Accounting for tumor heterogeneity when using CRISPR-Cas9 for cancer progression and drug sensitivity studies. by Jessica F Olive, Yuanbo Qin, Molly J DeCristo, Tyler Laszewski, Frances Greathouse, Sandra S McAllister

“…We describe a novel CRISPR/Cas9 protocol that incorporates a single-cell cloning step prior to gene editing, allowing for the generation of appropriately matched, functionally equivalent control and edited cell lines. …”
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Superiorly-based, preauricular tunneled insular flap for reconstruction of auricular defects by Délcio Aparecido Durso, Willian Segalin, Paolla Ribeiro Gonçalves, Marcella Ferreira Carvalho, Sérgio Domingo Bocardo, Flávia Freire Dantas

“…We report the case of a patient who presented with tumor lesions on more than one occasion in the anterior left ear and required surgical intervention to correct the defect generated by excision. …”
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Is the Hip Keeping the Ankle Out of Work after Total Ankle Arthroplasty? by Frank DiLiberto PT, PhD, Maxine Olson MS, Finola Summerville, Stacey Meardon PhD, PT, Anand Vora MD

“…Of note, postoperative ankle energy production (i.e. power, work) during gait is also deficient and related to lower gait speed and physical function scores. These findings raise questions regarding if people after TAA have the capacity to generate greater ankle energy or rely on proximal joint (e.g. hip) energy during walking and more challenging daily functional tasks. …”
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Proteomic profiling of small extracellular vesicles from bovine nucleus pulposus cells. by Ankita Samanta, Mi-Jeong Yoo, Jin Koh, Sina Charlotte Lufkin, Thomas Lufkin, Petra Kraus

“…We investigated autologous and mesenchymal stem cell (MSC) small EVs for their potential to contribute to cell and tissue homeostasis in the NP niche via mass spectrometric proteome and functional enrichment analysis using adult and fetal donors. …”
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In Silico identification and annotation of non-coding RNAs by RNA-seq and De Novo assembly of the transcriptome of Tomato Fruits. by Daria Scarano, Rosa Rao, Giandomenico Corrado

“…Moreover, our work demonstrates the usefulness of strand specific RNA-seq approach for the transcriptome-based genome annotation and provides a resource valuable for further functional studies.…”
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Stroke survivors’ perceptions of living with disability in urban Ghana- a qualitative study by Petter Persson, Anna Hughton, Sibylle Herzig van Wees

“…Conclusion Stroke survivors with disabilities perceive themselves as non-functional members of society, which limits their options for a normal life and undermines their well-being. …”
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Sucla2 Knock‐Out in Skeletal Muscle Yields Mouse Model of Mitochondrial Myopathy With Muscle Type–Specific Phenotypes by Makayla S. Lancaster, Paul Hafen, Andrew S. Law, Catalina Matias, Timothy Meyer, Kathryn Fischer, Marcus Miller, Chunhai Hao, Patrick Gillespie, David McKinzie, Jeffrey J. Brault, Brett H. Graham

“…Physiological and morphological analyses of hindlimb muscles showed remarkable differences in ex vivo function and cellular consequences between the EDL and SOL muscles, with SOL muscles significantly more impacted by Sucla2 inactivation. …”
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Neural Mechanisms of Circadian Regulation of Natural and Drug Reward by Lauren M. DePoy, Colleen A. McClung, Ryan W. Logan

“…Circadian rhythms are endogenously generated near 24-hour variations of physiological and behavioral functions. …”
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Brain Signals of Face Processing as Revealed by Event-Related Potentials by Ela I. Olivares, Jaime Iglesias, Cristina Saavedra, Nelson J. Trujillo-Barreto, Mitchell Valdés-Sosa

“…In turn, the relatively late and probably more rostrally generated N250r and N400-like responses might respectively indicate processes of access and retrieval of face-related information, which is stored in long-term memory (LTM). …”
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Correcting Reflux Laparoscopically by Eric C Poulin, Christopher M Schlachta, Joseph Mamazza

“…Most believe that the trend towards procedures that minimize or eliminate the trauma of surgery while adhering to accepted surgical principles is irreversible. The functional results of laparoscopic antireflux surgery in the seven years since its inception have been virtually identical to the success curves generated with open fundoplication in past years. …”
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The gene signature linked to lactate metabolism predicts the prognosis and correlates with the immune status of head and neck squamous cell carcinoma by Jian Xiao, Wei Li, Guolin Tan, Ru Gao

“…Additionally, eight critical hub genes were identified, revealing significant differences in gene expression between risk score groups. Functional analyses demonstrated that the low-risk group exhibited a more favorable prognosis and enhanced immune characteristics. …”
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A New Extention of the Odd Inverse Weibull-G Family of Distributions: Bayesian and Non-Bayesian Estimation with Engineering Applications by Yassmen Y. Abdelall, Amal Soliman Hassan, Ehab M. Almetwally

“…In this work, we propose a novel generator called the "extended odd inverse Weibull-generator" to obtain better distribution flexibility. …”
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Influence of land use intensity on ecological corridors and wildlife crossings’ effectiveness: comparison of 2 pilot areas in Austria by Mořic Jurečka, Richard Andrášik, Petr Čermák, Florian Danzinger, Christoph Plutzar, Roland Grillmayer, Tomáš Mikita, Tomáš Bartonička

“…Human development and induced activities significantly affect the natural functioning of ecosystems and hence landscape connectivity. …”
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Impact of temperature on tyre hardness by David Jelínek, Marek Semela

“…From the results it is clear that all samples show a polynomial function with a 2nd order polynomial for the temperature range −10 °C to 65 °C. …”
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Analyse d'une formation plurilingue à distance : actions et interactions by Jean-Jacques Quintin, Monica Masperi

“…Galanet" learning schemes assemble large groups of students (from one to two hundred in general) who are geographically located in different universities and committed to completing common tasks the final objective of which is to create a quadrilingual press file publication online.The present study will be developed along two complementary axes:1. a quantitative analysis of the messages posted on the forum page of the platform for each session.2. a qualitative analysis of sample interventions generated in one of these sessions.On the one hand, the quantitative approach will allow for the highlighting of certain salient features of the e-learning experience as a whole, including how sessions function and their impact on both students and tutors.On the other hand, the qualitative approach will allow to examine and understand more fully the discursive practices elicited by the learning scenario and the plurilinguistic dimension of the forum exchanges.…”
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Optimization design on process parameters of copper wire bonding for power modules by HU Biao, CHENG Lanxian, LI Zhenling, DAI Xiaoping

“…Secondly, a prediction model of copper wire bonding performance was constructed using the BP neural network, and the optimal values of process parameters were generated by solving the BP neural network fitness function with GA. …”
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Optimization and Analysis of Double-circular-arc Harmonic Gear based on Genetic Algorithm by Zhonglong Luo, Xiaochuan Peng

“…Through analyze the single parameter sensitivity of the optimized parameters， it is found that the objective function has a low sensitivity to the arc radius of convex and concave tooth， and the tooth thickness ratio. …”
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How a lack of haptic feedback affects eye-hand coordination and embodiment in virtual reality by Ewen Lavoie, Jacqueline S. Hebert, Craig S. Chapman

“…Returning haptic feedback in VR generated eye-hand coordination more similar to real-world interactions. …”
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Method to Correct the Velocity Variation Information of an Automatic Crash Notification System in Vehicle-to-Rigid Barrier Frontal Collisions by Ying Lu, Xiaojie Ji, Yu Shu

“…Third, an automatic crash notification algorithm involving a velocity variation correction function (VVCF) was proposed based on the correlation model. …”
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China’s Fiscal and Monetary Policies During the Global Financial Crisis and the Covid-19 Pandemic: Lessons and Policy Implications by Trương Quang Thông

“…The study generated the following major findings: First, the transition from loosening monetary policy to tightening monetary policy at the time of 2008 GFC is one notable approach and a valuable lesson learned; Second, prioritization of lifesaving and enhancing economic resiliency should be at the core in addressing the economic challenges, particularly during the Covid-19 epidemic; and Third, the importance of the central bank to function as a transmission belt for government to flood the country with liquidity through commercial bank lending.…”
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