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  1. 6421
  2. 6422
  3. 6423

    Standardization and validation of a novel reverse transcriptase polymerase chain reaction method for detecting virulent strains of the infectious bursal disease virus by Vladimir Longa-Bobadilla, Phillip Ormeño-Vásquez, Manuel Criollo-Orozco, Luis Tataje-Lavanda, Katherine Huamán-Gutierrez, Ángela Montalván, Mirko Zimic, Manolo Fernández-Sanchez, Manolo Fernández-Díaz

    Published 2025-12-01
    “…Conclusion: Our novel RT-PCR procedure has been demonstrated to be helpful in selectively amplifying the consensus sequence of the VP5 gene, indicating that this novel RT-PCR procedure constitutes an important and useful tool to execute initial discrimination of field-retrieved samples containing and not containing virulent strains of this virus before deciding to execute a blindly and more costly sequencing procedure of all the samples together.…”
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  4. 6424

    Management of the mitral valve in thoracoscopic trans-mitral myectomy for hypertrophic obstructive cardiomyopathyCentral MessagePerspective by Tong Tan, MD, Jian Liu, MD, Bingqi Fu, MD, Peijian Wei, MD, Wei Zhu, MD, Jian Zhuang, MD, PhD, Jimei Chen, MD, PhD, Huiming Guo, MD

    Published 2023-12-01
    “…Conclusions: Total TTM in selected patients is safe and effective, and all 3 MV management strategies can significantly reduce the LVOTG while improving MR. …”
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  5. 6425

    Factors driving the variation in soil fertility associated with extension of the alfalfa (Medicago sativa L.) cultivation period by Zeyu Liu, Junqi Wang, Xinhe Shan, Pinhang Liang, Lizhao Fan, Chengyu Song, Zhaopu Jia, Yuxia Zhang, Yunlong Zhang, Lei Liu, Ding Huang

    Published 2025-12-01
    “…In topsoil, soil macro-aggregates and the mean weight diameter (MWD) showed an initial decrease and then a gradual increase with the number of planting years. …”
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  6. 6426

    Parental perceptions of a wearable vital signs monitor for observation of newborns: in-depth interviews in three tertiary hospitals, Southwest Nigeria by Yetunde Olufisayo John-Akinola, Adejumoke Idowu Ayede, Ayomide Adeyemi, Assumpta Nantume, Adesewa Oluwatomisin Olaleye, Olugbenga Akinrinoye, Olukemi Oluwatoyin Tongo, Michael Abe Alao, Adenike Oluleye, Oluseye Sobande, Olatayo Sunday Olayemi, Bertha Akinyi Oketch, Rosena Olubanke Oluwafemi, Rosena Olubanke Oluwafemi, Patricia Ireti Eniowo, Samuel Ojo, Omolayo Adebukola Olubosede, Emmanuel Olaseinde Bello, Ismaila Sani, Teresa Cauvel, Sona Shah

    Published 2025-07-01
    “…In-depth interviews were conducted with 17 parents/guardians whose newborns were monitored using the neoGuard technology. Participants were selected based on specific criteria, including observation of at least 2 h of use of the neoGuard technology on their newborn, and interviews were conducted in the participants’ preferred language. …”
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  7. 6427
  8. 6428

    Dysfunctional terminal differentiation of satellite cells in the wooden breast muscle of Ross 308 broiler chickens by Xin Wang, Tong Xing, Xudong Zhu, Xiaofei Wang, Liang Zhao, Feng Gao, Lin Zhang

    Published 2025-05-01
    “…A total of 100 Ross 308 broilers (42 days old) fed the same diet were randomly selected. Initial identification of normal and WB phenotypes was conducted through behavioral assessments and manual palpation of the PM muscle. …”
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    Article
  9. 6429

    Effectiveness of CONUT and NRI as nutritional risk screening tools in peritoneal dialysis: a multicenter study by Wenlong Qiu, Wenlong Qiu, Rui Chu, Rui Chu, Yaqi Ma, Yaqi Ma, Jiaxin Xue, Jiaxin Xue, Qingdong Xu, Yueqiang Wen, Xiaojiang Zhan, Fenfen Peng, Xiaoyang Wang, Juan Wu, Ning Su, Xiaoran Feng, Xingming Tang, Xianfeng Wu, Qian Zhou, Na Tian, Na Tian

    Published 2025-05-01
    “…This study investigates whether different nutritional assessment methods, like the Controlling Nutritional Status (CONUT) score and Nutritional Risk Index (NRI), correlate with patient prognosis, highlighting the importance of selecting appropriate screening tools to improve clinical outcomes in PD patients.MethodsThis multicenter retrospective cohort study initially collected data from 2,427 patients across 10 centers, but ultimately included a cohort of 2,105 PD patients to evaluate the prevalence of malnutrition assessed using both the CONUT and NRI and its independent effects on all-cause mortality. …”
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  10. 6430

    The influence of resveratrol and β-Hydroxy-β-methyl butyric acid supplementation alone or in combination on the development and health of the duodenum in Tibetan sheep by Yu Zhang, Yu Zhang, Qiurong Ji, Qiurong Ji, Linsheng Gui, Linsheng Gui, Kefyalew Gebeyew, Shengzhen Hou, Zhiyou Wang, Lijuan Han, Chao Yang

    Published 2025-07-01
    “…A total of 120 early weaned Tibetan rams with similar initial body weight (15.5 ± 0.14 kg) were selected and randomly allocated into 4 groups. …”
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  11. 6431

    Protocol for the practice guideline for traditional Chinese medicine preventive treatment on insomnia disorder by Yuting Duan, Yuting Duan, Yuting Duan, Yuting Duan, Yuting Duan, Yu Wang, Haichun Yang, Haichun Yang, Haichun Yang, Haichun Yang, Yuejuan Cai, Shengwei Wu, Pinge Zhao, Sande Gao, Enyi Liu, Enyi Liu, Peijing Rong, Lin Yu, Lin Yu, Lin Yu, Lin Yu, Lin Yu

    Published 2025-04-01
    “…Therefore, to improve the rehabilitation and quality of life of patients with insomnia disorder and its high-risk groups, the Affiliated TCM Hospital of Guangzhou Medical University and the Institute of Acupuncture and Moxibustion, CACMS have jointly initiated the compilation of the “Practice guideline for TCM preventive treatment of Insomnia disorder”.MethodsThe guideline will adhere to the principles outlined in WHO Handbook for Guidelines Development, 2nd Edition by the World Health Organization and the Guidelines for the Development of Traditional Chinese Medicine by the Chinese Society of Traditional Chinese Medicine. …”
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  12. 6432

    Application of point-of-care ultrasound-guided tip navigation combined with visualised directional high-frequency linear array probe compression to improve the success rate of umbi... by Fa Chen, Xianping Liu, Yunfeng Lin, Yanfang Fan, Shaoru Huang, Wen Ling, Jiajia Lin, Xia Ouyang

    Published 2025-03-01
    “…While point-of-care ultrasound (POCUS)-based tip navigation and location has improved this scenario by enabling real-time visualisation of the catheter tip during UVC, challenges remain when the catheter is inadvertently inserted into an incorrect vessel. Selecting effective intervention methods to correct the catheter direction has become a key research focus. …”
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  13. 6433

    Rapid diagnosis of Mycoplasma pneumoniae and prediction of antibiotic resistance by nanopore adaptive sampling by Yunke Sun, Xiaonan Li, Jiale He, Lingguo Zhao, Qingliang Chen, Lei Lei, Jun Chen, Lin Zhong, Guobao Li, Yu Xia, Yanmin Bao, Yingdan Zhang, Liang Yang

    Published 2024-12-01
    “…Nanopore adaptive sampling (NAS) aims to selectively enrich pathogens, promising improved diagnostics for acute infections and better treatment decisions in clinical practice. …”
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  14. 6434

    Development and validation of a polyfit approach for assessing alveolar bone loss using panoramic radiography by Erkang Tian, Jiawei Hong, Zihua Tang, Ruiting Ren, Shuoshun Li, Abbas Ahmed Abdulqader, Mingshan Li, Chaoran Xue, Xianglong Han, Juan Li

    Published 2025-03-01
    “…This model aids in Periodontal Screening and Recording, serving as a novel, valuable and intuitive tool for initial periodontal diagnosis.…”
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  15. 6435

    Characterization and correlation analysis of oral NET markers and inflammatory factor levels in patients after orthodontic treatment: a pilot study by Qian Liu, Axian Wang, Donghui Guo, Houzhuo Luo, Shishu Fang, Zhixin Song, Yi Wen, Fang Jin

    Published 2025-05-01
    “…In this study, we describe the changes in the levels of neutrophil extracellular traps (NET) markers and inflammatory factors in the gingival crevicular fluid (GCF) and saliva of patients after orthodontic treatment and further explore the correlation between them.Methods68 patients underwent fixed orthodontic treatment in the Department of Orthodontics from January 2021 to June 2023 were selected. GCF and saliva samples were collected from the patients 1 day before orthodontic treatment and 2 h, 24 h, and 1 week after orthodontic treatment to evaluate changes in NET marker and inflammatory factors. …”
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  16. 6436

    Radiomics with Clinical Data and [<sup>18</sup>F]FDG-PET for Differentiating Between Infected and Non-Infected Intracavitary Vascular (Endo)Grafts: A Proof-of-Concept Study by Gijs D. van Praagh, Francine Vos, Stijn Legtenberg, Marjan Wouthuyzen-Bakker, Ilse J. E. Kouijzer, Erik H. J. G. Aarntzen, Jean-Paul P. M. de Vries, Riemer H. J. A. Slart, Lejla Alic, Bhanu Sinha, Ben R. Saleem

    Published 2025-08-01
    “…The best-performing models for the <i>MAGIC-light</i> features and <i>PET-radiomics</i> features were selected from 343 unique models. Most relevant features were combined to test three final models using ROC analysis, accuracy, sensitivity, and specificity. …”
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  17. 6437

    Subnational introduction of the RTS,S/AS01E malaria vaccine into routine immunization: experience and lessons from the three pilot countries by Rose Jalang’o, Kwame Amponsa-Achiano, Mike Chisema, Keziah Malm, Lydiah Khalayi, Brenda Mhone, Wahjib Mohammed, Franklin Asiedu-Bekoe, Adam Haji, Josephine Njoroge, Boston Zimba, Esther Chirwa, Peter O. Tweneboah, Jackson Sillah, Mgaywa G. M. D. Magafu, Cynthia Bergstrom, Tracey Goodman, Jenny Walldorf, Kristen Kelleher, Eliane Pellaux-Furrer, Mary J. Hamel, Michael R. Adjei, Rafiq N. A. Okine

    Published 2025-07-01
    “…Methods Pilot areas were identified, in part, based on local malaria epidemiology. RTS,S was initially introduced in randomly selected areas, while other areas served as comparators until the four-dose schedule vaccine was scaled up following the WHO recommendation in 2021. …”
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    Article
  18. 6438

    Quantitative cervicovaginal fluid fetal fibronectin: A liquid biopsy for intra‐amniotic inflammation by Puntabut Warintaksa, Roberto Romero, Waranyu Lertrat, Nutnaree Yuenyongdechawat, Paninee Mongkolsuk, Supakorn Chaiyakarn, Rapeewan Settacomkul, Pisut Pongchaikul, Pornpun Vivithanaporn, Piya Chaemsaithong

    Published 2024-11-01
    “…This test may be useful as an initial assessment test to select appropriate patients for amniocentesis to determine intra‐amniotic inflammation.…”
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  19. 6439

    Development of an explainable prediction model for portal vein system thrombosis post-splenectomy in patients with cirrhosis by Caixia Dong, Guodong Li, Rui Zhou, Dou Qu, Duwei Dai, Junxia Zhao, Lingbo An, Xiaojie Song, Jiazhen Zhu, Zong Fang Li

    Published 2025-04-01
    “…Important features for predicting PVST included albumin, platelet addition, the diameter of the portal vein, γ-glutamyl transferase, length of stay, activated partial thromboplastin time, D-dimer level and history of preoperative gastrointestinal bleeding, as revealed by SHAP analysis.Conclusions The machine learning-based prediction models can provide an initial assessment of 3-month PVST risk after SPDV in patients with cirrhosis and portal hypertension. …”
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    Article
  20. 6440

    Effects of Energy Intake on Nutrient Digestibility, Nitrogen Metabolism, Energy Utilization, Serum Biochemical Indices, and Rumen Microbiota in Lanzhou Fat-Tailed Sheep by Wangmei Feng, Juanshan Zheng, Na Jiao, Chi Ma, Honghe Li, Junsong Zhang, Jutian Yang, Hongwei Xu, Yong Cai, Dandan Gao, Xin Cao, Xiaofang Feng, Penghui Guo

    Published 2025-03-01
    “…A total of twenty rams, each aged eight months and with an initial mean body weight of 27.81 ± 3.38 kg, were selected and randomly assigned to one of four experimental groups. …”
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    Article