An integrated approach to the characterization of immune repertoires using AIMS: An Automated Immune Molecule Separator. by Christopher T Boughter, Martin Meier-Schellersheim

“…Here, we introduce a software package for the integrated analysis of immune repertoire data, capable of identifying distinct biophysical differences in isolated TCR, MHC, peptide, antibody, and antigen sequence data. …”
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Computational design of multi-epitope vaccine against Hepatitis C Virus infection using immunoinformatics techniques. by Sara Zubair, Fahed Parvaiz, Turki Abualait, Khalid Al-Regaiey, Tasneem Anwar, Mahnoor Zafar, Imdad Kaleem, Shahid Bashir

“…In this study, we used an array of immunoinformatics approaches to design a multiepitope peptide-based vaccine against HCV by emphasizing 6 conserved epitopes from viral protein NS5B. …”
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Association of Leptin and Leptin receptor Gene polymorphisms with Insulin resistance in pregnant women: A cross-sectional study [version 3; peer review: 3 approved] by Usha Adiga, Tirthal Rai, Nandit Banawalikar

“…Introduction Leptin, along with its receptor, are linked with mechanisms affecting a diverse array of pregnancy-specific pathologies that include gestational diabetes and intrauterine growth restriction. …”
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Kinome Profiling by Maikel P. Peppelenbosch

“…To enable array analysis, consensus peptides for kinases are spot on a solid support. …”
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Conserved LIR-specific interaction of Sigma-1 receptor and GABARAP by Marius Wilhelm Baeken, Maximilian Christ, Daniel Schmitt, Wencke Trein, Heike Nagel, Albrecht Martin Clement, Hagen Körschgen, Christian Behl

“…Biochemically, we uncovered a GABARAP-σ1R interaction depending on this motif via peptide array analysis and confirmed this via immunoprecipitation, co-localization, and proximity ligation assays. …”
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In silico methods for immunogenicity risk assessment and human homology screening for therapeutic antibodies by Aimee E. Mattei, Andres H. Gutierrez, Soorya Seshadri, Jacob Tivin, Matt Ardito, Amy S. Rosenberg, William D. Martin, Anne S. De Groot

“…Although this perspective discusses the in-silico immunogenicity risk assessment for monoclonal antibodies, bi-specifics, multi-specifics, and antibody-drug conjugates, the analysis of additional therapeutic modalities such as enzyme replacement proteins, blood factor proteins, CAR-T, gene therapy products, and peptide drugs is also made available on the ISPRI platform.…”
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Novel Gene Clusters for Secondary Metabolite Synthesis in Mesophotic Sponge‐Associated Bacteria by Nuo Chen, Liwei Liu, Jingxuan Wang, Deqiang Mao, Hongmei Lu, Tânia Keiko Shishido, Shuai Zhi, Hua Chen, Shan He

“…ABSTRACT Mesophotic coral ecosystems (MCEs) host a diverse array of sponge species, which represent a promising source of bioactive compounds. …”
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EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation by Bin Shi, Ping Yang, Huaijin Qiao, Jinchen He, Bin Song, Hao Bai, Fengdi Jiang, Yining Zhang, Qian Li, Tao Yan, Wenlin Tu, Daojiang Yu, Shuyu Zhang

“…Notably, VPS41 overexpression promotes KAI1 lysosomal degradation, protecting against radiation‐induced apoptotic cell death. Peptide array analysis pinpoints the VPS41‐KAI1 interaction through the K263 residue, consistent with AlphaFold prediction. …”
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CHD1 contributes to intestinal resistance against infection by P. aeruginosa in Drosophila melanogaster. by Johanna Sebald, Stefano Morettini, Valerie Podhraski, Cornelia Lass-Flörl, Alexandra Lusser

“…Infection experiments using different microbial species revealed defects in host defense in Chd1-deficient adults upon oral infection with P. aeruginosa but not upon septic injury, suggesting a so far unrecognized role for CHD1 in intestinal immunity. Further molecular analysis showed that gut-specific transcription of antimicrobial peptide genes was overactivated in the absence of infection in Chd1 mutant flies. …”
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Targeting ERBB3 and AKT to overcome adaptive resistance in EML4-ALK-driven non-small cell lung cancer by Josephina Sampson, Hyun-min Ju, Nan Zhang, Sharon Yeoh, Jene Choi, Richard Bayliss

“…Using phosphopeptide chip array and upstream kinase prediction analysis, we identified a group of phosphorylated tyrosine peptides including ERBB and AKT proteins that are upregulated upon ALK-TKI treatment in EML4-ALK-positive NSCLC cell lines. …”
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ARC/ARG3.1 binds the nuclear polyadenylate-binding protein RRM and regulates neuronal activity-dependent formation of nuclear speckles by Tambudzai Kanhema, Kamil Parobczak, Sudarshan Patil, Dagmara Holm-Kaczmarek, Erik I. Hallin, Jan Ludwiczak, Andrzej Antoni Szczepankiewicz, Francois Philippe Pauzin, Aamra Mahboob, Adrian Szum, Yuta Ishizuka, Stanisław Dunin-Horkawicz, Petri Kursula, Grzegorz Wilczynski, Adriana Magalska, Clive R. Bramham

“…We demonstrate endogenous ARC protein-protein interaction with polyadenylate-binding nuclear protein 1 (PABPN1) and the paraspeckles protein polypyrimidine tract-binding protein (PTB)-associated splicing factor (PSF). In vitro peptide binding arrays show direct binding of purified ARC to the PABPN1 poly(A)-RNA recognition motif. 3D morphometric imaging reveals structural changes in PABPN1 foci corresponding to classical nuclear speckles following in vivo and in vitro LTP. …”
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A portable biosensor for simultaneous diagnosis of TNF-<i>α</i> and IL-1<i>β</i> in saliva biomarkers using twin electronic devices by M. Monajjemi, F. Mollaamin, M. Dehghandar, S. Shahriari, P. Latifi, S. Mohammadi

“…This system works by arraying metal–oxide–semiconductor field-effect transistors (MOSFETs), with each targeting a biorecognition element (BRE) specific to one of these two important biomarkers. …”
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In vitro model of retinoblastoma derived tumor and stromal cells for tumor microenvironment (TME) studies by Emily Alefeld, André Haase, Dario Van Meenen, Bettina Budeus, Oliver Dräger, Natalia Miroschnikov, Saskia Ting, Deniz Kanber, Eva Biewald, Nikolaos Bechrakis, Nicole Dünker, Maike Anna Busch

“…We characterized both immortalized cell lines using a human oncology proteome array, immunofluorescence staining of different markers and in vitro cell growth analyses. …”
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Murine leukemia virus p12 tethers the capsid-containing pre-integration complex to chromatin by binding directly to host nucleosomes in mitosis. by Madushi Wanaguru, David J Barry, Donald J Benton, Nicola J O'Reilly, Kate N Bishop

“…Direct binding of MLV p12_M63I peptides to histones was confirmed by biolayer-interferometry (BLI) assays using highly-avid recombinant poly-nucleosomal arrays. …”
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