In vivo CRISPR screening identifies POU3F3 as a novel regulator of ferroptosis resistance in hepatocellular carcinoma via retinoic acid signaling by Yu Tian, Xin Bao, Shan Lei, Youcai Huang, Xiaoling Wang, Yanyang Tu, Qinglian He, Feixiang Zhang, Haicheng Xu, Milad Ashrafizadeh, Gautam Sethi, Furong Wang, Zhirui Zeng

“…The effects and underlying molecular mechanisms of these resistance factors were investigated in HCC cells using ferroptosis detection assays, xenograft tumor models, chromatin immunoprecipitation (ChIP), and dual-luciferase reporter assays. …”
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SARS‐CoV‐2 Is Linked to Brain Volume Loss in Multiple Sclerosis by Tomas Uher, Dominika Stastna, Ingrid Menkyova, Vaclav Capek, Jiri Lindner, Petra Nytrova, Jan Krasensky, Eliza Varju, Miguel D'haeseleer, Eva Kubala Havrdova, Dana Horakova, Manuela Vaneckova, Niels Bergsland

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Cerebrospinal fluid proteome profiling across the Alzheimer’s disease continuum: a step towards solving the equation for ‘X’ by Sophia Weiner, Mathias Sauer, Laia Montoliu-Gaya, Andrea L. Benedet, Nicholas J. Ashton, Fernando Gonzalez-Ortiz, Joel Simrén, Nesrine Rahmouni, Cecile Tissot, Joseph Therriault, Stijn Servaes, Jenna Stevenson, Ville Leinonen, Tuomas Rauramaa, Mikko Hiltunen, Pedro Rosa-Neto, Kaj Blennow, Henrik Zetterberg, Johan Gobom

“…Weighted protein co-expression network analysis identified clusters of proteins robustly correlating in all three cohorts which sequentially changed with AD progression. …”
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DOT1L protects against podocyte injury in diabetic kidney disease through phospholipase C-like 1 by Yepeng Hu, Shu Ye, Jing Kong, Qiao Zhou, Zhe Wang, Yikai Zhang, Han Yan, Yaqiong Wang, Tiekun Li, Yi Xie, Bingbing Chen, Yiming Zhao, Tianyue Zhang, Xianan Zheng, Junjia Niu, Bibi Hu, Shengyao Wang, Zhida Chen, Chao Zheng

“…Methods The expression of DOT1L was confirmed by Western blotting in MPC-5 cells and cortex of kidney from db/db mice, as well as immunofluorescence staining in human renal biopsy samples. …”
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Lifestyle factors and urine levels of organophosphorus flame retardants in endometrial cancer: insights from a case-control study by Yu-Che Ou, Fu-Jen Cheng, Wan-Ting Huang, Wen-Chin Lee, Hung-Chun Fu, Chen-Hsuan Wu, Ying-Yi Chen, Kuo-Chung Lan, Xuan-Ping Liou, Hao Lin, Chia-Te Kung

“…Significantly higher levels of OPFRs in EC patients were identified, and logistic regression models were used to determine whether elevated OPFRs were associated with EC and to explore whether any lifestyle behaviors contributed to the increased OPFR levels. …”
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Patterns of Factors in the National Institute on Aging Health Disparities Research Framework Domains and Mild Cognitive Impairment Risk by Maria Vassilaki, MD, PhD, Jeremiah A. Aakre, MPH, Timothy G. Lesnick, MS, Walter K. Kremers, PhD, Jonathan Graff-Radford, MD, David S. Knopman, MD, Thomas H. Mosley, PhD, B. Gwen Windham, MD, MHS, Michael E. Griswold, PhD, Yonas E. Geda, MD, MSc, Val J. Lowe, MD, Clifford R. Jack, Jr., MD, Ronald C. Petersen, MD, PhD, Prashanthi Vemuri, PhD

“…Using a cohort study design, the resulting composite scores were considered as covariates for incident mild cognitive impairment analysis using Cox proportional hazards models. Results: Three principal components explained 40.30% of the variance and were differentially associated with mild cognitive impairment risk. …”
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Association of hyperactivity–impulsivity and inattention symptom profiles with suicide attempt: an 18-year population-based cohort study by Samuele Cortese, Cédric Galéra, Marie-Claude Geoffroy, Gustavo Turecki, Richard E Tremblay, Ayla Inja, Sylvana M Côté, Michel Spodenkiewicz, Isabelle Ouellet-Morin, Michel Boivin, Frank Vitaro, Mara Brendgen, Ginette Dionne, Johanne Renaud, Massimilano Orri

“…Suicide attempt in adolescence and young adulthood (by age 23) was self-reported. Multitrajectory modelling was used to identify profiles of ADHD symptoms, and regression analysis was used to test their association with suicide attempt, adjusting for childhood socioeconomic and clinical characteristics.Findings We identified four ADHD symptom profiles with distinct associations with suicide attempt for males and females. …”
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TGFB1/CXCL5 axis regulation by LCN2 overexpression: a promising strategy to inhibit colorectal cancer metastasis and enhance prognosis by Xiaotian Song, Xiaotian Song, Shuai Xu, Shuai Xu, Dan Song, Juan Wang, Bin Bai, Yanxin An, Bin Yang, Shiqi Wang, Qingchuan Zhao, Pengfei Yu

“…We evaluated the migratory and invasive capabilities of CRC cells influenced by LCN2 using in vitro transwell assays and in vivo lung metastatic models. RNA sequencing and proteome analysis were employed to identify potential downstream targets of LCN2. …”
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Defects in the necroptosis machinery are a cancer resistance mechanism to checkpoint inhibitor immunotherapy by Katja Steiger, Peter May, Florian Bassermann, Anna Sax, Stefan Enssle, Nardine Soliman, Tatiana Nedelko, Giada Mandracci, Fabian Stögbauer, Laura Joachim, Christof Winter, Nadia El Khawanky, Hendrik Poeck, Simon Heidegger

“…However, it is unclear to which extent functional necroptosis in tumor cells contributes to ICI immunotherapy.Methods With genetically engineered tumor cell lines that lack specific components of the necroptosis machinery (mixed lineage kinase domain-like pseudokinase (MLKL), receptor interacting protein kinase 3 (RIPK3)), we addressed the importance of necroptotic tumor cell death for the efficacy of ICI immunotherapy in murine models. …”
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Beneficial effects of reduced soft tissue vibrations with compression garments on delayed neuromuscular impairments induced by an exhaustive downhill run by Robin Gassier, Loïc Espeit, Antoine Ravel, Pierre Beaudou, Robin Trama, Pascal Edouard, Pascal Edouard, Arsène Thouze, Léonard Féasson, Léonard Féasson, Frédérique Hintzy, Jérémy Rossi, Christophe Hautier

“…However, an in-depth analysis of changes in STV has never been proposed, and previous protocols did not overcome the repeated bout effect. …”
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Gender and age differences in weekend eating habits: associations with fat mass percentage in a cross-sectional study by Stefania Gorini, Stefania Gorini, Elisabetta Camajani, Elisabetta Camajani, Alessandra Feraco, Alessandra Feraco, Andrea Armani, Andrea Armani, Chiara Quattrini, Maria Grazia Tarsitano, Edda Cava, Laura Di Renzo, Massimiliano Caprio, Massimiliano Caprio, Antonino De Lorenzo, Mauro Lombardo

“…This study investigates gender and age differences in weekend eating behaviors and their association with fat mass percentage (FM%).MethodsA cross-sectional study was conducted on 2,596 participants at an obesity center in Italy. …”
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Adventitial fibroblasts direct smooth muscle cell-state transition in pulmonary vascular disease by Slaven Crnkovic, Helene Thekkekara Puthenparampil, Shirin Mulch, Valentina Biasin, Nemanja Radic, Jochen Wilhelm, Marek Bartkuhn, Ehsan Bonyadi Rad, Alicja Wawrzen, Ingrid Matzer, Ankita Mitra, Ryan D Leib, Bence Miklos Nagy, Anita Sahu-Osen, Francesco Valzano, Natalie Bordag, Matthias Evermann, Konrad Hoetzenecker, Andrea Olschewski, Senka Ljubojevic-Holzer, Malgorzata Wygrecka, Kurt Stenmark, Leigh M Marsh, Vinicio de Jesus Perez, Grazyna Kwapiszewska

“…Bidirectional cellular crosstalk was examined using direct and indirect co-culture models, and phenotypic responses were assessed via transcriptome analysis. …”
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Prognostic impact of gait speed, muscle strength and muscle mass in chronic heart failure—A prospective cohort study by Vladimir Cejka, Hermann Riepl, Nora Schwegel, Ewald Kolesnik, David Zach, Viktoria Santner, Viktoria Höller, Natascha Schweighofer, Barbara Obermayer‐Pietsch, Thomas Pieber, Caroline Morbach, Stefan Frantz, Andreas Zirlik, Dirk vonLewinski, Stefan Störk, Florian Posch, Klemens Ablasser, Nicolas Verheyen

“…Fine and Gray regression models were calculated, treating non‐cardiovascular death as the competing event. …”
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Identification and preclinical efficacy evaluation of two lytic bacteriophages targeting highly virulent and multidrug-resistant Klebsiella pneumoniae by Ai Guo, Dianbao Zuo, Li Shi, Ming Guo, Jinquan Li, Caili Li, Puqing Wang, Xiaodong Sun, Ming Sang

“…More importantly, we established the murine pneumonia infection models through nasal instillation, assessed the therapeutic potential of the phage in vivo by observing pathological morphology via HE staining, detecting pro-inflammatory cytokine levels via qPCR and ELISA, and monitoring bacterial load changes in lung tissue through PCR analysis. …”
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Central Neck Dissection in Papillary Thyroid Carcinoma: Benefits and Doubts in the Era of Thyroid Lobectomy by Jacopo Zocchi, Gioacchino Giugliano, Chiara Mossinelli, Cecilia Mariani, Giacomo Pietrobon, Francesco Bandi, Stefano Malpede, Enrica Grosso, Marco Federico Manzoni, Elvio De Fiori, Giovanni Mauri, Manila Rubino, Marta Tagliabue, Mohssen Ansarin

“…Survivals were analyzed using the Kaplan–Meier estimator and Cox proportional hazard models. Results: A total of 657 patients were included in this study with a median follow-up of 78 months (48–114 months). …”
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Outcomes and cost-effectiveness of an integrated holistic care package on persons affected by podoconiosis, lymphatic filariasis and leprosy and community members in north-western... by Oumer Ali, Awoke Mihretu, Natalia Hounsome, Vasso Anagnostopoulou, Stephen A. Bremner, Mersha Kinfe, Asrat Mengiste, Maya Semrau, Abebaw Fekadu, Gail Davey

“…Mixed effects linear regression models were used to estimate change in outcomes between baseline and 6 months after initiation of the care package. …”
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Pancreatic, nutritional and clinical outcomes in children 0–5 years with cystic fibrosis during the first 2 years of CFTR modulator therapy (PaNC): a multicentre prospective observ... by Tamarah Katz, Sophie King, Gillian M Nixon, Caitlin Miles, Jodi Grunert, Kristyn Ford, Carla Hall, Charlotte Hawthorn, Denise Wong See, Mylie McMahon, Hannah Vass, Sarah Watkins, Gemma Zanardo, Isabella Brookes, Siobhan Handley, Talia Woodward, Jasmin Wademan, Bella Cameron, Zoe Davidson

“…There is a lack of robust, real-world longitudinal data on CFTR modulator therapy in infants and young children where extrapulmonary outcomes such as growth, micronutrient status and pancreatic function are the key focus.Methods and analysis Pancreatic, nutritional and clinical outcomes in children 0–5 years with CF during the first 2 years of CFTR modulator therapy (PaNC) is a prospective cohort study involving all eight tertiary paediatric CF centres in Australia. …”
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Integrated machine learning reveals the role of tryptophan metabolism in clear cell renal cell carcinoma and its association with patient prognosis by Fan Li, Haiyi Hu, Liyang Li, Lifeng Ding, Zeyi Lu, Xudong Mao, Ruyue Wang, Wenqin Luo, Yudong Lin, Yang Li, Xianjiong Chen, Ziwei Zhu, Yi Lu, Chenghao Zhou, Mingchao Wang, Liqun Xia, Gonghui Li, Lei Gao

“…Methods The influence of tryptophan metabolism on tumor cells was explored using single-cell RNA sequencing data. …”
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Typology of out-of-home eaters: a description of sociodemographic, lifestyle, nutritional and environmental characteristics in the NutriNet-Santé cohort by Emma Meyer, Benjamin Allès, Justine Berlivet, Sandrine Péneau, Alice Bellicha, Mathilde Touvier, Brigitte Langevin, Philippe Pointereau, Denis Lairon, Serge Hercberg, Emmanuelle Kesse-Guyot, Julia Baudry

“…This study aimed to identify a typology of OOH consumers by frequency and type of meal consumed OOH and their associated sociodemographic, lifestyle, nutritional, and environmental characteristics. Methods Based on a sub-sample of adults of the French NutriNet-Santé cohort who completed a food frequency questionnaire and a questionnaire on OOH consumption habits in 2014 (n = 29,140, mean age: 53.6 (SD = 14.0) years, 74.3% women), we conducted a Multiple Factor Analysis followed by a clustering procedure. …”
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Association between sleep quality and mild cognitive impairment in Chinese patients with type 2 diabetes mellitus: a cross-sectional study by Rehanguli Maimaitituerxun, Hengxue Wang, Wenhang Chen, Jingsha Xiang, Yu Xie, Fang Xiao, Xin Yin Wu, Letao Chen, Jianzhou Yang, Aizhong Liu, Songning Ding, Wenjie Dai

“…Pearson’s correlation analysis showed that the total PSQI score was negatively associated with the MMSE score (r=-0.27, P < 0.05). …”
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