A marine ship detection method for super-resolution SAR images based on hierarchical multi-scale Mask R-CNN by Jiancong Fan, Miaoxin Guo, Lei Zhang, Jianjun Liu, Jianjun Liu, Yang Li, Yang Li

“…Secondly, a hierarchical multi-scale Mask R-CNN (HMS-MRCNN) detection method is proposed, which significantly improves the multi-scale maritime ship detection problem through the cross-layer fusion of shallow features (small targets) and deep features (large targets). …”
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Design and assessment of a multiplex real-time PCR method for simultaneous detection and differentiation of COVID-19 and Influenza A/B by Nafiseh Fotros, Reihaneh Bashiri, Samira Mohammadi-Yeganeh, Mahdi Paryan

“…We developed a multiplex real-time PCR method to simultaneously detect Influenza A and B, as well as COVID-19. …”
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PCR-amplified Immunoassay (Immuno-PCR): Principle of the Method, Variants of Execution, Possibilities and Prospects of Use for the Detection of Pathogenic Biological Agents by A. S. Gorshkov, D. V. Pechenkin, A. V. Kuznetsovskiy, V. A. Balakin

“…The main methodological issues in the immuno-PCR technology are: the choice of a carrier of biomolecule complexes, the choice of a method for conjugation of detection antibodies and a reporter nucleic acid, optimization of methods for amplifying signal DNA and accounting for results, and development of methods for reducing background indicators. …”
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Establishment of a TaqMan qPCR method with MGB probe for the specific detection of BVDV field strains circulating in China by Lele An, Lele An, Xiaoting Ren, Yetao Zhang, Shubin Zhang, Yongqing Zhao, Yongqing Zhao

“…Universal primers and an MGB probe targeting the conserved 5′ untranslated region (5′UTR) of both BVDV-1 and BVDV-2 were designed based on complete genome sequences available in GenBank. Following optimization of the reaction conditions, the assay demonstrated a detection limit of 1.265 copies/μL using a plasmid standard. …”
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Development of a rapid on-site detection method for largemouth bass virus based on RPA-CRISPR/Cas12a system by Na Li, Chunmei Dai, Yuelin Mao, Yimeng Zhang, Huiyuan Yan, Huijiao Wang, Yinghui Qin, Baicheng Huang, Xinjie Wang, Lunguang Yao

“…Rapid, simple, and reliable detection methods are essential for the timely identification of LMBV infections, enabling effective prevention and control measures.MethodsIn this study, a detection platform utilizing the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12a (Cas12a) system was developed for LMBV. …”
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Early Forest Fire Detection With UAV Image Fusion: A Novel Deep Learning Method Using Visible and Infrared Sensors by Kunlong Niu, Chongyang Wang, Jianhui Xu, Jianrong Liang, Xia Zhou, Kaixiang Wen, Minjian Lu, Chuanxun Yang

“…Therefore, this research proposes a novel method to early forest fire detection by fusing visible and infrared images and creating a dataset. …”
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Green Microfluidic Method for Sustainable and High-Speed Analysis of Basic Amino Acids in Nutritional Supplements by Iva Pukleš, Csilla Páger, Nikola Sakač, Bojan Šarkanj, Dean Marković, Marija Kraševac Sakač, Marija Jozanović

“…The aim of this work was to develop a new method for the determination of L-arginine, L-ornithine, and L-lysine on low-cost microchip electrophoresis instrumentation conjugated with capacitively coupled contactless conductivity detection. …”
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Volume of Fluid (VOF) Method as a Suitable Method for Studying Droplet Formation in a Microchannel by Felipe Santos Paes da Silva, Paulo Noronha Lisboa-Filho

“…The VOF method provides a clearer understanding of transitions between fluid regimes by detecting stages of non-uniformity in droplet size. …”
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A quality-by-design optimized LC method for navigating degradation kinetics and quantification of favipiravir in the presence of degradation products and manufacturing impurities by Adel Ehab Ibrahim, Mohamed Farouk, Samy G. Alamir, Baher I. Salman, Tarek S. Belal, Sami El Deeb, Ahmed Al-Harrasi

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Analytical Methods for Atmospheric Carbonyl Compounds: A Review by Xiaoshuai Gao, Xin Zhang, Yan Nie, Jiemeng Bao, Junling Li, Rui Gao, Yunfeng Li, Wei Wei, Xiaoyu Yan, Yongxin Yan, Hong Li

“…Currently, offline analytical techniques for carbonyl compounds require significant time and advanced experimental skills for multiple optimization experiments to detect a broader range of species. …”
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Detection and Optimization of Traffic Networks Based on Voronoi Diagram by Rui Tao, Jian Liu, Yuqing Song, Rui Peng, Dali Zhang, Jiangang Qiao

“…We present a traffic peak detection method, which constructs the Voronoi diagram of the input traffic flow data and computes the prominence of candidate peak points using the diagram. …”
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Optimization and Validation of RP-HPLC-UV/VIS Method for Determination Some Antioxidants in Dry Calyces of Iraqi Hibiscus Sabdraffia Linn by Baghdad Science Journal

“…A new (Reversed Phase- High Performance Liquid chromatography) RP-HPLC method with Ultraviolet-Visible spectrophotometry has been optimized and validated for the simultaneous extraction and determination of antioxidants present in Iraqi calyces of Hibiscus Sabdraffia Linn. …”
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Adaptive weight optimization with large pretraining for pest detection by Kejian Yu, Wenwen Xu, Fuqin Geng, Yunzhi Wu

“…Herein, we propose an adaptive weight optimization method based on transfer learning for multimodal pest detection. …”
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A novel method integrating sample migration and threshold optimization for high-precision greenhouse classification: evidence from Southern China by Kaiyue Luo, Henggang Zhang, Chenhui Zhu, Tianyu Jiao, Alim Samat, Yonglin Chen, Chuanxiang Cheng

“…This study proposes a remote sensing classification framework using sample migration and dynamic threshold optimization for accurate, scalable greenhouse detection. …”
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An Enhanced Three-Dimensional Wind Retrieval Method Based on Genetic Algorithm-Particle Swarm Optimization for Coherent Doppler Wind Lidar by Xu Zhang, Xianqing Zang, Yuxuan Sang, Xinwei Lian, Chunqing Gao

“…In this paper, a wind retrieval method based on genetic algorithm-particle swarm optimization (GA-PSO) for the coherent Doppler wind lidar (CDWL) is proposed. …”
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Optimization of a thermochemical antibody stripping method for enhanced tyramide signal amplification-based opal multiplex immunohistochemistry in fragile tissues by Xiaotong Yu, Yan Song, Li Chen, Lixiang Xue, Jianling Yang, Chen Huang

“…Microwave treatment may compromise tissue integrity, additionally, the effectiveness of chemical reagents can be sensitive to variations in temperature, pH, and concentration. This study aims to optimize antibody stripping strategies specifically for use in Opal mIHC protocols, addressing limitations of current methods which particularly for tissues prone to delamination. …”
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Eco-friendly QbD-optimized chromatographic method for simultaneous analysis of metronidazole and nicotinamide with applications in permeation, stability, and sustainability evaluat... by Kanaka Parvathi Kannaiah, Mohamed A. M. Ali, Hemanth Kumar Chanduluru, Anis Ahmad Chaudhary, Mahmoud El-Maghrabey, Reem H. Obaydo, Galal Magdy, Mohamed A. El Hamd

“…A comparative assessment against 21 existing LC and UV methods revealed that the developed approach achieved the highest greenness score (0.75), required the least solvent volume (1.5 mL ethanol), and enabled sensitive detection of low-dose MTZ alongside high-dose NCT in semisolid gel matrices. …”
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Optimizing screening procedures for early detection of glycemic disorders by Inna Vladimirovna Misnikova, Alexander Vasil'evich Dreval, Yulia Alexandrovna Kovaleva, Valeria Alekseevna Gubkina, Tatiana Sergeevna Lakeeva

“…To estimate the actual prevalence of type 2 diabetes mellitus (T2DM) and prediabetes in individuals at high risk for T2DM and to develop an optimized stepwise screening procedure. Materials and Methods. …”
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Hybrid metaheuristic optimization for detecting and diagnosing noncommunicable diseases by Saleem Malik, S. Gopal Krishna Patro, Chandrakanta Mahanty, Saravanapriya Kumar, Ayodele Lasisi, Quadri Noorulhasan Naveed, Anjanabhargavi Kulkarni, Abdulrajak Buradi, Addisu Frinjo Emma, Naoufel Kraiem

“…H-GMRA outperforms traditional methods by identifying minimal feature sets with high dependency ratios. …”
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Optimization of bonding parameters in diffusion bonding of Cu10Sn-B4C composites produced by P/M method by Ada Hakan, Özsoy Serkan

“…With the increase in reinforcement rate, an increase in the shear strength of bonds and microhardness was observed. After the optimization process, the optimum result was detected in the bonding at 820°C temperature, 20 kg load, and 40 minutes duration in unreinforced bonding. …”
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