Instantaneous dose rate as a crucial factor in reducing mortality and normal tissue toxicities in murine total-body irradiation: a comparative study of dose rate combinations by Hongyu Zhu, Shihua Liu, Jiaqi Qiu, Ankang Hu, Wanyi Zhou, Jian Wang, Weihang Gu, Yinuo Zhu, Hao Zha, Rong Xiang, Junli Li, Rui Qiu, Chong Zhao, Peng Huang, Xiaowu Deng

“…Mice were euthanized at 3 h and 48 h post irradiation to assess acute normal tissue damage and perform transcriptome sequencing. …”
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Mesenchymal stromal cells conditioned by peripheral blood mononuclear cells exert enhanced immunomodulation capacities and alleviate a model of Myasthenia Gravis by Alexandra C. Bayer, Natalia Pinzón, Axel You, Cinthia Bergman, Nadine Dragin, Aurélien Corneau, Frédérique Truffault, Danièle Noël, Christophe Martinaud, Rozen Le Panse, Sonia Berrih-Aknin, Jean-Thomas Vilquin

“…Comparatively, 2089 genes were differentially expressed between rMSC and γMSC, highlighting host defense, anti-viral response, NFκB signaling pathways modulated by IFN-γ. …”
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The developmental toxicity of bisphenol F exposure on the zebrafish larvae by Biyu Wu, Zirui Cheng, Xiang Li, Minxing Liang, Xue Wang, Duan Pi, Jiayi Liu, Huiling Li, Jun Zhao, Junjie Wang, Fang Liang, Xuegeng Wang

“…To explore the underlying mechanisms, transcriptomic analysis was performed to identify differentially expressed genes (DEGs). …”
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Mast cell activation may contribute to adverse health transitions in COVID-19 patients with frailty by Xiangqi Li, Chaobao Zhang, Zhijun Bao

“…The results revealed that patients with frailty were more prone to poor health conversions and more sequelae, and the blood transcriptome had obvious disturbances in pathways associated with immune regulation, metabolism, and stress response. …”
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Acute blood loss in mice forces differentiation of both CD45-positive and CD45-negative erythroid cells and leads to a decreased CCL3 chemokine production by bone marrow erythroid... by Kirill Nazarov, Roman Perik-Zavodskii, Olga Perik-Zavodskaia, Saleh Alrhmoun, Marina Volynets, Julia Shevchenko, Sergey Sennikov

“…In this study, we studied the immunological response of murine erythroid cells to acute blood loss using flow cytometry, NanoString immune transcriptome profiling, and BioPlex cytokine secretome profiling. …”
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<i>Colletotrichum gloeosporioides</i> Swiftly Manipulates the Transcriptional Regulation in <i>Citrus sinensis</i> During the Early Infection Stage by Siyu Zhang, Xinyou Wang, Wei Zeng, Leijian Zhong, Xiaoyong Yuan, Zhigang Ouyang, Ruimin Li

“…Upon inoculation with <i>C. gloeosporioides</i>, there were 4600 differentially expressed genes (DEGs) with 1754 down-regulated and 2846 up-regulated after six hours, while there were only 580 DEGs with 185 down-regulated and 395 up-regulated between six and twelve-hours post-inoculation. Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes enrichment analysis indicated that the DEGs, which exhibited consistent up-regulation, were associated with metabolic processes and stress responses. …”
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Arginine methylation modulates tumor fate and prognosis in clear cell renal cell carcinoma by Jiahao Wang, Dan Bao, Xiaochao Chen, Zijie Yu, Weiyu Kong, Chen Xu, Songtao Li, Yulin Yue

“…Abstract Background Arginine methylation, a key post-translational modification, plays a pivotal role in regulating various cellular processes and has been implicated in cancer progression. …”
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In ovo delivery of carvacrol triggers expression of chemotactic factors, antimicrobial peptides and pro-inflammatory pathways in the yolk sac of broiler chicken embryos by Mila M. Y. Meijer, Henry van den Brand, Shahram Niknafs, Eugeni Roura

“…However, it is unknown whether carvacrol in the yolk could enhance defence responsiveness in the yolk sac. Therefore, the aim of this study was to improve early immune function in chicken embryos, and it was hypothesized that in ovo delivery of carvacrol would result in immunomodulatory effects in the yolk sac, potentially improving post-hatch resilience. …”
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Lactylation associated biomarkers and immune infiltration in aortic dissection by Jianfeng Ye, Yuntao Fu, Yuanjia Ke, Huanting Liu, Sini Fang, Yumeng Lei, Ying Mao, Liqiu Yan, Youcheng Wang

“…Abstract Protein lactylation, a novel post-translational modification (PTM), has emerged as a critical factor in disease processes related to glycolysis and immune responses. …”
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Identification of potential pathogenic genes associated with the comorbidity of rheumatoid arthritis and renal fibrosis using bioinformatics and machine learning by Jiao Qiu, Yalin Xu, Luyuan Tong, Xingchun Yang, Xiao Wu

“…Abstract This study aimed to identify the potential pathogenic genes associated with the comorbidity of rheumatoid arthritis (RA) and renal fibrosis (RF). Transcriptomic data related to RA and RF were retrieved from the GEO database. …”
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Evaluation of exploratory fluid biomarkers from a phase 1 senolytic trial in mild Alzheimer’s disease by Valentina R. Garbarino, Juan Pablo Palavicini, Justin Melendez, Nicolas R. Barthelemy, Yingxin He, Tiffany F. Kautz, Marisa Lopez-Cruzan, Julia J. Mathews, Peng Xu, Bin Zhang, Afaf Saliba, Nagarjunachary Ragi, Kumar Sharma, Dallin Mason, Samuel Johnson, Suzanne Hendrix, Suzanne Craft, Ronald C. Petersen, Jair Machado Espindola-Netto, Ailing Xue, Tamara Tchkonia, James L. Kirkland, Arash Salardini, Nicolas Musi, Randall J. Bateman, Mitzi M. Gonzales, Miranda E. Orr

“…Immunoassays, mass spectrometry and transcriptomics were performed and changes in analyte levels were assessed from baseline to post-treatment using paired t-tests. …”
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Single-nucleus and spatial signatures of the brainstem in normal brain and mild traumatic brain injury in male mice by Yuan Zhuang, Xixian Liao, Fei Niu, Ming Li, Yu Yan, Chuanhang He, Xiang Wu, Runfa Tian, Guoyi Gao

“…The spatial cellular connections within the brainstem or the mechanisms underlying its response to injury have been underestimated. Here, we performed single-nucleus RNA sequencing with spatial transcriptome sequencing in both normal and mTBI brainstems in male mice, revealing thirty-five neuron and non-neuron clusters. …”
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Cell type differences in human cytomegalovirus transcription and epigenetic regulation with insights into major immediate-early enhancer-promoter control. by Qiaolin Hu, Ming Li, Mrutyunjaya Parida, Benjamin M Spector, Juan F Santana, Arya Zandvakili, David H Price, Jeffery L Meier

“…Cell type differences in the human cytomegalovirus (HCMV) transcriptome may arise from variations in transcription or post-transcription regulation. …”
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Distinct patterns of innate immune activation by clinical isolates of respiratory syncytial virus. by Ruth Levitz, Yajing Gao, Igor Dozmorov, Ran Song, Edward K Wakeland, Jeffrey S Kahn

“…Viral transcriptome data indicated that there was a gradient of transcription across the RSV genome though in some strains, RSV G was the expressed in the highest amounts at late times post-infection. …”
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Characterization and optimization of mnn11Δ-mediated enhancement in heterologous protein production in Kluyveromyces marxianus by Shihao Zhou, Pingping Wu, Haiyan Ren, Jungang Zhou, Yao Yu, Hong Lu

“…Abstract Background N-glycosylation is a prevalent post-translational modification in eukaryotes, essential for regulating protein secretion. …”
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Lactylation Modification as a Promoter of Bladder Cancer: Insights from Multi-Omics Analysis by Yipeng He, Lingyan Xiang, Jingping Yuan, Honglin Yan

“…Lactylation modification is a newly discovered post-transcriptional modification of proteins, which is closely related to the occurrence and development of tumors. …”
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Temporal changes in the protein cargo of extracellular vesicles and resultant immune reprogramming after severe burn injury in humans and mice by Micah L. Willis, Micah L. Willis, Roland F. Seim, Laura E. Herring, Angie L. Mordant, Thomas S. Webb, Gilbert R. Upchurch, Ashish K. Sharma, Bruce A. Cairns, Philip A. Efron, Philip A. Efron, Shannon M. Wallet, Leon G. Coleman, Leon G. Coleman, Robert Maile, Robert Maile

“…Similarly, in human EVs at 14 days post-burn, increased levels of circulation/complement, immune, and transport proteins were detected.ConclusionsEVs from burn-injury patients at distinct time points differentially modulate immune responses in macrophages, mirroring the temporal immune phenotypes observed in clinical settings. …”
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Ischemic Preconditioning Confers Epigenetic Repression of Mtor and Induction of Autophagy Through G9a‐Dependent H3K9 Dimethylation by Olof Gidlöf, Andrea L. Johnstone, Kerstin Bader, Bohdan B. Khomtchouk, Jiaqi J. O'Reilly, Selvi Celik, Derek J. Van Booven, Claes Wahlestedt, Bernhard Metzler, David Erlinge

“…Background Ischemic preconditioning (IPC) protects the heart from prolonged ischemic insult and reperfusion injury through a poorly understood mechanism. Post‐translational modifications of histone residues can confer rapid and drastic switches in gene expression in response to various stimuli, including ischemia. …”
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Dose-dependent effects of gamma radiation on the early zebrafish development and gene expression. by Selma Hurem, Leonardo Martín Martín, Dag Anders Brede, Eystein Skjerve, Rasoul Nourizadeh-Lillabadi, Ole Christian Lind, Terje Christensen, Vidar Berg, Hans-Christian Teien, Brit Salbu, Deborah Helen Oughton, Peter Aleström, Jan Ludvig Lyche

“…The deformity frequency was significantly increased by prolonged exposure duration at dose rates ≥ 0.4 mGy/h. Molecular responses analyzed by RNA-seq at gastrulation (5.5 hpf transcriptome) indicate that the radiation induced adverse effects occurred during the earliest stages of development. …”
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Heightened Innate Immune and Inflammatory Gene Expression in the Skin of Severe COVID-19 Cases by Fernandes IG, Ferreira FM, De Sousa ESA, Pietrobon AJ, Teixeira FME, Ramos YÁL, Yendo TM, Pereira NZ, Sotto MN, Duarte-Neto AN, Orfali RL, Aoki V, Da Silva LFF, Duarte AJDS, Sato MN

“…In this study, we investigated the transcriptomic profile of skin samples from patients who succumbed to COVID-19.Patients and Methods: Skin autopsies from COVID-19 patients with post-mortem time of less than 20 hours were obtained from University of São Paulo Medical School Hospital and healthy skin samples, were submitted to RNA sequencing analysis. …”
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