Splice-site mutation enhances the efficiency of CRISPR-mediated gene knockout and new germplasm creation in fish by Zhen Xu, Ma Zhuo, Leihui Wang, Shuo Yan, Shiyi Zhang, Ying Chen, Guang Xu, Chenxu Wang, Wenjing Tao, Deshou Wang

“…The knockout efficiency of the CRISPR/Cas9 system largely relies on the frameshift mutation caused by random insertions or deletions at target site of coding DNA sequence (CDS). Splice site (SS) mutations result in mRNA mis-splicing, making gRNAs targeting SS more likely to produce null mutations and thereby enhancing CRISPR efficiency. …”
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An insight into the draft genome of the Oriental rat flea, Xenopsylla cheopis, together with its Wolbachia endosymbiont by Stephen Lu, David M. Bland, Eric Dahlstrom, Neelam Redekar, Melina G. Guizzo, Kent Barbian, B. Joseph Hinnebusch, José M. C. Ribeiro

“…Results Sequencing data from both long and short reads were assembled into 7,694 contigs, from which 95,638 putative coding sequences (CDSs) were extracted and functionally annotated, providing insights into various aspects of flea physiology. …”
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Disagreement on estimating cause of death under tuberculosis treatment between the national tuberculosis registry and statistics data in South Korea, 2011–2020 by Chiwook Chung, Seung Won Lee, Dawoon Jeong, Hongjo Choi, Hojoon Sohn, Doosoo Jeon, Young Ae Kang

“…Age, sputum smear, and comorbidities were identifiable characteristics across groups that may influence the cause of death coding process.…”
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Predicting the impact of missense mutations on an unresolved protein’s stability, structure, and function: A case study of Alzheimer’s disease-associated TREM2 R47H variant by Joshua Pillai, Kijung Sung, Chengbiao Wu

“…Herein, we investigated Alzheimer's disease (AD)-causing coding variants of the triggering receptor expressed on myeloid cells 2 (TREM2) receptor using in-silico mutagenesis techniques on the AF2-predicted structure. …”
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Systematic Review of Patient and Caregiver Involvement in CKD Research by Talia Gutman, Dale Coghlan, Jonathan C. Craig, Chandana Guha, Allison Jaure, Shilpanjali Jesudason, Adeera Levin, David M. White, Javier Recabarren Silva, Anita van Zwieten, David Tunnicliffe, Andrea K. Viecelli, Germaine Wong, Armando Teixeira-Pinto, Siah Kim, Stephen McDonald, Carmel M. Hawley, Nicole Scholes-Robertson

“…Consumers were rarely drivers or commissioners of research (n = 6, 6%) and were most likely to be involved as informants (n = 81, 76%) with limited decision-making power. …”
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The emergence of common health conditions across the life course: evidence from the Born in Bradford family cohort [version 2; peer review: 2 approved] by John Wright, Gillian Santorelli, Rosemary R.C. McEachan, Krishnarajah Nirantharakumar, Dan Lewer, Katherine Phillips, Siang Ing Lee

“…Methods 21 health conditions were identified using diagnostic codes and prescription records extracted from electronic health records. …”
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Incidence of familial hypercholesterolemia in patients with early manifestations of coronary artery disease: data from a Russian multicenter study and meta-analysis by A. A. Rogozhina, A. O. Averkova, E. A. Zubova, L. O. Minushkina, V. A. Brazhnik, O. N. Ivanova, M. A. Chichkova, N. R. Khasanov, E. D. Kosmacheva, O. I. Boeva, D. A. Zateyshchikov

“…The use of existing diagnostic scales does not provide guaranteed detection of FH among patients with early CAD. It is likely that DLCN modification by additional gradation of the criterion for the age of CAD manifestation will help increase its diagnostic value.…”
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The biocontrol effects and mechanisms of mycoparasitic Alternaria alternata against Chinese rose powdery mildew by Yanping Tang, Yanping Tang, Ruotian Gao, Ruotian Gao, Changle Ma, Jin Wang, Jing Li, Jing Li

“…Up-regulated expression of KMRChis after induction is likely to play a role in disrupting the spore wall during mycoparasitic process of strain KMR13. …”
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Strengthening the Delivery of Physical Healthcare for Adults Living With Serious Mental Illness – A Qualitative Description of Patient and Family Member Perspectives by Munazzah Ambreen, Christopher Canning, Brian Lo, Sri Mahavir Agarwal, David Castle, Barna Konkolÿ‐Thege, Frank Sirotich, Sanjeev Sockalingam, Tania Tajirian, Philip G. Tibbo, Mary Rose vanKesteren, Caroline Walker, Vicky Stergiopoulos

“…Conclusions Findings from this study highlight the need for advancing the integration of physical healthcare within mental health settings for adults living with SMI, who are less likely to engage with community‐based primary care services. …”
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Facilitators and barriers to participation and scale-up of a non-specialist delivered psychological intervention for adolescents in low-resourced settings: a process evaluation by Alissa M. Terp, Rand Habashneh, Felicity L. Brown, Adnan Abualhaija, Ibrahim S. Aqel, Maha Ghatasheh, Richard Bryant, Mark J. D. Jordans, Aiysha Malik, Ellenor Mittendorfer-Rutz, Aemal Akhtar

“…Inductive and deductive codes were utilized in a six-step thematic analysis. …”
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Impact of Obstructive Sleep Apnea in Patients with Acute Heart Failure: A Nationwide Cohort Study by Abdulmajeed Alharbi, Nahush Bansal, Anas Alsughayer, Momin Shah, Waleed Alruwaili, Mohammed Mhanna, Halah Alfatlawi, Eun Seo Kwak, Ayman Salih, Mohanad Qwaider, Ragheb Assaly

“…The patients with OSA were more likely to be male, older in age and had a higher burden of comorbidities. …”
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Socioecological factors linked to co-occurring early childhood sleep health disparities and developmental outcomes: protocol for the sleep in preschoolers cross-sectional study by Darko Stefanovski, Ignacio E Tapia, Wanjikũ F M Njoroge, Rebecca Waller, Tiffani J Johnson, Eberechukwu A Uwah, Olivia Cicalese, Brizhay Davis, Megha Neelapu, Gabriel Steinberg, Arun Handa, Jodi A Mindell, Ariel A Williamson

“…Non-Latine Black/African American children are 4–6 times more likely than non-Latine White children to experience both SDB and short sleep duration. …”
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Psychological correlates of COVID-19 vaccine attitudes among medical professionals: An exploratory data analysis by Aldo SITIBONDO, Aanuoluwapo AFOLABI, Jamel HAJJI, Abdulmohsen Mohammed Abdullah ALKHULAYFI, Izaddin Ahmad AZIZ, Zane Asher GREEN, Francesca BRUNO, Martina BARBERA, Murat YLDIRIM

“…For statistical purposes, vaccination status was numerically coded (1 = fully vaccinated; 0.5 = first dose only; 0 = not vaccinated) and dichotomized into “Vaccinated” (full vaccination) versus “Unvaccinated” (partial or no vaccination). …”
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Exploring the factors that influence engagement with and experiences of integrated care for adults at risk of cardiovascular disease and mild-to-moderate mental health concerns in... by Alison R. McKinlay, Neil Howlett, Vivi Antonopoulou, Fabiana Lorencatto, Laura J. McGowan, David Osborn, Amy O’Donnell, Emily J. Oliver, Ivo Vlaev, Falko F. Sniehotta, Michael P. Kelly, Susan Michie, Caroline Kemp, Yannis Pappas, Gurch Randhawa, Nasreen Ali, Emily Munro, Angel M Chater

“…Where appropriate, thematic synthesis of extracted data will be coded to the Theoretical Domains Framework (TDF), updated Consolidated Framework for Implementation Research (CFIR) and Action, Actor, Context, Target and Time (AACTT) framework. …”
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Genetic diversity of the Duffy antigen/receptor for chemokines gene and its susceptibility to zoonotic malaria in non-human primates and Indonesian populations by Dendi H. Permana, Puji B. S. Asih, Dwi A. Suryandari, Elsa H. Murhandarwati, Isfanda Isfanda, Ismail E. Rozi, Lepa Syahrani, Farah N. Coutrier, Hannie Kartapradja, Hairul Afzan, Najdah Hidayah, Din Syafruddin

“…Notably, individuals with the FYA allele in Aceh, a region with high P. knowlesi incidence, were less likely to have an infection, suggesting a potential protective role. …”
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The Michigan men’s diabetes project: perspectives on a peer-led diabetes self-management and support intervention for Black men with type 2 diabetes by Alana M. Ewen, Jaclynn M. Hawkins, Katherine A. Kloss, Srijani Sengupta, Hannah Burgess, Robin Nwankwo, Martha Funnell, Jamie Mitchell, Gretchen Piatt

“…Abstract Background Black men are more likely to be diagnosed with type 2 diabetes (T2D) compared to non-Hispanic White men, especially those over 55 years of age. …”
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The agitated pediatric patient located in the emergency department: The APPLIED observational study by Matthias M. Manuel, Sing‐Yi Feng, Kenneth Yen, Faisalmohemed Patel

“…Non‐Hispanic Blacks were more likely to be agitated (adjusted odd ratio [aOR], 1.8; 95% CI, 1.2–2.7), but not restrained (aOR, 0.8; 95% CI, 0.3–1.8). …”
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Forecasting stroke and stroke-driven dementia in a rapidly ageing population: a model-based analysis of alternative projection scenarios for Ireland by Anne Hickey, Eithne Sexton, Frances Horgan, Kathleen E Bennett, Ronan Collins, Martin O'Flaherty, Suzanne Timmons, Chris Macey, David J. Williams

“…There is an urgent need to both invest in prevention strategies and plan for likely increases in future stroke care needs.…”
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Temporal Trends of Maternal Mortality Due to Obstetric Hemorrhage in Chinese Mainland: Evidence from the Population-Based Surveillance Data Between 2000 and 2019 by Yi Mu, Jun Zhu, Yanping Wang, Jiani Zhang, Mingrong Li, Peiran Chen, Yanxia Xie, Juan Liang, Xiaodong Wang, Dandan Shi

“…The final underlying cause of death was confirmed by the national review and was coded according to International Classification of Diseases -10. …”
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Health care utilization among Medicare beneficiaries with newly diagnosed back pain by Maria Isabel Barros Guinle, BA, Thomas Johnstone, BS, Gabriela D. Ruiz Colón, MD, Yingjie Weng, MHS, Ella A. Nettnin, BS, John K. Ratliff, MD

“…Patients were queried via ICD codes from a 20% random sample of Medicare claims records. …”
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