Latent infection of Caenorhabditis elegans by Orsay virus induces age-dependent immunity and cross-protection by Victoria G. Castiglioni, Ana Villena-Giménez, Dominik Herek, Antonio González-Sánchez, Christina Toft, Gustavo G. Gómez, Santiago F. Elena

“…By tracking the course of a lifelong infection, we demonstrate that the infection remains latent, with animals maintaining control over viral replication for most of their lifespan. Furthermore, we show that animals previously exposed to the virus can suppress viral replication following a second inoculation, indicative of an acquired immune response. …”
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Targeting neutrophil-driven inflammation in adult-onset still’s disease: molecular insights from gene expression profiles by In-Woon Baek, Hyoun-Ah Kim, Kyung-Su Park, Ki-Jo Kim

“…Therapeutic approaches aimed at neutrophils could offer a promising opportunity to regulate the inflammatory response in AOSD.…”
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<i>Tn</i>P and AHR-CYP1A1 Signaling Crosstalk in an Injury-Induced Zebrafish Inflammation Model by Geonildo Rodrigo Disner, Thales Alves de Melo Fernandes, Milton Yutaka Nishiyama-Jr, Carla Lima, Emma Wincent, Monica Lopes-Ferreira

“…Here, the inflammation model alone resulted in a significant response on the transcriptome, with most differentially expressed genes (DEGs) being upregulated across the groups. …”
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Xanthatin nanocrystals exert anti-inflammatory properties against TNFα-primed 2D monolayers and in 3D spheroids of human HT29 colorectal cancer cells by Aleksandra Berenguer Roque, Alain Zgheib, Suslebys Salomon-Izquierdo, Amanda Manso Peña, Luis A. Osoria Alfonso, Janet Piloto-Ferrer, Borhane Annabi

“…Of these inducers, HT29 cell response to TNFα resulted in the most elevated expression of cyclooxygenase (COX)-2 which was prevented by commercial xanthatin along with the phosphorylation of the extracellular signal-regulated kinase 1/2 and of IkappaB (IκB). …”
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Varying effects of chlorination on microbial functional repertoire and gene expression in contrasting effluents by Mandy Lok Yi Tang, Stanley Chun Kwan Lau

“…These enriched genes and transcripts were responsible for a wide range of functions, including energy generation, repair of damaged components and stress responses. …”
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New possibilities of pharmacotherapy for systemic lupus erythematosus: A place of belimumab by E. L. Nasonov

“…Various defects in the T cells regulating a B cell immune response have been detected. The development of genetic, epigenomic, transcriptomic, and proteomic technologies could identify a group of pathogenetically relevant cytokines, including BLyS (the B-lymphocyte stimulator is the most important component of cytokine-mediated regulation of B cell function, proliferation, and differentiation), interleukin (IL) 6, 17, 18, type 1 interferon, and tumor necrosis factor-α, which are involved in the development of visceral inflammation and damage.Large-scale clinical trials of different medications, primarily biological agents (BA), were conducted in patients with SLE. …”
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Revealing pathogenesis-associated metabolites in Histoplasma capsulatum through comprehensive metabolic profiling by Adrian Heckart, Jean-Christophe Cocuron, Stephanie C. Ray, Chad A. Rappleye, Ana P. Alonso

“…Metabolomic results of in vitro-grown yeasts were further characterized with available transcriptomic data, informing underlying gene expression patterns in response to contrasting milieus. …”
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Glypican-3 regulated epithelial mesenchymal transformation-related genes in osteosarcoma: based on comprehensive tumor microenvironment profiling by Jiaming Zhang, Wei Wang

“…IntroductionOsteosarcoma (OS) is the most common primary bone malignancy, predominantly affecting children and adolescents. …”
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Single-cell RNAseq and longitudinal proteomic analysis of a novel semi-spontaneous urothelial cancer model reveals tumor cell heterogeneity and pretumoral urine protein alterations... by Iliana K Kerzeli, Martin Lord, Milena Doroszko, Ramy Elgendy, Aikaterini Chourlia, Ivan Stepanek, Elinor Larsson, Luuk van Hooren, Sven Nelander, Per-Uno Malmstrom, Anca Dragomir, Ulrika Segersten, Sara M Mangsbo

“…The profiling of the model was performed by histology grading, single cell transcriptomic and proteomic analysis, while the derivation of a tumorigenic cell line was validated and used to assess in vivo anti-tumor effects in response to immunotherapy. …”
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Gastroesophageal circulating tumor cell crosstalk with peripheral immune system guides CTC survival and proliferation by Tania Rossi, Martina Valgiusti, Maurizio Puccetti, Giacomo Miserocchi, Michele Zanoni, Davide Angeli, Chiara Arienti, Ilaria Pace, Cristian Bassi, Ivan Vannini, Mattia Melloni, Erika Bandini, Milena Urbini, Massimo Negrini, Massimiliano Bonafè, Manuela Ferracin, Giulia Gallerani

“…Overall, we highlight how the immune system plays a key role in the proliferation of CTCs through EV signaling, and how CTC cell line genomic and transcriptomic alterations make these cells less visible from the immune system and likely responsible for the survival advantage in sites distant from the microenvironment of origin.…”
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7aaRGD - a novel SPP1/integrin signaling-blocking peptide reverses immunosuppression and improves anti-PD-1 immunotherapy outcomes in experimental gliomas by Aleksandra Ellert-Miklaszewska, Paulina Pilanc, Katarzyna Poleszak, Adria-Jaume Roura, Salwador Cyranowski, Mitrajit Ghosh, Szymon Baluszek, Maria Pasierbinska, Bartłomiej Gielniewski, Julian Swatler, Yuliana Hovorova, Kamil Wojnicki, Bozena Kaminska

“…Combining 7aaRGD with anti-PD-1 antibody resulted in reduced tumor growth, with an increase in the number of proliferating, interferon-ɣ producing CD8+T cells and depletion of regulatory T cells. Transcriptomic profiles of myeloid cells were altered by the combined treatment, reflecting the restored “hot” inflammatory TME and boosted immunotherapy responses. …”
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Integrative identification of key genes governing Verticillium wilt resistance in Gossypium hirsutum using machine learning and WGCNA by Yufeng Lei, Jing Zhao, Siyuan Hou, Fufeng Xu, Chongbo Zhang, Dongchen Cai, Xiaolei Cao, Zhaoqun Yao, Sifeng Zhao

“…In this study, we aimed to dissect the temporal transcriptional dynamics and regulatory mechanisms underlying Gossypium hirsutum response to V. dahliae infection.MethodsWe employed a time-course RNA-Seq approach using the susceptible upland cotton cultivar Jimian 11 to profile transcriptomic responses in root and leaf tissues post-V. dahliae inoculation. …”
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An in-depth exploration of four heterogeneity structure-based EGFR mutation subgroups in Chinese non-small cell lung cancer by Yongfeng Yu, Fei Yao, Jin Wang, Zhan Huang, Tao Hu, Changbin Zhu, Shun Lu

“…Furthermore, for the first time, we observed transcriptomic heterogeneity within these four subgroups. …”
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Glycosylation profiling of triple-negative breast cancer: clinical and immune correlations and identification of LMAN1L as a biomarker and therapeutic target by Qianru Yu, Hanyi Zhong, Xinhao Zhu, Chang Liu, Xin Zhang, Jiao Wang, Zongyao Li, Songchang Shi, Haoran Zhao, Cixiang Zhou, Qian Zhao

“…IntroductionBreast cancer (BC) is the most prevalent malignant tumor in women, with triple-negative breast cancer (TNBC) showing the poorest prognosis among all subtypes. …”
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Stratification system with dual human endogenous retroviruses for predicting immunotherapy efficacy in metastatic clear-cell renal cell carcinoma by Li Xu, Stephane Oudard, Catherine Sautes-Fridman, Xiaoping Su, Gabriel G. Malouf, Virginie Verkarre, Salma Kotti, Fangrong Yan, Yann-Alexandre Vano, Wolf Herve Fridman, Cheng-Ming Sun, Xiaofan Lu, Wenxuan Cheng

“…These subgroups were further consolidated into a three-tier risk model that significantly correlated with ICI treatment outcomes. The most responsive ERV risk category showed enhanced endothelial cell infiltration, whereas the resistant category was characterized by higher levels of myeloid dendritic cells, regulatory T cells, myeloid-derived suppressor cells, and markers of T-cell exhaustion. …”
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Linking a polyketide synthase gene cluster to 6-pentyl-alpha-pyrone, a Trichoderma metabolite with diverse bioactivities by Daniel Flatschacher, Alexander Eschlböck, Siebe Pierson, Ulrike Schreiner, Valentina Stock, Arne Schiller, David Ruso, Maria Doppler, Veronika Ruzsanyi, Mario Gründlinger, Christoph Büschl, Rainer Schuhmacher, Susanne Zeilinger

“…Surprisingly, the absence of pks1 led to enhanced lateral root formation in Arabidopsis thaliana during interaction with T. atroviride. Transcriptomic analysis revealed co-regulation of pks1 with adjacent genes, including candidates coding for a C3H1-type zinc finger protein and lytic polysaccharide monooxygenase, suggesting coordination between 6-PP biosynthesis and environmental response mechanisms. …”
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Unveiling tumor-infiltrating immune cell-driven immune-mediated drug resistance in clear cell renal cell carcinoma: prognostic insights and therapeutic strategies by Jifeng Yang, Yixuan Xing, Jiusong Luan, Wenbo Yang, Xin Zhang, Yanhua Tian, Haisong Zhang

“…Abstract Introduction Tumor drug resistance, particularly immune-mediated resistance, poses a significant challenge in cancer therapy, especially in clear-cell renal cell carcinoma (ccRCC), the most common and aggressive subtype of renal cancer. …”
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aFGF gene-modified adipose-derived mesenchymal stem cells promote healing of full-thickness skin defects in diabetic rats by Yiren Zhu, Pinhua Chen, Zhengchao Zhang, XueYi He, Ruoli Wang, Qi Fang, Zhixian Xu, Wubing He

“…Transcriptomic sequencing was performed to explore the underlying mechanisms driving enhanced wound healing. …”
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Modelling Toxoplasma gondii infection in human cerebral organoids by Hyang-Hee Seo, Hyo-Won Han, Sang-Eun Lee, Sung-Hee Hong, Shin-Hyeong Cho, Sang Cheol Kim, Soo Kyung Koo, Jung-Hyun Kim

“…We observed that tachyzoites can infect human cerebral organoids and are transformed to bradyzoites and replicate in parasitophorous vacuoles to form cysts, indicating that the T. gondii asexual life cycle is efficiently simulated in the brain organoids. Transcriptomic analysis of T. gondii-infected organoids revealed the activation of the type I interferon immune response against infection. …”
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Vitamin C potentiates the killing of Mycobacterium tuberculosis by bedaquiline through metabolic disruption by Catherine Vilchèze, Saranathan Rajagopalan, Raja Sab Kalluru, Niaz Banaei, William R. Jacobs

“…This combination also enhanced Mtb killing in infected human macrophages and peripheral blood mononuclear cells. Transcriptomic analysis revealed that the BDQ/vitamin C combination induces widespread metabolic disruption in Mtb, characterized by upregulation of stress response and metal ion homeostasis genes and downregulation of energy metabolism and cell wall biosynthesis genes. …”
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