Comparison of methods for the quantification of cell-free DNA isolated from cell culture supernatant by Abel Jacobus Bronkhorst, Vida Ungerer, Stefan Holdenrieder

“…Analysis of the resulting data, along with an interpretation of theoretical values (i.e. the theoretical detection and quantification limits of the respective methods), enables the calculation of optimal conditions for several important preanalytical steps pertaining to each quantification method and different cell types, including the (1) time-point at which culture medium should be collected for cell-free DNA extraction, (2) amount of cell culture supernatant from which to isolate cell-free DNA, (3) volume of elution buffer, and (4) volume of cell-free DNA sample to use for quantification.…”
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Removal of hydrocarbons and heavy metals from petroleum water by modern green nanotechnology methods by Abderrhmane Bouafia, Souhaila Meneceur, Souheyla Chami, Salah Eddine Laouini, Henda Daoudi, Souheila Legmairi, Hamdi Ali Mohammed Mohammed, Narimene Aoun, Farid Menaa

“…In order to maintain biodiversity, hydrosphere ecosystems, and people, it is crucial to remove these heavy metals and polluting chemical compounds from the watery environment. In this study, different Nanoparticles (α-Fe2O3, CuO, and ZnO) were synthesized by green synthesis method using Portulaca oleracea leaf extract and characterized by UV–Vis spectrophotometers, FTIR spectroscopy, X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS) techniques in order to investigate morphology, composition, and crystalline structure of NPs, these were then used as adsorbent for the removal of As(III), Bi(II), Cd(II), Cr(VI), Mn(II), Mo(II), Ni(II), Pb(II), Sb(III), Se(-II), and Zn(II) from wastewater, and removal efficiencies of were obtained 100% under optimal conditions.…”
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Effect of Various Thermal Processing Methods on the Sensory, Textural, and Physicochemical Characteristics of Foal Meat by Renata Stanisławczyk, Jagoda Żurek, Mariusz Rudy, Marian Gil, Anna Krajewska, Dariusz Dziki

“…Previous research on the impact of various heat treatment methods on meat has primarily focused on conventional meats from common livestock animals, with limited studies on the effects of different heat treatments on foal meat. …”
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Historical and current aspects of angiovisualization methods in CАD (a literature review) by V. O. Hubka, Yu. M. Vaіlo, A. M. Materuhin, A. L. Makarenkov

“…Further research is needed on the efficacy and safety of different СA imaging methods in CAD. Better results of the diagnostic search depend on both the capabilities of the clinic hardware component and on the optimal sequence for diagnostic processes rationally constructed by physicians.…”
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Electric field variations across DLPFC targeting methods in TMS therapy for Alzheimer’s disease by Nianshuang Wu, Yuxuan Shao, Zhen Wu, Shuxiang Zhu, Penghao Wang, Ziyan Zhu, Cheng Zhang, Changzhe Wu, Xiaolin Huo, Hua Lin, Guanghao Zhang

“…Objective: This study assesses differences in TMS-induced E-field using functional versus anatomical targeting methods for DLPFC stimulation. …”
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Cleaning methods for biosafety cabinet to eliminate residual mycoplasmas, viruses, and endotoxins after changeover by Mitsuru Mizuno, Saeri Kimbara, Hanae Ichise, Natsumi Ishikawa, Yuto Nishihara, Miwako Nishio, Ichiro Sekiya

“…Moreover, although several methods for expunging these contaminants have been proposed, an optimal method has not yet been determined. …”
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Battery Cell Charging Behavior Analysis Using Constant Current and Constant Voltage Methods by Anna Cyntia, Muhammad Imran Hamid, Ayu Elsa Afriyanti

“…The simulation analyzes the charging efficiency, usable capacity, and internal impedance variation under various current levels and voltage thresholds. The CC/CV method is compared with findings from other studies that also used the CC/CV charging technique, highlighting similarities and differences in the results. …”
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Comparative analysis of data transformation methods for detecting non-technical losses in electricity grids by Maria Gabriel Chuwa, Daniel Ngondya, Rukia Mwifunyi

“…Six encoding techniques for time series data were evaluated: Markov transition fields (MTF), Gramian angular summation field (GASF), Gramian angular difference field (GADF), Recurrence plots (RP), and time–frequency analysis methods, including short-time Fourier transform (STFT) and continuous wavelet transform (CWT). …”
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Slope Stability Monitoring Methods and Technologies for Open-Pit Mining: A Systematic Review by Rohan Le Roux, Mohammadali Sepehri, Siavash Khaksar, Iain Murray

“…Using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) 2020 guidelines, a total of 49 studies were selected from a collection of four engineering databases, and a comparative analysis was conducted to determine the underlying differences between the various methods for open-pit slope stability monitoring in terms of their performance across key attributes, such as monitoring accuracy, spatial and temporal coverage, operational complexity, and economic viability. …”
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TRIPOD statement: a preliminary pre-post analysis of reporting and methods of prediction models by Gary S Collins, Karel G M Moons, Lotty Hooft, Ewout W Steyerberg, Pauline Heus, Merel van Diepen, Friedo W Dekker, Amir H Zamanipoor Najafabadi, Chava L Ramspek, Wilco C Peul

“…Objectives To assess the difference in completeness of reporting and methodological conduct of published prediction models before and after publication of the Transparent Reporting of a multivariable prediction model for Individual Prognosis Or Diagnosis (TRIPOD) statement.Methods In the seven general medicine journals with the highest impact factor, we compared the completeness of the reporting and the quality of the methodology of prediction model studies published between 2012 and 2014 (pre-TRIPOD) with studies published between 2016 and 2017 (post-TRIPOD). …”
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Difference in recurrence frequencies of non-muscle-invasive-bladder tumors depending on optimal usage of intravesical immunotherapy of bacillus Calmette-Guérin by Milošević Radovan, Milović Novak, Aleksić Predrag, Lazić Miodrag, Cerović Snežana, Bančević Vladimir, Košević Branko, Marić Predrag, Spasić Aleksandar, Simić Dejan, Kovačević Božidar

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Enhanced Global Optimization Using a Novel Hybrid Sine Cosine-Gazelle Algorithm with Brownian Motion and Lévy Flight Mechanisms by Gyan Singh, Saptadeep Biswas, Absalom El-Shamir Ezugwu, Vladimir Simic, Uttam Kumar Bera, Kashif Saleem, Laith Abualigah

“…Furthermore, employing the Combined Compromise Solution (CoCoSo) method for multi-criteria decision-making enables systematic ranking and comparison of HSCAGOA’s performance against other algorithms. …”
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Vicugna pacos semen collection methods and review of semen parameters using Triladyl ® diluent by Juan Ramón García-Díaz, Rafael Alfonso Garzón Jarrín, Luis Alonso Chicaiza Sánchez, Blanca Jeaneth Villavicencio Villavicencio

“…Libido and method acceptance did not differ between methods. …”
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Assessment of the radiant emittance of damaged/contaminated dental light-curing tips by spectrophotometric methods by Abdulrahman A. Balhaddad, Isadora Garcia, Fabrício Collares, Cristopher M. Felix, Nisha Ganesh, Qoot Alkabashi, Ward Massei, Howard Strassler, Mary Anne Melo

“…The radiant emittance was recorded using 3 spectrophotometric methods: a laboratory-grade thermopile, a laboratory-grade integrating sphere, and a portable light collector (checkMARC).ResultsA significant difference between the laboratory-grade thermopile and the laboratory-grade integrating sphere was found when the radiant emittance values of the control or damaged/contaminated tips were investigated (p < 0.05), but both methods were comparable to checkMARC (p > 0.05). …”
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Anatomy Education and Training Methods in Oral Surgery and Dental Implantology: A Narrative Review by Carlo Barausse, Pietro Felice, Roberto Pistilli, Gerardo Pellegrino, Lorenzo Bonifazi, Subhi Tayeb, Irene Neri, Foteini-Dionysia Koufi, Antonietta Fazio, Maria Vittoria Marvi, Lucia Manzoli, Stefano Ratti

“…The present review examines common trends in oral and implant surgery training, focusing on how traditional methods like donated body dissection coexist with different and modern educational tools, and highlights the pros and cons of the different approaches in order to optimize training outcomes. …”
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Effects of thermal processing methods on the edible quality, nutrition, and metabolites of shrimp of Metapenaeus ensis by Miao Xiong, Hejiang Zhou, Rui-Xue Yu, Jun-Quan Chen, Shuangping Wang, Wen Xu, Yang Tian, Ling-Yan Su

“…Purines and heterocyclic amines produced in the boiling treatment group are the least harmful and healthiest heat treatment methods. Drying treatment is the most nutritious heat treatment method for ensuring flavour. …”
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DEVELOPMENT OF A NEW GENERATION OF TORNUM REX MINING GRAIN DRYERS AND THEIR DESIGN METHODS by V. Fitsyk

“…The materials of the article analyze the problems and methods of drying grain in modern grain dryers. In recent years, the mixed-flow dryer has been the subject of many studies on drying efficiency, dryer control and productivity improvement. …”
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Research Progress Analysis Regarding Rainfall-Runoff Relationships Based on Bibliometrics and Visual Methods by Yi Qi, Wang Ruifang, Dou Xiaodong, Gao Yuting, Wang Changhao, Yang Lu

“…Although the research methods and simulation techniques have become increasingly advanced, and accurate, follow-up research still needs to be optimized regarding the technical means of simulation testing and the method of applying artificial intelligence (AI) and 3S technology to build a practical regional runoff and confluence coupling model. ② Research studies on the key factors influencing rainfall-runoff relationships, namely surface morphology, rainfall characteristics, and soil properties, have continuously become more in-depth. …”
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Evaporation Dynamics and Dosimetry Methods in Numerically Assessing MDI Performance in Pulmonary Drug Delivery by Mohamed Talaat, Xiuhua Si, Jinxiang Xi

“…This study numerically examined the role of evaporation dynamics and dosimetry methods in assessing the efficiency of MDI delivery to different regions in a mouth–lung model extending to the eleventh generation (G11) of lung bifurcations. …”
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Research on Tongue Image Segmentation and Classification Methods Based on Deep Learning and Machine Learning by Bin Liu, Zeya Wang, Kang Yu, Yunfeng Wang, Haiying Zhang, Tingting Song, Hao Yang

“…In the tongue color classification stage, we introduce a ridge regression model that classifies tongue color based on the proportion of different pixel categories. Using this method, the classification accuracy reaches 91.80%. …”
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