Digital PCR outperforms quantitative real-time PCR for the detection and quantification of major periodontal pathobionts by Haris Munjaković, Katja Povšič, Mario Poljak, Katja Seme, Rok Gašperšič, Lucijan Skubic

“…Background This study comparatively evaluated the analytical and diagnostic performance of a multiplex digital polymerase-chain reaction (dPCR) assay and a quantitative real-time PCR (qPCR) for the simultaneous detection and quantification of periodontal pathobionts: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Fusobacterium nucleatum.Materials and Methods Subgingival plaque samples from 20 periodontitis patients and 20 periodontally healthy controls were analyzed. …”
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DEVELOPMENT AND VALIDATION OF A NEW RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION AND QUANTIFICATION OF SOFOSBUVIR AND DACLATASVIR by Syed Hassan Murtaza, Ahmed Umer Sohaib Ahmed, Tassawer-e-Meran, Sonam Anees, Najam-ul-Hassan Khan, Kishwar Sultana

“…This method is successfully applied for raw materials as well as in combination tablets. This method may also be applied for the quantification of sofosbuvir and daclatasvir in blood samples of patients taking these two antivirals for hepatitis C treatment. …”
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Diagnostic value of Epstein – Barr virus DNA quantification in blood leukocytes in children with infectious mononucleosis by M. I. Popkova, E. N. Filatova, E. A. Soboleva, D. A. Bryzgalova, E. A. Kulova, N. A. Sakharnov, O. V. Utkin

“…To determine the threshold value of the Epstein-Barr virus (EBV) viral load (VL) in blood leukocytes to improve the laboratory diagnostics of infectious mononucleosis in children.Materials and methods. EBV DNA quantification in blood leukocytes in children aged 1-17 years (n=163) was determined by real-time polymerase chain reaction. …”
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Unveiling hidden contaminants: a systematic quantification and characterization of microplastics in hemodialysis and peritoneal dialysis fluids by Ekrem Kara, Kamil Konur, Yasemen Şentürk Koca, Ülgen Aytan

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Development and Validation of Sibutramine Determination in Drug Products by Capillary Electrophoresis by A. M. Sukhanova, I. B. Perova, K. I. Eller, G. M. Rodionova, S. V. Chernova, V. N. Kuzina

“…To develop and validate a methodology for the determination of sibutramine in drugs by capillary electrophoresis (CE) using an ultraviolet diode array detector.Materials and methods. Quantitative determination of sibutramine in drugs was carried out using the CE method with an ultraviolet diode array detector. …”
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Comparing miR-16 and miR-1228 as an optimal endogenous control for quantification of circulating microRNAs in colorectal cancer patients by Chia-Lin Yang, Fu-Ming Tsai, Chuang-Wei Chen, Kuan-Hong Hsiao, Jia-Hui Chen, Woei-Yau Kao

“…Materials and Methods: The cel-miR-39 was a spiked-in RNA used as an external control and added to plasma samples before RNA extraction. …”
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Quantification and evaluation of strain reduction from small-bubble gas injection in Spallation Neutron Source target vessels by Hao Jiang, Drew E. Winder, David A. McClintock

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Austenite evolution and quantification in aged 250 maraging steel: A comparative analysis of X-ray diffraction methods by Fábio Lima da Silva, Julianna Magalhães Garcia, Alaelson Vieira Gomes, Luiz Paulo Mendonca Brandao

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Quantification of Glucosinolates in Seeds by Solid-State ¹³C-Nuclear Magnetic Resonance (NMR) by Stefania Mazzini, Marco Zuccolo, Angela Bassoli, Claudio Gardana, Gigliola Borgonovo

“…In this study, we evaluated solid-state nuclear magnetic resonance spectroscopy as an alternative method for the identification and quantification of total glucosinolates in the seeds of <i>Sisymbrium officinale</i>, <i>Brassica napus</i>, <i>Sinapis alba</i>, <i>Brassica nigra</i>, and <i>Moringa oleifera</i>. …”
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Wood waste quantification from the furniture industry in Ubá (Brazil) and its reuse prospects in civil construction by Roziani Maria Gomes, Antônio Cleber Gonçalves Tibiriçá, José Maria Franco de Carvalho, Gustavo Henrique Nalon, Leonardo Gonçalves Pedroti, Marcos Oliveira de Paula

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Automatic Quantification Software for Geographic Atrophy Associated with Age-Related Macular Degeneration: A Validation Study by José M. Ruiz-Moreno, Jorge Ruiz-Medrano, Francisco Lugo, Belen Sirvent, Ignacio Flores-Moreno

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Optimization of mammosphere formation assay for quantification of il6-induced stemness in differentiated breast cancer cells by K. V. Nevskaya, L. V. Efimova, P. V. Korda, D. S. Dolgasheva, M. K. Ibragimova, M. E. Menyailo, E. V. Udut, N. V. Litviakov, A. G. Pershina

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A Miniaturized Device Based on Cobalt Oxide Nanoparticles for the Quantification of Uric Acid in Artificial and Human Sweat by Carlos D. Ruiz-Guerrero, Dulce V. Estrada-Osorio, Alejandro Gutiérrez, Fabiola I. Espinosa-Lagunes, Gabriel Luna-Barcenas, Ricardo A. Escalona-Villalpando, Luis G. Arriaga, Janet Ledesma-García

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Wedge splitting test method: quantification of influence of glued marble plates by two-parameter fracture mechanics by S. Seitl, B. Nieto García, I. Merta

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Recurrence Quantification Analysis of Center of Pressure Trajectories for Balance and Fall-Risk Assessment in Young and Older Adults by Emiliano Fernandez-Cervantes, Luis Montesinos, Andres Gonzalez-Nucamendi, Leandro Pecchia

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Development of Fast Analytical Method for the Detection and Quantification of Honey Adulteration Using Vibrational Spectroscopy and Chemometrics Tools by Omar Elhamdaoui, Aimen El Orche, Amine Cheikh, Brahim Mojemmi, Rachid Nejjari, Mustapha Bouatia

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Automated Deep Learning Based Cardiac Quantification in Hypertrophic Cardiomyopathy: A Comparative Study with Manual Segmentation by Shivam Angiras, Deb Kumar Boruah, Pranjal Phukan, Kalyan Sarma, Prince Das, Rajeev Bharadwaj, Harshit Jain, Ajmal Roshan

“…Recently, Deep Learning (DL) algorithms have emerged to automate cardiac quantification, but their performance in complex pathologies such as HCM still requires validation.Purpose: To compare the performance of a fully automated deep learning-based cardiac segmentation software (SW 2) (SuiteHEART) with conventional manual segmentation (SW 1) (syngo.Via) for quantifying crucial cardiac parameters in patients with HCM.Materials and Methods: In this prospective study, 25 consecutive adult patients (mean age 49±12 years) with HCM referred for CMR at our institute were included. …”
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Quantification of solution annealing effects on microstructure and property in a laser powder bed fusion 316H stainless steel by Lin Gao, Srinivas Aditya Mantri, Xuan Zhang

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Uncertainty quantification of thermal barrier coatings lifetime on rotating turbine blades considering chemo-thermo-mechanically coupling failure by Weiliang Yan, Cong Li, Qianqian Zhou, Yuqi Xie, Yu Sun, Li Yang, Yichun Zhou

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Experimental assessment and data-driven hybrid machine learning quantification with parametric optimization of compressive strength of ceramic waste concrete by Mihir Mishra, Md. Habibur Rahman Sobuz, Md. Kawsarul Islam Kabbo, M Jameel, Turki S. Alahmari, Naim Ahmad, Md. Munir Hayet Khan

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