The Prevalence, Distribution, and Clinicopathological Features of Seven Lung Cancer Actionable Driver Mutations in Taiwan by Yu‐Ching Lin, Tsung‐Ming Yang, Ting‐Yao Wang, Yu‐Hung Fang, Ming‐Shian Lu, Chin‐Kuo Lin, Yuan‐Yuan Jiang, Chia‐Hung Han, Jrhau Lung, Ying‐Huang Tsai, Ming‐Szu Hung

“…Results Among the 223 patients, the mutation frequencies detected by the optimized targeted sequencing assay were: EGFR 48.88%, KRAS 6.28%, PIK3CA 5.83%, NRAS and BRAF both 1.79%, MET 0.90%, and HER2 0.45%. …”
Get full text

Scientific land greening under climate change: Theory, modeling, and challenges by Jia-Na Chen, Zai-Chun Zhu, Sen Cao, Peng-Jun Zhao, Pei Liu, Da-Jing Li, Wei-Min Wang, Yue Chen

“…This review underscored the importance of proactively implementing land greening programs and provided guidelines for scientific greening based on cutting-edge theory and methods.…”
Get full text

Aging, HIV infection, and alcohol exert synergist effects on regional thalamic volumes resulting in functional impairment by Adolf Pfefferbaum, Natalie M. Zahr, Stephanie A. Sassoon, Rosemary Fama, Manojkumar Saranathan, Kilian M. Pohl, Edith V. Sullivan

“…Unknown is whether aging with HIV affects thalamic nuclei and associated functions differentially and whether the common comorbidity of alcohol use disorder (AUD) + HIV accelerates aging. Methods: This mixed cross-sectional/longitudinal design examined 216 control, 69 HIV, and 74 HIV + AUD participants, age 25–75 years old at initial visit, examined 1–8 times. …”
Get full text

Neuropsychological outcome of indoor rehabilitation in post-COVID-19 condition—results of the PoCoRe study by Michael Jöbges, Melanie Tempfli, Melanie Tempfli, Christoph Kohl, Christoph Herrmann, Stefan Kelm, Alexa Kupferschmitt, Alexa Kupferschmitt, Alexa Kupferschmitt, Ida Montanari, Nike Walter, Gerhard Suetfels, Thomas Loew, Volker Köllner, Volker Köllner, Thilo Hinterberger

“…The PoCoRe study aimed to evaluate the burden of neuropsychological deficits in PCC patients undergoing multidisciplinary indoor rehabilitation and to describe possible changes in this symptomatology.MethodsThe PoCoRe study, a prospective, non-randomized, controlled longitudinal study, recruited PCC patients from six German indoor rehabilitation centers. …”
Get full text

Benefits of HIV‐1 transmission cluster surveillance: a French retrospective observational study of the molecular and epidemiological co‐evolution of recent circulating recombinant... by Marc Wirden, Fabienne Tombette, Sidonie Lambert‐Niclot, Marie‐Laure Chaix, Stéphanie Marque‐Juillet, Magali Bouvier‐Alias, Benedicte Roquebert, Moise Machado, Veronique Avettand‐Fenoel, Pierre Gantner, Enagnon Kazali Alidjinou, Karl Stefic, Jean‐Christophe Plantier, Vincent Calvez, Diane Descamps, Anne‐Genevieve Marcelin, Benoit Visseaux, the ANRS‐MIE resistance study group

“…This can also improve our knowledge of molecular and epidemiological factors for the optimization of prevention. Our objective was to illustrate this by studying the molecular and epidemiological evolution of the cluster including the new circulating recombinant form (CRF) 94_cpx of HIV‐1, detected in 2017 and targeted by preventive actions in 2018. …”
Get full text

Genetic polymorphism of msp2 in Plasmodium falciparum isolates among asymptomatic malaria infections from two ecological settings in Cameroon by Aline G. Bouopda-Tuedom, Luc Abate, Brice L. Feufack-Donfack, Christelle M. Ngou, Albert N. Bayibeki, Carole E. Eboumbou Moukoko, Lawrence S. Ayong, Antoine Berry, Thierry Lefèvre, Isabelle Morlais, Sandrine E. Nsango

“…Methods Dried blood spots collected from asymptomatic individuals in 2018 and 2019 were used to determine the Plasmodium infection status and distinguish the Plasmodium spp. by real-time PCR. …”
Get full text

Immunological insights into the occurrence of Lawsonia intracellularis in horses from southern Brazil using flow cytometry by Yasmin Ampese Matté, Débora Zini Baldasso, Mariana Antunes Rezende, Jean Francisco Maldaner Lui, Ana Clara Seibel, João Antônio Guizzo, Rafael Frandoloso, Luiz Carlos Kreutz

“…FCAT was a sensitive and specific diagnostic tool for detecting anti-L. intracellularis antibodies in horses. …”
Get full text

Fc-effector functional antibody assays for SARS-CoV-2 variants of concern by Xuemin Chen, Grace Li, Caroline Ciric, Theda Gibson, Larry J. Anderson, Larry J. Anderson, Christina A. Rostad, Christina A. Rostad

“…This study evaluated the effect COVID-19 XBB.1.5 booster vaccination on Fc-effector antibody responses to SARS-CoV-2.MethodsWe developed four assays to evaluate the Fc-effector functions of SARS-CoV-2 antibodies. …”
Get full text

Evaluating the potential of graphene oxide to promote skeletal muscle complex regeneration by Yulu Chen, Zeyu Zhu, Yian Shen, Xuling Liu, Yushi He, Chengqi Lyu, Jiayu Lu

“…Additionally, the effects of exosomes derived from GO-treated C2C12 myoblasts on osteoblasts were explored.MethodsC2C12 cells were cultured with different concentrations (0.1, 0.5, 2.5, 12.5, and 62.5 μg/mL) and particle sizes (>500 and <500 nm) of GO. …”
Get full text

Synergistic AI-resident approach achieves superior diagnostic accuracy in tertiary ophthalmic care for glaucoma and retinal disease by Dalia Camacho-García-Formentí, Gabriela Baylón-Vázquez, Karen Arriozola-Rodríguez, Enrique Avalos-Ramirez, Curt Hartleben-Matkin, Hugo Valdez-Flores, Damaris Hodelin-Fuentes, Alejandro Noriega

“…We present a Mexican AI-powered screening tool and evaluate it against first-year ophthalmology residents in a tertiary care setting in Mexico City.MethodsWe analyzed data from 435 adult patients undergoing their first ophthalmic evaluation using an AI-based platform and first-year ophthalmology residents. …”
Get full text

The role of supine imaging in assessing failure of pelvic compensation and its impact on surgical outcomes in patients with adult spinal deformity by Dongfan Wang, Wei Wang, Yu Wang, Chao Kong, Shuaikang Wang, Xiaolong Chen, Shibao Lu

“…Abstract Aims This study aims to investigate the impacts of failure of pelvic compensation (FPC), as determined by variations in spinopelvic alignment during the transition from the supine to the upright position, on surgical outcomes in patients with adult spinal deformity (ASD). Methods A total of 125 ASD patients who underwent posterior correction surgery with a minimum follow-up of two years were enrolled. …”
Get full text

Transforming apricot kernel shell into a multifunctional photocatalyst for wastewater treatment and antimicrobial applications by Faqin Lian, Fareena Batool, Muzammil Anjum, Samia Qadeer, Abubakr M. Idris, Waqar-un- Nisa, Zepeng Rao, Zepeng Rao, Habib Ullah

“…Among various industries, pharmaceutical compounds have been detected in various aquatic environments and food supply chains; therefore, they need an economical and efficient treatment process. …”
Get full text

Predictive Value of Varicocele Grade and Histopathology in Simultaneous Varicocelectomy and Sperm Retrieval in Non-Obstructive Azoospermia: A Retrospective Cohort Study by Aris Kaltsas, Fotios Dimitriadis, Michael Chrisofos, Nikolaos Sofikitis, Athanasios Zachariou

“…These findings provide valuable insights for optimizing fertility treatments in men with NOA undergoing varicocele repair.…”
Get full text

Novel Classification of Peroneal Tendon Disease Reveals Cellular and Molecular Phenotypes of the Tenosvnovium by Julia Matthias MD, Michael A. David PhD, Sara E. Buckley DO, Daniel J. Ross MD, Nathaniel Zona BA, Michael A. Hewitt B.A., Joshua Metzl MD, Daniel K. Moon MD, MS, MBA, Courtney Grimsrud MD, Douglas J. Adams PhD, Michael Zuscik PhD, Kenneth Hunt MD

“…We aimed to develop a clinically meaningful classification system for PTD incorporating detailed analysis of molecular, cellular, and tissue changes of the tenosynovium surrounding the peroneal tendon. Methods: Patients undergoing surgery for PTD (IRB-approved) were classified using our 5-type classification system (Fig 1A). …”
Get full text

Infections and antibiotic resistance in Malawian children 6-59 months old hospitalized with severe acute malnutrition by Dr Bryan Vonasek, Ms. Mwawi Nyirongo, Mr. Alexander Kondwani, Dr. Anthony Garcia-Prats, Ms. Tiwonge Kamvaunamwali, Ms. Doris Ng'oma, Mr. James Kaphatika, Mr. Amazing-Grace Tepeka, Dr. Mercy Kumwenda, Dr. Emily Ciccone, Dr. Tisungane Mvalo

“…The aim of this study was to give a contemporary description of the prevalence of treatable infections and AMR in children hospitalized with SAM. Methods: This prospective cohort study enrolled children 6-59 months old hospitalized with SAM at a tertiary referral hospital in Malawi. …”
Get full text

Mutation-based, neoadjuvant treatment for advanced anaplastic thyroid carcinoma by Sabine Wächter, Detlef K. Bartsch, J. Riera Knorrenschild, Anika Pehl, Friederike Eilsberger, Andreas Pfestroff, Markus Luster, Katharina Holzer, Andreas Neubauer, Elisabeth Maurer

“…This study aimed to investigate whether mutation-based neoadjuvant therapy can convert an initially unresectable tumor into a resectable state, optimizing local tumor control and prolonging overall survival.MethodsPatients with stages IVB and limited IVC BRAFV600E-negative ATC received immediate combination therapy consisting of the multikinase inhibitor (mKI) lenvatinib and the immune checkpoint inhibitor (ICI) pembrolizumab upon diagnosis. …”
Get full text

AI-HOPE-TGFbeta: A Conversational AI Agent for Integrative Clinical and Genomic Analysis of TGF-β Pathway Alterations in Colorectal Cancer to Advance Precision Medicine by Ei-Wen Yang, Brigette Waldrup, Enrique Velazquez-Villarreal

“…Methods: AI-HOPE-TGFbeta utilizes a large language model (LLM), Large Language Model Meta AI 3 (LLaMA 3), a natural language-to-code interpreter, and a bioinformatics backend to automate statistical workflows. …”
Get full text

Balancing accuracy and user satisfaction: the role of prompt engineering in AI-driven healthcare solutions by Mini Han Wang, Mini Han Wang, Mini Han Wang, Mini Han Wang, Mini Han Wang, Xudong Jiang, Xudong Jiang, Xudong Jiang, Xudong Jiang, Peijin Zeng, Peijin Zeng, Peijin Zeng, Xinyue Li, Kelvin Kam-Lung Chong, Guanghui Hou, Xiaoxiao Fang, Yang Yu, Xiangrong Yu, Junbin Fang, Yi Pan

“…This study explores the application of IoT-enabled, AI-driven systems for detecting and managing Dry Eye Disease (DED), emphasizing the use of prompt engineering to enhance system performance.MethodsA specialized prompt mechanism was developed utilizing OpenAI GPT-4.0 and ERNIE Bot-4.0 APIs to assess the urgency of medical attention based on 5,747 simulated patient complaints. …”
Get full text

Impact of respiratory syncytial virus on Streptococcus pneumoniae resistance to β-lactam antibiotics: an investigative study in children by Lu Li, Yushun Wan, Yuncheng Wang, Ye Tao, Xiao Long, Enmei Liu, Yu Deng

“…Abstract Background This study characterizes the molecular interplay between respiratory syncytial virus (RSV) glycoproteins (G/F) and Streptococcus pneumoniae (S.pn) penicillin-binding proteins (PBPs), while evaluating RSV’s potential role in modulating S.pn β-lactam resistance. Methods Protein docking and pull-down assays assessed G/F-PBP interactions. …”
Get full text

Does Syndesmotic Fixation Technique Impact Complication Rates and Functional Outcomes Measured by PROMIS Scores Following Operative Repair of Ankle Fractures? by Steven Hadley BA, Rachel Bergman MD, John Peabody MD, Sarah Westvold MPH, Ryan Filler MD, Milap Patel DO, Anish Kadakia MD

“…We hypothesized that Tightrope and IB would reduce complications and improve PROMIS scores compared to screws. Methods: 782 patients who underwent ankle fracture surgery at a single institution between January 2016-December 2021 were reviewed retrospectively. …”
Get full text