Thermal Inactivation of Salmonella, Escherichia coli, and Enterococcus faecium NRRL B-2354 in Pasta Matrices by Kristi S. Gowans, Bradley J. Taylor, Brad D. Geary, Richard A. Robison, Gene J. Ahlborn

“…The appropriateness of E. faecium NRRL B-2354 as a surrogate in semolina flour and pasta matrices was determined. Inoculated pasta matrices equilibrated to target water activities of 0.85, 0.88, and 0.91 (at 25°C) underwent isothermal inactivation treatments at 65°C, 70°C, and 75°C. …”
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Corynebacteria from the respiratory microbiota modulate inflammatory responses and associate with a reduced pneumococcal burden in the lungs by Caroline Bergenfelz, Phuong Do, Liv Larsson, Hanna Ivarsson, Kasper Malmborn, Anders P. Håkansson

“…The specific role of Corynebacteria during respiratory health and disease is, however, largely uncharacterized.MethodRespiratory tract epithelial cells NCI-H292 and BALB/cByJ mice were inoculated with Corynebacteria (C. propinquum 2018M3 and 2019M4, and C. pseudodiphtheriticum 2019M8 and 2020M12) alone or with subsequent challenge with Streptococcus pneumoniae (pneumococci). …”
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Bioremediation of Polycyclic Aromatic Hydrocarbon contaminated Aqueous-Soil matrix: Effect of co-contamination by CN Owabor, OC Onwuemene, I Enaburekhan

“…The soil was sterilized by heating at 120oC for one hour. 100g of the soil was contaminated with lead, chromium, nickel and mercury (40-200mg/l), 200mg/l of naphthalene and finally inoculated with microbes (Bacillus spp and Aspergillus niger). …”
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RESULTS OF EXPERIMENTAL INFECTION OF TURKEYS WITH A/DUCK/ALTAI/469/14 H5N1 STRAIN OF AVIAN INFLUENZA VIRUS by V. Yu. Sosipatorova, D. A. Altunin, I. A. Chvala, N. S. Mudrak, D. B. Andreychuk

“…The characteristics of the infection process in birds inoculated intranasally at a dose of 5.0 lg EID50/0.5 cm3 are described with an indication of the incubation period and the mean time of death. …”
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Factors affecting heat resilience of drone honey bees (Apis mellifera) and their sperm. by Alison McAfee, Bradley N Metz, Patrick Connor, Keana Du, Christopher W Allen, Luis A Frausto, Mark P Swenson, Kylah S Phillips, Madison Julien, Zoe Rempel, Robert W Currie, Boris Baer, David R Tarpy, Leonard J Foster

“…Further investigating potential causal factors for such variation, we found no association between drone mass and viability of sperm in in vitro sperm heat challenge assays, but virus inoculation (with Israeli acute paralysis virus) exacerbated the negative effect of heat on sperm viability. …”
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Plant Growth Promoting Rhizobacteria (PGPR): Reports on Their Colonization, Beneficial Activities, and Use as Bioinoculant by Dew Biswas, Amit Kumar Chakraborty, Vikas Srivastava, Arunava Mandal

“…Commercial development of the PGPR inoculants with remarkable biostimulating activities and their successive application should be expanded through collaborative association with different sectors after the removal of existing lacunae. …”
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An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture by Mahipal S. Shekhawat, M. Manokari, C. P. Ravindran

“…Nodal explants were sterilized with 0.1% HgCl2 and inoculated on Murashige and Skoog (MS) basal medium. …”
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Isolation, Serotyping, and Molecular Detection of Bovine FMD Virus from Outbreak Cases in Abaʼala District of Afar Region, Ethiopia by Teshager Dubie, Tsedale Amare

“…Out of 27 samples, 18 of them were inoculated on cultured Baby hamster kidney (BHK-21) monolayer cells, and all 27 samples were tested using conventional RT-PCR and sets of specific universal primers. …”
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MODERN TECHNOLOGIES OF WINE PRODUCTION IN THE CONTEXT OF GLOBAL WARMING AND ORGANIC APPROACH IN VINEYARD by N. Kamenieva, O. Verechuk

“…All wine production processes are interconnected, so changes will inevitably affect each of them - from the temperature regime of berries at the beginning of crushing, to technologies for maceration and must purification, to new developments and protocols for yeast inoculation, yeast feeding and aging processes in different containers. …”
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Effect of the Application of Cold Plasma Energy on the Inactivation of Microorganisms, Proteins, and Lipids Deterioration in Adobera Cheese by Blanca Rosa Aguilar Uscanga, Montserrat Calderón Santoyo, Juan Arturo Ragazzo Sánchez, Mario Iván Alemán Duarte, Julia Aurora Pérez Montaño, Edgar Balcázar-López, Josué Raymundo Solís Pacheco

“…Plasma as a nonthermal inactivation technique has been a current research topic for food preservation, so the objective of this work was to study the effect of plasma energy against microorganisms in Adobera cheese (traditional Mexican cheese) as well as evaluate the possible degradation of lipids and protein. 108 CFU/mL of Escherichia coli ATCC 25922, Salmonella ATCC13076, and Staphylococcus aureus ATCC 6538 were inoculated at 0.5 g of Adobera cheese and were subjected to an energy of 30 volts, in a dielectric barrier discharge reactor (DBDR) at intervals of times 1, 3, 5, 7, 10, and 15 min. …”
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Effect of attenueted parapoxvirus ovis on levels of some serum cytokines and antibody response in sheep by Kamil Atlı, Atilla Şimşek

“…Serum samples were obtained from vena jugularis at 0. hour (control) before the inoculation and at the following 6., 12., 24., 48., 72., 96., 120. hours, 7., 14., 21., 30., 60., 90., 120., 150. and 180. days. …”
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Tissue-specific resistance and susceptibility to the tomato brown rugose fruit virus (ToBRFV) conferred by Solanum pennellii loci by Edan Rochsar, Shai Torgeman, Kfir Bandel, Amnon Koren, Chen Klap, Aviv Dombrovsky, Dani Zamir

“…Results The BILs were inoculated and genotyped for 5000 single primer enrichment technology (SPET) markers and phenotyped for virus presence, using ELISA, and for visual symptoms in the terminal shoot, axillary shoots, and fruits. …”
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Cultivation of Pleurotus ostreatus on Agricultural Wastes and Their Combination by Bulti Kumera Fufa, Belsti Atnkut Tadesse, Mestawot Merid Tulu

“…The cooled substrates were inoculated with a spoon of P. ostreatus spawn brought from Debre Berhan University. …”
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Bioremediation of Polycyclic Aromatic Hydrocarbon contaminated Aqueous-Soil matrix: Effect of co-contamination by CN Owabor, OC Onwuemene, I Enaburekhan

“…The soil was sterilized by heating at 120oC for one hour. 100g of the soil was contaminated with lead, chromium, nickel and mercury (40-200mg/l), 200mg/l of naphthalene and finally inoculated with microbes (Bacillus spp and Aspergillus niger). …”
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Leaf and tuber resistance to <I>Phytophthora infestans</I> and relationship between these traits in wild potato species by N. M. Zoteyeva, E. A. Porokhovinova, D. A. Fateev, N. A. Chalaya

“…Assessment of leaf and tuber resistance in 97 accessions belonging to 36 species was carried out under artificial inoculation. Using the data obtained on each individual plant, the connection between leaf and tuber resistance was analyzed by means of the Wilcoxon matched-pairs test and Spearman’s rank-order correlations.Results. …”
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Systemic Inflammatory Responses and Lung Injury following Hip Fracture Surgery Increases Susceptibility to Infection in Aged Rats by Hao Zhang, Tiansheng Sun, Zhi Liu, Jianzheng Zhang, Xiaowei Wang, Jia Liu

“…Both sham-operated and injured/surgical group animals underwent intratracheal inoculation with Pseudomonas aeruginosa 1, 3, and 7 days after surgery. …”
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Exogenous dsRNA triggers sequence-specific RNAi and fungal stress responses to control Magnaporthe oryzae in Brachypodium distachyon by Ying Zheng, Benjamin Moorlach, Desiree Jakobs-Schönwandt, Anant Patel, Chiara Pastacaldi, Stefan Jacob, Ana R. Sede, Manfred Heinlein, Minna M. Poranen, Karl-Heinz Kogel, Maria Ladera Carmona

“…The protective effects of dsRNA were further enhanced by maintaining a gap of at least seven days between dsRNA application and inoculation, and by stabilising the dsRNA in alginate-chitosan nanoparticles. …”
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Immunization of cattle with a Rhipicephalus microplus chitinase peptide containing predicted B-cell epitopes reduces tick biological fitness by María Martina Esperanza Perez-Soria, Daniel Gustavo López-Díaz, Rafael Jiménez-Ocampo, Gabriela Aguilar-Tipacamú, Massaro W. Ueti, Juan Mosqueda, Ala Tabor

“…The immunogenicity of each peptide was assessed by inoculating 2 cattle, 4 times at 21 days intervals, and the antibody response was verified by indirect ELISA. …”
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Screening of Lignocellulose-Degrading Superior Mushroom Strains and Determination of Their CMCase and Laccase Activity by Li Fen, Zhu Xuwei, Li Nanyi, Zhang Puyu, Zhang Shuang, Zhao Xue, Li Pengju, Zhu Qichao, Lin Haiping

“…In order to screen lignocellulose-degrading superior mushroom strains ten strains of mushrooms (Lentinus edodes939, Pholiota nameko, Lentinus edodes868, Coprinus comatus, Macrolepiota procera, Auricularia auricula, Hericium erinaceus, Grifola frondosa, Pleurotus nebrodensis, and Shiraia bambusicola) were inoculated onto carboxymethylcellulose agar-Congo red plates to evaluate their ability to produce carbomethyl cellulase (CMCase). …”
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Spireoside Controls Blast Disease by Disrupting Membrane Integrity of Magnaporthe oryzae by Xu Liting, He Kaiwei, Guo Chunyu, Quan Cantao, Ma Yahuan, Zhang Wei, Ren Lifen, Wang Long, Song Li, Ouyang Qing, Yin Junjie, Zhu Xiaobo, Tang Yongyan, He Min, Chen Xuewei, Li Weitao

“…We found that 24 and 48 h post-inoculation with Magnaporthe oryzae were critical time points for metabolomic profiling, based on the infected status of M. oryzae in rice and the observed differences in shikimate accumulation between the two varieties. …”
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