Medical Hemorrhoid Gel Ameliorates Croton Oil-Induced Hemorrhoids by Suppressing the NLRP3 Inflammasome Activation via NF-κB Signaling Pathway by Ai Z, Yuan D, Li Y, Cai J, Zhou D, Liu W

“…Acute toxicity tests showed that MHG did not cause anorectal abnormalities or systemic toxicity in rats. Transcriptomic analysis integrated with experimental validation suggested the therapeutic mechanism of MHG involves inflammation response and NF-κB pathway. …”
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Circulating Tumor Cell Detection for Therapeutic and Prognostic Roles in Breast Cancer by Saiying Ma, Xiaojia Wang, Peter Ping Lin, Lei Lei

“…Methods This review systematically evaluates CTC detection methodologies, including immunoaffinity‐based approaches and biophysical techniques, which exhibit inherent trade‐offs in sensitivity, specificity, and compatibility with downstream analyses. Furthermore, post‐isolation molecular characterization methods spanning genomic, transcriptomic, and proteomic analyses are also critically assessed. …”
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Intrinsic Muscle Stem Cell Dysfunction Contributes to Impaired Regeneration in the mdx Mouse by Marie E. Esper, Caroline E. Brun, Alexander Y. T. Lin, Peter Feige, Marie J. Catenacci, Marie‐Claude Sincennes, Morten Ritso, Michael A. Rudnicki

“…We also studied MuSC dynamics and function under homeostatic conditions, during regeneration post‐acute injury, and following engraftment using a combination of histological and transcriptomic analyses. …”
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In silico identification of switching nodes in metabolic networks by Mairet, Francis

“…Finally, a comparison between ISIS and reporter metabolites identified with transcriptomic data confirms the key role of metabolites such as L-glutamate or L-aspartate in the yeast response to nitrogen input variation. …”
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Unravelling neutropenic enterocolitis: insights from gut microbiota, and intestinal barrier analyses by Natacha Kapandji, Maud Salmona, Anaïs Lemoine, Guillaume Ulmann, Julien Calderaro, Brigitte Roche, Nathalie Kapel, Lucie Biard, Etienne Lengline, Jérôme Le Goff, Christophe Rodriguez, Muriel Thomas, Lara Zafrani

“…Severe NE is marked by the development of a local inflammatory response that may be induced by the reduction in butyrate levels. …”
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High-rate mechano-stimulation alters proliferation- and maturation-related signaling of oligodendrocyte precursor cells in a 3D hydrogel by Ryosuke Yokosawa, Rachel A. Mazur, Kelsey A. Wilson, Jacob H. Lee, Noah W. Showalter, Kyle J. Lampe, Pamela J. VandeVord

“…Thus, this study aims to elucidate the cellular responses of OPCs using a brain-tissue mimicking in vitro 3D hydrogel platform to identify key signaling pathways driving their response. …”
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Multi-omics-based construction of a palmitoylation-driven prognostic model reveals tumor immune phenotypes in osteosarcoma by Jingyu Chen, Feihu Chen, Haiping Zhang, Yuanlong Hu, Xingyu Zhou, Xijia Weng, Guofeng Bao, Xiaomin Ding

“…Abstract Background Osteosarcoma (OS), the leading primary malignancy of bone in adolescents, is known for its aggressive metastatic behavior and poor responsiveness to conventional therapies. As a lipid-mediated post-translational modification, protein palmitoylation has gained attention for its pivotal role in modulating oncogenic signaling pathways and facilitating tumor immune escape. …”
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Cardiac Sympathetic Denervation Mitigated Ischemic Cardiomyopathy Progression in a Rat Model of Sleep Apnea by Maximin Détrait, Jonathan Gaucher, Emma Billoir, Sophie Bouyon, Emeline Lemarié, Stéphanie Paradis, Guillaume Vial, Florent Chuffart, Côme Pasqualin, Fabien Hubert, Francesca Rochais, Stéphane Doutreleau, Jean‐Louis Pépin, Diane Godin‐Ribuot, Elise Belaidi, Claire Arnaud

“…At 14 weeks, sympathovagal balance, cardiac function, and remodeling were evaluated. Results Post myocardial infarction, CSD prevented IH‐induced blunting of cardiomyocyte response to isoproterenol challenge and reduced cardiac sympathetic activity. …”
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Unlocking prognostic potential: A genomic signature of caloric restriction in patients with epithelial ovarian cancer. by Ori Tal, Tamar Zahavi, Liat Anabel Sinberger, Mali Salmon-Divon

“…<h4>Objectives</h4>Epithelial ovarian cancer is a significant contributor to cancer-related mortality in women, frequently recurring post-treatment, often accompanied by chemotherapy resistance. …”
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Innate biosignature of treatment failure in human cutaneous leishmaniasis by María Adelaida Gómez, Ashton Trey Belew, Deninson Alejandro Vargas, Lina Giraldo-Parra, Neal Alexander, David E. Rebellón-Sánchez, Theresa A. Alexander, Najib M. El-Sayed

“…Through sequential transcriptomic profiling of blood monocytes, neutrophils, and eosinophils over the course of systemic treatment with meglumine antimoniate, we revealed that a heightened and sustained Type-I interferon response signature is a hallmark of treatment failure (TF) in CL patients infected with Leishmania (Viannia) panamensis and L.V. braziliensis. …”
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Single-cell RNA sequencing reveals the heterogeneity and regulatory modules of cell-specific RNA-binding proteins in patients with systemic lupus erythematosus by Sheng-Yan Lin, Yikai Yu, Daan Nie, Lili Yang, Yuxue Chen, Yu Chen, Cheng Wen, Zhipeng Zeng

“…RNA-binding proteins (RBPs), which orchestrate post-transcriptional gene regulation through dynamic interactions with target transcripts, are increasingly implicated in autoimmune pathogenesis. …”
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Single-Cell Transcriptional Analysis Reveals the Mechanism of AZD6738 in HCC Immunotherapy via EZH2 Targeting by Li RP, Wu GZ, Fang XD, Yang WW, Zhang HY, Yue HX, Zheng Y, Wang YP, Zhou YN

“…Additionally, we explore the specific effects of AZD6738 on the tumor microenvironment and its ability to regulate immune responses.Methods: We employed a combination of network pharmacology and transcriptomic analysis to identify specific molecules associated with HCC, including EZH2, CCNB1, PRKDC, CTSL, PSEN1, SLC6A3, and FKBP1A. …”
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Dysfunctional immunity in the comorbidity of vitamin A deficiency and tuberculosis identifies therapeutic potential of vitamin A supplementation by Dr. Faye Lanni, Revanth Krishna, David Forrest Ackart, Victoria Mitcham, Sarah K Cooper, Connor King, Hadley Gary, Dr. Chuangqi Wang, Dr Brendan Podell

“…Guinea pigs with dietary-induced pre-existing vitamin A deficiency were exposed to a low dose aerosol of Mycobacterium tuberculosis (Mtb) and compared to Mtb exposed guinea pigs with sufficient dietary vitamin A. Immune response was evaluated at multiple endpoints post-infection by histopathology and flow cytometry, incorporating single cell RNAseq and spatial transcriptomics and in a subset of the guinea pigs. …”
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Lactobacilli-based postbiotic differentially affects chicken macrophage-like HD11 cells depending on stimulatory lipopolysaccharide dosage by Samuel C. G. Jansseune, Jürgen van Baal, Fany Blanc, Aart Lammers

“…Results Post increased NO production dose-dependently and an LPS-postbiotic interaction was present, with the cells eliciting a higher NO production in response to Post at 30 and 300 ng/ml LPS compared to the zero and 3 ng/ml LPS. …”
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Site-2 protease Sll0528 interacts with RbcR to regulate carbon/nitrogen homeostasis in the cyanobacterium Synechocystis sp. PCC 6803 by Shiqi Lin, Taotao Zheng, Yongyi Mo, Ge Zhang, Gu Chen

“…TurboID-based proximity labeling, coupled with quantitative proteomics, revealed a robust set of putative Sll0528-interacting proteins, some of which were subsequently validated through bacterial two-hybrid assays and transcriptomic profiling. Notably, we confirmed the physical interaction between Sll0528 and RbcR, a low-carbon-responsive transcriptional regulator. …”
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A multivariate cell-based liquid biopsy for lung nodule risk stratification: Analytical validation and early clinical evaluation by Jason D. Berndt, Fergal J. Duffy, Mark D. D'Ascenzo, Leslie R. Miller, Yijun Qi, G. Adam Whitney, Samuel A. Danziger, Anil Vachani, Pierre P. Massion, Stephen A. Deppen, Robert J. Lipshutz, John D. Aitchison, Jennifer J. Smith

“…In the platform, standardized cells are exposed to small volumes of patient serum, and the resulting transcriptomic response is analyzed using machine learning tools to develop disease classifiers. …”
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Integration of single‐cell and bulk RNA‐sequencing data reveals the prognostic potential of epithelial gene markers for prostate cancer by Zhuofan Mou, Lorna W. Harries

“…Current clinicopathological factors inadequately predict biochemical recurrence, a critical indicator guiding post‐treatment strategies following radical prostatectomy. …”
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Neuropathology-based approach reveals novel Alzheimer's Disease genes and highlights female-specific pathways and causal links to disrupted lipid metabolism: insights into a viciou... by Yin Jin, Apostolia Topaloudi, Sudhanshu Shekhar, Guangxin Chen, Alicia Nicole Scott, Bryce David Colon, Petros Drineas, Chris Rochet, Peristera Paschou

“…Our sex-specific analysis points to a role for BIN1 specifically in women as well as novel AD loci including QRFPR and SGCZ. Post-GWAS analyses illuminate the functional and biological mechanisms underlying AD and reveal sex-specific differences. …”
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Single-cell RNAseq and longitudinal proteomic analysis of a novel semi-spontaneous urothelial cancer model reveals tumor cell heterogeneity and pretumoral urine protein alterations... by Iliana K Kerzeli, Martin Lord, Milena Doroszko, Ramy Elgendy, Aikaterini Chourlia, Ivan Stepanek, Elinor Larsson, Luuk van Hooren, Sven Nelander, Per-Uno Malmstrom, Anca Dragomir, Ulrika Segersten, Sara M Mangsbo

“…The profiling of the model was performed by histology grading, single cell transcriptomic and proteomic analysis, while the derivation of a tumorigenic cell line was validated and used to assess in vivo anti-tumor effects in response to immunotherapy. …”
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Pathological and Molecular Characterization of Grass Carp Co-Infected with Two <i>Aeromonas</i> Species by Wenyao Lv, Zhijie Zhou, Lingli Xie, Xinyue Wang, Yifei Zhou, Lang Gui, Xiaoyan Xu, Yubang Shen, Jiale Li, Junqiang Qiu

“…Mortality was observed as early as 24 h post-infection, with cumulative mortality reaching 68%. …”
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