Hucheng Deng, Andrew D. La Croix, Rui Jiang, Ruixue Li, Jiarun Li

    Published 2025-01-01
    “…The major conclusion is that the positive rhythm lithofacies of medium-grained sandstone to siltstone are the most favorable for efficient CO2 sequestration in deep unmineable coal seams.…”
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    CADA: A Flexible and Elastic DDoS Mitigation Architecture by Junrui Wu, Zhige He, Wenyong Wang, Haoran Luo, Shuisong Hu, Yu Miao, Yubo Li, Jun Deng

    Published 2025-01-01
    “…The selection of deployment strategies affects the efficiency of CADA’s DDoS mitigation. Therefore, we propose a genetic algorithm-based quantitative deployment algorithm that generates deployment strategies for each service chain through a fitness function that quantifies response time and resource utilization. …”
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    Towards eco-metabolomics: NADES-guided extraction enables semi-quantitative metabolomics for Melissa officinalisThe MZmine batch files have been uploaded to Zenodo for accessibilit... by Chiara Spaggiari, Isa Sara Aimee Hiemstra, Antoinette Kazbar, Gabriele Costantino, Laura Righetti

    Published 2025-02-01
    “…Interestingly, thymol-menthol NADES demonstrated the ability to efficiently extract a broad range of bioactive compounds, yielding a metabolome comparable to that obtained with conventional ethanolic. …”
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  15. 955

    <i>Megaselia scalaris</i> and <i>Senotainia tricuspis</i> Infesting <i>Apis mellifera</i>: Detection by Quantitative PCR, Genotyping, and Involvement in the Transmission of Microbi... by Franca Rossi, Martina Iannitto, Beqe Hulaj, Paola Manocchio, Francesca Gentile, Ilaria Del Matto, Massimiliano Paoletti, Lucio Marino, Luciano Ricchiuti

    Published 2024-10-01
    “…The results indicated that these parasites should be efficiently controlled in apiaries by using rapid detection methods to facilitate the large screening studies and early detection.…”
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    Experimental Study of Confining Pressure-Induced Fracture Network for Shale Gas Reservoir Under Triaxial Compression Conditions by Jinxuan Han, Ming Gao, Yubo Wu, Ali Raza, Pei He, Jianhui Li, Yanjun Lu, Manping Yang, Hongjian Zhu

    Published 2025-05-01
    “…We suggest that the bedding plane gap compression, radial deformation limitation, and interlayer effect weakening are efficient mechanisms for the formation of shale fracture networks induced by confining pressure, and that confining pressure plays a significant role in limiting and weakening the development of shale fractures, based on the quantitative characterization results of fractures.…”
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    Chromato-mass spectroscopy of tinctures from the underground part of Valerian plants growing in Zaporizhzhia region by V. M. Odyntsova, Yu. I. Korniievskyi, D. Yu. Skoryna, V. Н. Korniievska

    Published 2022-12-01
    “…In the 18th century, valerian was included in all European pharmacopeias. High efficiency, good tolerability, and virtually no side effects ensure the widespread use of valerian and its phytopreparations in medical practice, especially for the treatment of geriatric patients. …”
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    Quantifying Tree Structural Change in an African Savanna by Utilizing Multi-Temporal TLS Data by Tasiyiwa Priscilla Muumbe, Jussi Baade, Pasi Raumonen, Corli Coetsee, Jenia Singh, Christiane Schmullius

    Published 2025-02-01
    “…Given this complexity, it is essential to monitor and quantify woody structural changes in savannas efficiently. We implemented a non-destructive approach based on Terrestrial Laser Scanning (TLS) and Quantitative Structure Models (QSMs) that offers the unique advantage of investigating changes in complex tree parameters, such as volume and branch length parameters that have not been previously reported for savanna trees. …”
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