Development and Validation of One-Step Reverse Transcription-Droplet Digital PCR for Plum Pox Virus Detection and Quantification from Plant Purified RNA and Crude Extract by Giorgia Bertinelli, Lorenza Tizzani, Marta Luigi, Simona Monticelli, Vincenza Ilardi

“…Plum pox virus (PPV) is the etiological agent of sharka, the most important viral disease of stone fruit worldwide. …”
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De Novo Genome Assembly and Annotation of the Arunachali Yak by Martina Pukhrambam, Kishor U. Tribhuvan, Vijay Paul, Avinash Pandey, Atrayee Dutta, Vijai Pal Bhadana, Suresh Dabas, G. I. Ramakrushna, K. P. Raghavendra, Pooja Verma, Y. G. Prasad, A. K. Mohanty, Sujay Rakshit, Mihir Sarkar

“…Annotation identified 25,855 protein-coding genes, with 81.5% functionally characterized. Repeat analysis revealed 44.68% transposable elements, with LINEs (28.26%) being the most abundant. …”
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Post-exposure vaccine protection of CTH522/CAF®01 against reinfection with Chlamydia trachomatis requires Th1/Th17 but not Th2-immunity by Nina Dieu Nhien Tran Nguyen, Sharmila Subratheepam, Safia Guleed, Kristoffer Mazanti Melchiors, Anja Weinreich Olsen, Katharina Wørzner, Frank Follmann, Jes Dietrich

“…Abstract Chlamydia trachomatis (C.t.) is globally the most common sexually transmitted bacterium with an estimated 131 million new cases occurring every year. …”
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Development of Microsatellite Markers for the Nipa Palm Hispid Beetle, Octodonta nipae (Maulik) by Zhiming Chen, Jun Chen, Xiang Zhang, Youming Hou, Guihua Wang

“…Based on existing transcriptome data, polymorphism simple sequence repeat (SSR) loci were identified. In total, 1274 SSR loci were identified from 49919 unigenes. …”
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A chromosome-level genome assembly of Coffea arabica L. var. ‘Kona Typica’ by Haomin Lyu, Jinjin Song, Yanbin Yin, Ming-Li Wang, Tracie Matsumoto, Vaishnavi Annabhemoju, Lyndel W. Meinhardt, Sunchung Park, Seunghyun Lim, Dapeng Zhang, Qingyi Yu

“…We annotated 65,458 protein-coding genes and identified 1,073,545 interspersed repeats, accounting for 65.16% of the genome. Analysis of transposon insertion ages revealed that most long terminal repeat retrotransposons proliferated after the polyploidization event. …”
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Stress-Relieving Effects of Japanese Green Tea: Evaluation Using the Molar Ratio of Caffeine and Epigallocatechin Gallate to Theanine and Arginine as an Indicator by Keiko Unno, Takashi Ikka, Hiroto Yamashita, Yoko Kameoka, Yoriyuki Nakamura

“…In addition, when the same tea leaves were infused repeatedly, it was found that most of the amino acids were eluted by the first and second cups; therefore, no stress-relieving effect could be expected after the third cup.…”
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A clinical case of recurrent pain syndrome due to hiatal hernia, occurring under the guise of acute pancreatitis by A. A. Baulin, L. A. Aver'yanova, V. A. Baulin, O. A. Baulina, D. A. Kompaneytseva

“…In the structure of urgent pathology diseases, the leading place belongs to acute pancreatitis, which is the third most common after acute appendicitis and acute cholecystitis. …”
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Treating recurrent hemarthrosis after knee arthroplasty with selective embolization: a cohort study of 56 patients by Suvi-Maria Sirola, Juuso Heikkinen, Pekka Kerimaa, Juho Kariniemi, Tuukka Niinimäki

“…Complications occurred in 7% of treatments, most of which resolved spontaneously. Conclusion: AE is effective in the treatment of recurrent hemarthrosis but 21% had reoccurance. …”
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Prolonged fever complicated by petechiae in an immunocompetent adult with primary CMV infection: A case report by Emilie Fraisse, Mads Emil Bruusgaard-Mouritsen, Rasmus Gregersen

“…Petechiae may be caused by CMV infection which is rarely considered in the differential diagnostic process. Most immunocompetent adults with primary CMV infection are asymptomatic or develop a mononucleosis-like syndrome with fever and mild hepatitis. …”
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DaapNLRSeek, prediction, and evolution of resistance genes in polyploid sugarcane genomes by Yiting Huang, Yingfeng Luo, Xiao Lin

“…However, pathogens cause significant yield losses. Most plant resistance (R) proteins are nucleotide-binding leucine-rich repeat (NLR) proteins, which detect pathogen effectors and activate immune responses. …”
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Robot-assisted low anterior resection after aluminum potassium sulfate and tannic acid sclerosing therapy for internal hemorrhoids by Yoshiro Itatani, Tomoaki Okada, Kenji Kawada, Koya Hida, Nobu Oshima, Susumu Inamoto, Rei Mizuno, Yoshihisa Okuchi, Yoshiharu Sakai

“…Abstract Background Internal hemorrhoids are the most common anal diseases. Aluminum potassium sulfate and tannic acid (ALTA) injection is a new sclerosing therapy for the treatment of internal hemorrhoids. …”
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ARTERIAL CHEMOINFUSION IN PATIENTS WITH LOCALLY ADVANCED AND METASTATIC PANCREAS CANCER by P. G. Tarazov, A. V. Kozlov, D. A. Granov, A. V. Pavlovskiy, A. A. Polikarpov, E. V. Rozengaus, S. A. Popov, E. V. Vlasova, V. E. Moiseenko

“…Symptomatic improvement showed the most patients. The objective response to the treatment had 20% patients and performance of repeated cycles led to increase of their survival.…”
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An improved machine-learning model for lightning-ignited wildfire prediction in Texas by Qi Zhang, Cong Gao, Chunming Shi

“…To address these limitations, we compiled a statewide dataset spanning 2010–2020 comprising 4775 LIWs and employed an optimized repeated-random undersampling strategy to mitigate class imbalance. …”
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Early and widespread cerebellar engagement during hippocampal seizures and interictal discharges by M.L. Streng, B.W. Kottke, E.M. Wasserman, L. Zecker, L. Luong, S. Kodandaramaiah, T.J. Ebner, E. Krook-Magnuson

“…We therefore implemented a novel method for repeated widefield calcium imaging of the cerebellum in awake, chronically epileptic mice. …”
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Enhancing causal inference in population-based neuroimaging data in children and adolescents by Rachel Visontay, Lindsay M. Squeglia, Matthew Sunderland, Emma K. Devine, Hollie Byrne, Louise Mewton

“…We identify just eight relevant studies, most of which employ propensity score-based methods. …”
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Assessment of Vault Particles in Cancer Cell Line‐Derived Extracellular Vesicle Preparations by Xinming Liu, Zubair Ahmed Nizamudeen, Christopher J. Hill, Christopher Parmenter, Kenton P. Arkill, Daniel W. Lambert, Stuart Hunt

“…RNase and proteinase treatment of DC‐derived pellets demonstrated that most vtRNA and MVP were not enclosed and protected within an EV membrane. …”
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Hemochromatosis and Heart Involvement by E. V. Reznik, M.H. E. Laouar, V. Yu. Voinova, G. N. Golukhov

“…The liver, pancreas, joints, skin, pituitary gland and heart are most often affected. Cardiac hemochromatosis is an important and potentially preventable cause of heart failure. …”
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Huntington’s Disease in a Patient Misdiagnosed as Conversion Disorder by João Machado Nogueira, Ana Margarida Franco, Susana Mendes, Anabela Valadas, Cristina Semedo, Gustavo Jesus

“…Usually the psychiatric disorders precede motor and cognitive impairment, Major Depressive Disorder and anxiety disorders being the most common presentations. We present a clinical case of a 65-year-old woman admitted to our Psychiatric Acute Unit. …”
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The Effectiveness of Retinalamin® Administered by Various Intramuscular Injection Timings in the Treatment of Patients with the Dry Form of Age- Related Macular Degeneration by V. V. Neroev, O. V. Zaitseva, T. D. Okhotsimskaya, I. V. Tsapenko, E. P. Lantukh

“…This is confirmed by a change in the P1 component of Mf ERG, which proved to be the most pronounced in the central ring, corresponding to the foveal area. …”
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The features of the mode of delivery in patients with congenital deficiency of coagulation factor VII: case reports by A. Yu. Bulanov, S. E. Rabotinsky, E. L. Bulanova, I. B. Simarova, Т. S. Kotomina, I. Yu. Sizova

“…The decision on the mode of delivery in patients with FVII deficiency is made according to obstetric criteria. In most cases, a single administration of rFVIIa is sufficient at a dose of 15–30 mcg/kg at the beginning of labor or before performing a caesarean section.…”
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