Enhancing the reverse transcriptase function in Taq polymerase via AI-driven multiparametric rational design by Yulia E. Tomilova, Nikolay E. Russkikh, Igor M. Yi, Elizaveta V. Shaburova, Viktor N. Tomilov, Galina B. Pyrinova, Svetlana O. Brezhneva, Olga S. Tikhonyuk, Nadezhda S. Gololobova, Dmitriy V. Popichenko, Maxim O. Arkhipov, Leonid O. Bryzgalov, Evgeniy V. Brenner, Anastasia A. Artyukh, Dmitry N. Shtokalo, Dmitry N. Shtokalo, Dmitry N. Shtokalo, Denis V. Antonets, Mikhail K. Ivanov, Mikhail K. Ivanov

“…These candidates, along with the wild type, were assessed in the wet lab for multiple properties to establish an initial training dataset. 2) Using embeddings of Taq polymerase variants generated by a protein language model, we trained a Ridge regression model to predict multiple enzyme properties. …”
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Explainable machine learning model for predicting the transarterial chemoembolization response and subtypes of hepatocellular carcinoma patients by Yunjie Zhang, Songjian Tong, Junhui Yang, Jiawei Lin, Yifan Kong, Deyu Lu, Yan Chen, Yingchao Li, Linfeng Xu, Xiuyan Kong, Guoqing Zhu, Hao Zhang, Pixu Liu, Zhijie Yu, Jinglin Xia

“…We developed a Machine Learning (ML)-based model integrating routine clinical variables to preoperatively predict TACE efficacy, enabling tailored TACE candidate selection and optimized therapeutic decision-making. …”
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Re-resection of brain metastases – outcomes of an institutional cohort study and literature review by David Wasilewski, Zoe Shaked, Annalena Fuchs, Siyer Roohani, Ran Xu, Max Schlaak, Nikolaj Frost, Martin Misch, David Capper, David Kaul, Julia Onken, Peter Vajkoczy, Felix Ehret

“…Further research to improve patient selection and treatment algorithms is warranted.…”
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Anew yellow-flesh nectarine cultivar Zhongnong Zhenzhu 4 by WANG Yingying, LIU Lichang, LIU Zhiwu, YANG Xingwang, WANG Xiaodi

“…A total of 105 hybrid seeds were produced from this cross. Initial selection took place in 2016, focusing on its excellent eating quality, appealing appearance, and high yield potential. …”
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The Effect of Intravenous Autologous Activated Platelet-Rich Plasma Therapy on “Profibrotic Cytokine” IL-1β Levels in Severe and Critical COVID-19 Patients: A Preliminary Study... by Karina Karina, Louis Martin Christoffel, Rita Novariani, Imam Rosadi, Iis Rosliana, Siti Rosidah, Siti Sobariah, Novy Fatkhurohman, Nurlaela Puspitaningrum, Yuli Hertati, Irsyah Afini, Difky Ernanda, Tias Widyastuti, A. D. Sulaeha, Alfida Zakiyah, Noor Aini, Grady Krisandi, Hubert Andrew

“…Severe and critical patients’ initial plasma IL-1β concentration was 4.71 pg/mL and 3.095 pg/mL, respectively. …”
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Development and Validation of a Nomogram Prediction Model for In-hospital Mortality in Patients with Cardiac Arrest: A Retrospective Study by Peifeng Ni, Shurui Xu, Weidong Zhang, Chenxi Wu, Gensheng Zhang, Qiao Gu, Xin Hu, Ying Zhu, Wei Hu, Mengyuan Diao

“…Variables of interest included demographics, comorbidities, CA-related characteristics, vital signs, and laboratory results, and the outcome was defined as in-hospital death. Variables were selected using least absolute shrinkage and selection operator (LASSO) regression, recursive feature elimination (RFE), and eXtremely Gradient Boosting (XGBoost). …”
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Transcatheter Ductus Arteriosus Closure with Various Devices in the Pediatric Patient Group and Long-term Outcomes: Experience from a Single Center by Şeyma Şebnem Ön, Eser Doğan, Fırat Ergin, Mehmet Baki Beyter, Gülçin Kayan Kaşıkçı, Meral Yılmaz, Burcu Büşra Acar, Burcugül Karasulu Beci, Zülal Ülger Tutar, Reşit Ertürk Levent

“…The early closure rate was 97.5%, with initial shunt rates of 0.6% and 0.3% at one month. …”
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Archeohandi: protocol for a national disabilities database in archaeology in France by Rozenn Colleter, Valérie Delattre, Cyrille Le Forestier, Alex Baiet, Philippe Blanchard, Fanny Chenal, Anne-Sophie Coupey, Stéphanie Desbrosse-Degobertière, Sylvie Duchesne, Cécile Durin, Jean-Luc Gisclon, Noémie Gryspeirt, Fanny La Rocca, Raphaëlle Lefebvre, Jérôme Livet, Cécile Paresys, Mikaël Rouzic, Isabelle Souquet, Florence Tane, Aminte Thomann, Ivy Thomson, Émilie Trébuchet, Marie-Cécile Truc, Jean-Baptiste Barreau

“…A preliminary category selection of disabling pathologies has been made. These are trepanation, completely edentulous and/or compensating denture, neuronal impairment, severe scoliosis, Paget's disease, Diffuse Idiopathic Skeletal Hyperostosis (DISH), rickets, dwarfism, infectious diseases, unreduced fracture, amputation, severe degenerative disease and others. …”
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Sociological Explanation of Factors Affecting the Prevalence of Child Marriage (Case Study: Dishmok City) by Nasrollah Nosratikhah, Seyed Samad Beheshty, Maryam Mokhtari

“…Data were collected using a researcher-developed questionnaire administered to a sample of 355 individuals selected through a multi-stage random sampling technique. …”
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Assessment of Circulating Tumor Cells in Colorectal Cancer as an Adjunctive Non-invasive Diagnostic Method by Saskia Aziza Nursyirwan, Murdani Abdullah, Andri Sanityoso Sulaiman, Ikhwan Rinaldi, Dadang Makmun, Marcellus Simadibrata, Dimas Ramadhian Noor, Agustinus Wiraatmadja, Wifanto Saditya Jeo, Nur Rahadiani, Diah Rini Handjari, Hamzah Shatri

“…Biopsy remains the gold standard for CRC diagnosis, but invasive testing may not be preferred as an initial diagnostic procedure. Therefore, alternative non-invasive approaches are needed. …”
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Cone-beam CT measurement of root canal diameter and taper for mandibular first molar by LI Wenmiao, XING Li, PAN Yingyu, HUANG Ying, YANG Guofang, LIU Deda

“…At several levels of most root canals, the tapers in the <50-year-old groups were significantly larger than those in the 50‒59-year-old group (P<0.05).Conclusion·Mandibular first molars exhibit age-related narrowing of root canal diameter and reduced taper in individuals aged ≥50 years. For initial apical file selection, #15 files are recommended for the distal canals in the three-canal type, while #10 files are advised for all other canals in the three-canal type and all canals in the four-canal type. …”
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Análisis comparativo de los perfiles motivacionales y el Estado de Flow entre una metodología tradicional y la metodología Flipped Classroom en estudiantes de Educación Física (Co... by Pablo Cuenca Ruano, Salvador García Martínez, Alberto Ferriz Valero, Juan Tortosa Martínez

“…To determine if there are initial and final differences between the groups, the T-Student test was performed on independent samples. …”
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Co-creating a person-centered creative engagement intervention for Parkinson's care by Blanca T. M. Spee, Blanca T. M. Spee, Blanca T. M. Spee, Thieme B. Stap, Thieme B. Stap, Marjoke Plijnaer, Gert Pasman, Sara Zeggio, Annelien Duits, Annelien Duits, Julia S. Crone, Suzanne Haeyen, Suzanne Haeyen, Matthew Pelowski, Matthew Pelowski, Bastiaan R. Bloem, Jan-Jurjen Koksma

“…., duration of the intervention and sessions), (ii) freedom in selection of creative media, (iii) environment as a creative playground, (iv) skills of creative therapists, (v) PD-specific considerations, (vi) financial considerations and logistics, and we list (vii) responsibilities of the Design Team.DiscussionThis study establishes an initial framework for a PD-specific creative arts therapy intervention designed as a creative engagement learning environment. …”
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Sensitivity studies of a four-dimensional local ensemble transform Kalman filter coupled with WRF-Chem version 3.9.1 for improving particulate matter simulation accuracy by J. Lin, J. Lin, T. Dai, L. Sheng, W. Zhang, S. Hai, Y. Kong

“…This study advances our understanding of the selection of basic parameters in the 4D-LETKF assimilation system and the performance of ensemble simulations in a particulate-matter-polluted environment.…”
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Characterization of dynamic compliance of the respiratory system in healthy anesthetized dogs by Mathieu Raillard, Martina Mosing, Martina Mosing, Anthea Raisis, Adam Auckburally, Georgina Beaumont, Frances Downing, Charlotte Heselton, Paul MacFarlane, Karine Portier, Karine Portier, Josephine Robertson, Joao Henrique Neves Soares, Barbara Steblaj, Elliot Wringe, Olivier L. Levionnois

“…Multicollinearity (variables highly correlated with each other) was addressed by investigating, transforming, or excluding factors. Initial simple linear regression assessed each variable's individual effect on Cdyn, followed by a multiple linear regression model constructed via stepwise forward selection and backward elimination.ResultsThe best-fitting model identified a linear relationship between Cdyn and body mass when the following conditions were met: high BCS (Body Condition Score), orotracheal tubes &lt;7% smaller than predicted, the use of a D-lite flow sensor, and the absence of a high FIO2 (&gt;80%) exposure for more than 10 minutes before Cdyn measurement. …”
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Statistical Optimisation of <i>Streptomyces</i> sp. DZ 06 Keratinase Production by Submerged Fermentation of Chicken Feather Meal by Samir Hamma, Nawel Boucherba, Zahra Azzouz, Marilize Le Roes-Hill, Ourdia-Nouara Kernou, Azzeddine Bettache, Rachid Ladjouzi, Rima Maibeche, Mohammed Benhoula, Hakim Hebal, Zahir Amghar, Narimane Allaoua, Kenza Moussi, Patricia Rijo, Said Benallaoua

“…This study focused on the isolation of actinobacteria capable of producing extracellular keratinase from keratin-rich residues, which led to the selection of an actinobacterial strain referenced as <i>Streptomyces</i> strain DZ 06 (ES41). …”
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Development, Validation, and Deployment of a Time-Dependent Machine Learning Model for Predicting One-Year Mortality Risk in Critically Ill Patients with Heart Failure by Jiuyi Wang, Qingxia Kang, Shiqi Tian, Shunli Zhang, Kai Wang, Guibo Feng

“…Least Absolute Shrinkage and Selection Operator (LASSO) regression was employed to select predictive features from an initial pool of 64 candidate variables (including demographic characteristics, vital signs, comorbidities and complications, therapeutic interventions, routine laboratory data, and disease severity scores). …”
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Improving mechanical properties of maraging steel by optimizing laser power in hybrid laser-arc directed energy deposition by Jikang Fan, Pengfei Gao, Baihao Cai, Gaige Chang, Mengwei Duan, Wei Zhang, Xiaoyong Zhang, Yong Peng, Kehong Wang

“…Among these factors, the selection and optimization of laser power are particularly crucial for enhancing material properties. …”
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Maternal plasma cell-free RNA as a predictive test for fetal lung maturation by Sean W. D. Carter, Kay Yi Michelle Seah, Si En Poh, Winston Koh, Haruo Usuda, Erin L. Johnson, Yusaku Kumagai, Tsukasa Takahashi, Lara J. Monteiro, Reyna Peñailillo, Gino Nardocci, Hannah R. S. Watson, Masatoshi Saito, Mahesh A. Choolani, Sebastián E. Illanes, Matthew W. Kemp

“…Conclusions Further development of this technology may provide a rapid, minimally invasive, and cost-effective clinical tool to optimize patient selection for initial and repeat courses of antenatal steroids, along with insights into the molecular mechanisms underlying fetal lung development. …”
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The impact of delayed treatment of uncomplicated P. falciparum malaria on progression to severe malaria: A systematic review and a pooled multicentre individual-patient meta-analys... by Andria Mousa, Abdullah Al-Taiar, Nicholas M Anstey, Cyril Badaut, Bridget E Barber, Quique Bassat, Joseph D Challenger, Aubrey J Cunnington, Dibyadyuti Datta, Chris Drakeley, Azra C Ghani, Victor R Gordeuk, Matthew J Grigg, Pierre Hugo, Chandy C John, Alfredo Mayor, Florence Migot-Nabias, Robert O Opoka, Geoffrey Pasvol, Claire Rees, Hugh Reyburn, Eleanor M Riley, Binal N Shah, Antonio Sitoe, Colin J Sutherland, Philip E Thuma, Stefan A Unger, Firmine Viwami, Michael Walther, Christopher J M Whitty, Timothy William, Lucy C Okell

“…<h4>Methods and findings</h4>A search using Ovid MEDLINE and Embase was initially conducted to identify studies on severe Plasmodium falciparum malaria that included information on treatment delay, such as fever duration (inception to 22nd September 2017). …”
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