Preliminary Results of Reduced Polymerase Chain Reaction (PCR) Volumes When Analysing Low Template DNA Samples with Globalfiler™ and Yfiler™ Plus Kits by Jesús Martínez-Gómez, Sheila Laso-Izquierdo, Araceli Vera-Yánez, José Juan Fernández-Serrano, Cláudia Gomes

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Diagnostic Challenges of Tuberculous Lymphadenitis Using Polymerase Chain Reaction Analysis: A Case Study by Hirokazu Taniguchi, Masahiko Nakamura, Kazuki Shimokawa, Fumi Kamiseki, Shin Ishizawa, Hitoshi Abo, Hideaki Furuse, Takeshi Tsuda, Yasuaki Masaki, Kensuke Suzuki

“…She was treated with antitubercular drugs and subsequently had a reduction in cervical lymph node swelling. Polymerase chain reaction analysis is not 100% accurate; hence, its use as a diagnostic tool for mycobacterial infection requires increased attention.…”
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Polymerase Chain Reaction in the Management of Patients with Anterior Ophthalmoherpes: a Comparative Study of the Effectiveness of Clinical Use by G. M. Chernakova

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Evaluation of a test-and-cull strategy to control bovine paratuberculosis based on the detection of Mycobacterium avium ssp. paratuberculosis DNA using the high-throughput Johne's... by A.A. Garcia, K.M. Plain, J.-A.L.M.L. Toribio, R.J. Whittington

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Subgingival air-polishing treatment in patients with aggressive periodontitis by Trtić Nataša, Bošnjak Andrija P., Arbutina Radmila, Nikolić Tatjana, Marin Saša, Veselinović Valentina, Kojić Željka, Adamović Tijana, Dolić Olivera

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An in vivo comparative evaluation of antimicrobial efficacy of chitosan, chlorhexidine gluconate gel and their combination as an intracanal medicament against Enterococcus faecalis... by A. Savitha, A. SriRekha, R. Vijay, Ashwija, C. Champa, T. Jaykumar

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Periorbital cellulitis with Zoster encephalitis: a case report by Mustafa Usanmaz, Bahar Busra Sivrikaya, Ozgur Gunal, Mehmet Hakan Taskin, Duygu Yener

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Clinical and microbiological effects of photodynamic therapy applied in non-surgical treatment of periodontitis by Arsić Zoran, Jovanović Radovan, Đorđević Aleksandar, Šehalić Meliha, Marjanović Dragan, Mikić Mirko, Vlahović Zoran, Mladenović Raša

“…The presence of microorganisms Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Treponema denticola was detected by the polymerase chain reaction (PCR) method. Samples were tested before the therapy, as well as three months after the therapy. …”
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Evaluating of HERV-K Genes Expression in Sperm Samples Collected from Infertile Men Compared to Fertile Individuals: A Case-Control Study by Shaian Tavakolian, Zahra Rafiei Atani, Amir Zarei, Hossein Goudarzi, Amir Reza Abedi, Ebrahim Faghihloo

“…After extracting the RNA form these samples, we evaluated the expression of HERV-K env, np9, rec, and gag using quantitative real-time polymerase chain reaction (qRT-PCR). Results: Our data revealed that HERV-K, rec, np9, and env in abnormal samples were higher than normal ones. …”
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Design and comparison of PCR-ELISA reaction with other available hybridization methods to identify types 11, 16, and 18 of the human papillomavirus by Seyed Mohammad Amin Mousavi-Rad, Shohreh Zare Karizi, Hamid Sedighian, Seyed Ali Mirhosseini, Hadi Esmaeili Gouvarchin ghaleh, Jafar Amani

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The effect of a phytogenic-based feed additive on concurrent Lawsonia intracellularis and Brachyspira hyodysenteriae infections in pigs by CF Wu, HC Kuo, M Glišić, M Vasiljević, J Raj, J Bošnjak- Neumuller, V Drašković

“…The bacterial contents of the samples were determined using real-time polymerase chain reaction, and at the end of the experiment, histological changes in ileal samples were examined. …”
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Evaluating Hemagglutination Inhibition Antibody Titers as a Correlate of Protection for Influenza: A Sensitivity Analysis Based on Information Theory and Causal Inference by Fenny Ong, Geert Molenberghs, Andrea Callegaro, Wim Van Der Elst, Geert Verbeke, Florian Stijven, Ingrid Van Keilegom, Ariel Alonso Abad

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The Impact of Allicin on the Growth of <i>Clostridium</i> spp. in the Digestive Track of Quails by Aleksandra Makuch, Monika Ziomek, Magdalena Sapała, Kamil Drabik, Justyna Batkowska, Piotr Domaradzki, Ewelina Patyra, Tomasz Grenda

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Results of investigating the characteristics of bone marrow in patients with squamous cell carcinoma of the head and neck, their clinical value by E. G. Timonina, N. N. Tupitsyn, S. O. Podvyaznikov, V. A. Spiridonova, M. A. Frenkel’, O. P. Kolbatskaya, O. A. Chegrinets

“…A highly sensitive method for detecting mRNA of the specific squamous cell carcinoma protein E48 was perfected using reverse-transcription polymerase chain reaction and Southern blotting, followed by an enzyme immunoassay that could recognize bone marrow micrometastases in 35 % of the patients with HNSCC. …”
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Case Report of Chronic Myeloid Leukemia with Coexistent Sickle Cell Anemia Heterozygous Presentation by Bala Manideep Nuguri, Samira Kumar Behera, Prasanta Purohit

“…Clinical examination, complete blood counts, peripheral smear, sodium metabisulphate oxygen reduction, hemoglobin electrophoresis, HPLC [variant II beta thalassemia short program], and molecular study using reverse transcriptase enzyme followed by conventional polymerase chain reaction (PCR). …”
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Gut microbiota and graft-versus-host disease in hematopoietic stem cell transplant patients by Pegah Panahi, Amir Hossein Hashemian, Mehrdad Payandeh, Mahdi Taghadosi, Bizhan Nomanpour

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The combination of poly(ADP-ribose) polymerase inhibitor and statin inhibits the proliferation of human castration-resistant and taxane-resistant prostate cancer cells in vitro and... by Yoshitaka Sekine, Daisuke Oka, Akira Ohtsu, Hiroshi Nakayama, Takeshi Miyao, Yoshiyuki Miyazawa, Seiji Arai, Hidekazu Koike, Hiroshi Matsui, Yasuhiro Shibata, Kazuhiro Suzuki

“…Methods PC-3, LNCaP, and 22Rv1 human prostate cancer cell lines were used to develop androgen-independent LNCaP cells (LNCaP-LA). mRNA and protein expression levels were evaluated by quantitative real-time polymerase chain reaction and western blot analysis, respectively. …”
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Sex discrepancy in the reduction of mucosal‐associated invariant T cells caused by obesity by Jianyun Liu, Hongmei Nan, Randy R Brutkiewicz, Jose Casasnovas, Kok Lim Kua

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Polysaccharides from Marine Bacteria and Their Anti-SARS-CoV-2 Activity by Tatyana A. Kuznetsova, Natalia V. Krylova, Maksim S. Kokoulin, Elena V. Persiyanova, Olga S. Maistrovskaya, Pavel. G. Milovankin, Yurii A. Belov, Mikhail Yu. Shchelkanov

“…The activity of PSs against SARS-CoV-2 was assessed by inhibition of the virus cytopathogenic effect (<i>CI</i>) in the methylthiazolyl tetrazolium (MTT) test and using a real-time reverse transcription polymerase chain reaction (RT-PCR-RV). Results of the study demonstrate that PSs, which differ in chemical structure, exhibited anti-SARS-CoV-2 activity differences. …”
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Brazilian spotted fever in urban environments: control strategies and measures for risk reduction by Clóvis Gomes de Carvalho Júnior, Vinícius Silva Belo, Rafael Gonçalves Teixeira Neto, Frederico Rodrigues Ramalho, Tiago Oliveira Maciel, Marcos Vinícius Rodrigues, Ingrid Benevides Machado, David Soeiro Barbosa, Gilberto Salles Gazeta, Eduardo Sérgio da Silva

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