Shotgun metagenomic sequencing reveals the influence of artisanal dairy environments on the microbiomes, quality, and safety of Idiazabal, a raw ewe milk PDO cheese by Gorka Santamarina-García, Min Yap, Fiona Crispie, Gustavo Amores, Cathy Lordan, Mailo Virto, Paul D. Cotter

“…Results The results revealed diverse microbial ecosystems across sample types, comprising more than 1300 bacterial genera and 3400 species. …”
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Structure-dependent degradation of milk oligosaccharides by newly isolated intestinal commensal bacterial strains from suckling piglets and rabbits by Mathilde Rumeau, Sead Chadi, Frederic Pepke, Martin Beaumont, Cláudia M. Vicente, Agathe Juppeau, Céline Vandecasteele, Laurent Cauquil, Géraldine Pascal, Philippe Langella, Christelle Knudsen, Sylvie Combes, Rebeca Martín

“…Milk oligosaccharide fermentation generated a more diverse metabolome compared to glucose. …”
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Amniotic fluid-derived stem cells: potential factories of natural and mimetic strategies for congenital malformations by Cristiane S. R. Fonteles, Julia Enterria-Rosales, Ying Lin, John W. Steele, Ramiro A. Villarreal-Leal, Jing Xiao, Daniel I. Idowu, Beck Burgelin, Bogdan J. Wlodarczyk, Richard H. Finnell, Bruna Corradetti

“…The possibility to exploit both formulations as mRNA-therapeutics was explored by evaluating cell uptake in two different cell types(fibroblasts, and macrophages) and mRNA functionality overtime in an in vitro experimental setting as well as in an ex vivo, whole embryo culture using pregnant C57BL6 dams. …”
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Acridocarpus smeathmannii root extracts inhibit human prostate and bladder smooth muscle contraction, porcine arterial vasoconstriction, and cytotoxicity of prostate stromal cells by Oluwafemi Ezekiel Kale, Sheng Hu, Claudia Huber, Felix Schierholz, Anna Ciotkowska, Alexander Tamalunas, Christian G. Stief, Wolfgang Eisenreich, Martin Hennenberg

“…In addition, assays were conducted to evaluate cell proliferation and viability, providing complementary insights into functional and cellular-level effects. The bioactive compounds in the extract were characterized using gas chromatography–mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy and were subsequently evaluated in silico for their interaction with the α1-adrenergic receptor.ResultsProstate tissue contractions induced by α1-adrenergic agonists (0.1–100 µM) were reduced by 50% or more with A. smeathmannii at concentrations of 0.25 and 0.50 mg/mL. …”
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Deficiency of T cell CD40L has minor beneficial effects on obesity-induced metabolic dysfunction by Lisa Willemsen, Myrthe den Toom, Myrthe E Reiche, Marion J J Gijbels, Esther Lutgens, Bram van Os, Norbert Gerdes, Suzanne A B M Aarts

“…Our data indicate that other CD40L-expressing cell types are more relevant in the pathogenesis of obesity-associated metabolic dysfunction.…”
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Engineering adoptive T cell therapy to co-opt Fas ligand-mediated death signaling in ovarian cancer enhances therapeutic efficacy by Philip D Greenberg, Kristin G Anderson, Shannon K Oda, Breanna M Bates, Madison G Burnett, Magdalia Rodgers Suarez, Susan L Ruskin

“…Background In the USA, more than 50% of patients with ovarian cancer die within 5 years of diagnosis, highlighting the need for therapeutic innovations. …”
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Strong Hsp90α/β Protein Expression in Advanced Primary CRC Indicates Short Survival and Predicts Response to the Hsp90α/β-Specific Inhibitor Pimitespib by Sebastian B. M. Schmitz, Jakob Gülden, Marlene Niederreiter, Cassandra Eichner, Jens Werner, Barbara Mayer

“…The anti-cancer effect and chemosensitization of Pim-based therapy were prospectively confirmed in PDCS directly generated from Hsp90α/β > 40% pCRCs. Protein profiling combined with functional drug testing stratifies Hsp90α/β > 40% pCRC patients diagnosed with UICC IIb-IV for effective Pim-based therapy.…”
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Expectation Confirmation and Technology Acceptance: A Study of the Public's Attention to Accept the Government's Digital Human Avatar by Jinghao CHEN, Feng JIA, Qianxi LIU

“…[Purpose/Significance] In the digital age, government digital avatars represent a significant innovative application of the integration of generative artificial intelligence and digital government. …”
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A Monocyte-Driven Prognostic Model for Multiple Myeloma: Multi-Omics and Machine Learning Insights by Xie L, Gao M, Tan S, Zhou Y, Liu J, Wang L, Li X

“…Results indicated that low-risk patients are more likely to benefit from immunotherapy, while a high MGPS score reflects cellular functional impairment.Conclusion: Our findings reveal a complex interplay between immune cells and MM. …”
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Paper and cardboard as sustainable materials by Jerzy F. Latka

“…With regard to operating energy, materials can be rendered more sustainable by ensuring they have a higher thermal insulation value or greater thermal mass. …”
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Construction and characterization of chimeric FcγR T cells for universal T cell therapy by Juanjuan Zhao, Manling Chen, Xudong Li, Zhaoqi Chen, Wei Li, Rongqun Guo, Min Wang, Zhongxing Jiang, Yongping Song, Jianxiang Wang, Delong Liu

“…Methods Chimeric FcγR (CFR) constructs were generated using three distinct forms of FcγR, namely CD16A, CD32A, and CD64. …”
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The sensor histidine kinase PhcS participates in the regulation of quorum sensing-dependent virulence genes in Ralstonia pseudosolanacearum strain OE1-1 by Wakana Senuma, Masayuki Tsuzuki, Chika Takemura, Yuki Terazawa, Akinori Kiba, Kouhei Ohnishi, Kenji Kai, Yasufumi Hikichi

“…It is thus thought that PhcS participates in not only the 3-OH MAME sensing-independently PhcK-mediated regulation of phcA but also the PhcA activation following 3-OH MAME sensing. Both functions of PhcS are significantly influenced by the autophosphorylation of H230-PhcS.IMPORTANCEThe soil-borne Ralstonia solanacearum species complex (RSSC) infects more than 300 plant species in over 50 families, including solanaceous plants, causing the devastating wilt disease that substantially decreases agricultural production worldwide. …”
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Living and living with nature. A need of today's population for the common good by Manuel Gregorio Loza-Murguia

“…Finally, as human race we depend on the environment and the environment depends on us, we both must live in harmony conserving and preserving our house, each one from its place of origin and its relevance, if only we would do it with integrity we would keep our house at least fit to live in, since there is no other place to go, unless we have a ticket to another planet, although it sounds ridiculous, there is nowhere else to go as human species, in order to preserve ourselves, but being more aware of what we do and what house we want to have and leave to our future generations. …”
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The study of promising secure information systems based on signal modeling by V. A. Sizov, D. M. Malinichev, Kh. K. Kuchmezov

“…Mobile network operators, like consumer electronics manufacturers, will be able to offer “security as a service,” with the result that application providers will be able to apply additional levels of security over existing secure cellular network channels when transferring certain types of data. [7] Due to the better spectral density, the proposed signal conditioning method makes it possible to use prototypes of window functions with the best spatial localization properties without violating the orthogonality condition of the signal bases, and accordingly does not require the use of cyclic prefixes when generating the OFDM signal.…”
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Smartphone-based Monitoring and cognition Modification Against Recurrence of Depression (SMARD): An RCT of Memory Bias Modification Training vs. Cognitive Control Training vs. Atte... by N. Ikani, A. Tyborowska, N. Kohn, N. Günther, G J. Siegle, A. H. Schene, J. A. S. Vorstman, C. J. Harmer, M. J. Kas, J. N. Vrijsen, H. G. Ruhé

“…Abstract Background Major Depressive Disorder (MDD) has a 50–80% recurrence rate highlighting the urgent need for more efficient recurrence prevention programs. Currently, recurrences are often identified too late, while existing preventive strategies may not sufficiently address ethio-patho-physiological mechanisms for recurrence. …”
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Surface hardening of ductile austenitic lightweight steel through powder bed fusion 3D printing by Joonoh Moon, Hyun-Uk Hong, Hyungkwon Park, Hyo-Haeng Jo, Seong-Jun Park, Chansun Shin, Heung Nam Han, Myoung-Gyu Lee, Jae Suk Jeong, Bong Ho Lee, Chang-Hoon Lee

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In Silico and In Vitro Studies of the Approved Antibiotic Ceftaroline Fosamil and Its Metabolites as Inhibitors of SARS-CoV-2 Replication by Cássia Delgado, Pablo Andrei Nogara, Milene Dias Miranda, Alice Santos Rosa, Vivian Neuza Santos Ferreira, Luisa Tozatto Batista, Thamara Kelcya Fonseca Oliveira, Folorunsho Bright Omage, Flávia Motta, Izabela Marques Bastos, Laura Orian, João Batista Teixeira Rocha

“…The 1,2,4-thiadiazole functional group is an inhibitor of cysteine proteases, such as papain and cathepsins. …”
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Caged-hypocrellin mediated photodynamic therapy induces chromatin remodeling and disrupts mitochondrial energy metabolism in multidrug-resistant Candida auris by Xinyao Liu, Yiran Tao, Linwan Zhang, Yuzhou Liu, Dongmei Shi, Jiao Wang, Peng Xue, Bin Xu, Wenjie Fang, Yuping Ran

“…Antimicrobial photodynamic therapy (aPDT), which generates reactive oxygen species (ROS) through light-activated photosensitizers, has shown promise against C. auris; however, its molecular mechanism remains unclear. …”
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CAR-T cells and TRUCKs that recognize an EBNA-3C-derived epitope presented on HLA-B*35 control Epstein-Barr virus-associated lymphoproliferation by Michael Hudecek, Anna Christina Dragon, Katharina Zimmermann, Thomas Nerreter, Deborah Sandfort, Julia Lahrberg, Stephan Klöß, Christina Kloth, Caroline Mangare, Agnes Bonifacius, Sabine Tischer-Zimmermann, Rainer Blasczyk, Britta Maecker-Kolhoff, Barbara Uchanska-Ziegler, Hinrich Abken, Axel Schambach, Britta Eiz-Vesper

“…Functionality of the engineered cells was assessed in co-cultures with EBNA-3C-peptide-loaded, HLA-B*35-expressing K562 cells and EBV-infected B cells as PTLD model. …”
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ADI-270: an armored allogeneic gamma delta T cell therapy designed to target CD70-expressing solid and hematologic malignancies by Jie Zhang, Jyothi Sethuraman, Marissa Herrman, Pranav Murthy, Ramandeep Kaur, Jonathan T S Wong, Kevin P Nishimoto, Smitha R Y Gundurao, Arun Bhat, Blake T Aftab, Shon Green, Aruna Azameera, Morgan Smith-Boeck, Michael Salum, Yvan Chanthery, Melinda Au, Amy Doan, Christopher J Rold, Swapna Panuganti, Helen Budworth, Gauri Lamture, Alexander G Teague, Yogendra Verma, Elizabeth B Speltz, Katherine M Wang, Dishant Bhatwala, Ana Giner-Rubio, Pavan Puligujja, Aya Jakobovits

“…Homing barriers, immunosuppressive factors, and target antigen heterogeneity can impair CAR-T cell functional activity within the TME. Alternative strategies have contemplated incorporating the use of gamma delta (γδ) T cells as a CAR-T cell approach to potentially overcome these limitations. γδ T cells possess both innate and adaptive immunity to facilitate broad tumor recognition, and their natural propensity for tissue tropism may allow for more effective tumor infiltration. …”
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