Reversible ubiquitination conferred by domain shuffling controls paired NLR immune receptor complex homeostasis in plant immunity by Zhiyi Chen, Jianhua Huang, Jianyu Li, Frank L. H. Menke, Jonathan D. G. Jones, Hailong Guo

“…Our findings improve our understanding of homeostatic regulation of paired NLR complex and uncover a paradigm whereby domain integration can co-opt preexisting post-translational modification to regulate novel protein functions.…”
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The novel mouse mutation Oblivion inactivates the PMCA2 pump and causes progressive hearing loss. by Sarah L Spiden, Mario Bortolozzi, Francesca Di Leva, Martin Hrabé de Angelis, Helmut Fuchs, Dmitry Lim, Saida Ortolano, Neil J Ingham, Marisa Brini, Ernesto Carafoli, Fabio Mammano, Karen P Steel

“…Obl/+ mutants showed increasing hearing impairment from post-natal day (P)20 to P90, and loss of auditory function was followed by a corresponding base to apex progression of hair cell degeneration. …”
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Identification of fatty acid metabolism-related genes in the tumor microenvironment of breast cancer by a development and validation of prognostic index signature by Zhaofeng Ma, Man Zheng, Pulin Liu

“…We aimed to show how FAMGs influence immune function, immune checkpoints, and m6a in BRCA. A co-expression analysis discovered that gene expression is strongly connected to pyroptosis. …”
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Overcoming the detrimental impact of volumetric muscle loss on segmental fracture healing via the induced membrane technique by Andrew R. Clark, Michael S. Valerio, Jonathan Kulwatno, Sergey S. Kanovka, Andrew L. Ferrer, Christopher L. Dearth, Stephen M. Goldman

“…At the 12-week postoperative mark, a comprehensive analysis was conducted, including endpoint µCT analysis, evaluation of neuromuscular function, tibia torsional testing, and histological examination. …”
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolbox by Megan D. Schertzer, Andrew Stirn, Keren Isaev, Laura Pereira, Stella H. Park, Anjali Das, Aline Réal, Erin D. Jeffery, Claire Harbison, Hans-Hermann Wessels, Gloria M. Sheynkman, Neville E. Sanjana, David A. Knowles

“…Two major challenges in characterizing the cellular function of alternative isoforms are the lack of experimental methods to specifically and efficiently modulate isoform expression and computational tools for complex experimental design and analysis. …”
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Knowledge and attitude of dental undergraduates, graduates, and postgraduates on dental implants for children – A cross-sectional study by Ramya Ramanathan, Paul Chalakkal, Ridhima Gaunkar, Vidya Baliga

“…Dental implants in children restore function, preserve the alveolar bone, and give excellent esthetics, restoring the child’s confidence and social acceptability. …”
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Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways. by Ludovic Vallier, Thomas Touboul, Zhenzhi Chng, Minodora Brimpari, Nicholas Hannan, Enrique Millan, Lucy E Smithers, Matthew Trotter, Peter Rugg-Gunn, Anne Weber, Roger A Pedersen

“…Here, we analysed the function of key developmental growth factors such as Activin, FGF and BMP in the control of early cell fate decisions of human pluripotent stem cells. …”
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Can muscle synergies shed light on the mechanisms underlying motor gains in response to robot-assisted gait training in children with cerebral palsy? by Gloria P. Vergara-Diaz, Stefano Sapienza, Jean-François Daneault, Eric Fabara, Catherine Adans-Dester, Giacomo Severini, Vincent C. K. Cheung, Carmen Echevarría Ruiz de Vargas, Donna Nimec, Paolo Bonato

“…Children participated in a 6 week-RGT intervention and underwent clinical evaluations and gait studies—with focus on the analysis of electromyographic (EMG) data—pre- and post-training. …”
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Correlation between Blood and CSF Compartment Cytokines and Chemokines in Subjects with Cryptococcal Meningitis by Elizabeth C. Okafor, Katherine H. Hullsiek, Darlisha A. Williams, James E. Scriven, Joshua Rhein, Henry W. Nabeta, Abdu K. Musubire, Radha Rajasingham, Conrad Muzoora, Charlotte Schutz, Graeme Meintjes, David B. Meya, David R. Boulware

“…There was no correlation at 1-week post cryptococcal diagnosis. However, at 2-week post cryptococcal diagnosis, there was a weak positive correlation of granulocyte-macrophage colony-stimulating factor levels (Rho=0.25; p=.007) in serum and cerebrospinal fluid. …”
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Health technology assessment (HTA) of prostatic urethral lift (PUL) for the treatment of benign prostatic hyperplasia (BPH) in the Italian context by Agostino Fortunato, Eugenio Di Brino, Michele Basile, Debora Antonini, Filippo Rumi

“…A Budget Impact Model (BIM) simulated treatment pathways over five years, comparing UroLift with TURP and HoLEP. Results: As the analysis shows, despite the higher initial acquisition cost, UroLift generates savings for the NHS in all the years considered within the analysis. …”
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Dynamic Monitoring of Goaf Stress Field and Rock Deformation Driven by Optical Diber Sensing Technology by Jing Chai, Zhe Yan, Yibo Ouyang, Dingding Zhang, Jianfeng Yang, Gaoyi Yang, Chenyang Ma

“…By coupling the fracture mechanics model of the critical layer and the settlement trajectory function model, the dynamic transformation of the pre-fracture and post-fracture stages is realized, and the stress evolution can be monitored and predicted in real time. …”
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Phosphorylation of Plant Ferredoxin-like Protein Is Required for Intensifying PAMP-Triggered Immunity in <i>Arabidopsis thaliana</i> by Tzu-Yi Chen, Rui-Wen Gong, Bo-Wei Chen, Yi-Hsien Lin

“…These findings suggest that phosphorylation at the T90 residue might be essential for PFLP-mediated enhancement of plant immune responses, implying that this post-translational modification is likely required for its disease resistance function in planta. …”
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Association of time in range with cognitive impairment in middle-aged type 2 diabetic patients by Yanting Liu, Yanlan Liu, Huina Qiu, Nahal Haghbin, Jingbo Li, Yaoshuang Li, Weiran Jiang, Longfei Xia, Fan Wu, Chenying Lin, Jingna Lin, Chunjun Li

“…Abstract Objective This study investigated the association of Time In Range (TIR) obtained from Blood Glucose Monitoring (BGM) with Cognitive Impairment (CI) inpatients with middle-aged Type 2 Diabetes Mellitus (T2DM) and further explored whether a TIR goal for T2DM in adults with > 70% possess a protective effect on cognitive function. Research design and methods A total of 274 inpatients with T2DM aged 40–64 years, who underwent seven-point BGM ( pre meals and 120 min post meals and at bedtime) were recruited in this cross-sectional study. …”
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Patient-centered outcomes on preparing for and undergoing gender-affirming phalloplasty: a qualitative, descriptive study by Daphna Harel, Asa Radix, Rachel Bluebond-Langner, Tenny R Zhang, Carolyn A Berry, Nnenaya Mmonu, Elijah Castle, Lee Zhao, Clarissa Ospina-Norvell, Mark Fendrick

“…The objective of this study is to elucidate transgender and non-binary patient perspectives on gender-affirming phalloplasty/metoidioplasty via structured focus groups and determine convergent themes as the first step towards the development of a GGAS patient-reported outcome measure.Design We conducted a systematic qualitative study using a thematic content analysis of four focus groups from April 2021 to April 2022 comprising 8 patients undergoing phalloplasty/metoidioplasty and 10 patients post-phalloplasty/metoidioplasty. …”
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Clinical and Histopathological Determinants for Kidney Allograft Survival in the Eurotransplant Senior Program (ESP) at the Time of Allocation by Tom N. Langer, Tom N. Langer, Thorsten Wiech, Thorsten Wiech, Mercedes Noriega, Sergey Biniaminov, Tobias B. Huber, Tobias B. Huber, Lutz Fischer, Lutz Fischer, Florian Grahammer, Florian Grahammer, Florian Grahammer, Malte A. Kluger, Malte A. Kluger

“…This study aimed to evaluate post-transplant outcomes of deceased donor grafts and identify prognostic factors within the ESP population. …”
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The ASSIST Trial: Acute Effects of Manipulating Strength Exercise Volume on Insulin Sensitivity in Adults Living With Obesity: A Randomized Controlled, Crossover, Clinical Trial by Luis Filipe Rocha Silva, Paulo H. Evangelista Silva, Jordan Ottoni do Carmo, Bruna Caroline Chaves Garcia, Elizabethe Adriana Esteves, Zachary Aaron Mang, Fabiano Trigueiro Amorim, Marco Fabrício Dias‐Peixoto, Fernando Gripp, Valmor Tricoli, Flavio de Castro Magalhaes

“…Results One‐way repeated measure analysis of variance followed by Tukey as post hoc showed that indexes of insulin sensitivity improved after the high‐volume session (p < 0.05) but not after the low‐volume session (p > 0.05) (effect size [EF] for high and low volume, respectively): insulin area under the curve (AUC) [−0.53 and −0.05], C‐peptide AUC [0.58 and 0.18], Cederholm index [0.59 and 0.11], skeletal muscle index [0.89 and 0.60], Gutt index [0.78 and 0.20], Stumvoll index [0.81 and 0.18], and the simple index [0.52 and 0.16]). β‐cell function showed a strong tendency to improve (p < 0.06) after the high‐volume session (EF for high and low volume, respectively): C‐peptidogenic index [0.58 and −0.20], C‐peptide oral disposition index [0.72, −0.33]. …”
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Characteristics of myocardial work during exercise stress echocardiography in healthy adults by Liwei Huang, Liwei Huang, Luwei Ye, Luwei Ye, Hongmei Zhang, Hongmei Zhang, Qingfeng Zhang, Qingfeng Zhang, Geqi Ding, Geqi Ding, Chunmei Li, Chunmei Li, Yan Deng, Yan Deng, Lixue Yin, Lixue Yin, Yi Wang, Yi Wang

“…Furthermore, the multivariable linear regression analysis showed that GWE was independently associated with PPS (β = −0.395, p = 0.018) and EF (β = −0.236, p &lt; 0.001).ConclusionSex had a minimal effect on MW-based LV systolic function in healthy adults. …”
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Proteomics profiling of inflammatory responses to elexacaftor/tezacaftor/ivacaftor in cystic fibrosis by Hazel Ozuna, Hazel Ozuna, Dinesh Bojja, Santiago Partida-Sanchez, Luanne Hall-Stoodley, Amal Amer, Rodney D. Britt, Rodney D. Britt, Shahid Sheikh, David A. Frank, Weiyuan Wang, Bum-Yong Kang, Bum-Yong Kang, Irina Miralda, Irina Miralda, Samantha L. Durfey, Samantha L. Durfey, Benjamin T. Kopp, Benjamin T. Kopp

“…Paired analysis (CF pre- and CF post-ETI) of differential protein expression demonstrated significant expression changes of MMP-10, EN-RAGE, and IL-17A. …”
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Dysregulation of Retinal Melatonin Biosynthetic Pathway and Differential Expression of Retina-Specific Genes Following Blast-Induced Ocular Injury in Ferrets by Chetan Pundkar, Rex Jeya Rajkumar Samdavid Thanapaul, Manoj Govindarajulu, Gaurav Phuyal, Joseph B. Long, Peethambaran Arun

“…Methods: In this study, we utilized a ferret model of bTOI to evaluate the temporal expression of melatonin-synthesizing enzymes, such as tryptophan hydroxylase 1 and 2 (<i>TPH</i>1 and <i>TPH</i>2), Aralkylamine N-acetyltransferase (<i>AANAT</i>), and Acetylserotonin-O-methyltransferase (<i>ASMT</i>), and retina-specific genes (<i>Rhodopsin</i>, <i>Melanopsin</i>) and retinal pigment epithelium-specific 65 kDa protein (<i>RPE65</i>) at 4 h, 24 h, 7 days, and 28 days post-blast. Ferrets were exposed to tightly coupled blast overpressure waves using an advanced blast simulator, and retinal tissues were collected for quantitative polymerase chain reaction (qPCR) analysis. …”
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Surgery/anesthesia may cause monocytes to promote tumor development by Yang-Yang Wang, Rui-Lou Zhu, En-Qiang Chang, Xiao-Zhuan Liu, Guang-Zhi Wang, Ning-Tao Li, Wei Zhang, Jun Zhou, Ming-Yang Sun, Xin Zou, Jie Hao, Jia-Qiang Zhang

“…Results The ratio of CD4+ and CD8+ T cells and Tregs showed little change, and the function of CD4+ and CD8+ T cells recovered soon. The function of Treg had not been restored 48 h post operation. …”
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