Exploring the role of hyperreflective walls as a biomarker for the management of cystoid macular edema by Zhang-Rong Yang, Chang Li, Dong-Jun Xing, Gao-Xu Wei, Ce-Ce Zhao, Miao Zhou, Xiao-Han Ma, Yi Zhao, Shu Yang, Rong-Guo Yu, Zhi-Qing Li

“…This study suggests that early detection of HRW and optimization of treatment strategies may improve patient prognosis.…”
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Can the Agent with Limited Information Solve Travelling Salesman Problem? by Tomoko Sakiyama, Ikuo Arizono

“…In our proposed algorithm, the agent cannot estimate tour lengths but detect only a few neighbor sites. Under the circumstances, the agent occasionally ignores the NN method (choosing the nearest site from current site) and chooses the other site far from current site. …”
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A Phos-tag-based approach reveals the extent of physiological endoplasmic reticulum stress. by Liu Yang, Zhen Xue, Yin He, Shengyi Sun, Hui Chen, Ling Qi

“…Despite its basic and clinical importance, the extent of ER stress inflicted by physiological and pathophysiological conditions remains difficult to quantitate, posing a huge obstacle that has hindered our further understanding of physiological UPR and its future therapeutic potential. Here we have optimized a Phos-tag-based system to detect the activation status of two proximal UPR sensors at the ER membrane. …”
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Orchard: Building large cancer phylogenies using stochastic combinatorial search. by Ethan Kulman, Rui Kuang, Quaid Morris

“…., cancer phylogenies, offer valuable insights about cancer development and guide treatment strategies. Many methods exist that reconstruct cancer phylogenies using point mutations detected with bulk DNA sequencing. …”
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Determination of Caffeine Content in Commercial Energy Beverages Available in Saudi Arabian Market by Gas Chromatography-Mass Spectrometric Analysis by Mohammed Al-Bratty, Hassan A. Alhazmi, Zia ur Rehman, Sadique A. Javed, Waquar Ahsan, Asim Najmi, Gulrana Khuwaja, Hafiz A. Makeen, Asaad Khalid

“…The method was successfully applied for the quantification of caffeine in energy drinks by directly injecting the multifold diluted samples in methanol. …”
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Stopping anticoagulation for isolated or incidental pulmonary embolism: the STOPAPE RCT protocol by Daniel Lasserson, Pooja Gaddu, Samir Mehta, Agnieszka Ignatowicz, Sheila Greenfield, Clare Prince, Carole Cummins, Graham Robinson, Jonathan Rodrigues, Simon Noble, Sue Jowett, Mark Toshner, Michael Newnham, Alice Turner

“…Subsegmental pulmonary embolism is not often detectable with older nuclear medicine-based diagnostic imaging for ventilation/perfusion mismatch. …”
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The clinical impact of 16S ribosomal RNA PCR and sequencing in the identification of bacterial infections: a 7-year report from a Lebanese tertiary care center by Nour Youssef, Nour Youssef, Celina F. Boutros, Fatima Dakroub, Fata Akl, Lina Reslan, Marc Finianos, Mohammad Bahij M. Moumneh, Tarek Bou Dargham, Zeinab El Zein, Zeinab El Zein, Zeinab El Zein, Amani Haddara, Rawan Korman, Sarah Khafaja, Sarah Khafaja, Sarah Khafaja, Ghassan Matar, Ghassan Matar, George F. Araj, George F. Araj, Ghassan S. Dbaibo, Ghassan S. Dbaibo, Ghassan S. Dbaibo

“…IntroductionThe identification of bacterial pathogens in the clinical setting is essential for providing optimal care and improving outcomes. The primary objective of this study was to assess the performance of the 16S test in bacterial identification from different samples and determine its impact on clinical outcomes.MethodsThis was a retrospective study of patient samples collected from all age-groups at the American University of Beirut Medical Center (AUBMC), from May 2016 to December 2022. …”
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Therapeutic and Diagnostic Tactics for Metastases to the Lymph Nodes of the Neck without an Identified Primary Focus by M. I. Sokolova, V. I. Pavlova

“…Metastatic squamous cell carcinoma from an unknown primary focus accounts for less than 5 % of malignant tumors in the head and neck. Improving the methods of diagnosis can reduce the incidence of this pathology. …”
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Inspection of railway catenary systems using machine learning with domain knowledge integration by Kacper Marciniak, Paweł Majewski, Jacek Reiner

“…Proposed methods substantially improve metrics such as AP50, precision, recall, and F1-score, particularly in detecting small and hard-to-spot catenary components such as insulators. …”
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Clinical and genetic characteristics of type 7 distal arthrogryposis caused by a pathogenic variant in the <i>MYH8</i> gene by I. V. Sharkova, S. S. Nikitin, T. V. Markova, A. E. Voskanyan, E. A. Melnik, O. A. Shchagina, E. L. Dadali

“…Exome sequencing after DNA isolation from the proband’s blood according to the standard method was carried out on the NextSeq 500 platform (Illumina, USA) using the pairedend reading method (2 × 75 bp). …”
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Extracellular vesicle digital scoring assay for assessment of treatment responses in hepatocellular carcinoma patients by Chen Zhao, Yi-Te Lee, Andrew Melehy, Minhyung Kim, Jacqueline Ziqian Yang, Ceng Zhang, Jina Kim, Ryan Y. Zhang, Junseok Lee, Hyoyong Kim, Yong Ju, Yuan-Jen Tsai, Xianghong Jasmine Zhou, Steven-Huy B. Han, Saeed Sadeghi, Richard S. Finn, Sammy Saab, David S. Lu, Jason Chiang, Jae-Ho Park, Todd V. Brennan, Steven A. Wisel, Manaf Alsudaney, Alexander Kuo, Walid S. Ayoub, Hyunseok Kim, Hirsh D. Trivedi, Yun Wang, Aarshi Vipani, Irene K. Kim, Tsuyoshi Todo, Justin A. Steggerda, Georgios Voidonikolas, Kambiz Kosari, Nicholas N. Nissen, Rola Saouaf, Amit G. Singal, Myung Shin Sim, David A. Elashoff, Sungyong You, Vatche G. Agopian, Ju Dong Yang, Hsian-Rong Tseng, Yazhen Zhu

“…Results HCC EV TR Scores, calculated from pre- and post-treatment plasma samples in the phase-2 case–control study, accurately differentiated post-treatment viable from nonviable HCC in the training (area under the ROC curve [AUROC] of 0.90, n = 49) and validation set (AUROC of 0.88, n = 51). At an optimal cutoff of 0.76 identified in the training set, HCC EV TR Score had high accuracy in detecting viable tumors (sensitivity: 76.5%, specificity: 88.2%) and found residual disease not initially observed on MRI in six patients with a median lead time of 63 days. …”
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Physicochemical and functional assessment of messenger RNA 5′Cap-end impurities under forced degradation conditions by J. Hutchinson, F. Schweikart, R. Shannon, A. Murthy, S.M. Bates, S. Leone, T. Nissan, D. Sideris, C. Lal, J. Button, R. Rani, G. Thom, N. Bond, E. Örnskov, S. Trabulo

“…Method-induced artifactual degradation occurred during LC-MS data acquisition, emphasizing the importance of using reference standards and optimizing methods to reduce such artifacts. …”
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Extensive cervical lesion and treatment outcomes in women with HIV/HPV co-infection by Rosie Mngqibisa, Huichao Chen, Catherine Godfrey, Motshedisi Sebitloane, Unoda Chakalisa, Sharlaa Badal-Faesen, Gaerolwe Masheto, Frank Taulo, Wadzanai Samaneka, Jennifer Tiu, Cynthia Firnhaber, Timothy Wilkin, the AIDS Clinical Trials Group A5282 Study Team

“…The Clopper-Pearson exact method was used to construct the 95% confidence interval for the proportion of WLHIV with lesions inappropriate for cryotherapy. …”
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Classifying Dry Eye Disease Patients from Healthy Controls Using Machine Learning and Metabolomics Data by Sajad Amouei Sheshkal, Morten Gundersen, Michael Alexander Riegler, Øygunn Aass Utheim, Kjell Gunnar Gundersen, Helge Rootwelt, Katja Benedikte Prestø Elgstøen, Hugo Lewi Hammer

“…Clinical signs and symptoms are currently used to diagnose dry eye disease. Metabolomics, a method for analyzing biological systems, has been found helpful in identifying distinct metabolites in patients and in detecting metabolic profiles that may indicate dry eye disease at early stages. …”
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SEVERE LIVER IRON CONCENTRATIONS (LIC) IN 24 PATIENTS WITH Β-THALASSEMIA MAJOR: CORRELATIONS WITH SERUM FERRITIN, LIVER ENZYMES AND ENDOCRINE COMPLICATIONS by Vincenzo De Sanctis

“…However, it causes iron-overload that requires monitoring and management by long-term iron chelation therapy in order to prevent endocrinopathies and cardiomyopathies, that can be fatal. Hepatic R2 MRI method (FerriScan®) has been validated as the gold standard for evaluation and monitoring liver iron concentration (LIC) that reflects the total body iron-overload. …”
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Recirculation in Veno-Venous Extracorporeal Membrane Oxygenation by Veronica Gagliardi, Giuseppe Gagliardi

“…As recirculation may significantly reduce extracorporeal oxygen delivery, leading to refractory hypoxemia, detecting and quantifying the recirculation fraction is fundamental in order to optimize VV-ECMO lung support. …”
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Bacterial single-cell RNA sequencing captures biofilm transcriptional heterogeneity and differential responses to immune pressure by Lee E. Korshoj, Tammy Kielian

“…Here, we present an optimized bacterial single-cell RNA sequencing method, BaSSSh-seq, to study Staphylococcus aureus diversity during biofilm growth and transcriptional adaptations following immune cell exposure. …”
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Identifying nontuberculous mycobacteria from a mycobacteria growth indicator tube with nuclear magnetic resonance spectroscopy: A laboratory metabolomics analysis case report by Dr Christoffel Opperman, Dr Marwaan Rylands, Dr Sarishna Singh, Ms Aysha Amien, Mr Yonas Ghebrekristos, Prof Rob Warren, Dr Wynand Goosen

“…However, the complexity of MGIT broth media presents challenges for NMR-based NTM identification, necessitating optimization of sample preparation methods. Therefore, in light of the growing demand for improved NTM diagnostic tools, this laboratory case accentuates the need to contemplate innovative approaches with cutting-edge technology for NTM identification.…”
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Early cerebrospinal fluid elevations of pTau-217 in severe traumatic brain injury subjects by Hamad Yadikar, Firas H. Kobeissy, Firas H. Kobeissy, Firas H. Kobeissy, Firas H. Kobeissy, Firas H. Kobeissy, Claudia Robertson, Spyridoula Tsetsou, John B. Williamson, John B. Williamson, Damon G. Lamb, Damon G. Lamb, Amy K. Wagner, Amy K. Wagner, Amy K. Wagner, Amy K. Wagner, Amy K. Wagner, Todd Kibaugh, Shih-Han Kao, Zhifeng Kou, Zhifeng Kou, Robert D. Welch, Jose-Miguel Yamal, Luis Leon-Novelo, Richard Rubenstein, Richard Rubenstein, Kevin K. W. Wang, Kevin K. W. Wang, Kevin K. W. Wang, Kevin K. W. Wang, Kevin K. W. Wang

“…This study investigated whether cerebrospinal fluid (CSF) phosphorylated Tau at threonine-217 (pTau-217) could serve as an early biomarker for severe TBI (sTBI).MethodsCSF samples from 26 sTBI patients, collected between 6 and 240 h post-injury, and 19 healthy controls were analyzed using an optimized direct enzyme-linked immunosorbent assay (ELISA; sensitivity &lt;4.7 pg/mL) for pTau-217 detection, complemented by Western blot validation. …”
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Proactive Frequency Stability Scheme Based on Bayesian Filters and Spectral Clustering by Gian Paramo, Mario D. Baquedano-Aguilar, Arturo Bretas, Sean Meyn

“…The proactive design of the method combined with its multiple layers of optimization deliver results that outperform state-of-the-art solutions. …”
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