Development and Validation of Models to Estimate the Incident Risk of Cognitive Impairment and Atherosclerotic Cardiovascular Disease in Older Adults by Michael G. Nanna, Daniel Wojdyla, Eric D. Peterson, Ann Marie Navar, Jeff D. Williamson, Lisandro D. Colantonio, Stephen Y. Wang, Yasser Jamil, Alain G. Bertoni, Musarrat Nahid, Abdulla A. Damluji, Parag Goyal, Sarwat I. Chaudhry, Thomas M. Gill, Karen P. Alexander

“…Variable selection, including functional status, was based on the least absolute shrinkage and selection operator method. …”
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Longitudinal changes in optical coherence tomography angiography before and after surgery for acute primary angle closure glaucoma by Ke Yin, Haomin Chen, Yanxin Zhang, Xinyi Li, Ruihong Su, Yi Yue, Yingjuan Lv, Lijie Dong, Yi Shi, Aihua Liu

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Integrating genome and transcriptome analysis to decipher balanced structural variants in unsolved cases of neurodevelopmental disorders by Simona Mellone, Alice Spano, Denise Vurchio, Giulia Borgonovi, Giulia Borgonovi, Alessandro Ugonotti, Giulia Paglino, Alba Bianco, Sara Ronzani, Maurizio Sciancalepore, Flavia Prodam, Flavia Prodam, Amanda Papa, Maurizio Viri, Umberto Dianzani, Umberto Dianzani, Mara Giordano, Mara Giordano

“…Transcriptomic analysis demonstrated the monoallelic and significantly reduced expression of EP300.ConclusionThese findings highlight the crucial role of WGS in identifying disease-associated BCAs and underscore the complementary value of RNA-Seq in assessing their functional consequences. This integrated approach enhanced diagnostic accuracy and clinical management, paving the way for more comprehensive and personalized care in these two patients.…”
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Gut microbiota alterations are linked to COVID-19 severity in North African and European populations by Marius Bredon, Pierre Hausfater, Loubna Khalki, Youssef Tijani, Amine Cheikh, Loic Brot, Laura Creusot, Nathalie Rolhion, Francois Trottein, Gérard Lambeau, Sophie Georgin-Lavialle, Alexandre Bleibtreu, Jean-Luc Baudel, Antoine Lefèvre, Patrick Emond, Florence Tubach, Noémie Simon-Tillaux, Tabassome Simon, Guy Gorochov, Younes Zaid, Harry Sokol

“…Functional pathways, including L-Tryptophan biosynthesis, were disrupted, particularly in patients with severe disease. …”
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Identification of microbial exopolymer producers in sandy and muddy intertidal sediments by compound-specific isotope analysis by Hubas, Cédric, Gaubert-Boussarie, Julie, D’Hondt, An-Sofie, Jesus, Bruno, Lamy, Dominique, Meleder, Vona, Prins, Antoine, Rosa, Philippe, Stock, Willem, Sabbe, Koen

“…Our study reveals that EPS sources are more diverse in sandy sediments than in muddy sediments. …”
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T cell-specific non-viral DNA delivery and in vivo CAR-T generation using targeted lipid nanoparticles by Jacek Lubelski, Eline van Diest, Ator Ashoti, Christina Zotou, Hanneke Rijssemus, Raymond Schiffelers, Zhiyong Lei, Maurits Geerlings, Jaime Fernández Bimbo, Daniel E Murphy, Martijn J W Evers, Suneel A Narayanavari, Diana Pereira Vaz, Nadine Saber, Peter Mayrhofer

“…NCtx represents a novel non-viral gene therapy vector for in vivo CAR-T therapy, offering a scalable and potentially more accessible alternative to traditional approaches in CAR-T cell generation.…”
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Effectiveness and Safety of Endovascular Treatment in Large Vessel Occlusion Stroke with an NIHSS Score of ≤5 Exhibiting Predominant Cortical Signs by Chulho Kim, Seung Joon Oh, Jae Jun Lee, Jong-Hee Sohn, Joo Hye Sung, Yerim Kim, Minwoo Lee, Mi Sun Oh, Kyung-Ho Yu, Hee Jung Mo, Sang-Hwa Lee

“…<b>Conclusions</b>: EVT may significantly improve the functional outcomes in patients with mild LVO stroke who present with cortical signs, despite low NIHSS scores. …”
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Evaluation of bronchial hyperresponsiveness in asthmatic paediatric patients using mannitol challenge test – Impacts of body mass index by Sharon S. Y. Leung, Helen S. L. Tsang, Jasmine Chan, Oliver Y. H. Kui, Ping Zeng, Yin Ting Cheung, James-Wesley Cheng, Kate C. C. Chan, Michelle Yu, Patricia Tang, John D. Brannan, Jenny K. W. Lam, Hak-Kim Chan, Albert M. Li

“…Background Increasing epidemiological studies reported that overweight/obese asthma patients had more frequent and severe symptoms and exacerbations, indicating their asthma management may not be sufficient. …”
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Epigenetic Drift Is Involved in the Efficacy of HBV Vaccination by Francesca Ferraresi, Simona Anticoli, Stefano Salvioli, Chiara Pirazzini, Luciano Calzari, Davide Gentilini, Christian Albano, Reparata Rosa Di Prinzio, Salvatore Zaffina, Rita Carsetti, Paolo Garagnani, Anna Ruggieri, Katarzyna Malgorzata Kwiatkowska

“…Vaccines allow us to save more than 2.5 million lives each year; however, up to 10% of vaccinated individuals may not develop sufficient protective antibody levels. …”
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DNA methylation variations of DNA damage response in glioblastoma: NSUN5 modulates tumor-intrinsic cytosolic DNA-sensing and microglial behavior by An-an Yin, Yan Yao, Yi-feng Liu, Yu-sha Ji, Ya-long He, Tian-chi Ma, Wen-heng Guo, Amandine Etcheverry, Marc Aubry, Jean Mosser, Wei Lin, Yuan-ming Wu, Kun Chen

“…This may partially account for the functional discrepancy of NSUN5 observed between experimental and clinical contexts. …”
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Immunological landscape of children with Mycoplasma pneumoniae pneumonia in the post-COVID-19 era reveals distinctive severity indicators by Ran Jia, Haiyan Guo, Aizhen Lu, Caiyan Zhang, Yuanyuan Qi, Dingmei Wang, Wen He, Qing Wang, Zimei Cheng, Yajing Gao, Guoping Lu, Libo Wang, Xiaowen Zhai, Jin Xu, Xiaobo Zhang, Yi Wang, Yufeng Zhou

“…Notably, both T helper type 1 and activated regulatory T cells were more abundant in MPP cases, particularly in S-MPP, whereas CD8 + T cells displayed an exhaustion phenotype. …”
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FlyPhoneDB2: A computational framework for analyzing cell-cell communication in Drosophila scRNA-seq data integrating AlphaFold-multimer predictions by Mujeeb Qadiri, Ying Liu, Ah-Ram Kim, Myeonghoon Han, Eric Zhou, Austin Veal, Tzu-Chiao Lu, Hongjie Li, Yanhui Hu, Norbert Perrimon

“…In addition, the algorithm has been optimized for improved performance and more effective noise filtering. New functionalities have also been introduced, such as the addition of downstream reporter genes to evaluate pathway activity, multi-sample CCC comparison, and enhanced visualizations summarizing communication at a network level.We demonstrate the utility of FlyPhoneDB2 by analyzing whole-body single-nuclei RNA-seq datasets from flies with gut tumors induced by the Yorkie oncogene. …”
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Targeted temperature management at 33 versus 36 degrees after out-of-hospital cardiac arrest: A follow-up study by Robert Doerning, Kyle R. Danielson, Jane Hall, Catherine R. Counts, Michael R. Sayre, Sarah Wahlster, James A. Town, Sue Scruggs, David J. Carlbom, Nicholas J. Johnson

“…Aim: Targeted temperature management (TTM) is commonly used in the setting of out-of-hospital cardiac arrest (OHCA) to improve survival and functional outcomes. However, real-world evidence of effects and optimal temperature are limited. …”
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Integrative spatial and single-cell transcriptomics elucidate programmed cell death-driven tumor microenvironment dynamics in hepatocellular carcinoma by Kai Lei, Kai Lei, Yutong Zhao, Shumin Li, Jiawei Liu, Wenhao Chen, Caihong Zhou, Yi Zhang, Jinmei Tan, Jian Wu, Qi Zhou, Qi Zhou, Jiehui Tan

“…T cells in high-PCD patients were more likely to be exhausted, with elevated expression of exhaustion markers. …”
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Cell-Free DNA (cfDNA) Regulates Metabolic Remodeling in the ES-2 Ovarian Carcinoma Cell Line, Influencing Cell Proliferation, Quiescence, and Chemoresistance in a Cell-of-Origin-Sp... by Isabel Lemos, Catarina Freitas-Dias, Ana Hipólito, José Ramalho, Fabrizio Carteni, Luís G. Gonçalves, Stefano Mazzoleni, Jacinta Serpa

“…We observed instant reactions of adopting the metabolic profile that brings back the cell functioning of more favorable culture conditions supporting proliferation and resembling the cell of origin of the cfDNA variant, as observed in unselected ES-2 cells. …”
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Relieving acute pain (RAP) study: a proof-of-concept protocol for a randomised, double-blind, placebo-controlled trial by Thomas Scalea, Luana Colloca, Se Eun Lee, Meghan Nichole Luhowy, Nathaniel Haycock, Chika Okusogu, Soojin Yim, Nandini Raghuraman, Robert Goodfellow, Robert Scott Murray, Patricia Casper, Myounghee Lee, Yvette Fouche, Sarah Murthi

“…Primary and secondary outcomes include feasibility and acceptability of the study. Pain intensity, functional pain, emotional distress, rates of rescue therapy requests and patient-initiated medication denials will be collected.Ethics and dissemination All activities associated with this protocol are conducted in full compliance with the Institutional Review Board policies and federal regulations. …”
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Prevalence of Extended-Spectrum β-Lactamase-Producing <i>Escherichia coli</i>, <i>Klebsiella pneumoniae</i> and <i>Enterobacter cloacae</i> in Wastewater Effluent in Blantyre, Mala... by Edna Ibrahim, Charity Mkwanda, Edward Masoambeta, Luigia Scudeller, Tomislav Kostyanev, Hussein H. Twabi, Yohane K. Diness, Jobiba Chinkhumba, Janelisa Musaya, Rajhab S. Mkakosya, Surbhi Malhotra-Kumar, Chantal M. Morel, Save Kumwenda, Chisomo L. Msefula

“…<b>Results</b>: A total of 288 samples were collected between April 2023 and March 2024, and 97.6% (281/288) yielded one or more presumptive ESBL isolates. Bacterial growth was confirmed as 48.9% <i>Ec</i> (255/522), 33.0% <i>Kp</i> (172/522), and 10.0% <i>Enc</i> (52/522). …”
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Stable water isotopes in soil–plant systems reveals ecohydrological dynamics in a typical flood wetland in the subtropical monsoon region, China by Shiyong Tao, Jun Xia, Junqiang Xia, Xiaoqiang Chen, Wenjun Lv, Haijiao Gui, Jing Xu

“…The temporal isotopic fluctuations in shallow soil water (0∼40 cm) were more pronounced, and frequent abrupt isotopic enrichment of subsurface layers was attributed to the ubiquitous preferential flow. …”
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Non-feature-specific elevated responses and feature-specific backward replay in human brain induced by visual sequence exposure by Tao He, Xizi Gong, Qian Wang, Xinyi Zhu, Yunzhe Liu, Fang Fang

“…It remains unknown whether the elevated responses are feature-specific and, more crucially, how the brain organizes sequence information after exposure. …”
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Carbon and nitrogen cycling in an urban constructed technosol: The artist-led carbon sponge pilot study by Sara Perl Egendorf, Maha Deeb, Brooke Singer, Nkwi Flores, Marisa Prefer, Zhongqi Cheng, Peter Groffman

“…However, CTs must be better understood, particularly in terms of their nutrient dynamics, to optimize their ecological functions. In the Carbon Sponge pilot study, located at the New York Hall of Science in Queens, NY, USA, our objective, through an artist-led collaboration, was to evaluate the effects of different plant communities on CT formation and carbon (C) and nitrogen (N) cycling. …”
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