Protocol update to: High-throughput scNMT protocol for multiomics profiling of single cells from mouse brain and pancreatic organoids by Santiago Cerrizuela, Oguzhan Kaya, Lukas P.M. Kremer, Andrea Sarvari, Tobias Ellinger, Jannes Straub, Jan Brunken, Andrés Sanz-Morejón, Aylin Korkmaz, Ana Martín-Villalba

“…Summary: Single-cell nucleosome, methylome, and transcriptome (scNMT) sequencing is a recently developed method that allows multiomics profiling of single cells. …”
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Research on APF-Dijkstra Path Planning Fusion Algorithm Based on Steering Model and Volume Constraints by Xizheng Wang, Gang Li, Zijian Bian

“…Again, the three-point arc method based on the steering model is used to determine the location of the predicted points and to smooth the paths in real time while constraining the steering angle. …”
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Breast Cancer Screening Using a Modified Inertial Projective Algorithms for Split Feasibility Problems by Pennipat Nabheerong, Warissara Kiththiworaphongkich, Watcharaporn Cholamjiak

“…To detect breast cancer in mammography screening practice, we modify the inertial relaxed CQ algorithm with Mann’s iteration for solving split feasibility problems in real Hilbert spaces to apply in an extreme learning machine as an optimizer. …”
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Modular and efficient assembly of CRISPR vectors and construction of an apple gene editing efficiency evaluation system by HOU Feifan, HUANG Chuanmi, HAO Pengbo, CONG Liu, BAI Tuanhui, SHI Jiangli, WANG Miaomiao, LIU Yu, ZHAO Yujie, WAN Ran, SHEN Yawen, ZHANG Hengtao, ZHANG Kunxi, ZHENG Xianbo, JIAO Jian

“…[Methods] The conventional vectors pUC19 (DNA cloning plasmid) and pHK2 (Dicotyledonous gene editing plasmid based on pCAMBIA1300 modification) were firstly modified by inserting a pair of type Ⅱ S restriction enzyme sites (such as Esp3 Ⅰ or Bsa Ⅰ etc.) into selected positions, to construct the intermediate vector pUC19-Esp3 Ⅰ-Bb and the plant expression vector backbone pHK2-Bsa Ⅰ-Bb. …”
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Assessing the deep learning based image quality enhancements for the BGO based GE omni legend PET/CT by Meysam Dadgar, Amaryllis Verstraete, Jens Maebe, Yves D’Asseler, Stefaan Vandenberghe

“…Conclusion This study conducted a thorough evaluation of deep learning algorithms in the GE Omni Legend PET/CT scanner, demonstrating that these methods enhance image quality, with notable improvements in CRC and CNR, thereby optimizing lesion detectability and offering opportunities to reduce image acquisition time.…”
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Clinical features of patients with ankylosing spondylitis with inefficacy of two or more biological disease modifying antirheumatic drugs by Sh. F. Erdes, K. V. Sakharova, T. V. Dubinina, M. V. Cherkasova

“…The “Treat-to-target” (T2T) strategy for axial spondyloarthritis (axSpA) has made it possible to optimize therapy in most patients, but in some of them the treatment goals are not achieved.Objective: to analyze the clinical features of patients with ankylosing spondylitis (AS) with inefficacy of two or more biological disease modifying antirheumatic drugs (bDMARDs).Material and methods. …”
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Comparing the effectiveness of different exercise interventions on quality of life in stroke patients: a randomized controlled network meta-analysis by Liqun Jiang, Huimin Ding, Qishuai Ma, Shang Gao, Xinxin Zhang, Buongo Chun

“…Conclusion This study indicates that different training methods have a significant impact on various dimensions of quality of life in stroke patients. …”
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Comparative Forced Degradation Study of Anticomplement C5 Biosimilar and Originator Monoclonal Antibodies by Merve Celik Yamaci, Ceren Pamukcu, Yigit Erdemgil, Ahmet Emin Atik, Zeynep Zulfiye Yildirim Keles, Ozge Can

“…The products were then tested at defined time points using validated analytical methods. <b>Methods</b>: This study employed size-exclusion chromatography to detect aggregated forms. …”
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Development of a Discrete Algorithm for Interpreting Ground-Penetrating Radar Data in Vertically Heterogeneous Media by Kazizat Iskakov, Almaz Tatin, Natalya Glazyrina, Ainur Kussainova, Nurgul Uzakkyzy, Kakim Sagindykov

“…The inverse problem reconstruction of dielectric permittivity is considered a non-destructive method for radargram interpretation. Assuming a piecewise-continuous medium structure eliminates the need for computing gradients at material interfaces. …”
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Modern Trends in Imaging X: Spectral Imaging in Preclinical Research and Clinical Pathology by Richard Levenson, Joseph Beechem, George McNamara

“…Spectral imaging methods are attracting increased interest from researchers and practitioners in basic science, pre-clinical and clinical arenas. …”
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Measuring the rate of NADPH consumption by glutathione reductase in the cytosol and mitochondria. by Kenneth K Y Ting, Eric Floro, Riley Dow, Jenny Jongstra-Bilen, Myron I Cybulsky, Jonathan V Rocheleau

“…Furthermore, NADPH pools are compartmentalized in various organelles with no known membrane transporters, suggesting that NADPH-dependent pathways are regulated in an organelle-specific manner. Conventional methods such as commercial kits are limited to quantifying NADPH in whole cells and not at the resolution of specific organelles. …”
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Results of a Prospective Study Concerning the Clinical Efficiency of Recent Russian-Made Specialized Foods without Phenylalanine by T. V. Bushueva, T. E. Borovik, K. S. Ladodo, L. M. Kuzenkova

“…The chemical composition of the ration was controlled using dietology methods.Results: 57 children at the age of 14 days to 15 years with phenylketonuria (which was detected in neonatal screening) were picked for this study. …”
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Characterizing modifications to the mental health gap action programme (mhGAP) intervention guide during implementation in low- and middle-income countries using the framework for... by Harikeerthan Raghuram, Akanksha Jayant Rajguru, Mythili Menon Pathiyil, Aakrushi Brahmbhatt, Anant Bhan, Jessica Spagnolo, John A. Naslund

“…Methods This systematic review of reviews employs the Framework for Reporting Adaptations and Modifications-Expanded (FRAME) to document and characterize modifications to mhGAP implementation in LMICs. …”
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Influence of the thickness of Symbiotic Culture of Bacteria and Yeast on purification and final properties of bacterial cellulose by Federica Daus, Devis Montroni, Laura Pesavento, Martina Bruschi, Anna Liguori, Maria Letizia Focarete

“…This observation highlights the need to adapt the purification method based on SCOBY thickness.…”
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Mapping Polyclonal HIV-1 Antibody Responses via Next-Generation Neutralization Fingerprinting. by Nicole A Doria-Rose, Han R Altae-Tran, Ryan S Roark, Stephen D Schmidt, Matthew S Sutton, Mark K Louder, Gwo-Yu Chuang, Robert T Bailer, Valerie Cortez, Rui Kong, Krisha McKee, Sijy O'Dell, Felicia Wang, Salim S Abdool Karim, James M Binley, Mark Connors, Barton F Haynes, Malcolm A Martin, David C Montefiori, Lynn Morris, Julie Overbaugh, Peter D Kwong, John R Mascola, Ivelin S Georgiev

“…Computational neutralization fingerprinting, NFP, is an efficient and accurate method for predicting the epitope specificities of polyclonal antibody responses to HIV-1 infection. …”
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Soil eDNA-based droplet digital PCR assay as a new monitoring tool for terrestrial mammals by Marko Đokić, Vida Jojić, Pavle Lukić, Lucija Markulin, Vanja Bugarski-Stanojević

“…This study demonstrates the effectiveness of droplet digital PCR (ddPCR) for identifying vertebrate taxa from soil-derived environmental DNA (eDNA) a methodological combination not previously documented in literature. The protocol is optimized for detecting cryptic species of the lesser blind mole rat (BMR). …”
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BRAF in Melanoma: Pathogenesis, Diagnosis, Inhibition, and Resistance by Ryan J. Sullivan, Keith T. Flaherty

“…This paper provides an overview on the pathogenic ramifications of mutant BRAF signaling, the latest molecular testing methods to detect BRAF mutations, and the most recent clinical data of BRAF pathway inhibitors in patients with melanoma and BRAF mutations. …”
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Tracing Iridium Dissolution Pathways in Proton Exchange Membrane Water Electrolyzers at Relevant Current Densities in Real Time by Nico C. Röttcher, Jiahua Zhou, Lukas Löttert, Karl J. J. Mayrhofer, Dominik Dworschak

“…Discrepancies with so far reported findings from full‐cell and half‐cell experiments highlight a lack of understanding of catalyst dissolution and the transportation of dissolved species in different systems. The presented method offers unique insights which will help to study and optimize catalyst dissolution by means of various manufacturing and operation parameters to ultimately improve the stability of catalyst layers for water electrolysis.…”
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Case report: direct suture repair of inferior vena cava to rescue a stab patient with hepatic and caval injury through left hepatectomy and total vascular exclusion by Reina Hirooka, Kyoji Ito, Nobuyuki Takemura, Fuminori Mihara, Norihiro Kokudo

“…This report presents a method of vena caval repair in a case of penetrating retrohepatic IVC injury, requiring hepatic resection and total vascular exclusion (TVE). …”
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Refining Flow Cytometry-based Sorting of Plasma-derived Extracellular Vesicles by Daniele Reverberi, Maria Chiara Ciferri, Nicole Rosenwasser, Alessandro Catino, Giuseppina Poppa, Ilaria Giusti, Vincenza Dolo, Rodolfo Quarto, Sara Santamaria, Monica Colombo, Simona Coco, Roberta Tasso

“…Plasma EVs were isolated via sucrose cushion ultracentrifugation, optimizing purity and yield. Flow cytometry with fluorescence threshold triggering was fine-tuned to detect and sort CD9 + EVs, with instrument calibration and parameter adjustments mitigating swarming and improving sorting accuracy. …”
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