Two-step Red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli by B. Karstentischer, Jens von Einem, Benedikt Kaufer, Nikolaus Osterrieder

“…We applied the method to modification of bacterial artificial chromosome (BAC) constructs harboring an infectious herpesvirus clone to demonstrate the potential of the mutagenesis technique, which was used for the insertion of long sequences such as coding regions or promoters, introduction of point mutations, scarless deletions, and insertion of short sequences such as an epitope tag. …”
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The Policy-Making Model Based on Conflict of Interest Management in Crisis Conditions in the Country\'s Health System (Case of Study: The Experience of COVID-19) by Mahnaz sabzi, abbas Monavarian, Tayebeh Abbasi

“…Data analysis was done in three stages of open coding, axial coding, and selective coding using MaxQDA10 software. …”
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Dna-storalator: a computational simulator for DNA data storage by Gadi Chaykin, Omer Sabary, Nili Furman, Dvir Ben Shabat, Eitan Yaakobi

“…However, the unique nature of the errors in DNA, which include insertion, deletion, and substitution errors, requires the development of new algorithmic and coding solutions for these storage systems. Results The DNA-Storalator is a cross-platform software tool that simulates in a simplified digital point of view biological and computational processes involved in the process of storing data in DNA molecules. …”
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Whole genome characterization of the mechanisms of daptomycin resistance in clinical and laboratory derived isolates of Staphylococcus aureus. by Anton Y Peleg, Spiros Miyakis, Doyle V Ward, Ashlee M Earl, Aileen Rubio, David R Cameron, Satish Pillai, Robert C Moellering, George M Eliopoulos

“…EM and lipid membrane composition analyses on two clinical pairs showed that the daptomycin-nonsusceptible strains had a thicker cell wall and an increase in membrane lysyl-phosphatidylglycerol.<h4>Conclusion</h4>Point mutations in genes coding for membrane phospholipids are associated with the development of reduced susceptibility to daptomycin in S. aureus. …”
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ORF1ab codon frequency model predicts host-pathogen relationship in orthocoronavirinae by Phillip E. Davis, Joseph A. Russell

“…These models point to a small subset of features, specifically 5 codons, that are critical to the success of the models. …”
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Pseudorandom ultrasonic Doppler meter of liquid flow and profile with digital serial signal processing by Z. TRAWIŃSKI, D. J. CATHIGNOL

“…Previously a flowmeter with pseudorandom coding of the transmitted signal made it possible to measuring point on a precisely determined depth with respect to the position of the ultrasonic probe. …”
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Experiences and Perspective of Caretakers Working in Childcare Institutes in Delhi: A Qualitative Inquiry by Prerna Sharma, Yashika Sharma

“…However, the perspective of caregivers who are an important point of contact with the children is yet to be explored thoroughly. …”
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Online Islamic knowledge sources and their authority in Islamic learning: a case study of Indonesian Muslim universities by Askar Askar, Nurdin Nurdin, Adawiyah Pettalongi, Sagaf S. Pettalongi, Jumri H. I. Thahang Basire

“…The data analysis was iteratively begun with open coding, selective coding, and theoretical findings. …”
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Measurement Method for Plane Transverse Vibration of Hoisting Steel Wire Rope by Hough Transform by YAO Jiannan; XING Qiang; XU Haoran

“…According to the feature that the steel wire rope is mapped into the image with a form of line, first, image binaryzation, thinning, dilation and noise reduction are carried out on the initial static image, line extraction and coding are then conducted by Hough transform. Subsequently, a precision solving method to get the measured point on the line is developed based on the results of Hough transform; then, camera calibration is done with edge detection, on the basis of the region of interest, the dynamic displacement of the measured point is acquired by pixel traversing. …”
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Genotype-phenotype correlation in Brazillian Rett syndrome patients Correlação genótipo-fenótipo em pacientes brasileiras com síndrome de Rett by Fernanda T. de Lima, Decio Brunoni, José Salomão Schwartzman, Maria Cristina Pozzi, Fernando Kok, Yara Juliano, Lygia da Veiga Pereira

“…METHOD: Clinical evaluation of 105 patients, following a standard protocol. Detection of point mutations on the MECP2 gene was performed on peripheral blood DNA by sequencing the coding region of the gene. …”
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DiffInvex identifies evolutionary shifts in driver gene repertoires during tumorigenesis and chemotherapy by Ahmed Khalil, Fran Supek

“…We apply DiffInvex to >11,000 somatic whole-genome sequences from ~30 cancer types or healthy tissues, identifying genes where point mutations are under conditional positive or negative selection during exposure to specific chemotherapeutics, suggesting drug resistance mechanisms occurring via point mutation. …”
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Twenty-five years since the identification of the first SCA gene: history, clinical features and perspectives for SCA1 by Carlos Roberto Martins Junior, Fabrício Castro de Borba, Alberto Rolim Muro Martinez, Thiago Junqueira Ribeiro de Rezende, Iscia Lopes Cendes, José Luiz Pedroso, Orlando Graziani Povoas Barsottini, Marcondes Cavalcante França Júnior

“…The aim of this article is to review, from an historical point of view, the first CAG-related ataxia to be genetically described: SCA 1.…”
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Time Evolution of the Densities of Three Species Interacting in the Same Ecosystem and the Stability Analysis of Steady States for a Reaction–Diffusion PDE Model by Emmanuel Niyigaba, Wellars Banzi, Hamidou Touré

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Practice Report as a Form of Graduation Thesis among Master of Interpreting Students in China by Jiachen Wen, Xiangdong Li

“…A purposive sample of 685 interpreting practice reports (2015-2021) were analyzed through content analysis against a literature-informed coding scheme. The results reveal the diversified features of MI graduates’ interpreting practice and point to the lack of agreed norms on report writing. …”
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Cloning and expression analysis of OCPI2 gene in rice by XU Jingang1,2, REN Xinxin2, WANG Haibing2, WANG Baixue2, JING Shengli2, LIU Qingsong2,3*

“…To investigate the function of protease inhibitor genes in rice defense against herbivorous insects, the coding sequence of the protease gene OCPI2 from the rice variety ‘Zhonghua 11' was cloned. …”
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Presenting a Model for Color Effectiveness in Marketing Using Consumer Neuroscience: A Sequential Exploratory Mixed-Methods Approach by Allahyar Ghasemi, Ahmad Rahchamani, Hamidreza Saeednia

“…Also, changes in arousal and valence of research participants were tested using a facial movement coding system and Face Reader software. ConclusionFrom the point of marketing, Organizations by creating their organizational color, which is based on the correct application of cognitive and emotional responses of their consumers and then testing these responses more exactly by applying consumer neuroscience, can benefit from the advantage of utilization of precise neuroscience tools. …”
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Biosynthesis and Regulatory Mechanisms of Plant Flavonoids: A Review by Yuye Mao, Jiajia Luo, Zeping Cai

“…Consequently, the biosynthesis and regulatory mechanisms of flavonoids have been a focal point in plant transcription and gene expression research. …”
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The efficiency evaluation of taekwondo coaches by Njegovan Jovana

“…In the quantitative part, research instruments such as a questionnaire for coaches and a five-point Likert scale were used for the assessment and self-assessment of taekwondo coaches' professionalism, along with an interview protocol for coaches. …”
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Electrochemical Strategies for MicroRNA Quantification Leveraging Amplification and Nanomaterials: A Review by Alexander Hunt, Gymama Slaughter

“…MicroRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression and have emerged as critical biomarkers in various diseases, including cancer. …”
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Representaciones sociales sobre el perdón: la perspectiva de víctimas del conflicto armado by Jesús Ernesto Urbina-Cárdenas, Mildred Johana Miranda-Cordero, Magda Belén Bayona-Sanabria

“…The results point to emerging categories for understanding the phenomenon, such as: definitions of forgiveness, aspects or factors that facilitate forgiveness, aspects or factors that hinder forgiveness, and the positive effects of forgiveness.…”
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