Optimizing Single‐Cell Measurement Using Dynamic Atomic Force Microscopy by Indrianita Lionadi, Gourav Bhattacharya, Liam McLarnon, George A. Burke, Amir Farokh Payam

“…Advancements in atomic force microscopy (AFM) have established it as a versatile tool for imaging and characterizing biological samples, including living single cells in physiological conditions. …”
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Optimized Denoising Method for Fourier Ptychographic Microscopy Based on Wirtinger Flow by Sining Chen, Tingfa Xu, Jizhou Zhang, Xing Wang, Yizhou Zhang

“…Fourier ptychographic microscopy (FPM) is a recently developed technique for high-resolution complex imaging with a wide field-of-view. …”
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A pilot study of short-duration sputum pretreatment procedures for optimizing smear microscopy for tuberculosis. by Peter Daley, Joy Sarojini Michael, Kalaiselvan S, Asha Latha, Dilip Mathai, K R John, Madhukar Pai

“…Further work is needed to confirm this in a larger study and also determine if longer duration pre-treatment might be effective in optimizing smear microscopy for TB.…”
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Research and Optimization of White Blood Cell Classification Methods Based on Deep Learning and Fourier Ptychographic Microscopy by Mingjing Li, Junshuai Wang, Shu Fang, Le Yang, Xinyang Liu, Haijiao Yun, Xiaoli Wang, Qingyu Du, Ziqing Han

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Calibration of Electron Microscopes Through Deep Learning and Bayesian Optimization by Jilles S. van Hulst, Roy A. C. van Zuijlen, Narges Javaheri, Maurits Diephuis, Duarte J. Antunes, W. P. M. H. Heemels

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Maximizing the biochemical resolving power of fluorescence microscopy. by Alessandro Esposito, Marina Popleteeva, Ashok R Venkitaraman

“…These strategies enable the optimal use of the information content available within the limited photon-budget typically available in fluorescence microscopy. …”
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Optimization of FIB milling procedure for micromechanical testing of cement pastes by Jan Procházka, Jiří Němeček, Patricie Halodová, Jiří Němeček

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FEATURES MORPHOLOGY ABNORMAL FORMS OF ERYTHROCYTES BY ATOMIC FORCE MICROSCOPY by Mohammed Aslanovich Selimov, Yegor Leonidovich Demchenkov, Andrew Ashotovich Nagdalian, Yuliana Sergeyevna Gatina

“…Annotation The possibility of using atomic force microscopy (AFM) to qualitatively assess the morphology and the visualization of abnormal forms of red blood cells (RBCs), in comparison with traditional methods of optical microscopy, was studied in this article. …”
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Optimal mix ratio and numerical simulation study of concrete modified by nano-silica synergistically reinforced with basalt fibers by Changxing Zhu, Duo Li, Shengran Zhang, Huazhe Jiao, Jiaqi Guo, Xinming Chen

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Accurate quantitative wide-field fluorescence microscopy of 3-D spheroids by Elizabeth Leary, Claire Rhee, Benjamin Wilks, Jeffrey R. Morgan

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Research on Reflective High-Speed Multi-Point Confocal Microscopy System by Huiqin Hu, Ning He, Kuangwei Du, Yukun Zhu, Dakai Zhou, Yuan Mi, Yunbo Chen, Junteng Ma, Youhua Chen, Cuifang Kuang

“…By integrating uniform illumination with microlens arrays and optimizing instantaneous structured illumination microscopy optical components, the system achieves a resolution of 1116 lp/mm (20×, 0.42), 100 fps imaging, and a 500 µm × 500 µm field of view while improving contrast. …”
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Combined FLIM, Confocal Microscopy, and STED Nanoscopy for Live-Cell Imaging by Magalie Bénard, Christophe Chamot, Damien Schapman, Alexis Lebon, Ludovic Galas

“…Maintaining cell survival by limiting light-induced cellular stress is challenging and requires protocol development and image acquisition optimization. Here, we provide a guide by considering the quartet sample, probe, instrument, and image processing to obtain appropriate resolutions and information for live cell fluorescence imaging. …”
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Assessing particle count in electron microscopy measurements of nanomaterials to support regulatory guidance by Charlotte Wouters, Vikram Kestens, Eveline Verleysen, Jan Mast

“…This legislation often requires risk assessments and thus reliable characterization data, for which regulatory guidance generally recommend electron microscopy. The guidance provides best practices for measurements but lacks requirements on how many particles to measure. …”
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Dimer ribbon structures on diamond (001) surfaces revealed with atomic force microscopy by Runnan Zhang, Yuuki Yasui, Masahiro Fukuda, Masahiko Ogura, Toshiharu Makino, Daisuke Takeuchi, Taisuke Ozaki, Yoshiaki Sugimoto

“…Formed in the nonequilibrium environment of plasma, these structures were evaluated as the most stable configurations through density-functional-theory calculations. Our findings provide a crucial foundation for optimizing the film growth process.…”
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Label-free determination of hemodynamic parameters in the microcirculaton with third harmonic generation microscopy. by Steffen Dietzel, Joachim Pircher, A Katharina Nekolla, Mazhar Gull, André W Brändli, Ulrich Pohl, Markus Rehberg

“…Comparison of the current microscopic resolution with theoretical, diffraction limited resolution let us conclude that an about sixty-fold THG signal intensity increase may be possible with future improved optics, optimized for 1200-1300 nm excitation. THG microscopy is compatible with simultaneous two-photon excited fluorescence detection. …”
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Cellpose as a reliable method for single-cell segmentation of autofluorescence microscopy images by Jeremiah M. Riendeau, Amani A. Gillette, Emmanuel Contreras Guzman, Mario Costa Cruz, Aleksander Kralovec, Shirsa Udgata, Alexa Schmitz, Dustin A. Deming, Beth A. Cimini, Melissa C. Skala

“…Abstract Autofluorescence microscopy uses intrinsic sources of molecular contrast to provide cellular-level information without extrinsic labels. …”
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Factorial-experimental investigation of LPBF regimes for VZh159 nickel superalloy grain structure and structural strength optimization by Rustam R. Kyarimov, Eugene S. Statnik, Eugene S. Statnik, Iuliia A. Sadykova, Alexander A. Frantsuzov, Alexey I. Salimon, Alexey I. Salimon, Alexander M. Korsunsky, Alexander M. Korsunsky, Alexander M. Korsunsky

“…This study provides new insights into the effects of LPBF parameters on the microstructure, particularly the formation of the γ′ strengthening phase and its correlation with mechanical performance. …”
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Diagnosis of Malaria among Children in Sokoto: A Comparison of Microscopy and Rapid Diagnostic Tests by Mustapha Umar Kalgo, B O P Musa, H I Inabo, Laila Hassan

“… • The findings highlight the higher positivity rate of microscopy, advocating for its continued use alongside RDTs to ensure accurate malaria diagnosis and optimal patient care in similar environments. …”
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In situ and operando transmission electron microscopy for the synthesis, fundamental understanding and catalytic applications of nanoalloys by Damien Alloyeau, Nathaly Ortiz Pena, Guillaume Wang, Christian Ricolleau, Jaysen Nelayah

“…Over the last two decades, the progresses of in situ and operando transmission electron microscopy (TEM) has revolutionized the way to meet this challenge by providing unparalleled observations of nanomaterial dynamics in their formation and application media. …”
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Correlative light and electron microscopy imaging of proteinaceous deposits in cell cultures and brain tissues by Peizhou Jiang, Dennis W. Dickson

“…Correlative light and electron microscopy (CLEM) has emerged as a powerful tool to accurately study tissue and cellular pathology by examination of the same target at both microstructural and ultrastructural levels. …”
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