Expanding structural insights into DNA packaging apparatus and endolysin LysSA05 function of Epsilon15 bacteriophage by Muhammad Saleem Iqbal Khan, Ju Wu, Shenlin Ji, Demeng Tan, Bingrui Sui, Shanshan Peng, Jinbiao Zhan, Jiajun Yin

“…Iodixanol-purified virions suspended in phosphate-buffered saline (PBS), under conditions optimized to preserve virion stability, were analyzed using graphene oxide (GO)-supported cryo-electron microscopy. …”
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Optimization of the endodontic retreatment by Z. S. Khabadze, F. R. Ismailov

“…Aim. Optimization of an endodontic retreatment by performing the following tasks, including the selection of an optimal and safe solvent.Materials and methods. …”
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Advanced Optical Microscopic Imaging Techniques for Imaging Amyloid Beta and Deciphering Alzheimer's Disease Pathogenesis by Shiju Gu, Chongzhao Ran

“…These techniques include fluorescence microscopy (FM), confocal microscopy (CM), two‐photon fluorescence microscopy (TPFM), super‐resolution microscopy (SRM), expansion microscopy (ExM), and light‐sheet fluorescence microscopy (LSFM). …”
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Apertureless Near-Field Scanning Probes Based on Graphene Plasmonics by Hamid T. Chorsi, John X. J. Zhang

“…The optimized probes feature full-width at half-maximum (FWHM) around 25 nm, which is at least two times smaller than conventional metallic plasmonic tips. …”
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Trail-blazing and keeping pace: building, retaining and expanding image analysis expertise by David Kirchenbuechler, David Kirchenbuechler, Mariana De Niz, Mariana De Niz, Constadina Arvanitis, Constadina Arvanitis

“…We hope that this shared experience can help others optimize their service though our lessons learned, and avoid pitfalls we faced during our Core’s timeline. …”
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A five-gene qPCR signature can classify type 2 asthma comparably to microscopy of induced sputum from severe asthma patients by B. Toennesen, J. M. Schmid, B. S. Sørensen, M. Fricker, H. J. H. Hoffmann

“…Characterization of airway inflammation is important to choose the optimal treatment for severe asthma patients eligible for biological treatment. …”
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X-Ray Photon Correlation Spectroscopy, Microscopy, and Fluorescence Recovery After Photobleaching to Study Phase Separation and Liquid-to-Solid Transition of Prion Protein Condensa... by Mariana Do Amaral, Aline Passos, Satabdee Mohapatra, Maria Freire, Susanne Wegmann, Yraima Cordeiro

“…Next, we detail strategies for using fluorescence microscopy to give insights into the folding state of proteins in condensates. …”
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Toxicity Assay and Pathogenic Process Analysis of <i>Clonostachys rogersoniana</i> Infecting <i>Cephalcia chuxiongica</i> by Junjia Lu, Jian Liu, Huali Li, Yajiao Sun, Shuwen Liu, Mengyao Wang, Yonghe Li

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Data stewardship and curation practices in AI-based genomics and automated microscopy image analysis for high-throughput screening studies: promoting robust and ethical AI applicat... by Asefa Adimasu Taddese, Assefa Chekole Addis, Bjorn T. Tam

“…The current challenges identified in AI-based data stewardship and curation practices are lack of infrastructure and cost optimization, ethical and privacy considerations, access control and sharing mechanisms, large scale data handling and analysis and transparent data-sharing policies and practice. …”
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Electrospun Nanofiber-Scaffold-Loaded Levocetirizine Dihydrochloride Cerosomes for Combined Management of Atopic Dermatitis and Methicillin-Resistant <i>Staphylococcus Aureus</i> (... by Rofida Albash, Samer Khalid Ali, Rehab Abdelmonem, Ahmed M. Agiba, Renad Aldhahri, Asmaa Saleh, Amira B. Kassem, Menna M. Abdellatif

“…An in vitro release assay was used to calculate the amount of LVC released from the LVC-NFs and the optimized CERs-NFs. For morphological assessment of NFs, LVC-NFs and CERs-NFs were subjected to transmission electron microscopy, scanning electron microscopy, and confocal laser scanning microscopy. …”
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Protocol for generating and characterizing a nasal epithelial model using imaging with application for respiratory viruses by Victor H.K. Lam, Aleena Ghafoor, Yazan Khan, Shirley Constable, Lane B. Buchanan, David Zuanazzi, Reeya Parmar, Zeynep G. Tepe, Leigh J Sowerby, Cindy M. Liu, Ryan M. Troyer, Jessica L. Prodger

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Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structures by Mariana Rebocho da Costa, Ana Ribeiro, Raquel Quitéria, Dalila Neves-Silva, Leonor Saúde

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Changes in Neutrophil Membranes Induced by Plasma from Newborns with Infection by V. A. Inozemtsev, I. V. Obraztsov, E. A. Sherstyukova, S. S. Kandrashina, M. A. Shvedov, M. E. Dokukin, V. A. Sergunova

“…A standardized protocol for neutrophil preparation and evaluation was developed. Optimal incubation conditions were established; 1% bovine serum albumin (BSA) yielded minimal background activation. …”
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Characterization of a Single-Capture Bright-Field and Off-Axis Digital Holographic Microscope for Biological Applications by Jian Kim, Álvaro Barroso, Steffi Ketelhut, Jürgen Schnekenburger, Björn Kemper, José Ángel Picazo-Bueno

“…The proposed imaging concept integrates a fiber-optic Mach–Zehnder interferometer-based off-axis digital holographic microscopy (DHM) with an inverted commercial optical BF microscope. …”
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Protocol for the isolation of brain microvessels and visualization of RNA fluorescence in mice and humans by Olivia M. Osborne, Oandy Naranjo, Silvia Torices, Sarah Schmidlin, Destiny Tiburcio, Minseon Park, Michal Toborek

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Microscopic insights into the structural and functional properties of organic perovskite materials and devices by Smruti Medha Mishra, Bhabani Swain, Abduk Kareem K. Soopy, Naga Venkateswar Rao Nulakani, Shanavas Shajahan, Inas Taha, Yarjan Abdul Samad, Adel Najar, Dalaver H. Anjum

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Pattern Matching-Based Denoising for Images with Repeated Sub-Structures by Anil Kumar Mysore Badarinarayana, Christoph Pratsch, Thomas Lunkenbein, Florian Jug

“…In electron microscopy, obtaining low-noise images is often difficult, especially when examining biological samples or delicate materials. …”
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Experimental study of manual and femtosecond laser-assisted methods for cutting limbal mini-transplants by B.E. Malyugin, S.A. Borzenok, O.N. Nefedova, D.S. Ostrovskii, M.Yu. Gerasimov, A.V. Shatskikh

“…At first step, performed on 3 porcine and 3 cadaveric eyes, the optimal energy parameters of FSL were selected, which ensured the excision of limbal mini-transplants. …”
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Preparation and Optimization of Vermiculite @ Starch Carbide Composites by Huicong DU, Kaifang WEI, Jingjing HUANG, Yunhe LIU, Wei WANG, Ping LAN, Lihong LAN

“…The results of scanning electron microscopy (SEM) and infrared spectroscopy (FTIR) showed that starch carbide was successfully loaded on the surface of vermiculite. …”
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Optimal experimental design for parameter estimation of a cell signaling model. by Samuel Bandara, Johannes P Schlöder, Roland Eils, Hans Georg Bock, Tobias Meyer

“…Here we utilized a numerical method to iteratively design optimal live-cell fluorescence microscopy experiments in order to reveal pharmacological and kinetic parameters of a phosphatidylinositol 3,4,5-trisphosphate (PIP(3)) second messenger signaling process that is deregulated in many tumors. …”
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